ORF
Description Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.13 0 0.06 0.07 0.08 0.11 0 0.06 0.06
Bud 0 0 0 0 0 0 – 0 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.07 0.12 0 0 0.06 0 0 0.05
Cytoplasm 0.85 0.73 0.87 0.79 0.78 0.5 – 0.55 0.45 0.39 0.35 0.35 0.96 0.98 0.97 0.82 0.77 0.66 0.19 0.19 0.15 0.38 0.36 0.3
Endoplasmic Reticulum 0 0.21 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.31 0.37 0.27 0.17 0.2 0.24
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0.05 0.24 0.33 0.63 – 0.53 0.63 0.72 0.76 0.78 0 0 0 0 0.07 0.1 0.27 0.19 0.3 0.27 0.21 0.2
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 15 6 0 0 0 – 0 0 1 0 0 1 3 0 5 4 6 50 45 61 28 47 50
Bud 9 5 5 5 9 21 – 32 41 57 28 43 0 0 1 0 0 2 4 2 3 7 14 25
Bud Neck 7 1 5 0 3 0 – 2 2 3 0 0 4 12 3 0 1 1 15 12 11 10 14 13
Bud Site 1 1 0 0 0 4 – 1 5 20 6 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 9 7 7 3 – 4 8 5 7 6 7 16 11 3 8 12 16 9 32 16 25 39
Cytoplasm 556 327 375 269 302 270 – 407 323 338 209 251 491 722 770 32 90 67 148 108 81 264 305 253
Endoplasmic Reticulum 16 93 23 6 2 8 – 4 1 2 6 3 12 9 8 2 8 7 238 203 148 116 171 199
Endosome 2 5 0 3 8 0 – 6 3 0 3 1 3 0 2 0 0 4 18 14 16 9 17 17
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 1 1 1 0 1 1 2 7 3 5 4 7
Mitochondria 99 18 23 83 130 336 – 397 456 621 450 556 0 0 0 1 8 10 205 108 167 187 183 170
Nucleus 0 1 1 3 1 2 – 12 5 4 0 2 1 1 2 1 1 2 0 0 1 1 1 0
Nuclear Periphery 1 1 0 1 0 2 – 1 4 0 1 4 0 0 0 0 0 0 2 0 0 2 1 2
Nucleolus 0 0 0 0 0 3 – 0 0 1 3 0 0 0 0 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 2 2 3 0 0 1
SpindlePole 2 0 6 2 2 5 – 12 16 18 10 12 3 6 3 0 1 3 6 3 2 4 11 11
Vac/Vac Membrane 12 6 5 7 7 17 – 15 23 27 26 31 1 6 14 0 6 7 32 28 10 30 41 37
Unique Cell Count 653 446 432 342 389 536 743 721 863 596 717 512 738 794 39 117 102 760 556 556 699 857 845
Labelled Cell Count 710 480 458 386 471 671 893 887 1097 749 920 525 776 815 44 128 122 760 556 556 699 857 845


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.8 4.3 4.0 4.1 3.5 – 3.6 3.3 3.1 3.0 3.1 5.8 5.9 5.8 6.3 6.3 6.0 5.9 6.0 6.5
Std Deviation (1e-4) 0.4 0.7 0.9 1.0 1.4 1.4 – 1.4 1.0 0.7 0.8 0.9 1.0 1.1 1.3 0.9 1.5 1.7 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.11 -0.06 -0.27 – -0.26 -0.36 -0.45 -0.52 -0.47 0.44 0.45 0.43 0.57 0.55 0.49 0.47 0.48 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –