Standard name
Human Ortholog
Description Peripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0.05 0 0 0.05 0.05
Bud 0.12 0.12 0.11 0.11 0.19 0.15 0.17 0.21 0.21 0.22 0.16 0.17 0.1 0.09 0.11 0.08 0.09 0.07 0.05 0.06 0 0.07 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0.05 0.06 0
Bud Site 0.17 0.18 0.1 0.12 0.08 0.12 0.12 0.07 0.13 0.11 0.09 0.09 0.12 0.05 0.05 0.07 0.06 0.12 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.07 0.07 0.1 0.09 0.1 0.07 0.08 0.09 0.11 0.09 0.05 0.13 0.12 0.18 0.08 0.08 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0.07 0.06 0 0 0 0 0 0 0 0 0.05 0 0.12 0.16 0.15 0.07 0.11 0.06 0.08 0.11 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.51 0.07 0.25 0.14 0.21 0.35 0.22 0.35 0.44 0.65 0.49 0 0 0 0.15 0.1 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.5 0.48 0.69 0.62 0.65 0.51 0.52 0.47 0.44 0.31 0.31 0.43 0.42 0.42 0.5 0.69 0.63 0.54 0.61 0.48 0.67 0.53 0.57 0.66
SpindlePole 0.31 0.19 0.48 0.4 0.36 0.43 0.41 0.41 0.37 0.39 0.18 0.28 0.52 0.53 0.41 0.4 0.29 0.38 0.12 0.13 0.13 0.16 0.08 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 11 9 5 10 4 10 5 11 7 3 7 15 24 32 2 3 3 5 7 5 3 24 31
Bud 23 13 18 15 44 36 58 74 60 72 39 48 29 52 53 15 13 5 10 8 5 7 17 16
Bud Neck 7 2 1 1 5 0 5 1 3 6 1 1 5 12 11 1 4 1 10 15 4 5 27 23
Bud Site 31 20 16 17 19 27 40 25 38 35 23 25 35 31 23 14 8 9 – – – – – –
Cell Periphery 1 2 1 0 0 0 1 0 2 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 25 8 12 13 21 23 25 28 27 36 23 15 37 68 86 16 11 10 0 1 1 0 10 4
Endoplasmic Reticulum 1 1 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1 2 0 0 1 0 1 1
Endosome 5 3 13 10 14 8 5 15 2 3 1 5 8 30 17 23 23 11 15 15 9 8 52 49
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 0 3 0 0 0 1 5
Mitochondria 51 56 12 34 33 49 119 78 100 143 162 134 9 10 4 29 15 6 4 3 5 4 21 23
Nucleus 1 0 0 0 0 1 2 1 1 1 1 1 2 3 0 2 1 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 4 3 1 3 2 2 4 5 5 4 0 3 1 1 1 2 0 1 0 0 0 0 2 1
Peroxisomes 92 53 113 84 153 119 177 169 127 100 77 118 119 239 244 132 90 40 123 65 115 56 278 397
SpindlePole 57 21 78 55 86 100 140 146 106 124 45 77 150 304 201 77 42 28 24 17 22 16 39 39
Vac/Vac Membrane 3 1 0 0 0 1 1 2 2 3 1 2 1 3 3 1 3 2 0 1 0 1 4 2
Unique Cell Count 184 110 163 136 237 233 343 358 287 322 251 275 286 571 490 191 143 74 203 138 173 108 490 604
Labelled Cell Count 302 194 274 237 388 370 588 550 484 534 376 440 411 778 677 316 216 119 203 138 173 108 490 604


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.8 4.6 4.2 4.6 3.9 3.8 3.8 3.6 3.5 3.1 3.2 5.8 5.8 5.7 6.3 7.0 6.5 4.9 5.7 5.9
Std Deviation (1e-4) 1.5 0.9 1.1 1.3 2.1 1.1 1.8 1.4 1.4 1.3 0.7 0.8 1.2 1.2 1.1 1.5 2.0 2.0 0.9 1.3 1.3
Intensity Change (Log2) – – – -0.16 -0.0 -0.24 -0.28 -0.27 -0.37 -0.42 -0.6 -0.52 0.33 0.33 0.31 0.43 0.6 0.49 0.09 0.3 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5896 2.0427 1.7043 1.3452 1.178 1.5767 -0.5395 0.2406 0.1634 0.0131 -0.0638 0.0229 -1.3072 -0.499 -0.6346 -0.7996 -0.5604 -0.8127
Actin 0.0136 0.0132 0.009 0.0055 0.0028 0.0077 0.0369 0.0247 0.0222 0.0022 0.0026 0.0215 0.022 0.0167 0.0113 0.023 0.0359 0.0142
Bud 0.0003 0.0045 0.0002 0.0001 0.0004 0.0013 0.0022 0.0044 0.0015 0.0028 0.0001 0.0002 0.0015 0.0034 0.0019 0.0013 0.0027 0.0006
Bud Neck 0.0025 0.0024 0.0508 0.0732 0.0473 0.0255 0.0056 0.0058 0.0313 0.057 0.0374 0.011 0.0111 0.0052 0.0572 0.0274 0.0068 0.0377
Bud Periphery 0.0003 0.007 0.0003 0.0001 0.0004 0.0018 0.0024 0.0044 0.0056 0.003 0.0002 0.0003 0.0009 0.0014 0.0015 0.0012 0.0031 0.0008
Bud Site 0.0221 0.0453 0.0175 0.0013 0.0014 0.004 0.019 0.0887 0.0214 0.0192 0.0004 0.0009 0.0164 0.0495 0.0327 0.0097 0.0107 0.0016
Cell Periphery 0.0001 0.0005 0.0003 0 0.0001 0.0002 0.0004 0.0002 0.0003 0.0004 0 0.0001 0.0002 0.0001 0.0002 0.0003 0.0002 0.0001
Cytoplasm 0.0006 0.0094 0.0024 0.0003 0.0001 0.0011 0.0083 0.0005 0.0011 0.0011 0.0002 0.001 0.0041 0.0018 0.0035 0.0009 0.0016 0.0062
Cytoplasmic Foci 0.5764 0.3935 0.5678 0.539 0.5922 0.6062 0.3824 0.3654 0.4407 0.4528 0.423 0.5419 0.5646 0.4903 0.4461 0.5673 0.5648 0.5619
Eisosomes 0.0003 0.0003 0.0001 0 0 0.0002 0.0002 0 0.0001 0 0 0.0001 0.0002 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0 0.0001 0.0003 0 0 0 0.0022 0 0.0005 0.0001 0.0003 0.0001 0.0017 0.0002 0.0002 0.0001 0.0007 0.0005
Endosome 0.0036 0.0185 0.0083 0.0034 0.0008 0.0062 0.0593 0.0088 0.0223 0.0145 0.0213 0.0171 0.0405 0.0307 0.0068 0.0299 0.0073 0.01
Golgi 0.0028 0.0077 0.0055 0.005 0.0026 0.0041 0.0243 0.0078 0.0115 0.0142 0.0169 0.0084 0.0127 0.0081 0.0058 0.0124 0.0116 0.0103
Lipid Particles 0.0412 0.0221 0.0336 0.0374 0.0286 0.0389 0.0584 0.0345 0.0241 0.0405 0.1299 0.0296 0.0353 0.0322 0.0263 0.0239 0.0508 0.045
Mitochondria 0.0011 0.0028 0.0027 0.0006 0.0005 0.0012 0.02 0.0053 0.0191 0.0172 0.0135 0.0074 0.0136 0.0044 0.0047 0.0026 0.0264 0.0098
None 0.0152 0.0152 0.0094 0.0002 0.0002 0.0163 0.0185 0.0003 0.0007 0.0154 0.0006 0.0009 0.0036 0.0014 0.0033 0.0004 0.0015 0.0014
Nuclear Periphery 0 0 0.0002 0.0001 0 0 0.0069 0 0.002 0.0001 0.0013 0 0.0035 0.0001 0.0038 0.0002 0.0007 0.0007
Nucleolus 0.0004 0.0004 0.0018 0.0003 0.0004 0.001 0.0032 0.0006 0.0005 0.0134 0.0011 0.0011 0.0005 0.0015 0.0013 0.0055 0.001 0.0008
Nucleus 0.0001 0.0003 0.0003 0.0001 0.0001 0.0004 0.0152 0.0001 0.0004 0.0171 0.0003 0.0001 0.0011 0.0004 0.0029 0.0008 0.0006 0.0005
Peroxisomes 0.2948 0.4403 0.2667 0.3085 0.27 0.2671 0.3191 0.4428 0.3721 0.2834 0.2894 0.3505 0.2483 0.3318 0.3801 0.2399 0.2586 0.2774
Punctate Nuclear 0.0246 0.0075 0.0219 0.0248 0.052 0.0159 0.0079 0.0052 0.0205 0.0423 0.0599 0.0066 0.0171 0.014 0.0096 0.0427 0.0139 0.0195
Vacuole 0.0001 0.0081 0.0008 0 0 0.0009 0.005 0.0003 0.0015 0.0025 0.0007 0.0009 0.0007 0.0065 0.0007 0.0092 0.0007 0.0006
Vacuole Periphery 0 0.0009 0.0001 0 0 0.0001 0.0024 0 0.0007 0.0008 0.0009 0.0002 0.0006 0.0003 0.0002 0.0013 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.4946 3.1239 1.159 6.1221 3.9645 5.2389 7.5016 5.0631 5.7031 7.0135
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1540 495 1333 1402 1496 1637 229 843 3036 2132 1562 2245

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.66 803.98 851.38 826.96 696.60 750.08 970.67 918.55 690.54 762.59 868.87 861.35
Standard Deviation 83.26 91.23 122.94 147.44 95.66 87.18 106.65 123.85 89.79 91.02 127.85 145.95
Intensity Change Log 2 — 0.231772 0.314415 0.272430 — 0.106714 0.478650 0.399028 — 0.170057 0.399578 0.337664

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009119 0.010500 0.030783 0.204034* 0.012123 0.009727 0.051095 0.243197* 0.010599 0.009906 0.033761 0.218740*
Bud Neck 0.022817 0.008634 0.012233 0.006788 0.013860 0.016864 0.007812 0.002610 0.018403 0.014954 0.011585 0.005219
Bud Site 0.051307 0.052082 0.111768* 0.158185* 0.044508 0.030400 0.116566* 0.155093* 0.047957 0.035434 0.112472* 0.157024*
Cell Periphery 0.001107 0.000287 0.000436 0.001452 0.000769 0.000640 0.000315 0.000957 0.000941 0.000558 0.000418 0.001267
Cytoplasm 0.018625 0.016356 0.023165 0.030755 0.017673 0.012154 0.011278 0.027917 0.018156 0.013129 0.021422 0.029689
Cytoplasmic Foci 0.474345* 0.243160* 0.282461* 0.194079* 0.527017* 0.369299* 0.299524* 0.189301* 0.500299* 0.340013* 0.284962* 0.192285*
Eisosomes 0.000374 0.000128 0.000280 0.001640 0.000255 0.000242 0.000328 0.001095 0.000316 0.000216 0.000287 0.001435
Endoplasmic Reticulum 0.000199 0.000219 0.000390 0.000682 0.000093 0.000100 0.000554 0.000478 0.000147 0.000128 0.000414 0.000605
Endosome 0.009587 0.019959 0.003987 0.003773 0.008883 0.013321 0.002343 0.003183 0.009240 0.014862 0.003746 0.003552
Golgi 0.014736 0.025405 0.005503 0.005474 0.013995 0.019455 0.002714 0.002801 0.014371 0.020837 0.005094 0.004470
Lipid Particles 0.015927 0.010787 0.006215 0.002399 0.009004 0.012742 0.005778 0.001052 0.012515 0.012288 0.006151 0.001893
Mitochondria 0.003636 0.009290 0.001204 0.003412 0.003282 0.007680 0.000294 0.002929 0.003461 0.008054 0.001070 0.003231
Mitotic Spindle 0.002116 0.014017 0.023364 0.006167 0.002494 0.003106 0.010691 0.007418 0.002302 0.005639 0.021506 0.006637
None 0.000710 0.000315 0.000260 0.000432 0.000518 0.000293 0.000085 0.000135 0.000616 0.000298 0.000234 0.000320
Nuclear Periphery 0.000037 0.000044 0.000056 0.000033 0.000009 0.000020 0.000038 0.000018 0.000023 0.000026 0.000053 0.000028
Nuclear Periphery Foci 0.000617 0.000256 0.000874 0.000308 0.000306 0.000468 0.000339 0.000146 0.000464 0.000419 0.000796 0.000247
Nucleolus 0.000382 0.000182 0.000275 0.000067 0.000191 0.000381 0.001114 0.000021 0.000288 0.000335 0.000398 0.000050
Nucleus 0.001442 0.001874 0.003781 0.002248 0.001171 0.001639 0.006107 0.001521 0.001309 0.001694 0.004122 0.001975
Peroxisomes 0.371988* 0.583158* 0.489015* 0.374237* 0.342835* 0.499857* 0.479385* 0.354285* 0.357623* 0.519197* 0.487603* 0.366745*
Vacuole 0.000832 0.002543 0.003792 0.003494 0.000711 0.001326 0.003598 0.005529 0.000772 0.001608 0.003763 0.004258
Vacuole Periphery 0.000098 0.000804 0.000159 0.000340 0.000303 0.000285 0.000046 0.000311 0.000199 0.000406 0.000142 0.000329

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.80 -6.78 -25.54* -24.86* -21.32* 1.30 -3.95 -21.26 -21.61* -13.17 0.48 -7.49 -33.09* -33.23* -26.87*
Bud Neck 4.92 4.01 6.69 0.89 3.53 -1.15 2.43 6.43 6.41 2.45 1.59 3.68 8.69 5.41 4.85
Bud Site -0.19 -9.20 -14.86 -11.79 -5.71 3.42 -5.20 -12.60 -14.51 -2.63 3.55 -11.46 -19.80 -21.94 -6.50
Cell Periphery 4.28 3.57 -1.28 -4.95 -4.37 0.64 3.09 -1.24 -1.87 -6.31 2.38 4.05 -1.89 -3.89 -5.46
Cytoplasm 0.66 -2.46 -5.15 -4.64 -2.63 2.59 1.13 -4.21 -6.38 -4.38 2.94 -2.47 -6.78 -9.49 -3.66
Cytoplasmic Foci 16.08 17.00 25.43 3.81 8.79 13.28 11.16 27.23 15.54 5.24 17.51 23.49 37.59 17.50 10.64
Eisosomes 3.26 1.38 -4.97 -6.07 -5.49 0.22 -1.31 -3.47 -3.51 -3.15 1.93 0.65 -6.15 -6.71 -6.40
Endoplasmic Reticulum -0.25 -3.26 -4.14 -3.64 -1.80 -0.14 -2.00 -3.93 -3.87 0.23 0.43 -4.67 -5.80 -6.08 -1.48
Endosome -3.23 4.19 4.31 5.28 0.59 -2.72 4.61 4.00 6.42 -0.68 -3.94 5.81 5.90 8.58 0.57
Golgi -4.09 8.81 6.79 7.73 0.05 -3.35 11.86 10.98 11.92 -0.12 -4.79 12.51 10.89 12.50 0.88
Lipid Particles 1.61 4.04 6.39 3.05 3.61 -1.80 0.99 6.48 7.44 1.32 0.22 3.88 8.55 7.51 4.24
Mitochondria -2.41 3.76 0.27 2.48 -3.11 -3.28 5.14 0.29 2.79 -2.14 -4.03 4.78 0.34 3.95 -3.20
Mitotic Spindle -3.94 -8.74 -4.43 2.33 6.75 -0.81 -1.91 -3.12 -2.78 0.90 -3.78 -8.88 -5.34 -1.31 6.60
None 0.97 0.82 0.50 -0.57 -0.36 1.74 3.95 3.46 1.65 -2.81 1.72 1.12 1.11 -0.48 -0.06
Nuclear Periphery -0.21 -1.54 -0.13 0.11 1.56 -1.73 -2.06 -3.27 -1.43 0.76 -0.22 -2.23 -1.04 -1.00 1.73
Nuclear Periphery Foci 1.85 -2.22 1.75 -0.32 4.17 -0.88 -0.44 0.64 1.10 0.93 -0.09 -3.15 1.76 0.95 4.41
Nucleolus 1.35 -0.55 2.54 1.60 2.47 -1.21 -0.85 3.51 2.02 1.00 -0.59 -1.33 3.50 2.08 2.47
Nucleus -0.45 -3.29 -2.11 -1.29 1.17 -0.61 -1.74 -2.13 -1.54 1.19 -0.62 -3.83 -3.02 -2.08 1.66
Peroxisomes -12.41 -9.05 0.14 12.32 8.93 -13.44 -5.92 -0.23 11.02 5.46 -17.35 -12.25 -0.39 15.77 11.16
Vacuole -2.34 -4.48 -4.51 -2.74 -0.68 -1.67 -2.13 -4.90 -4.62 -1.79 -2.56 -5.03 -6.66 -5.83 -2.15
Vacuole Periphery -2.13 -0.74 -1.75 1.31 -1.09 0.07 1.08 -0.01 -0.11 -1.55 -1.28 0.38 -0.83 0.52 -1.42
Standard name
Human Ortholog
Description Peripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane
Localization
Cell Percentages peroxisome (71%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Inp1

Inp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Inp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available