ORF | |
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Description | Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 15 | 6 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 5 | 4 | 6 | 50 | 45 | 61 | 28 | 47 | 50 |
Bud | 9 | 5 | 5 | 5 | 9 | 21 | – | 32 | 41 | 57 | 28 | 43 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 2 | 3 | 7 | 14 | 25 |
Bud Neck | 7 | 1 | 5 | 0 | 3 | 0 | – | 2 | 2 | 3 | 0 | 0 | 4 | 12 | 3 | 0 | 1 | 1 | 15 | 12 | 11 | 10 | 14 | 13 |
Bud Site | 1 | 1 | 0 | 0 | 0 | 4 | – | 1 | 5 | 20 | 6 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 6 | 9 | 7 | 7 | 3 | – | 4 | 8 | 5 | 7 | 6 | 7 | 16 | 11 | 3 | 8 | 12 | 16 | 9 | 32 | 16 | 25 | 39 |
Cytoplasm | 556 | 327 | 375 | 269 | 302 | 270 | – | 407 | 323 | 338 | 209 | 251 | 491 | 722 | 770 | 32 | 90 | 67 | 148 | 108 | 81 | 264 | 305 | 253 |
Endoplasmic Reticulum | 16 | 93 | 23 | 6 | 2 | 8 | – | 4 | 1 | 2 | 6 | 3 | 12 | 9 | 8 | 2 | 8 | 7 | 238 | 203 | 148 | 116 | 171 | 199 |
Endosome | 2 | 5 | 0 | 3 | 8 | 0 | – | 6 | 3 | 0 | 3 | 1 | 3 | 0 | 2 | 0 | 0 | 4 | 18 | 14 | 16 | 9 | 17 | 17 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 7 | 3 | 5 | 4 | 7 |
Mitochondria | 99 | 18 | 23 | 83 | 130 | 336 | – | 397 | 456 | 621 | 450 | 556 | 0 | 0 | 0 | 1 | 8 | 10 | 205 | 108 | 167 | 187 | 183 | 170 |
Nucleus | 0 | 1 | 1 | 3 | 1 | 2 | – | 12 | 5 | 4 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 2 | – | 1 | 4 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 6 | 2 | 2 | 5 | – | 12 | 16 | 18 | 10 | 12 | 3 | 6 | 3 | 0 | 1 | 3 | 6 | 3 | 2 | 4 | 11 | 11 |
Vac/Vac Membrane | 12 | 6 | 5 | 7 | 7 | 17 | – | 15 | 23 | 27 | 26 | 31 | 1 | 6 | 14 | 0 | 6 | 7 | 32 | 28 | 10 | 30 | 41 | 37 |
Unique Cell Count | 653 | 446 | 432 | 342 | 389 | 536 | 743 | 721 | 863 | 596 | 717 | 512 | 738 | 794 | 39 | 117 | 102 | 760 | 556 | 556 | 699 | 857 | 845 | |
Labelled Cell Count | 710 | 480 | 458 | 386 | 471 | 671 | 893 | 887 | 1097 | 749 | 920 | 525 | 776 | 815 | 44 | 128 | 122 | 760 | 556 | 556 | 699 | 857 | 845 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.8 | 4.3 | 4.0 | 4.1 | 3.5 | – | 3.6 | 3.3 | 3.1 | 3.0 | 3.1 | 5.8 | 5.9 | 5.8 | 6.3 | 6.3 | 6.0 | 5.9 | 6.0 | 6.5 |
Std Deviation (1e-4) | 0.4 | 0.7 | 0.9 | 1.0 | 1.4 | 1.4 | – | 1.4 | 1.0 | 0.7 | 0.8 | 0.9 | 1.0 | 1.1 | 1.3 | 0.9 | 1.5 | 1.7 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.06 | -0.27 | – | -0.26 | -0.36 | -0.45 | -0.52 | -0.47 | 0.44 | 0.45 | 0.43 | 0.57 | 0.55 | 0.49 | 0.47 | 0.48 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |