Standard name
Human Ortholog
Description Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 – 0 0 0 0 0.05 0.09 0.1 0.1 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.05 0.08 – 0.22 0.17 0.24 0.17 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0.88 0.89 0.94 0.91 0.88 0.86 0.85 – 0.74 0.71 0.63 0.7 0.9 0.83 0.82 0.81 0.83 0.83 0.84 0.86 0.84 0.64 0.66 0.67
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.43 0.21 0.05 0.06 0.11 0.15 0.2 – 0.34 0.29 0.37 0.29 0.2 0.16 0.15 0.07 0.1 0.06 0.1 0.07 0.09 0.23 0.23 0.19
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 1 0 0 2 2 6 – 10 1 5 8 0 0 0 3 0 1 0 1 2 1 3 6
Bud Neck 0 0 0 0 2 0 5 – 4 6 3 10 0 0 0 0 0 0 0 0 0 0 3 4
Bud Site 0 0 1 1 2 6 14 – 15 10 8 15 2 2 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 6 2 16 11 13 19 – 15 15 19 6 29 59 86 63 33 33 0 0 0 0 0 2
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 1 1 0 1 10 6 7 0 0 0 1 1 2
Endosome 0 1 3 0 0 1 0 – 0 0 0 0 0 0 3 7 2 3 0 1 2 2 1 4
Golgi 0 0 0 1 0 1 0 – 0 0 0 0 0 1 0 4 0 2 0 0 0 0 3 2
Mitochondria 5 12 5 6 8 23 46 – 125 89 135 99 16 11 17 13 3 23 0 2 2 1 3 2
Nucleus 76 322 341 289 390 373 480 – 427 370 348 411 540 567 670 505 292 339 84 328 337 190 332 394
Nuclear Periphery 1 0 1 0 2 3 5 – 3 5 1 9 4 6 8 3 2 3 0 1 0 0 1 2
Nucleolus 37 76 18 20 50 66 115 – 196 152 208 169 118 112 127 41 35 25 10 26 36 69 116 114
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 2 3 6 2 0 0 0 0 0 1 0 0
SpindlePole 1 2 8 2 9 7 8 – 9 3 3 15 3 4 7 6 7 10 1 3 2 0 2 6
Vac/Vac Membrane 0 2 5 2 3 2 2 – 2 8 9 1 1 2 10 15 8 13 2 12 11 24 28 36
Unique Cell Count 86 361 362 316 443 434 567 576 521 556 591 602 685 820 623 353 406 101 382 400 296 503 587
Labelled Cell Count 123 423 384 337 479 498 700 806 659 739 744 716 767 936 672 388 459 101 382 400 296 503 587


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 7.7 7.6 7.1 7.1 6.2 6.1 – 5.5 5.6 5.4 5.5 7.3 7.0 6.8 9.5 9.9 9.1 6.8 6.8 7.2
Std Deviation (1e-4) 1.3 1.4 1.1 1.3 1.1 1.3 1.4 – 1.6 1.3 1.3 1.2 1.9 1.8 1.9 1.8 1.9 2.0 1.4 1.5 1.6
Intensity Change (Log2) – – – -0.1 -0.11 -0.31 -0.33 – -0.47 -0.45 -0.51 -0.46 -0.07 -0.13 -0.16 0.32 0.38 0.25 -0.17 -0.16 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.6 – 2.7 0 0 2.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.6 2.2 2.5 2.8 – 2.3 2.5 2.8 0 3.6 5.3 6.0 5.8 5.4 5.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.5 3.0 4.4 – 8.8 7.4 9.4 7.4 1.3 0.3 0.8 0.8 0 3.2
Nucleus -1.4 -3.0 -3.8 -4.4 – -7.8 -8.6 -10.8 -9.1 -2.4 -5.2 -5.6 -5.7 -4.8 -4.6
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 3.2 4.7 6.5 – 10.3 9.0 11.1 8.9 6.3 5.3 5.1 1.0 2.5 0.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 -0.2 -0.6 -0.9 – -0.7 0 0 0.3 0 0 0 -1.6 -0.2 0.2
Vacuole 0 0 0 0 – 0 0.2 0.3 0 0 0 0 1.1 0.9 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.325 3.4471 3.9818 3.5175 3.7235 3.8371 6.5465 5.8177 6.1699 5.9323 6.0158 6.0492 4.3851 4.6269 4.1163 4.3402 4.6778 4.204
Actin 0 0 0 0 0 0 0 0 0 0 0 0 0.0137 0 0.0136 0 0 0.0088
Bud 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0003 0.0001 0 0 0.0001 0.0001
Bud Neck 0 0.0001 0 0 0.0002 0.0004 0 0.0001 0 0 0.0001 0.0002 0.0008 0.0002 0.0001 0 0.0003 0.0004
Bud Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.0003 0.0003 0 0 0.0001 0.0001
Bud Site 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0026 0.0002 0.0002 0 0.0001 0.0001
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0 0.0015 0 0 0 0 0 0.0001 0 0 0 0 0.0026 0 0.0013 0 0 0
Cytoplasmic Foci 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0053 0 0.0006 0 0 0.0009
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0 0 0.0001
Endoplasmic Reticulum 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0013 0 0.0012 0 0 0.0002
Endosome 0 0.0007 0 0 0 0 0 0 0 0 0 0 0.0079 0.0001 0.0015 0 0 0.0018
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.0015 0 0.0002 0 0 0.0019
Lipid Particles 0 0 0 0 0 0 0 0 0 0 0 0 0.0038 0 0.0005 0 0 0.0037
Mitochondria 0 0.0001 0 0.0001 0 0 0 0 0 0 0.0001 0 0.0011 0.0002 0.0002 0 0.0008 0.0006
None 0 0.0005 0 0 0 0 0 0 0 0 0 0 0.0019 0 0.0003 0 0.0001 0.0002
Nuclear Periphery 0.0008 0.008 0.0004 0.0009 0.0004 0.0001 0.0002 0.0023 0.0004 0.0001 0.0018 0.0002 0.0187 0.001 0.0098 0.0001 0.0015 0.001
Nucleolus 0.0368 0.0494 0.0214 0.0139 0.2877 0.1584 0.0199 0.0247 0.0129 0.0132 0.0885 0.0969 0.0341 0.0202 0.0272 0.0083 0.2021 0.0875
Nucleus 0.9581 0.9318 0.978 0.9837 0.7062 0.8403 0.9779 0.9703 0.9845 0.9865 0.9029 0.902 0.8945 0.9762 0.9383 0.9915 0.7935 0.889
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.001 0 0.0005 0 0 0.0025
Punctate Nuclear 0.0042 0.0062 0.0002 0.0012 0.0053 0.0005 0.0019 0.0021 0.0021 0.0002 0.0065 0.0005 0.0067 0.0002 0.0037 0.0001 0.0013 0.001
Vacuole 0 0.0011 0 0 0 0.0001 0 0.0002 0 0 0 0 0.0009 0.0009 0.0004 0 0.0001 0.0001
Vacuole Periphery 0 0.0003 0 0 0 0 0 0.0001 0 0 0 0 0.0006 0.0002 0.0002 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2789 21.0534 15.2902 22.982 20.79 11.0179 19.249 20.1755 19.9459 21.5522
Translational Efficiency 1.8268 1.3242 1.5834 1.1885 1.1458 1.8629 1.0855 1.0252 1.0327 1.1036

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
822 1525 718 1109 2133 1258 1717 2190 2955 2783 2435 3299

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 664.21 851.22 1063.07 1020.08 831.46 887.46 981.31 989.66 784.94 867.60 1005.42 999.89
Standard Deviation 88.50 174.62 151.03 162.15 111.47 135.70 153.20 227.70 129.48 159.24 157.05 208.48
Intensity Change Log 2 — 0.357893 0.678525 0.618971 — 0.094035 0.239062 0.251286 — 0.217201 0.450871 0.426217

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000189 0.000578 0.000948 0.001288 0.000250 0.000582 0.000907 0.000862 0.000233 0.000580 0.000919 0.001005
Bud Neck 0.001850 0.007710 0.014342 0.041006 0.006349 0.008200 0.016488 0.026670 0.005097 0.007931 0.015855 0.031489
Bud Site 0.000543 0.001295 0.001035 0.006235 0.000400 0.000882 0.001276 0.002715 0.000440 0.001108 0.001205 0.003899
Cell Periphery 0.000104 0.000098 0.000026 0.000195 0.000084 0.000142 0.000049 0.000261 0.000089 0.000118 0.000042 0.000239
Cytoplasm 0.003087 0.002132 0.000032 0.002663 0.000313 0.000972 0.000313 0.005885 0.001084 0.001608 0.000230 0.004801
Cytoplasmic Foci 0.000375 0.000383 0.000020 0.002720 0.000210 0.000931 0.000183 0.001691 0.000256 0.000630 0.000135 0.002037
Eisosomes 0.000022 0.000043 0.000074 0.000057 0.000039 0.000047 0.000057 0.000043 0.000034 0.000045 0.000062 0.000047
Endoplasmic Reticulum 0.001513 0.002243 0.001577 0.002809 0.001807 0.002187 0.002042 0.002301 0.001725 0.002218 0.001905 0.002472
Endosome 0.000120 0.000573 0.000048 0.003395 0.000155 0.000987 0.000212 0.001241 0.000145 0.000760 0.000164 0.001965
Golgi 0.000075 0.000313 0.000115 0.003645 0.000128 0.000459 0.000150 0.000879 0.000113 0.000379 0.000140 0.001809
Lipid Particles 0.000097 0.000206 0.000032 0.000428 0.000053 0.000785 0.000051 0.000202 0.000065 0.000468 0.000045 0.000278
Mitochondria 0.000489 0.001420 0.001895 0.008452 0.000557 0.001193 0.001588 0.003351 0.000538 0.001317 0.001678 0.005065
Mitotic Spindle 0.000203 0.000969 0.000386 0.038813 0.000417 0.003387 0.003111 0.010597 0.000357 0.002062 0.002307 0.020082
None 0.014184 0.005703 0.000664 0.004889 0.001588 0.001077 0.002484 0.008151 0.005092 0.003612 0.001948 0.007054
Nuclear Periphery 0.000144 0.000133 0.000127 0.003774 0.000101 0.000199 0.000186 0.001365 0.000113 0.000163 0.000169 0.002175
Nuclear Periphery Foci 0.000228 0.000355 0.000053 0.001822 0.000066 0.000431 0.000152 0.000662 0.000111 0.000390 0.000123 0.001052
Nucleolus 0.109475 0.095796 0.119789 0.055579 0.079647 0.108244 0.095098 0.039835 0.087944 0.101423 0.102378 0.045128
Nucleus 0.866461* 0.877529* 0.855865* 0.812695* 0.906880* 0.867055* 0.873828* 0.888630* 0.895637* 0.872795* 0.868531* 0.863103*
Peroxisomes 0.000330 0.001474 0.002250 0.000995 0.000428 0.001112 0.001066 0.000534 0.000401 0.001310 0.001416 0.000689
Vacuole 0.000279 0.000587 0.000505 0.005084 0.000301 0.000522 0.000496 0.002518 0.000294 0.000558 0.000499 0.003381
Vacuole Periphery 0.000233 0.000461 0.000216 0.003455 0.000230 0.000607 0.000263 0.001608 0.000231 0.000527 0.000249 0.002228

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.88 -10.78 -18.78 -13.18 -5.51 -4.68 -11.15 -8.84 -3.50 0.18 -9.23 -14.79 -15.99 -8.81 -2.42
Bud Neck -8.86 -14.86 -18.83 -16.13 -12.01 -2.99 -14.21 -18.69 -16.18 -8.23 -6.42 -18.97 -26.09 -23.03 -13.68
Bud Site -1.65 -2.09 -7.06 -6.01 -6.67 -2.56 -6.71 -10.80 -7.23 -5.33 -2.99 -6.67 -11.81 -8.33 -8.42
Cell Periphery 0.23 4.01 -1.70 -1.96 -5.05 -0.53 0.71 -1.73 -0.66 -2.60 -0.51 1.34 -2.00 -1.27 -3.51
Cytoplasm 0.85 2.98 0.34 -0.82 -6.06 -0.97 -0.08 -6.76 -4.22 -6.77 -0.89 2.83 -5.67 -4.06 -8.22
Cytoplasmic Foci -0.45 2.67 -2.12 -1.86 -2.97 -1.17 0.45 -2.43 -0.69 -2.78 -1.31 1.10 -3.15 -1.74 -3.85
Eisosomes -10.61 -8.59 -12.90 -5.12 2.23 -3.28 -9.03 -1.92 1.68 7.14 -6.22 -12.15 -8.14 -1.93 5.72
Endoplasmic Reticulum -4.10 -0.63 -8.26 -5.59 -6.06 -2.58 -1.95 -3.09 -0.97 -1.03 -4.39 -1.97 -6.95 -3.66 -4.08
Endosome -2.03 3.10 -3.92 -2.45 -4.06 -1.71 -0.59 -3.77 -0.14 -3.40 -2.48 -0.30 -5.42 -1.64 -5.21
Golgi -2.55 -4.29 -2.73 -2.37 -2.65 -1.40 -0.28 -2.48 -0.91 -2.54 -2.09 -0.52 -3.63 -2.60 -3.59
Lipid Particles -1.18 3.22 -1.68 -0.64 -2.17 -1.21 0.32 -2.00 1.01 -2.43 -1.46 1.08 -2.59 0.87 -3.18
Mitochondria -3.09 -4.86 -5.65 -4.53 -3.97 -3.04 -5.70 -6.30 -4.59 -3.43 -3.92 -7.40 -8.33 -6.15 -5.28
Mitotic Spindle -0.69 -0.22 -7.77 -7.68 -7.79 -2.55 -3.21 -6.72 -3.60 -4.16 -2.64 -3.18 -10.20 -8.74 -8.45
None 3.62 6.27 4.29 1.00 -4.58 1.34 -0.94 -5.89 -7.29 -4.66 1.82 4.08 -1.11 -3.19 -5.81
Nuclear Periphery 0.33 -0.35 -4.68 -4.70 -4.64 -2.13 -3.16 -5.02 -4.55 -4.55 -1.94 -2.89 -6.83 -6.61 -6.48
Nuclear Periphery Foci -0.77 2.71 -2.70 -2.40 -3.33 -1.70 -0.66 -2.86 -0.69 -2.67 -2.21 0.41 -3.97 -2.24 -4.23
Nucleolus 2.55 -1.97 7.79 7.00 9.37 -6.92 -4.41 12.24 16.24 13.92 -4.36 -4.86 13.79 17.97 16.38
Nucleus -1.76 2.01 5.34 7.52 3.58 8.00 8.39 5.29 -2.75 -2.54 6.17 8.13 8.12 2.64 0.56
Peroxisomes -2.46 -11.46 -8.49 1.02 6.73 -1.97 -4.44 -0.93 1.69 6.52 -2.93 -9.02 -3.11 2.00 8.99
Vacuole -1.40 -1.15 -6.38 -5.86 -5.82 -0.90 -1.09 -5.20 -4.63 -4.74 -1.51 -1.47 -8.04 -7.32 -7.41
Vacuole Periphery -1.30 -0.00 -2.94 -2.52 -2.99 -1.71 -0.43 -3.54 -1.95 -3.46 -2.22 -0.37 -4.60 -3.31 -4.57
Standard name
Human Ortholog
Description Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Set2

Set2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Set2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available