ORF | |
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Human Ortholog | |
Description | Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 15 | 6 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 5 | 4 | 6 | 50 | 45 | 61 | 28 | 47 | 50 |
Bud | 9 | 5 | 5 | 5 | 9 | 21 | – | 32 | 41 | 57 | 28 | 43 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 2 | 3 | 7 | 14 | 25 |
Bud Neck | 7 | 1 | 5 | 0 | 3 | 0 | – | 2 | 2 | 3 | 0 | 0 | 4 | 12 | 3 | 0 | 1 | 1 | 15 | 12 | 11 | 10 | 14 | 13 |
Bud Site | 1 | 1 | 0 | 0 | 0 | 4 | – | 1 | 5 | 20 | 6 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 6 | 9 | 7 | 7 | 3 | – | 4 | 8 | 5 | 7 | 6 | 7 | 16 | 11 | 3 | 8 | 12 | 16 | 9 | 32 | 16 | 25 | 39 |
Cytoplasm | 556 | 327 | 375 | 269 | 302 | 270 | – | 407 | 323 | 338 | 209 | 251 | 491 | 722 | 770 | 32 | 90 | 67 | 148 | 108 | 81 | 264 | 305 | 253 |
Endoplasmic Reticulum | 16 | 93 | 23 | 6 | 2 | 8 | – | 4 | 1 | 2 | 6 | 3 | 12 | 9 | 8 | 2 | 8 | 7 | 238 | 203 | 148 | 116 | 171 | 199 |
Endosome | 2 | 5 | 0 | 3 | 8 | 0 | – | 6 | 3 | 0 | 3 | 1 | 3 | 0 | 2 | 0 | 0 | 4 | 18 | 14 | 16 | 9 | 17 | 17 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 7 | 3 | 5 | 4 | 7 |
Mitochondria | 99 | 18 | 23 | 83 | 130 | 336 | – | 397 | 456 | 621 | 450 | 556 | 0 | 0 | 0 | 1 | 8 | 10 | 205 | 108 | 167 | 187 | 183 | 170 |
Nucleus | 0 | 1 | 1 | 3 | 1 | 2 | – | 12 | 5 | 4 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 2 | – | 1 | 4 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 6 | 2 | 2 | 5 | – | 12 | 16 | 18 | 10 | 12 | 3 | 6 | 3 | 0 | 1 | 3 | 6 | 3 | 2 | 4 | 11 | 11 |
Vac/Vac Membrane | 12 | 6 | 5 | 7 | 7 | 17 | – | 15 | 23 | 27 | 26 | 31 | 1 | 6 | 14 | 0 | 6 | 7 | 32 | 28 | 10 | 30 | 41 | 37 |
Unique Cell Count | 653 | 446 | 432 | 342 | 389 | 536 | 743 | 721 | 863 | 596 | 717 | 512 | 738 | 794 | 39 | 117 | 102 | 760 | 556 | 556 | 699 | 857 | 845 | |
Labelled Cell Count | 710 | 480 | 458 | 386 | 471 | 671 | 893 | 887 | 1097 | 749 | 920 | 525 | 776 | 815 | 44 | 128 | 122 | 760 | 556 | 556 | 699 | 857 | 845 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.8 | 4.3 | 4.0 | 4.1 | 3.5 | – | 3.6 | 3.3 | 3.1 | 3.0 | 3.1 | 5.8 | 5.9 | 5.8 | 6.3 | 6.3 | 6.0 | 5.9 | 6.0 | 6.5 |
Std Deviation (1e-4) | 0.4 | 0.7 | 0.9 | 1.0 | 1.4 | 1.4 | – | 1.4 | 1.0 | 0.7 | 0.8 | 0.9 | 1.0 | 1.1 | 1.3 | 0.9 | 1.5 | 1.7 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.06 | -0.27 | – | -0.26 | -0.36 | -0.45 | -0.52 | -0.47 | 0.44 | 0.45 | 0.43 | 0.57 | 0.55 | 0.49 | 0.47 | 0.48 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3392 | 4.5233 | 4.6829 | 3.7556 | 4.5005 | 4.5365 | 2.2576 | 2.2377 | 1.9983 | 2.6818 | 2.6887 | 1.9999 | 1.1737 | 1.5408 | 1.2363 | 1.2317 | 0.9663 | 1.1152 |
Actin | 0.0149 | 0.003 | 0.0104 | 0.0025 | 0.0157 | 0.0029 | 0.0189 | 0.0072 | 0.0093 | 0.0404 | 0.0213 | 0.004 | 0.0236 | 0.0124 | 0.0267 | 0.0052 | 0.0066 | 0.003 |
Bud | 0.0005 | 0.0009 | 0.0046 | 0.0001 | 0.0002 | 0.0018 | 0.0016 | 0.0038 | 0.0011 | 0.0007 | 0.0016 | 0.0006 | 0.0003 | 0.0005 | 0.0008 | 0.0004 | 0.0008 | 0.0021 |
Bud Neck | 0.0026 | 0.001 | 0.0038 | 0.002 | 0.0012 | 0.0103 | 0.0074 | 0.0017 | 0.0049 | 0.0106 | 0.0081 | 0.0075 | 0.0024 | 0.0014 | 0.001 | 0.0013 | 0.0142 | 0.0104 |
Bud Periphery | 0.001 | 0.0012 | 0.0038 | 0.0004 | 0.0003 | 0.001 | 0.0054 | 0.0032 | 0.0028 | 0.0013 | 0.004 | 0.0013 | 0.0005 | 0.0006 | 0.0018 | 0.0008 | 0.002 | 0.0011 |
Bud Site | 0.0072 | 0.0241 | 0.0114 | 0.0005 | 0.0006 | 0.0011 | 0.0076 | 0.0435 | 0.0078 | 0.0065 | 0.012 | 0.0011 | 0.006 | 0.0261 | 0.0045 | 0.0033 | 0.0032 | 0.0003 |
Cell Periphery | 0.0043 | 0.0035 | 0.0036 | 0.0038 | 0.0013 | 0.0012 | 0.0022 | 0.0021 | 0.0017 | 0.001 | 0.0011 | 0.0007 | 0.0022 | 0.0022 | 0.0017 | 0.0014 | 0.0012 | 0.0009 |
Cytoplasm | 0.2465 | 0.2824 | 0.3096 | 0.3912 | 0.2924 | 0.3618 | 0.3221 | 0.4732 | 0.4672 | 0.3933 | 0.4756 | 0.5204 | 0.38 | 0.4615 | 0.4367 | 0.4992 | 0.5092 | 0.4441 |
Cytoplasmic Foci | 0.0274 | 0.0158 | 0.0312 | 0.0491 | 0.017 | 0.0369 | 0.0337 | 0.0221 | 0.0297 | 0.0382 | 0.0288 | 0.0311 | 0.0235 | 0.0224 | 0.0162 | 0.0279 | 0.0308 | 0.0328 |
Eisosomes | 0.0011 | 0.0009 | 0.0009 | 0.0003 | 0.0027 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.108 | 0.0553 | 0.0695 | 0.1795 | 0.1172 | 0.0895 | 0.2155 | 0.1339 | 0.1348 | 0.1689 | 0.0907 | 0.1207 | 0.1647 | 0.1237 | 0.1698 | 0.1341 | 0.1107 | 0.1203 |
Endosome | 0.0237 | 0.0171 | 0.0258 | 0.0134 | 0.0245 | 0.0238 | 0.0378 | 0.0283 | 0.0398 | 0.0345 | 0.051 | 0.031 | 0.0265 | 0.025 | 0.0215 | 0.0226 | 0.021 | 0.0304 |
Golgi | 0.005 | 0.0021 | 0.0035 | 0.0023 | 0.004 | 0.0023 | 0.0057 | 0.0045 | 0.0055 | 0.0084 | 0.0103 | 0.0033 | 0.0039 | 0.0031 | 0.0049 | 0.0028 | 0.0033 | 0.0024 |
Lipid Particles | 0.0249 | 0.0048 | 0.0064 | 0.0088 | 0.009 | 0.0133 | 0.0198 | 0.0078 | 0.0094 | 0.0176 | 0.0222 | 0.0094 | 0.014 | 0.0052 | 0.0068 | 0.008 | 0.0078 | 0.0109 |
Mitochondria | 0.0043 | 0.0026 | 0.005 | 0.0009 | 0.002 | 0.0032 | 0.003 | 0.0028 | 0.0048 | 0.0406 | 0.0082 | 0.0058 | 0.0012 | 0.0007 | 0.0018 | 0.0008 | 0.0009 | 0.0024 |
None | 0.4833 | 0.5657 | 0.4849 | 0.2856 | 0.4437 | 0.4284 | 0.2489 | 0.2246 | 0.2258 | 0.173 | 0.2001 | 0.2221 | 0.318 | 0.2949 | 0.2879 | 0.2623 | 0.2208 | 0.3157 |
Nuclear Periphery | 0.0111 | 0.0045 | 0.0069 | 0.0089 | 0.0091 | 0.0042 | 0.018 | 0.0117 | 0.017 | 0.0082 | 0.0223 | 0.0139 | 0.0114 | 0.0065 | 0.0062 | 0.0068 | 0.0119 | 0.0081 |
Nucleolus | 0.0019 | 0.0011 | 0.0012 | 0.0008 | 0.0014 | 0.0012 | 0.0029 | 0.0019 | 0.0018 | 0.0048 | 0.0038 | 0.0008 | 0.0007 | 0.0004 | 0.0006 | 0.0004 | 0.0024 | 0.0004 |
Nucleus | 0.0039 | 0.005 | 0.0036 | 0.004 | 0.0045 | 0.005 | 0.0097 | 0.0093 | 0.0111 | 0.0078 | 0.0113 | 0.0076 | 0.0039 | 0.0039 | 0.0028 | 0.0047 | 0.0188 | 0.005 |
Peroxisomes | 0.0114 | 0.0007 | 0.0021 | 0.0017 | 0.0019 | 0.0012 | 0.0065 | 0.001 | 0.0027 | 0.0237 | 0.006 | 0.0017 | 0.0065 | 0.0004 | 0.0014 | 0.0007 | 0.0213 | 0.0007 |
Punctate Nuclear | 0.01 | 0.0032 | 0.0048 | 0.0386 | 0.0464 | 0.006 | 0.0185 | 0.0091 | 0.0108 | 0.0125 | 0.0148 | 0.012 | 0.0049 | 0.0038 | 0.0018 | 0.0052 | 0.008 | 0.0039 |
Vacuole | 0.0058 | 0.0044 | 0.0057 | 0.0047 | 0.0042 | 0.004 | 0.0123 | 0.007 | 0.0098 | 0.0064 | 0.0047 | 0.004 | 0.0043 | 0.0044 | 0.004 | 0.0113 | 0.0045 | 0.0041 |
Vacuole Periphery | 0.0013 | 0.0005 | 0.0012 | 0.0007 | 0.0006 | 0.0006 | 0.0021 | 0.0009 | 0.0016 | 0.0011 | 0.0018 | 0.0009 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.6903 | 18.6651 | 14.9157 | 23.5202 | 23.9477 | 22.4732 | 21.2312 | 17.6365 | 18.1565 | 23.3113 |
Translational Efficiency | 0.6704 | 0.7142 | 0.8972 | 0.5552 | 0.5384 | 0.5948 | 0.6004 | 0.6841 | 0.7449 | 0.6831 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |