Standard name
Human Ortholog
Description Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.08 0.06 0.08 0.06 0 0.07 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.7 0.93 0.8 0.7 0.74 0.68 0.77 0.58 0.63 0.54 0.59 – – – 0.61 0.61 0.59 0.45 0.42 0.52 0.5 0.49 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.08 0 0.06 0 0 0 0 0 0.05
Endosome 0.06 0.12 0 0 0 0 0 0 0 0 0 0 – – – 0.1 0.16 0.16 0 0.05 0 0 0.26 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.15 0 0.08 0.08 0.16 0.33 0.2 0.53 0.32 – – – 0.06 0.07 0 0.1 0.06 0.19 0 0 0.05
Nucleus 0 0 0 0 0 0 0.05 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.11 0.06 0 0 0 0.17 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0.08 0.05 0.05 0.22 0.1 0.16 0.11 0.12 0.13 0.07 0.14 – – – 0.18 0.19 0.24 0.24 0.27 0.16 0.3 0.18 0.11
Vac/Vac Membrane 0 0.08 0 0 0 0.06 0 0 0 0 0 0 – – – 0.1 0.05 0.07 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 0 0 0 0 0 0 – – – 1 0 0 2 1 1 0 0 0
Bud 3 0 0 1 1 0 1 4 2 3 2 3 – – – 2 2 0 6 9 0 0 0 0
Bud Neck 1 3 1 1 12 7 14 10 3 12 4 10 – – – 1 3 1 2 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 1 1 3 – – – 0 0 1 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 1 0 1 – – – 1 1 3 1 0 0 0 0 0
Cytoplasm 196 138 53 63 110 87 119 134 65 111 122 142 – – – 141 141 127 135 100 45 1 3 8
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 2 0 0 1 – – – 19 9 14 2 0 0 0 0 1
Endosome 14 24 0 2 5 3 1 0 3 1 1 3 – – – 23 37 34 12 10 0 0 2 0
Golgi 0 1 0 1 0 0 0 0 0 0 0 0 – – – 2 0 1 2 2 0 0 0 0
Mitochondria 21 6 0 12 3 9 14 28 37 36 118 77 – – – 14 17 7 31 14 16 0 0 1
Nucleus 1 4 1 1 1 3 8 4 1 5 1 4 – – – 3 1 1 1 6 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 1 0 0 – – – 0 0 1 0 1 0 0 0 0
Nucleolus 11 2 1 3 0 0 4 5 0 3 6 8 – – – 6 25 13 5 4 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 2 0 0 0 0 0 0
SpindlePole 17 16 3 4 35 12 28 20 13 23 15 33 – – – 41 43 51 72 63 14 1 1 2
Vac/Vac Membrane 8 16 0 1 4 7 6 1 1 4 1 2 – – – 23 12 15 12 8 2 0 0 1
Unique Cell Count 250 197 57 79 158 117 176 175 112 176 224 241 233 231 217 302 236 87 4 8 21
Labelled Cell Count 272 215 59 89 171 130 195 207 127 201 271 287 277 291 271 302 236 87 4 8 21


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.4 4.9 4.7 5.5 4.4 4.5 4.6 3.4 4.0 3.2 3.5 – – – 5.5 5.4 5.4 – – –
Std Deviation (1e-4) 0.5 1.0 0.8 1.5 1.9 1.3 1.9 1.9 0.5 1.7 0.9 0.7 – – – 1.5 0.9 1.5 – – –
Intensity Change (Log2) – – – -0.08 0.15 -0.15 -0.12 -0.09 -0.54 -0.32 -0.62 -0.51 – – – 0.15 0.12 0.13 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6387 -0.7167 -0.6172 -0.9462 -0.3955 -0.9584 -1.0527 -0.2778 -0.1954 -0.2771 -0.325 -0.5645 -1.1789 -1.0459 -1.0625 -0.8775 -1.1575 -1.1868
Actin 0.0513 0.0007 0.0111 0.0147 0.0112 0.0032 0.0109 0.0001 0.0106 0.0156 0.0008 0.0087 0.0303 0.0009 0.0096 0.0009 0.0013 0.0119
Bud 0.0007 0.0006 0.0007 0.0066 0.0019 0.0005 0.0006 0.0001 0.0004 0.0021 0.0001 0.0002 0.0015 0.0026 0.0006 0.0001 0 0.0002
Bud Neck 0.0052 0.0011 0.0038 0.0354 0.0077 0.0023 0.0055 0.0004 0.0027 0.0201 0.0092 0.0048 0.006 0.0009 0.001 0.0101 0.0016 0.016
Bud Periphery 0.0012 0.0004 0.0012 0.0023 0.004 0.0009 0.0013 0.0002 0.0004 0.0036 0.0003 0.0005 0.0015 0.0011 0.0016 0.0001 0 0.0003
Bud Site 0.0158 0.007 0.0034 0.0011 0.0067 0.001 0.007 0.0015 0.0038 0.0072 0.0008 0.0002 0.0077 0.0117 0.001 0.001 0.0001 0.0005
Cell Periphery 0.0006 0.0001 0.0001 0.0009 0.0004 0.0002 0.0004 0.0001 0.0002 0.0004 0.0001 0.0001 0.0005 0.0005 0.0001 0.0001 0 0.0001
Cytoplasm 0.0328 0.0562 0.0372 0.0322 0.0339 0.0787 0.0144 0.028 0.0185 0.0099 0.0417 0.047 0.0405 0.0796 0.0358 0.0241 0.0618 0.1363
Cytoplasmic Foci 0.0803 0.045 0.0647 0.032 0.0338 0.0362 0.0804 0.0139 0.0849 0.0449 0.0385 0.0255 0.104 0.0771 0.0837 0.1564 0.0405 0.0346
Eisosomes 0.0011 0.0003 0.0002 0.0003 0.0004 0.0001 0.0007 0.0003 0.0004 0.0002 0.0016 0.0001 0.0006 0.0002 0.0004 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0037 0.0018 0.0018 0.0013 0.0079 0.0017 0.0021 0.0006 0.0005 0.0019 0.0011 0.0006 0.0031 0.0009 0.0011 0.0004 0.0122 0.0027
Endosome 0.0204 0.0052 0.0093 0.0095 0.0551 0.0061 0.0113 0.0016 0.01 0.0325 0.0053 0.0096 0.0263 0.0045 0.0124 0.0069 0.0087 0.0073
Golgi 0.0058 0.0006 0.0014 0.0073 0.0066 0.0036 0.0035 0.0002 0.0044 0.0102 0.0004 0.0049 0.0046 0.0006 0.0055 0.0011 0.0007 0.004
Lipid Particles 0.0348 0.0046 0.0098 0.0097 0.0075 0.0053 0.0357 0.0013 0.0136 0.034 0.0021 0.0024 0.029 0.005 0.0153 0.0141 0.0067 0.006
Mitochondria 0.0057 0.0021 0.0076 0.0062 0.0079 0.0038 0.0169 0.0003 0.0097 0.0129 0.0012 0.0088 0.0065 0.0008 0.0154 0.0007 0.0006 0.0018
None 0.6241 0.6388 0.303 0.1054 0.1511 0.677 0.6521 0.7333 0.3407 0.1181 0.3579 0.7553 0.623 0.6309 0.3588 0.2122 0.278 0.5775
Nuclear Periphery 0.0246 0.0042 0.0039 0.0054 0.0318 0.0073 0.0154 0.0019 0.0063 0.0107 0.0113 0.0029 0.0104 0.0025 0.0033 0.0054 0.0335 0.0043
Nucleolus 0.0041 0.011 0.0195 0.0146 0.0397 0.0054 0.0089 0.0059 0.0155 0.0254 0.041 0.0037 0.0045 0.0073 0.0066 0.0114 0.016 0.0087
Nucleus 0.0146 0.0449 0.0575 0.1053 0.2266 0.0648 0.0055 0.0246 0.0277 0.0233 0.1797 0.0523 0.0108 0.0158 0.0596 0.0774 0.1233 0.0628
Peroxisomes 0.0192 0.0021 0.0082 0.0025 0.0019 0.0023 0.0144 0.0006 0.0104 0.0585 0.0008 0.0047 0.0155 0.0017 0.0051 0.0068 0.0031 0.0031
Punctate Nuclear 0.049 0.1718 0.4482 0.6041 0.3509 0.0982 0.109 0.1838 0.4365 0.5532 0.3042 0.0657 0.07 0.1536 0.3812 0.4695 0.4097 0.1198
Vacuole 0.0029 0.0014 0.0061 0.0022 0.0086 0.0012 0.0022 0.0012 0.0023 0.0114 0.0016 0.0017 0.0027 0.0016 0.0013 0.0011 0.0014 0.0019
Vacuole Periphery 0.0023 0.0002 0.0014 0.0009 0.0043 0.0004 0.0015 0.0001 0.0004 0.0036 0.0004 0.0004 0.0009 0.0002 0.0005 0.0001 0.0006 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1995 8.8415 9.4311 10.052 11.2205 10.5028 19.1363 19.0436 13.287 16.8264
Translational Efficiency 0.7468 1.7209 0.8903 0.7685 0.7432 0.8777 0.7697 0.696 0.7769 0.6156

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
228 1284 97 1449 1593 1789 100 254 1821 3073 197 1703

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 644.03 654.75 956.86 836.15 671.60 698.65 918.71 871.06 668.15 680.31 937.49 841.36
Standard Deviation 78.39 89.06 113.04 113.71 76.09 115.60 94.62 117.08 76.93 107.53 105.83 114.89
Intensity Change Log 2 — 0.023816 0.571180 0.376634 — 0.056968 0.452007 0.375170 — 0.040834 0.511575 0.375887

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000094 0.000565 0.001273 0.001392 0.000085 0.000336 0.001029 0.001205 0.000086 0.000432 0.001149 0.001364
Bud Neck 0.004611 0.039799 0.001999 0.015528 0.009092 0.042481 0.001812 0.008396 0.008531 0.041360 0.001904 0.014465
Bud Site 0.001609 0.014580 0.002275 0.030416 0.002464 0.015807 0.002097 0.006053 0.002357 0.015294 0.002185 0.026782
Cell Periphery 0.000165 0.000206 0.000292 0.000287 0.000141 0.000218 0.000338 0.000161 0.000144 0.000213 0.000316 0.000268
Cytoplasm 0.398861* 0.287551* 0.163959* 0.134530* 0.511844* 0.357077* 0.128541* 0.120696* 0.497698* 0.328027* 0.145980* 0.132467*
Cytoplasmic Foci 0.160995 0.288011* 0.020788 0.008106 0.187723* 0.237039* 0.022344 0.005251 0.184377* 0.258336* 0.021578 0.007680
Eisosomes 0.000134 0.000164 0.000136 0.000051 0.000106 0.000143 0.000177 0.000059 0.000109 0.000152 0.000157 0.000052
Endoplasmic Reticulum 0.000223 0.000841 0.012938 0.005639 0.000460 0.000908 0.008357 0.007286 0.000430 0.000880 0.010613 0.005885
Endosome 0.007492 0.027122 0.006651 0.008163 0.006389 0.024207 0.006860 0.008194 0.006527 0.025425 0.006757 0.008167
Golgi 0.000973 0.010701 0.000187 0.009323 0.001318 0.006211 0.000238 0.000256 0.001275 0.008087 0.000213 0.007971
Lipid Particles 0.007757 0.020288 0.005270 0.001833 0.010641 0.014818 0.007138 0.001559 0.010280 0.017104 0.006218 0.001792
Mitochondria 0.001838 0.047131 0.000598 0.011072 0.002834 0.023033 0.000637 0.001081 0.002709 0.033102 0.000617 0.009582
Mitotic Spindle 0.001648 0.052976 0.002565 0.310425* 0.008844 0.031851 0.004859 0.302981* 0.007943 0.040677 0.003729 0.309314*
None 0.013766 0.014342 0.003229 0.012643 0.018386 0.009783 0.009124 0.016768 0.017808 0.011688 0.006221 0.013259
Nuclear Periphery 0.000443 0.000770 0.002751 0.004823 0.000719 0.001002 0.002310 0.002290 0.000684 0.000905 0.002527 0.004445
Nuclear Periphery Foci 0.000736 0.002975 0.005554 0.011263 0.002380 0.001500 0.010351 0.011646 0.002174 0.002116 0.007989 0.011320
Nucleolus 0.016185 0.013094 0.002861 0.012938 0.011881 0.011995 0.006156 0.014523 0.012420 0.012454 0.004534 0.013174
Nucleus 0.368626* 0.110421 0.673998* 0.383961* 0.205266* 0.151399* 0.694769* 0.448172* 0.225720* 0.134277* 0.684542* 0.393538*
Peroxisomes 0.004764 0.034975 0.000852 0.001510 0.005679 0.022663 0.001224 0.001802 0.005565 0.027807 0.001041 0.001553
Vacuole 0.008761 0.030372 0.091489 0.033399 0.013199 0.044904 0.091350 0.041391 0.012643 0.038832 0.091418 0.034591
Vacuole Periphery 0.000320 0.003117 0.000334 0.002698 0.000550 0.002626 0.000289 0.000230 0.000521 0.002832 0.000311 0.002330

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.87 -6.27 -14.82 -8.42 2.02 -4.21 -5.72 -6.69 -5.92 0.34 -5.62 -8.51 -17.10 -12.70 2.04
Bud Neck -11.65 -0.08 -11.23 4.52 -10.64 -14.16 5.01 -4.08 8.46 -7.08 -17.33 4.02 -10.94 8.14 -14.37
Bud Site -9.23 -3.61 -11.03 -5.33 -7.43 -9.21 -3.28 -6.62 -1.50 -3.59 -12.39 -4.49 -11.81 -5.41 -8.07
Cell Periphery -1.31 -5.01 -2.22 -0.98 3.78 -2.99 -7.00 -2.61 0.31 5.12 -3.74 -9.01 -4.42 -0.61 6.05
Cytoplasm 3.76 8.71 13.80 21.00 1.69 11.76 23.51 35.71 25.78 2.84 15.43 25.16 45.20 35.60 2.61
Cytoplasmic Foci -4.90 11.68 12.51 42.94 2.51 -3.91 28.80 35.26 43.40 4.75 -7.04 32.57 37.52 60.70 5.00
Eisosomes -1.22 -2.35 4.08 8.25 5.42 -2.40 -5.53 0.28 2.63 5.63 -3.20 -6.20 2.24 9.27 8.21
Endoplasmic Reticulum -6.95 -8.68 -21.46 -19.85 4.86 -1.85 -6.90 -9.86 -9.37 1.62 -3.15 -10.92 -23.10 -21.31 5.09
Endosome -5.75 -0.47 0.72 12.97 2.70 -12.42 -2.55 1.20 9.12 2.72 -15.63 -2.32 3.57 18.52 4.66
Golgi -9.06 1.98 -1.40 6.27 -2.51 -8.25 2.55 3.50 10.58 0.46 -12.00 4.09 -0.89 6.53 -2.23
Lipid Particles -5.28 0.86 3.36 12.87 5.10 -2.38 3.21 8.58 9.80 4.97 -4.98 4.34 11.95 17.31 8.16
Mitochondria -12.07 1.78 -2.25 11.20 -4.14 -11.24 5.28 3.57 12.43 -3.83 -16.08 5.55 -1.42 14.45 -4.32
Mitotic Spindle -10.42 -1.64 -29.66* -21.94* -19.46 -5.91 -2.07 -12.08 -11.11 -9.32 -10.72 -2.18 -32.02 -27.35 -21.36
None 1.84 5.04 3.05 2.73 -8.29 6.54 6.40 3.67 -1.18 -2.21 6.09 9.98 7.41 1.53 -5.04
Nuclear Periphery -4.49 -7.99 -16.93 -15.72 -4.95 -2.80 -8.22 -7.02 -6.39 -2.27 -2.78 -10.44 -17.10 -16.42 -7.12
Nuclear Periphery Foci -2.15 -2.49 -9.34 -7.76 -0.38 4.48 -3.05 -4.00 -4.68 1.15 2.51 -3.68 -8.94 -10.21 1.17
Nucleolus 3.32 4.81 1.83 -4.54 -7.30 4.04 1.91 -0.45 -2.33 -1.81 4.99 5.09 -0.63 -5.91 -5.63
Nucleus 11.66 -7.94 -1.88 -24.73 7.62 5.99 -11.42 -7.75 -9.55 4.86 12.24 -16.48 -15.91 -25.40 9.23
Peroxisomes -11.17 3.69 3.73 17.25 -0.40 -11.60 5.90 10.38 18.28 2.13 -16.88 8.55 11.49 24.89 1.31
Vacuole -10.38 -7.53 -12.95 -7.39 4.63 -13.57 -6.70 -6.39 -3.81 2.02 -16.16 -9.96 -13.84 -6.84 5.64
Vacuole Periphery -8.89 -1.62 -2.45 6.43 -1.75 -7.46 0.81 1.50 9.21 1.44 -10.32 0.40 -1.24 8.34 -1.84
Standard name
Human Ortholog
Description Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (21%), nucleus (9%), mixed (35%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (2.9e-06)
Subcompartmental Group N/A

Cik1

Cik1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cik1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available