ORF
Human Ortholog
Description Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.13 0 0.06 0.07 0.08 0.11 0 0.06 0.06
Bud 0 0 0 0 0 0 – 0 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.07 0.12 0 0 0.06 0 0 0.05
Cytoplasm 0.85 0.73 0.87 0.79 0.78 0.5 – 0.55 0.45 0.39 0.35 0.35 0.96 0.98 0.97 0.82 0.77 0.66 0.19 0.19 0.15 0.38 0.36 0.3
Endoplasmic Reticulum 0 0.21 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.31 0.37 0.27 0.17 0.2 0.24
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0.05 0.24 0.33 0.63 – 0.53 0.63 0.72 0.76 0.78 0 0 0 0 0.07 0.1 0.27 0.19 0.3 0.27 0.21 0.2
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 15 6 0 0 0 – 0 0 1 0 0 1 3 0 5 4 6 50 45 61 28 47 50
Bud 9 5 5 5 9 21 – 32 41 57 28 43 0 0 1 0 0 2 4 2 3 7 14 25
Bud Neck 7 1 5 0 3 0 – 2 2 3 0 0 4 12 3 0 1 1 15 12 11 10 14 13
Bud Site 1 1 0 0 0 4 – 1 5 20 6 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 9 7 7 3 – 4 8 5 7 6 7 16 11 3 8 12 16 9 32 16 25 39
Cytoplasm 556 327 375 269 302 270 – 407 323 338 209 251 491 722 770 32 90 67 148 108 81 264 305 253
Endoplasmic Reticulum 16 93 23 6 2 8 – 4 1 2 6 3 12 9 8 2 8 7 238 203 148 116 171 199
Endosome 2 5 0 3 8 0 – 6 3 0 3 1 3 0 2 0 0 4 18 14 16 9 17 17
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 1 1 1 0 1 1 2 7 3 5 4 7
Mitochondria 99 18 23 83 130 336 – 397 456 621 450 556 0 0 0 1 8 10 205 108 167 187 183 170
Nucleus 0 1 1 3 1 2 – 12 5 4 0 2 1 1 2 1 1 2 0 0 1 1 1 0
Nuclear Periphery 1 1 0 1 0 2 – 1 4 0 1 4 0 0 0 0 0 0 2 0 0 2 1 2
Nucleolus 0 0 0 0 0 3 – 0 0 1 3 0 0 0 0 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 2 2 3 0 0 1
SpindlePole 2 0 6 2 2 5 – 12 16 18 10 12 3 6 3 0 1 3 6 3 2 4 11 11
Vac/Vac Membrane 12 6 5 7 7 17 – 15 23 27 26 31 1 6 14 0 6 7 32 28 10 30 41 37
Unique Cell Count 653 446 432 342 389 536 743 721 863 596 717 512 738 794 39 117 102 760 556 556 699 857 845
Labelled Cell Count 710 480 458 386 471 671 893 887 1097 749 920 525 776 815 44 128 122 760 556 556 699 857 845


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.8 4.3 4.0 4.1 3.5 – 3.6 3.3 3.1 3.0 3.1 5.8 5.9 5.8 6.3 6.3 6.0 5.9 6.0 6.5
Std Deviation (1e-4) 0.4 0.7 0.9 1.0 1.4 1.4 – 1.4 1.0 0.7 0.8 0.9 1.0 1.1 1.3 0.9 1.5 1.7 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.11 -0.06 -0.27 – -0.26 -0.36 -0.45 -0.52 -0.47 0.44 0.45 0.43 0.57 0.55 0.49 0.47 0.48 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3392 4.5233 4.6829 3.7556 4.5005 4.5365 2.2576 2.2377 1.9983 2.6818 2.6887 1.9999 1.1737 1.5408 1.2363 1.2317 0.9663 1.1152
Actin 0.0149 0.003 0.0104 0.0025 0.0157 0.0029 0.0189 0.0072 0.0093 0.0404 0.0213 0.004 0.0236 0.0124 0.0267 0.0052 0.0066 0.003
Bud 0.0005 0.0009 0.0046 0.0001 0.0002 0.0018 0.0016 0.0038 0.0011 0.0007 0.0016 0.0006 0.0003 0.0005 0.0008 0.0004 0.0008 0.0021
Bud Neck 0.0026 0.001 0.0038 0.002 0.0012 0.0103 0.0074 0.0017 0.0049 0.0106 0.0081 0.0075 0.0024 0.0014 0.001 0.0013 0.0142 0.0104
Bud Periphery 0.001 0.0012 0.0038 0.0004 0.0003 0.001 0.0054 0.0032 0.0028 0.0013 0.004 0.0013 0.0005 0.0006 0.0018 0.0008 0.002 0.0011
Bud Site 0.0072 0.0241 0.0114 0.0005 0.0006 0.0011 0.0076 0.0435 0.0078 0.0065 0.012 0.0011 0.006 0.0261 0.0045 0.0033 0.0032 0.0003
Cell Periphery 0.0043 0.0035 0.0036 0.0038 0.0013 0.0012 0.0022 0.0021 0.0017 0.001 0.0011 0.0007 0.0022 0.0022 0.0017 0.0014 0.0012 0.0009
Cytoplasm 0.2465 0.2824 0.3096 0.3912 0.2924 0.3618 0.3221 0.4732 0.4672 0.3933 0.4756 0.5204 0.38 0.4615 0.4367 0.4992 0.5092 0.4441
Cytoplasmic Foci 0.0274 0.0158 0.0312 0.0491 0.017 0.0369 0.0337 0.0221 0.0297 0.0382 0.0288 0.0311 0.0235 0.0224 0.0162 0.0279 0.0308 0.0328
Eisosomes 0.0011 0.0009 0.0009 0.0003 0.0027 0.0003 0.0004 0.0004 0.0002 0.0008 0.0004 0.0001 0.0006 0.0003 0.0006 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.108 0.0553 0.0695 0.1795 0.1172 0.0895 0.2155 0.1339 0.1348 0.1689 0.0907 0.1207 0.1647 0.1237 0.1698 0.1341 0.1107 0.1203
Endosome 0.0237 0.0171 0.0258 0.0134 0.0245 0.0238 0.0378 0.0283 0.0398 0.0345 0.051 0.031 0.0265 0.025 0.0215 0.0226 0.021 0.0304
Golgi 0.005 0.0021 0.0035 0.0023 0.004 0.0023 0.0057 0.0045 0.0055 0.0084 0.0103 0.0033 0.0039 0.0031 0.0049 0.0028 0.0033 0.0024
Lipid Particles 0.0249 0.0048 0.0064 0.0088 0.009 0.0133 0.0198 0.0078 0.0094 0.0176 0.0222 0.0094 0.014 0.0052 0.0068 0.008 0.0078 0.0109
Mitochondria 0.0043 0.0026 0.005 0.0009 0.002 0.0032 0.003 0.0028 0.0048 0.0406 0.0082 0.0058 0.0012 0.0007 0.0018 0.0008 0.0009 0.0024
None 0.4833 0.5657 0.4849 0.2856 0.4437 0.4284 0.2489 0.2246 0.2258 0.173 0.2001 0.2221 0.318 0.2949 0.2879 0.2623 0.2208 0.3157
Nuclear Periphery 0.0111 0.0045 0.0069 0.0089 0.0091 0.0042 0.018 0.0117 0.017 0.0082 0.0223 0.0139 0.0114 0.0065 0.0062 0.0068 0.0119 0.0081
Nucleolus 0.0019 0.0011 0.0012 0.0008 0.0014 0.0012 0.0029 0.0019 0.0018 0.0048 0.0038 0.0008 0.0007 0.0004 0.0006 0.0004 0.0024 0.0004
Nucleus 0.0039 0.005 0.0036 0.004 0.0045 0.005 0.0097 0.0093 0.0111 0.0078 0.0113 0.0076 0.0039 0.0039 0.0028 0.0047 0.0188 0.005
Peroxisomes 0.0114 0.0007 0.0021 0.0017 0.0019 0.0012 0.0065 0.001 0.0027 0.0237 0.006 0.0017 0.0065 0.0004 0.0014 0.0007 0.0213 0.0007
Punctate Nuclear 0.01 0.0032 0.0048 0.0386 0.0464 0.006 0.0185 0.0091 0.0108 0.0125 0.0148 0.012 0.0049 0.0038 0.0018 0.0052 0.008 0.0039
Vacuole 0.0058 0.0044 0.0057 0.0047 0.0042 0.004 0.0123 0.007 0.0098 0.0064 0.0047 0.004 0.0043 0.0044 0.004 0.0113 0.0045 0.0041
Vacuole Periphery 0.0013 0.0005 0.0012 0.0007 0.0006 0.0006 0.0021 0.0009 0.0016 0.0011 0.0018 0.0009 0.0006 0.0005 0.0005 0.0006 0.0005 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.6903 18.6651 14.9157 23.5202 23.9477 22.4732 21.2312 17.6365 18.1565 23.3113
Translational Efficiency 0.6704 0.7142 0.8972 0.5552 0.5384 0.5948 0.6004 0.6841 0.7449 0.6831

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘