ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 – 0.08 0.05 0 0 0.06 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.97 0.93 0.88 0.87 0.76 0.68 – 0.62 0.61 0.99 0.99 0.97 0.92 0.85 0.89 0.94 0.94 0.93 0.94 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.22 0.07 0.22 0.21 0.28 0.46 – 0.5 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.08 – 0 0.05 0 0 0 0.05 0.13 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 3 1 – 2 0 0 0 1 0 0 0 1 0 1 0 1 3
Bud 0 0 1 1 0 2 2 4 0 – 1 2 0 1 8 0 0 0 0 0 0 1 1 2
Bud Neck 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 8 1 0 8 4 6 13 3 – 8 9 1 8 20 18 14 14 0 0 0 0 0 0
Cytoplasm 176 302 105 111 210 126 191 240 115 – 63 102 256 335 340 363 197 310 177 297 106 154 236 235
Endoplasmic Reticulum 2 1 0 0 3 0 1 5 1 – 1 4 0 2 4 19 6 21 0 1 0 2 1 5
Endosome 0 0 0 0 1 0 0 0 0 – 1 0 0 2 0 1 1 1 3 3 0 0 0 2
Golgi 1 1 0 0 0 0 0 0 0 – 0 0 1 0 0 1 1 1 1 0 0 0 0 0
Mitochondria 1 6 7 25 16 32 47 88 78 – 50 90 0 1 8 2 2 5 1 4 1 1 1 5
Nucleus 2 0 1 0 1 0 2 5 0 – 1 1 1 0 3 9 2 7 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 1 0 0 2 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 9 3 4 9 13 – 2 9 0 2 3 20 31 40 0 4 1 2 2 2
Unique Cell Count 178 306 107 115 227 144 220 315 169 101 167 258 339 351 396 231 348 189 316 114 164 256 269
Labelled Cell Count 191 320 116 138 249 169 253 367 213 130 219 259 352 387 433 254 399 189 316 114 164 256 269


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.2 4.5 4.3 5.9 7.3 6.6 6.3 5.9 – 6.3 6.2 6.3 7.5 7.3 13.8 15.1 14.7 6.9 6.1 7.0
Std Deviation (1e-4) 0.8 1.3 0.8 1.7 1.8 9.4 2.4 1.9 2.1 – 2.4 2.5 2.7 2.7 2.7 3.4 6.1 5.2 2.9 1.6 2.1
Intensity Change (Log2) – – – -0.06 0.4 0.7 0.56 0.51 0.41 – 0.5 0.47 0.5 0.76 0.71 1.63 1.76 1.72 0.63 0.46 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -2.1 -3.1 -3.3 -5.1 -6.1 – -6.5 -7.0 0.9 0.5 -0.7 -2.3 -3.6 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 3.4 3.4 4.6 6.9 – 6.9 8.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 2.9 – 0 0 0 0 0 0 4.0 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7009 4.3299 4.7392 4.102 4.3065 4.4694 5.0277 5.5858 4.7478 4.4356 4.5988 4.9893 5.6972 6.3053 6.6113 5.2434 6.27 5.6569
Actin 0.1198 0.0046 0.0421 0.0041 0.0231 0.0033 0.1643 0.0094 0.008 0.026 0.0008 0.0257 0.1294 0.0063 0.0086 0.0894 0.0012 0.0012
Bud 0.0022 0.0004 0.0022 0.0014 0.0008 0.0002 0.0007 0.0026 0.0006 0.0032 0.0005 0.0035 0.0009 0.0009 0.0004 0.0068 0.0003 0.0002
Bud Neck 0.0373 0.0023 0.0024 0.0007 0.0008 0.0038 0.0044 0.0003 0.0004 0.0004 0.0002 0.0048 0.0028 0.0002 0.0038 0.001 0.0003 0.0006
Bud Periphery 0.0029 0.0003 0.0036 0.0031 0.001 0.0002 0.0009 0.0019 0.0004 0.003 0.0016 0.0037 0.0008 0.0008 0.0001 0.0054 0.0001 0.0001
Bud Site 0.0193 0.0083 0.0148 0.0029 0.0038 0.0002 0.0016 0.0126 0.0085 0.003 0.0004 0.0014 0.0125 0.0045 0.0016 0.0053 0.0002 0.0001
Cell Periphery 0.0017 0.0012 0.0009 0.0008 0.0003 0.0002 0.0006 0.0003 0.0004 0.0002 0.0002 0.0003 0.0009 0.0003 0.0002 0.0004 0.0001 0.0001
Cytoplasm 0.2369 0.7644 0.7575 0.7996 0.6266 0.8772 0.4849 0.8902 0.7917 0.7622 0.8218 0.8338 0.6724 0.9165 0.9179 0.7227 0.8421 0.8955
Cytoplasmic Foci 0.0427 0.0144 0.0269 0.0174 0.0497 0.0148 0.0071 0.0068 0.0141 0.0141 0.0072 0.0135 0.074 0.013 0.0132 0.0802 0.0204 0.0415
Eisosomes 0.0015 0.0003 0.0002 0.0002 0.0002 0 0.0006 0 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0714 0.0128 0.0195 0.0077 0.0069 0.0103 0.0607 0.0037 0.0033 0.0127 0.0133 0.0157 0.0113 0.0084 0.0064 0.0057 0.0055 0.0079
Endosome 0.0758 0.0129 0.0216 0.0488 0.0281 0.0248 0.0459 0.0033 0.0086 0.0238 0.0713 0.0216 0.0166 0.0053 0.0107 0.0252 0.0344 0.0126
Golgi 0.0232 0.0039 0.008 0.0025 0.0151 0.0022 0.0129 0.0012 0.005 0.0132 0.0038 0.0057 0.0164 0.0017 0.0015 0.0047 0.0013 0.0016
Lipid Particles 0.0533 0.0014 0.0084 0.0044 0.0336 0.0002 0.0033 0.0011 0.011 0.012 0.0074 0.0012 0.0073 0.0018 0.0006 0.0018 0.0015 0.0003
Mitochondria 0.0195 0.0006 0.003 0.0138 0.004 0.0006 0.0065 0.0012 0.0307 0.0062 0.0107 0.0024 0.0066 0.0005 0.0004 0.0009 0.0006 0.0004
None 0.0905 0.1601 0.0562 0.0462 0.1586 0.0565 0.0534 0.0473 0.0827 0.0924 0.0112 0.0325 0.0143 0.0184 0.0252 0.0352 0.0331 0.0322
Nuclear Periphery 0.0423 0.0018 0.007 0.0046 0.0037 0.0007 0.0612 0.0012 0.0098 0.0093 0.0221 0.021 0.0014 0.0014 0.0015 0.0018 0.0117 0.0005
Nucleolus 0.0087 0.0002 0.0006 0.0019 0.0005 0 0.0027 0.0046 0.0001 0.0002 0.0005 0.0003 0.0003 0.0001 0.0001 0.0001 0.0013 0
Nucleus 0.0765 0.0017 0.0029 0.0036 0.0012 0.0009 0.071 0.0085 0.0172 0.0028 0.003 0.0042 0.0008 0.0011 0.0015 0.0013 0.0074 0.0006
Peroxisomes 0.0318 0.0007 0.0117 0.0012 0.0333 0.0001 0.0014 0.0006 0.0035 0.0066 0.0006 0.0026 0.0267 0.0161 0.0006 0.0039 0.0007 0.0017
Punctate Nuclear 0.0248 0.0008 0.002 0.0006 0.0061 0.0003 0.0093 0.0007 0.0005 0.0019 0.0011 0.003 0.002 0.0011 0.003 0.0048 0.0323 0.0006
Vacuole 0.0139 0.0063 0.0071 0.0266 0.0023 0.0029 0.0042 0.0019 0.0028 0.0049 0.014 0.0019 0.0019 0.0013 0.0022 0.0026 0.0043 0.002
Vacuole Periphery 0.004 0.0005 0.0012 0.0079 0.0007 0.0006 0.0024 0.0004 0.0004 0.0016 0.008 0.0012 0.0003 0.0002 0.0005 0.0007 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.2116 6.9442 10.3058 15.9302 9.4148 14.8524 17.0952 16.7921 29.1999 18.0638
Translational Efficiency 0.7364 0.9197 0.6128 0.8348 0.7253 0.9977 0.554 0.5184 0.575 0.5746

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1044 1644 179 804 1165 1615 158 150 2209 3259 337 954

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 605.82 787.55 1302.32 1047.27 650.37 748.14 1134.47 1204.48 629.32 768.02 1223.62 1071.99
Standard Deviation 88.37 110.40 213.86 226.53 104.34 97.59 218.82 277.32 99.63 106.10 231.86 242.10
Intensity Change Log 2 — 0.378482 1.104123 0.789672 — 0.202048 0.802686 0.889078 — 0.289832 0.955927 0.841992

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000137 0.000272 0.000681 0.000204 0.000109 0.000630 0.000310 0.000666 0.000122 0.000450 0.000507 0.000277
Bud Neck 0.019655 0.024952 0.003387 0.003136 0.005640 0.035445 0.001433 0.002751 0.012264 0.030152 0.002471 0.003076
Bud Site 0.009549 0.019154 0.031143 0.012237 0.005273 0.025769 0.009066 0.028647 0.007294 0.022432 0.020792 0.014818
Cell Periphery 0.000194 0.000077 0.000242 0.000102 0.000138 0.000212 0.000256 0.000199 0.000164 0.000144 0.000248 0.000117
Cytoplasm 0.555078* 0.551941* 0.470255* 0.536193* 0.610886* 0.481639* 0.562983* 0.586110* 0.584511* 0.517102* 0.513730* 0.544042*
Cytoplasmic Foci 0.200058* 0.199272* 0.116934 0.046067 0.174922 0.238137* 0.048362 0.088864 0.186801* 0.218532* 0.084784 0.052796
Eisosomes 0.000258 0.000085 0.000040 0.000028 0.000088 0.000161 0.000038 0.000023 0.000168 0.000123 0.000039 0.000027
Endoplasmic Reticulum 0.001852 0.000358 0.002256 0.001035 0.000942 0.000441 0.001950 0.000550 0.001372 0.000399 0.002113 0.000959
Endosome 0.017781 0.022034 0.024772 0.015172 0.009419 0.033975 0.004275 0.009328 0.013371 0.027951 0.015162 0.014253
Golgi 0.002935 0.005075 0.001417 0.002426 0.001711 0.007402 0.000208 0.007284 0.002289 0.006228 0.000850 0.003190
Lipid Particles 0.007392 0.002720 0.004585 0.001376 0.005318 0.004193 0.001993 0.002352 0.006298 0.003450 0.003370 0.001530
Mitochondria 0.004602 0.004909 0.000070 0.000963 0.002944 0.007854 0.000070 0.004081 0.003727 0.006368 0.000070 0.001453
Mitotic Spindle 0.000370 0.010524 0.005060 0.007615 0.001451 0.013962 0.003317 0.005846 0.000940 0.012228 0.004243 0.007337
None 0.005472 0.001563 0.000323 0.001616 0.011398 0.001080 0.002646 0.000729 0.008597 0.001324 0.001412 0.001476
Nuclear Periphery 0.000235 0.000226 0.000368 0.000232 0.000513 0.000290 0.000548 0.000171 0.000382 0.000257 0.000452 0.000223
Nuclear Periphery Foci 0.000343 0.000377 0.010525 0.001010 0.000670 0.000401 0.004895 0.002334 0.000515 0.000389 0.007885 0.001218
Nucleolus 0.001248 0.001112 0.000122 0.000428 0.001225 0.000567 0.000108 0.000092 0.001236 0.000842 0.000115 0.000375
Nucleus 0.076634 0.050000 0.015433 0.043377 0.109575 0.036459 0.110816 0.005801 0.094007 0.043289 0.060153 0.037469
Peroxisomes 0.002468 0.007625 0.001113 0.001348 0.001466 0.010099 0.000371 0.002798 0.001940 0.008851 0.000765 0.001576
Vacuole 0.091929 0.096006 0.306686* 0.323525* 0.054460 0.098831 0.246223* 0.245788* 0.072168 0.097406 0.278338* 0.311302*
Vacuole Periphery 0.001809 0.001718 0.004590 0.001907 0.001851 0.002454 0.000133 0.005589 0.001831 0.002083 0.002500 0.002486

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.82 -4.58 -0.89 1.91 4.08 -2.74 -5.60 -1.76 0.83 -0.28 -3.41 -6.11 -2.02 2.22 4.03
Bud Neck -2.23 9.09 8.92 13.73 -0.23 -13.41 6.09 4.28 15.15 -1.54 -11.19 10.41 9.18 19.79 -1.58
Bud Site -5.51 -3.08 -1.18 3.81 2.72 -10.89 -1.91 -4.23 1.02 -3.21 -11.91 -3.48 -4.13 5.35 1.62
Cell Periphery 5.21 -1.00 4.65 -0.89 5.95 -1.42 -1.03 -0.38 1.02 0.66 0.72 -1.54 4.37 1.76 4.10
Cytoplasm 0.35 3.84 2.21 1.98 -2.39 12.29 5.96 2.71 -2.31 -2.16 8.95 7.07 4.93 -0.95 -3.21
Cytoplasmic Foci 0.15 9.79 26.08 27.86 7.55 -8.60 20.90 10.25 16.69 -4.47 -6.07 18.16 30.67 38.29 4.35
Eisosomes 2.66 3.32 3.57 12.95 4.11 -6.93 4.36 8.39 16.31 4.29 1.49 3.94 4.62 20.78 6.02
Endoplasmic Reticulum 2.47 -0.41 1.61 -2.34 3.46 2.55 -3.16 2.57 0.41 4.26 3.21 -2.45 1.79 -2.06 4.77
Endosome -2.31 -1.81 3.12 4.88 3.92 -12.66 6.08 2.03 13.06 -2.19 -11.02 -0.04 1.56 9.90 1.32
Golgi -3.12 4.81 1.69 3.73 -0.76 -7.65 3.95 -0.83 1.79 -1.59 -7.80 5.75 0.42 5.43 -1.98
Lipid Particles 5.35 2.98 7.65 3.19 4.71 2.07 6.39 3.28 2.01 -0.75 5.61 5.36 9.93 5.67 4.20
Mitochondria -0.19 4.98 4.23 5.30 -2.46 -3.23 2.96 0.55 2.98 -1.13 -2.70 5.47 3.89 7.18 -2.44
Mitotic Spindle -5.16 -2.64 -3.98 0.76 -1.47 -5.42 -0.89 -0.81 4.38 -0.03 -7.41 -2.50 -3.69 2.20 -1.88
None 4.25 5.73 4.51 0.74 -4.31 5.48 4.91 5.78 1.88 3.13 6.70 6.85 6.81 0.61 -0.25
Nuclear Periphery 0.16 -3.44 -0.31 -0.35 3.31 1.49 -3.60 3.78 0.88 7.61 1.55 -4.15 3.13 0.36 7.75
Nuclear Periphery Foci -0.69 -8.12 -3.93 -3.49 7.42 1.29 -6.15 -4.09 -4.63 3.47 1.08 -9.78 -4.06 -4.52 8.60
Nucleolus 0.33 4.31 1.97 1.42 -1.92 4.32 7.54 7.84 9.75 3.17 1.87 7.69 3.14 1.43 -1.87
Nucleus 6.58 15.71 8.35 3.16 -6.33 16.33 -1.10 21.66 11.39 7.71 16.50 3.15 15.82 3.21 4.27
Peroxisomes -8.59 5.50 4.28 10.34 -0.46 -12.65 8.65 -0.65 8.92 -2.57 -15.21 8.36 2.58 15.26 -2.47
Vacuole -1.28 -11.19 -22.77 -22.67 -0.86 -10.81 -12.66 -11.92 -9.88 0.46 -8.21 -16.99 -27.62 -24.97 -0.65
Vacuole Periphery 0.47 -0.73 1.06 0.83 0.88 -1.21 4.23 -0.37 -0.10 -1.18 -0.84 -0.08 0.42 0.99 0.23
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YMR196W

YMR196W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr196w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available