Standard name
Human Ortholog
Description Phosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – 0.97 0.9 0.9 0.86 0.74 0.66 0.66 0.66 0.6 0.6 0.96 – 0.96 0.97 0.97 0.96 0.71 0.67 0.89 0.79 0.67 0.82
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0.12 0 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.24 0 0 0 0
Mitochondria – – 0 0.1 0 0.13 0.2 0.21 0.23 0.31 0.41 0.4 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus – – 0 0 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 0.05 0.05 0 0.06 0.09 0.18 0.16 0.12 0.12 0.13 0 – 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0
Bud – – 0 0 0 0 0 1 1 0 4 1 0 – 0 0 0 0 0 0 2 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 – – – – – –
Cell Periphery – – 1 0 1 4 4 3 5 5 5 5 0 – 0 3 4 7 0 0 0 0 0 0
Cytoplasm – – 114 53 89 185 188 109 144 126 92 99 22 – 52 215 244 237 11 4 112 30 44 49
Endoplasmic Reticulum – – 0 0 2 2 5 1 5 3 3 2 1 – 0 10 8 13 1 0 2 0 1 1
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 2 0 0 1 0 4 2
Golgi – – 0 0 0 0 1 0 0 0 0 0 0 – 0 1 0 2 0 1 0 0 1 0
Mitochondria – – 3 6 4 27 50 34 49 59 63 66 0 – 1 0 1 2 0 0 0 1 0 0
Nucleus – – 0 0 0 5 4 8 5 1 2 1 0 – 0 1 3 1 0 0 0 3 1 1
Nuclear Periphery – – 0 0 0 0 2 0 2 1 3 0 0 – 0 0 1 0 0 0 0 0 2 0
Nucleolus – – 0 1 0 1 1 0 0 0 0 0 0 – 1 0 1 0 0 0 0 0 0 1
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 6 3 4 12 23 30 34 22 18 21 0 – 0 5 9 10 0 0 1 0 1 0
Unique Cell Count 118 59 99 215 255 165 217 190 154 166 23 54 222 252 248 16 6 127 38 67 60
Labelled Cell Count 124 63 100 236 278 186 245 217 190 195 23 54 235 272 274 16 6 127 38 67 60


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 9.7 8.7 9.1 8.2 8.0 7.4 7.3 7.3 6.8 6.4 11.8 – 10.3 13.5 14.0 14.7 – 10.6 12.0
Std Deviation (1e-4) – – 2.4 2.2 2.2 1.9 2.0 1.5 1.6 2.0 1.7 1.7 2.1 – 3.0 3.2 3.3 3.8 – 2.3 2.0
Intensity Change (Log2) – – – -0.15 -0.1 -0.24 -0.27 -0.38 -0.41 -0.41 -0.51 -0.6 0.29 – 0.08 0.49 0.54 0.61 – 0.13 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Cytoplasm -1.8 -2.0 -3.0 -5.2 -6.2 -6.3 -6.2 -7.0 -7.1 -0.2 – -0.1 0.1 0.1 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Mitochondria 0 0 3.1 4.4 4.4 4.8 6.1 7.3 7.2 0 – 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Vacuole 0 0 0.2 1.3 3.3 2.9 1.9 1.9 2.1 0 – 0 0 0 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0225 0.0003 0.0035 0.0009 0.0004 0.0026 0.1438 0.0178 0.0068 0.0003 0.0108 0.0186 0.0361 0.0029 0.0367 0.0002 0.0001 0.0004
Bud 0.002 0.0001 0.0006 0.0001 0.0002 0.0001 0.0022 0.0005 0.001 0.0001 0.0002 0.0029 0.0193 0.0191 0.0016 0 0.0001 0.0001
Bud Neck 0.0014 0.0001 0.0003 0.0005 0.0007 0.0006 0.0068 0.0005 0.0011 0.0003 0.001 0.001 0.0095 0.0002 0.0004 0.0004 0.0006 0.0007
Bud Periphery 0.0014 0 0.0005 0 0.0001 0.0002 0.0033 0.0004 0.0008 0.0001 0.0002 0.0076 0.0283 0.0095 0.0017 0 0 0
Bud Site 0.0047 0.0005 0.0005 0.0002 0.0002 0.0005 0.0079 0.0075 0.0116 0.0001 0.0002 0.0063 0.0092 0.0225 0.0026 0 0 0.0001
Cell Periphery 0.0003 0 0.0001 0.0001 0.0001 0 0.001 0.0002 0.0003 0.0001 0.0001 0.0003 0.0016 0.0003 0.0001 0 0 0
Cytoplasm 0.0813 0.7874 0.731 0.6693 0.9791 0.8966 0.4194 0.8989 0.8101 0.9839 0.5102 0.7028 0.5392 0.8851 0.7751 0.9952 0.9693 0.8933
Cytoplasmic Foci 0.0543 0.02 0.0039 0.0075 0.0043 0.0047 0.099 0.0116 0.0464 0.0016 0.0027 0.0203 0.0991 0.0065 0.0139 0.0006 0.0012 0.0095
Eisosomes 0.0003 0 0.0001 0 0 0.0002 0.0014 0.0001 0.0001 0 0 0.0003 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0135 0.0024 0.0026 0.0021 0.0023 0.0019 0.012 0.0024 0.0019 0.0035 0.0347 0.0133 0.017 0.0038 0.0018 0.0002 0.002 0.002
Endosome 0.2502 0.1644 0.001 0.0022 0.0022 0.003 0.0289 0.0057 0.0331 0.0008 0.0454 0.08 0.0913 0.0072 0.0543 0.0001 0.0011 0.0428
Golgi 0.0829 0.0075 0.0002 0.0002 0.0001 0.0014 0.0146 0.003 0.025 0 0.0015 0.0151 0.0177 0.0006 0.0191 0 0 0.0366
Lipid Particles 0.017 0.0006 0.0001 0.0001 0 0.0066 0.0136 0.0025 0.0068 0 0.0005 0.0101 0.0164 0.0005 0.0059 0 0 0.0007
Mitochondria 0.0222 0.0004 0.0003 0.0002 0.0001 0.0144 0.0325 0.0008 0.0086 0.0001 0.0095 0.0428 0.0071 0.0005 0.0174 0.0001 0.0002 0.002
None 0.2738 0.0012 0.2496 0.3113 0.0054 0.0474 0.0227 0.0368 0.0138 0.003 0.003 0.0151 0.028 0.0336 0.052 0.0004 0.0023 0.0022
Nuclear Periphery 0.0682 0.0014 0.0012 0.001 0.0008 0.0013 0.0485 0.0017 0.002 0.0011 0.3051 0.0251 0.0203 0.002 0.0017 0.0002 0.0037 0.0009
Nucleolus 0.0028 0 0.0001 0.0001 0 0.0004 0.001 0.0003 0.0009 0 0.0003 0.0011 0.0021 0.0001 0.0002 0 0 0
Nucleus 0.0349 0.0028 0.0028 0.0025 0.0025 0.0067 0.0914 0.0031 0.0031 0.0034 0.0605 0.0178 0.0158 0.0029 0.0037 0.0024 0.0182 0.0072
Peroxisomes 0.0061 0.0001 0.0002 0.0002 0 0.0094 0.0333 0.0017 0.0077 0 0.0002 0.0038 0.0122 0.0004 0.0049 0 0 0.0005
Punctate Nuclear 0.0456 0.0003 0.0008 0.001 0.0004 0.0015 0.0131 0.0035 0.0116 0.0002 0.0038 0.0093 0.0127 0.0014 0.0051 0.0001 0.0003 0.0005
Vacuole 0.008 0.0072 0.0006 0.0005 0.001 0.0004 0.002 0.0007 0.006 0.0012 0.0031 0.0038 0.014 0.0006 0.0009 0 0.0006 0.0004
Vacuole Periphery 0.0066 0.0034 0.0001 0.0001 0.0001 0.0002 0.0018 0.0002 0.0013 0.0001 0.0071 0.0026 0.0028 0.0002 0.0008 0 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.0095 31.2789 45.341 48.6883 49.0586 38.2237 44.681 51.8744 54.6743 55.2016
Translational Efficiency 1.0394 1.3707 0.8398 0.8886 0.7916 1.1691 0.9842 0.7034 0.7952 0.7592

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1412 154 1752 506 123 2061 363 721 1535 2215 2115 1227

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 949.03 1209.31 1355.82 1247.63 1092.02 1137.41 1371.01 1532.11 960.49 1142.41 1358.43 1414.79
Standard Deviation 163.80 222.74 232.19 231.80 187.31 211.14 220.54 334.30 170.29 212.76 230.30 327.78
Intensity Change Log 2 — 0.349659 0.514640 0.394665 — 0.058753 0.328240 0.488521 — 0.201334 0.417913 0.445638

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000233 0.000280 0.000937 0.000921 0.001178 0.000868 0.000440 0.000326 0.000309 0.000827 0.000852 0.000571
Bud Neck 0.001337 0.001253 0.002368 0.003484 0.002364 0.001333 0.001997 0.019077 0.001419 0.001328 0.002304 0.012647
Bud Site 0.008349 0.007600 0.012858 0.048080 0.031323 0.007489 0.008614 0.026729 0.010190 0.007496 0.012130 0.035534
Cell Periphery 0.000073 0.000090 0.000065 0.000156 0.000265 0.000055 0.000165 0.000043 0.000088 0.000058 0.000082 0.000090
Cytoplasm 0.811122* 0.788630* 0.710310* 0.572200* 0.683636* 0.845499* 0.539069* 0.789144* 0.800906* 0.841545* 0.680920* 0.699679*
Cytoplasmic Foci 0.062410 0.066496 0.006845 0.011664 0.103005 0.036432 0.012724 0.003499 0.065663 0.038522 0.007854 0.006866
Eisosomes 0.000011 0.000018 0.000006 0.000020 0.000033 0.000010 0.000037 0.000008 0.000013 0.000010 0.000011 0.000013
Endoplasmic Reticulum 0.001981 0.000701 0.000462 0.001436 0.005218 0.000383 0.001949 0.000036 0.002240 0.000405 0.000718 0.000613
Endosome 0.002072 0.002622 0.001084 0.003181 0.006223 0.002874 0.002412 0.000796 0.002404 0.002857 0.001312 0.001779
Golgi 0.001144 0.000576 0.000789 0.000566 0.003727 0.002217 0.000165 0.000290 0.001351 0.002103 0.000682 0.000404
Lipid Particles 0.000598 0.000683 0.000512 0.001243 0.002559 0.000633 0.001334 0.000127 0.000756 0.000637 0.000653 0.000587
Mitochondria 0.002869 0.000768 0.001218 0.000493 0.002790 0.000592 0.000195 0.005405 0.002863 0.000605 0.001043 0.003379
Mitotic Spindle 0.006313 0.005694 0.014175 0.055498 0.034280 0.008812 0.009038 0.018577 0.008554 0.008596 0.013293 0.033803
None 0.004531 0.006155 0.011201 0.009058 0.001586 0.003951 0.012918 0.010104 0.004295 0.004104 0.011496 0.009673
Nuclear Periphery 0.000245 0.000208 0.000299 0.000800 0.002453 0.000106 0.000765 0.000103 0.000422 0.000113 0.000379 0.000391
Nuclear Periphery Foci 0.002793 0.002891 0.000957 0.003668 0.004511 0.001733 0.005250 0.000028 0.002931 0.001814 0.001693 0.001529
Nucleolus 0.000537 0.000113 0.000118 0.000142 0.001337 0.000241 0.000230 0.000278 0.000601 0.000232 0.000138 0.000222
Nucleus 0.055648 0.061478 0.213280* 0.131730* 0.044942 0.047435 0.201195* 0.101704 0.054790 0.048411 0.211206* 0.114086
Peroxisomes 0.000741 0.000476 0.000854 0.000664 0.001201 0.001254 0.000147 0.001752 0.000778 0.001200 0.000733 0.001303
Vacuole 0.034933 0.047515 0.021420 0.153023 0.056474 0.037376 0.201232* 0.021587 0.036659 0.038081 0.052282 0.075790
Vacuole Periphery 0.002062 0.005752 0.000241 0.001973 0.010896 0.000706 0.000124 0.000387 0.002770 0.001057 0.000221 0.001041

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.98 -3.55 -4.59 -2.58 1.16 0.66 1.55 2.36 1.47 3.49 -1.31 -3.29 -1.08 1.09 2.64
Bud Neck -0.33 -3.40 -6.28 -5.95 -2.55 2.35 -1.35 -4.04 -4.69 -3.79 0.67 -3.94 -5.20 -5.37 -4.24
Bud Site -0.05 -1.15 -1.92 -1.87 -1.26 3.21 2.65 2.36 -2.07 -0.64 1.91 -0.17 -1.42 -2.96 -1.31
Cell Periphery -1.78 -2.48 -7.66 -4.07 -6.72 5.05 1.82 5.76 3.62 13.13 3.34 -2.49 -0.25 -3.80 2.37
Cytoplasm 2.71 20.92 27.96 14.69 16.01 -5.60 8.99 -2.26 9.72 -20.93 -3.76 24.67 18.59 22.86 -0.89
Cytoplasmic Foci -0.49 20.68 17.98 6.72 -4.57 5.33 7.52 7.97 20.59 6.23 8.81 21.85 21.96 19.50 0.51
Eisosomes -1.78 2.73 -10.15 -1.59 -12.73 4.03 -1.24 4.13 0.49 12.77 2.21 -1.17 -3.15 -5.57 -2.46
Endoplasmic Reticulum 2.45 3.19 1.57 -1.81 -4.56 2.76 1.69 2.97 5.70 10.40 4.23 3.22 3.88 -1.80 3.58
Endosome -1.06 5.25 2.93 2.37 -2.55 2.46 2.76 4.14 5.49 8.36 -0.50 5.58 7.27 4.92 0.98
Golgi 2.36 2.58 3.93 1.92 1.16 1.04 2.38 2.40 3.83 0.94 -1.00 3.45 4.73 3.84 1.36
Lipid Particles -1.32 -2.30 -11.53 -6.90 -9.23 1.65 0.70 1.98 1.94 11.83 0.61 -1.77 -2.34 -2.27 -0.70
Mitochondria 2.26 2.38 3.01 0.51 1.75 1.18 1.34 0.01 -2.74 -3.17 2.89 2.65 1.35 -2.46 -2.00
Mitotic Spindle -0.49 -3.08 -4.31 -3.36 -2.89 1.93 1.71 1.79 -0.46 0.18 -0.12 -2.02 -3.07 -3.08 -1.59
None -0.58 -4.08 -1.21 -0.15 3.66 -5.81 -5.63 -15.55 -11.66 -1.48 0.55 -4.58 -4.35 -9.67 0.89
Nuclear Periphery -0.06 -5.54 -12.25 -11.12 -10.38 1.07 0.43 0.96 -8.75 15.19 1.58 -1.99 -2.23 -17.34 -1.01
Nuclear Periphery Foci -0.81 3.67 -1.06 0.24 -6.75 3.58 -0.94 6.32 11.47 9.35 2.62 2.38 4.37 3.04 4.06
Nucleolus 2.96 2.80 2.55 -3.39 -2.62 1.31 1.31 1.15 -1.50 -3.44 2.45 3.05 2.27 -0.84 -5.85
Nucleus -1.18 -37.98 -12.99 -8.93 12.29 -0.95 -14.02 -10.51 -16.77 6.77 1.11 -40.48 -19.26 -21.20 16.12
Peroxisomes 1.06 1.06 2.22 2.50 1.63 -0.29 2.90 0.16 0.45 -2.62 -1.12 1.48 0.30 1.45 -1.25
Vacuole -3.13 -6.71 -22.82 -15.32 -20.67 1.66 -13.39 0.41 -2.60 15.65 -4.01 -13.88 -18.44 -16.78 -8.62
Vacuole Periphery -0.55 3.97 3.20 0.96 -0.78 1.31 1.38 1.37 1.86 -0.71 2.25 3.54 3.36 1.68 -0.90
Standard name
Human Ortholog
Description Phosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Erg8

Erg8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available