Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 3 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 3 | 2 | 2 | 3 | 7 | 1 | 5 | 6 | 5 | 7 | 7 | 0 | 1 | 2 | 89 | 71 | 34 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 233 | 126 | 181 | 148 | 164 | 220 | 211 | 179 | 202 | 238 | 241 | 194 | 160 | 188 | 209 | 241 | 207 | 203 | 244 | 141 | 181 | 74 | 171 | 154 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 9 | 9 | 8 | 28 | 8 | 0 | 1 | 0 | 8 | 13 | 22 | 0 | 0 | 0 | 2 | 4 | 7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 0 | 1 | 2 | 1 |
Unique Cell Count | 235 | 127 | 181 | 149 | 165 | 224 | 212 | 191 | 218 | 251 | 278 | 208 | 160 | 191 | 210 | 287 | 250 | 222 | 251 | 147 | 186 | 84 | 186 | 173 |
Labelled Cell Count | 237 | 129 | 183 | 150 | 168 | 228 | 213 | 193 | 221 | 254 | 283 | 210 | 160 | 192 | 211 | 338 | 293 | 261 | 251 | 147 | 186 | 84 | 186 | 173 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 340.7 | 337.5 | 292.3 | 254.4 | 249.3 | 229.8 | 236.7 | 218.8 | 211.9 | 203.2 | 188.9 | 220.9 | 321.5 | 354.5 | 369.7 | 523.8 | 500.8 | 439.2 | 286.4 | 318.1 | 349.3 |
Std Deviation (1e-4) | 70.7 | 75.2 | 63.0 | 68.1 | 54.9 | 56.9 | 54.7 | 52.4 | 63.4 | 61.1 | 57.6 | 72.1 | 72.9 | 85.5 | 97.2 | 138.8 | 151.5 | 146.5 | 70.2 | 72.0 | 99.6 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.23 | -0.35 | -0.3 | -0.42 | -0.46 | -0.52 | -0.63 | -0.4 | 0.14 | 0.28 | 0.34 | 0.84 | 0.78 | 0.59 | -0.03 | 0.12 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.0 | 7.5 | 5.0 |
Cytoplasm | -1.1 | -1.0 | -1.8 | -0.9 | -3.4 | -3.7 | -3.1 | -5.1 | -3.6 | 0 | -1.7 | -0.9 | -5.7 | -5.9 | -4.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 | 0 | 0 | 0 | 0 | 3.1 | 4.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 247.7904 | 365.8219 | 331.0484 | 279.12 | 323.892 | 331.2065 | 324.4983 | 456.684 | 383.6641 | 352.3952 | 324.964 | 374.2412 | 282.853 | 399.1479 | 342.4086 | 285.4149 | 326.2491 | 423.9381 |
Actin | 0.0033 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0028 | 0.0001 | 0.0007 | 0.0012 | 0.0066 | 0.0002 | 0.0019 | 0.0002 | 0.0008 | 0.0008 | 0.0004 | 0.0002 |
Bud | 0.0021 | 0.0001 | 0.0012 | 0.0002 | 0.0003 | 0.0002 | 0.0011 | 0.0001 | 0.001 | 0.0002 | 0.0034 | 0.0006 | 0.0008 | 0.0003 | 0.0009 | 0.0004 | 0.0011 | 0.0001 |
Bud Neck | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0008 | 0.0011 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0003 |
Bud Periphery | 0.001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0059 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0 |
Bud Site | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0006 | 0.0001 | 0.0014 | 0.0001 | 0.0009 | 0 |
Cell Periphery | 0.004 | 0.0026 | 0.0007 | 0.0008 | 0.0003 | 0.0011 | 0.0016 | 0.0001 | 0.0005 | 0.0012 | 0.0014 | 0.0017 | 0.0012 | 0.0001 | 0.0013 | 0.001 | 0.0014 | 0 |
Cytoplasm | 0.7963 | 0.9757 | 0.9554 | 0.8252 | 0.927 | 0.9254 | 0.8449 | 0.9938 | 0.9339 | 0.9134 | 0.7783 | 0.9727 | 0.8854 | 0.9835 | 0.9577 | 0.8623 | 0.898 | 0.9877 |
Cytoplasmic Foci | 0.0127 | 0.0001 | 0.0077 | 0.0457 | 0.0097 | 0.0004 | 0.0083 | 0.0001 | 0.0088 | 0.0087 | 0.0043 | 0.0023 | 0.002 | 0.0002 | 0.0022 | 0.0003 | 0.0143 | 0.0002 |
Eisosomes | 0.001 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0015 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0035 | 0.0001 | 0.0009 | 0.0002 | 0.0122 | 0.0012 | 0.0017 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 |
Endosome | 0.0035 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0036 | 0 | 0.0049 | 0.0002 | 0.0233 | 0.0019 | 0.0009 | 0 | 0.0004 | 0.0006 | 0.0019 | 0 |
Golgi | 0.0008 | 0 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0003 | 0.0004 | 0.0022 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Lipid Particles | 0.0027 | 0.0001 | 0.0014 | 0.0079 | 0.0015 | 0.0001 | 0.0022 | 0 | 0.001 | 0.0015 | 0.0014 | 0.0002 | 0.0007 | 0 | 0.0006 | 0.0001 | 0.0017 | 0 |
Mitochondria | 0.0182 | 0.0005 | 0.0039 | 0.0224 | 0.0027 | 0.0028 | 0.017 | 0.0002 | 0.0067 | 0.037 | 0.0409 | 0.0001 | 0.005 | 0.0015 | 0.0048 | 0.0257 | 0.0119 | 0.0003 |
None | 0.0142 | 0.0011 | 0.0033 | 0.0051 | 0.0179 | 0.0009 | 0.0099 | 0.0003 | 0.0121 | 0.0093 | 0.0224 | 0.0006 | 0.003 | 0.0013 | 0.0047 | 0.0008 | 0.0059 | 0.0003 |
Nuclear Periphery | 0.0276 | 0.0035 | 0.0033 | 0.0125 | 0.0052 | 0.0049 | 0.0336 | 0.001 | 0.0083 | 0.0038 | 0.0292 | 0.0034 | 0.0442 | 0.0006 | 0.0026 | 0.0432 | 0.002 | 0.0019 |
Nucleolus | 0.0193 | 0.0025 | 0.0093 | 0.0444 | 0.0099 | 0.0156 | 0.0171 | 0.0001 | 0.0042 | 0.0069 | 0.016 | 0.0002 | 0.0064 | 0.005 | 0.0047 | 0.0041 | 0.0116 | 0 |
Nucleus | 0.0662 | 0.0093 | 0.0089 | 0.0229 | 0.0199 | 0.0273 | 0.0375 | 0.0037 | 0.0096 | 0.0105 | 0.0223 | 0.0044 | 0.0313 | 0.006 | 0.012 | 0.0308 | 0.0099 | 0.0085 |
Peroxisomes | 0.0056 | 0 | 0.0003 | 0.0023 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0009 | 0 |
Punctate Nuclear | 0.0017 | 0.0001 | 0.0004 | 0.0006 | 0.001 | 0.0005 | 0.0039 | 0 | 0.0012 | 0.0004 | 0.0011 | 0.0003 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0001 |
Vacuole | 0.011 | 0.0025 | 0.0016 | 0.0043 | 0.0011 | 0.0117 | 0.0041 | 0.0002 | 0.0023 | 0.0025 | 0.0124 | 0.0077 | 0.0047 | 0.0006 | 0.0022 | 0.0046 | 0.0228 | 0.0001 |
Vacuole Periphery | 0.0056 | 0.0003 | 0.0009 | 0.0037 | 0.0011 | 0.0068 | 0.0052 | 0.0001 | 0.0023 | 0.0016 | 0.0147 | 0.0018 | 0.0084 | 0.0002 | 0.0013 | 0.024 | 0.0136 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1457.6608 | 1901.4985 | 2317.0988 | 2096.006 | 1879.1137 | 1531.3107 | 1941.6739 | 2077.539 | 2467.6115 | 1782.5861 |
Translational Efficiency | 1.6911 | 1.6312 | 1.4993 | 1.8437 | 1.6451 | 1.3479 | 1.4698 | 1.6549 | 1.3589 | 1.6221 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2078 | 909 | 1332 | 1124 | 1828 | 779 | 1141 | 17 | 3906 | 1688 | 2473 | 1141 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 18117.87 | 20309.75 | 30040.48 | 46516.54 | 17615.65 | 20063.54 | 39532.58 | 51141.09 | 17882.83 | 20196.13 | 34419.97 | 46585.44 |
Standard Deviation | 4192.85 | 5210.82 | 6492.14 | 10115.62 | 3730.86 | 4804.07 | 7642.08 | 7493.05 | 3991.19 | 5028.70 | 8487.50 | 10097.11 |
Intensity Change Log 2 | — | 0.164759 | 0.729495 | 1.360330 | — | 0.187718 | 1.166184 | 1.537625 | — | 0.176123 | 0.961251 | 1.450455 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000024 | 0.000082 | 0.000073 | 0.000385 | 0.000031 | 0.000085 | 0.000117 | 0.000104 | 0.000027 | 0.000083 | 0.000093 | 0.000381 |
Bud Neck | 0.000103 | 0.000614 | 0.001661 | 0.001066 | 0.000208 | 0.000583 | 0.000938 | 0.001879 | 0.000152 | 0.000600 | 0.001327 | 0.001078 |
Bud Site | 0.001036 | 0.001975 | 0.000520 | 0.003899 | 0.000576 | 0.001048 | 0.001204 | 0.000516 | 0.000821 | 0.001547 | 0.000835 | 0.003848 |
Cell Periphery | 0.002284 | 0.007959 | 0.004220 | 0.153921* | 0.003533 | 0.008957 | 0.045247 | 0.117494* | 0.002868 | 0.008420 | 0.023149 | 0.153378* |
Cytoplasm | 0.930855* | 0.837283* | 0.752028* | 0.210782* | 0.903015* | 0.831308* | 0.665363* | 0.219214* | 0.917826* | 0.834526* | 0.712042* | 0.210908* |
Cytoplasmic Foci | 0.002146 | 0.009552 | 0.009230 | 0.002560 | 0.003457 | 0.012102 | 0.002447 | 0.004181 | 0.002759 | 0.010729 | 0.006100 | 0.002584 |
Eisosomes | 0.000000 | 0.000002 | 0.000003 | 0.000056 | 0.000001 | 0.000002 | 0.000012 | 0.000032 | 0.000001 | 0.000002 | 0.000007 | 0.000055 |
Endoplasmic Reticulum | 0.000082 | 0.000037 | 0.000031 | 0.000120 | 0.000028 | 0.000079 | 0.000041 | 0.000012 | 0.000056 | 0.000056 | 0.000036 | 0.000119 |
Endosome | 0.000032 | 0.000082 | 0.000060 | 0.001031 | 0.000039 | 0.000134 | 0.000028 | 0.000015 | 0.000035 | 0.000106 | 0.000045 | 0.001016 |
Golgi | 0.003032 | 0.005332 | 0.003079 | 0.000911 | 0.003538 | 0.004448 | 0.001202 | 0.000227 | 0.003269 | 0.004924 | 0.002213 | 0.000901 |
Lipid Particles | 0.000669 | 0.003646 | 0.002161 | 0.003145 | 0.001688 | 0.002776 | 0.007564 | 0.013437 | 0.001146 | 0.003244 | 0.004654 | 0.003299 |
Mitochondria | 0.000110 | 0.000208 | 0.000253 | 0.000357 | 0.000117 | 0.000171 | 0.000082 | 0.000013 | 0.000113 | 0.000191 | 0.000174 | 0.000352 |
Mitotic Spindle | 0.000267 | 0.001464 | 0.000343 | 0.000771 | 0.000637 | 0.000919 | 0.000061 | 0.000017 | 0.000440 | 0.001212 | 0.000213 | 0.000760 |
None | 0.009144 | 0.007927 | 0.004785 | 0.002065 | 0.010663 | 0.010478 | 0.002528 | 0.000097 | 0.009855 | 0.009105 | 0.003743 | 0.002036 |
Nuclear Periphery | 0.000074 | 0.000471 | 0.001540 | 0.000198 | 0.000338 | 0.000421 | 0.000495 | 0.000762 | 0.000197 | 0.000448 | 0.001058 | 0.000206 |
Nuclear Periphery Foci | 0.000996 | 0.002577 | 0.002196 | 0.000512 | 0.002325 | 0.002018 | 0.001808 | 0.001228 | 0.001618 | 0.002319 | 0.002017 | 0.000522 |
Nucleolus | 0.000035 | 0.000367 | 0.000094 | 0.000144 | 0.000054 | 0.000122 | 0.000061 | 0.000025 | 0.000044 | 0.000254 | 0.000079 | 0.000143 |
Nucleus | 0.040651 | 0.093160 | 0.181273* | 0.008021 | 0.056486 | 0.104486 | 0.061371 | 0.019697 | 0.048062 | 0.098387 | 0.125952 | 0.008195 |
Peroxisomes | 0.000007 | 0.000036 | 0.000003 | 0.000066 | 0.000005 | 0.000018 | 0.000010 | 0.000003 | 0.000006 | 0.000028 | 0.000007 | 0.000065 |
Vacuole | 0.004643 | 0.023212 | 0.032945 | 0.609045* | 0.009066 | 0.016885 | 0.207562* | 0.620556* | 0.006713 | 0.020292 | 0.113511 | 0.609217* |
Vacuole Periphery | 0.003811 | 0.004015 | 0.003502 | 0.000945 | 0.004195 | 0.002959 | 0.001858 | 0.000491 | 0.003991 | 0.003528 | 0.002744 | 0.000938 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.25 | -13.28 | -2.26 | -1.79 | -1.80 | -2.36 | -12.76 | -3.14 | -1.16 | -0.22 | -4.01 | -18.36 | -2.24 | -1.84 | -1.75 |
Bud Neck | -6.03 | -12.27 | -4.56 | -1.82 | 5.43 | -3.13 | -11.25 | -2.44 | -2.17 | -1.75 | -6.33 | -15.17 | -4.49 | -2.14 | 3.70 |
Bud Site | -2.21 | 1.33 | -2.96 | -1.75 | -3.21 | -1.93 | -3.66 | -0.86 | 0.53 | 1.18 | -2.76 | -1.38 | -3.15 | -2.30 | -3.00 |
Cell Periphery | -6.80 | -5.76 | -29.17* | -27.59* | -28.56* | -4.88 | -16.54 | -4.34 | -4.07 | -2.55 | -8.20 | -16.56 | -29.27 | -27.85 | -24.83 |
Cytoplasm | 15.15 | 31.52 | 88.92* | 60.00* | 43.47* | 10.64 | 25.91 | 10.80 | 9.43 | 6.55 | 18.30 | 40.35 | 88.75* | 67.73 | 46.98* |
Cytoplasmic Foci | -8.09 | -11.70 | -0.73 | 5.48 | 8.91 | -6.91 | 0.19 | -1.32 | -0.30 | -1.33 | -10.53 | -10.72 | -0.14 | 6.89 | 6.48 |
Eisosomes | -5.60 | -9.99 | -2.57 | -2.49 | -2.39 | -3.15 | -3.84 | -4.14 | -3.91 | -2.59 | -5.68 | -5.40 | -2.58 | -2.51 | -2.26 |
Endoplasmic Reticulum | 0.92 | 0.94 | -0.57 | -1.81 | -1.83 | -0.91 | -3.76 | 1.91 | 1.12 | 4.36 | -0.00 | 0.68 | -1.20 | -1.20 | -1.78 |
Endosome | -2.86 | -6.01 | -1.83 | -1.71 | -1.70 | -1.66 | 0.56 | 0.97 | 1.86 | 0.74 | -2.63 | -4.72 | -1.82 | -1.67 | -1.75 |
Golgi | -4.29 | -0.88 | 3.62 | 6.67 | 4.39 | -1.49 | 7.60 | 9.72 | 7.47 | 4.37 | -4.15 | 3.30 | 4.47 | 6.78 | 2.97 |
Lipid Particles | -6.23 | -7.38 | -7.52 | 0.74 | -0.65 | -2.22 | -8.39 | -2.34 | -2.17 | -1.25 | -6.16 | -10.61 | -6.53 | -0.47 | 4.15 |
Mitochondria | -2.02 | -10.59 | -2.18 | -0.98 | -0.56 | -1.61 | 1.56 | 5.31 | 3.98 | 6.16 | -2.55 | -7.69 | -2.15 | -1.24 | -1.36 |
Mitotic Spindle | -1.89 | -1.92 | -1.11 | 0.97 | -0.68 | -0.28 | 1.93 | 2.09 | 1.83 | 4.58 | -1.60 | 1.31 | -0.46 | 0.89 | -1.07 |
None | 1.78 | 8.57 | 7.20 | 5.65 | 2.71 | 0.29 | 10.88 | 14.35 | 11.73 | 10.04 | 1.36 | 13.80 | 8.36 | 7.14 | 1.68 |
Nuclear Periphery | -8.05 | -7.48 | -10.04 | 5.67 | 7.03 | -1.07 | -2.78 | -2.15 | -1.69 | -1.27 | -4.58 | -7.90 | -0.30 | 5.56 | 8.08 |
Nuclear Periphery Foci | -5.74 | -5.63 | 2.74 | 8.45 | 7.88 | 0.96 | 0.78 | 0.79 | 0.33 | 0.46 | -3.41 | -3.88 | 7.19 | 10.42 | 10.80 |
Nucleolus | -1.17 | -12.24 | -1.97 | 0.74 | -0.40 | -1.84 | -0.96 | 0.36 | 1.93 | 1.08 | -1.40 | -8.17 | -1.81 | 0.66 | -0.88 |
Nucleus | -12.71 | -29.77 | 12.55 | 19.21 | 34.66 | -10.67 | -4.27 | 0.85 | 3.58 | 1.78 | -16.51 | -25.87 | 17.15 | 26.30 | 33.92 |
Peroxisomes | -1.15 | 0.66 | -1.78 | -0.72 | -1.82 | -1.19 | -2.33 | 0.10 | 1.20 | 2.00 | -1.50 | -1.01 | -1.80 | -1.03 | -1.76 |
Vacuole | -7.80 | -13.36 | -76.72* | -69.35* | -65.14* | -3.69 | -25.38 | -9.10 | -8.94 | -5.79 | -8.41 | -26.69 | -76.91* | -72.78* | -54.16* |
Vacuole Periphery | -0.26 | 0.20 | 5.20 | 4.66 | 5.94 | 1.07 | 2.58 | 3.90 | 3.26 | 5.39 | 0.64 | 2.05 | 5.86 | 5.31 | 6.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|