Standard name
Human Ortholog
Description GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.06 0.06 0.07 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.06 0.09 0.07 0.12 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.99 0.95 0.86 0.7 0.68 0.63 0.66 0.66 0.57 0.99 0.98 0.99 0.94 0.91 0.97 0.97 0.97 0.9 0.93 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0.06 0.09 0.11 0.22 0.23 0.13 0.12 0.21 0 0 0 0.07 0 0 0 0 0.08 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.15 0.09 0.08 0.21 0.18 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 6 11 11 13 8 11 10 0 0 0 1 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 1 3 1 1 1 0 0 0 1 0 0 0 0 2 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 3 0 4 6 10 12 18 13 18 17 1 13 14 9 9 8 0 0 0 0 0 0
Cytoplasm 291 127 113 87 109 138 141 131 123 129 96 89 341 368 417 214 196 174 288 126 104 226 273 287
Endoplasmic Reticulum 0 1 4 2 7 15 22 42 45 26 18 32 4 5 4 15 8 6 1 0 8 3 3 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
Golgi 0 0 0 0 0 1 2 1 3 3 1 1 0 0 0 3 4 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 1 7 30 17 16 41 27 40 1 0 0 0 5 3 0 0 0 1 0 0
Nucleus 1 0 0 0 0 1 0 1 0 1 1 0 0 1 0 1 1 0 0 0 0 1 1 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 0 1 8 1 0 5 2 0 0 2 1 6 16 4 0 1 0 2 3 3
Unique Cell Count 294 128 116 88 115 161 200 192 195 195 146 156 344 374 422 228 215 180 297 131 116 243 287 302
Labelled Cell Count 297 129 120 89 121 176 227 217 219 228 174 189 347 391 436 250 239 197 297 131 116 243 287 302


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.7 43.8 30.2 28.1 25.6 23.8 22.4 21.5 20.3 19.5 18.3 18.7 34.0 32.3 30.9 29.5 27.7 29.6 34.6 35.0 37.6
Std Deviation (1e-4) 5.6 7.8 5.9 5.1 4.9 5.0 4.7 4.8 4.1 4.7 4.4 4.3 7.2 6.5 6.1 7.8 7.3 7.8 8.6 8.9 8.8
Intensity Change (Log2) – – – -0.1 -0.24 -0.35 -0.43 -0.49 -0.58 -0.63 -0.72 -0.69 0.17 0.1 0.03 -0.03 -0.13 -0.03 0.2 0.21 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.4 2.3 1.6 2.9 2.6 0 0 0 0 0 0
Cytoplasm 0.7 -1.0 -3.3 -5.8 -6.1 -6.8 -6.4 -6.3 -7.5 1.4 0.7 1.1 -1.4 -2.2 -0.4
Endoplasmic Reticulum 0 0.9 1.9 2.4 4.4 4.6 2.9 2.6 4.1 0 0 0 1.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.4 3.3 3.2 5.3 4.9 5.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.9032 28.1895 24.1652 22.1171 19.4096 24.7561 21.194 34.247 30.9507 26.2007 31.6798 30.5076 25.1576 32.3269 29.5503 28.8285 25.6797 29.319
Actin 0.0094 0.0008 0.0055 0.0012 0.0013 0.0011 0.0235 0.0002 0.0012 0.0409 0 0.0014 0.0167 0.0007 0.0004 0.001 0.0316 0.0008
Bud 0.0007 0.0004 0.0007 0.0017 0.0007 0.0007 0.0012 0.0002 0.0006 0.0015 0.0001 0.0009 0.0006 0.0002 0.0003 0.0008 0.0014 0.0002
Bud Neck 0.0005 0.0004 0.0018 0.0009 0.0007 0.0014 0.0009 0.0002 0.0005 0.0005 0.0004 0.0012 0.0013 0.0003 0.0004 0.0007 0.0008 0.0011
Bud Periphery 0.0012 0.0001 0.0006 0.0011 0.0004 0.0008 0.0012 0.0001 0.0002 0.0007 0 0.0006 0.0008 0.0001 0.0001 0.0002 0.0011 0.0001
Bud Site 0.0008 0.0004 0.0007 0.0004 0.0004 0.0001 0.0037 0.0002 0.0004 0.0027 0 0.0001 0.0062 0.0005 0.0002 0.0003 0.0021 0
Cell Periphery 0.003 0.0003 0.0017 0.0007 0.0002 0.0006 0.0004 0.0003 0.001 0.0002 0 0.0006 0.0016 0.0005 0.0003 0.0003 0.0006 0.0002
Cytoplasm 0.8112 0.9537 0.8834 0.8239 0.7599 0.8557 0.7453 0.9745 0.9379 0.7802 0.9708 0.9125 0.8313 0.9637 0.9372 0.8763 0.7912 0.8798
Cytoplasmic Foci 0.016 0.0053 0.0092 0.0081 0.0323 0.0099 0.0251 0.0015 0.0052 0.0302 0.0018 0.0081 0.0087 0.003 0.0053 0.0078 0.0153 0.0056
Eisosomes 0.0002 0 0.0001 0 0 0 0.0002 0 0 0.0002 0 0 0.0008 0 0 0 0.0006 0
Endoplasmic Reticulum 0.0438 0.0199 0.0313 0.0087 0.0231 0.051 0.0265 0.0096 0.0085 0.0115 0.0034 0.023 0.0524 0.0116 0.0224 0.0114 0.0122 0.0627
Endosome 0.0385 0.0096 0.0244 0.0371 0.0849 0.0401 0.0421 0.0024 0.0136 0.0396 0.0058 0.0284 0.0139 0.0032 0.0106 0.0289 0.0451 0.0193
Golgi 0.0116 0.0024 0.0042 0.0034 0.0067 0.0079 0.0188 0.0002 0.0029 0.0167 0.0001 0.0029 0.0034 0.0017 0.0008 0.0008 0.0128 0.0032
Lipid Particles 0.0056 0 0.0005 0.0002 0.0273 0.0003 0.015 0.0001 0.0004 0.0043 0 0.0001 0.0063 0.0009 0.0003 0.0002 0.0098 0.0001
Mitochondria 0.0096 0.0005 0.004 0.0024 0.004 0.0127 0.0124 0.0001 0.0049 0.0156 0.0001 0.0031 0.0087 0.003 0.0003 0.0003 0.0119 0.0025
None 0.0016 0.0002 0.0004 0.0003 0.0016 0.0005 0.005 0.0001 0.0002 0.0037 0.0001 0.0004 0.0014 0.0001 0.0003 0.0003 0.0036 0.0006
Nuclear Periphery 0.0042 0.001 0.0014 0.0031 0.0232 0.0043 0.0288 0.0012 0.0012 0.0096 0.0014 0.0027 0.0131 0.0008 0.0026 0.0049 0.0241 0.0075
Nucleolus 0.0003 0 0 0 0.0002 0.0001 0.0007 0 0 0.002 0 0 0.0002 0 0 0 0.0009 0
Nucleus 0.0022 0.0007 0.0009 0.0017 0.0031 0.001 0.0167 0.0011 0.001 0.0196 0.001 0.0014 0.0027 0.0006 0.001 0.002 0.0084 0.0015
Peroxisomes 0.0085 0 0.0065 0.0001 0.0125 0.0001 0.0037 0 0.0001 0.0044 0 0.0001 0.0012 0.002 0 0.0001 0.0011 0
Punctate Nuclear 0.0042 0 0.0001 0.0001 0.0012 0.0002 0.0134 0 0.0001 0.005 0 0.0001 0.001 0 0.0001 0.0001 0.0036 0
Vacuole 0.0226 0.0036 0.0189 0.0951 0.0116 0.0081 0.0126 0.0068 0.0181 0.008 0.0118 0.0086 0.0242 0.0064 0.0148 0.0507 0.0151 0.0092
Vacuole Periphery 0.0043 0.0007 0.0037 0.0098 0.0048 0.0033 0.0031 0.001 0.002 0.0028 0.0031 0.0039 0.0034 0.0007 0.0025 0.0128 0.0067 0.0054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 174.4353 149.0846 181.1895 150.3165 141.4249 143.2551 127.5173 131.7106 163.196 130.9841
Translational Efficiency 0.9466 1.068 0.8881 1.3002 1.0411 1.1239 1.1696 0.9982 0.8766 1.0366

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
105 96 1753 1783 1656 1615 1463 1328 1761 1711 3216 3111

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2346.26 2814.02 2912.45 3237.85 2190.78 2468.01 2897.98 3016.11 2200.05 2487.42 2905.87 3143.20
Standard Deviation 379.93 483.75 445.96 584.10 434.25 440.17 442.99 606.18 432.77 449.84 444.67 603.67
Intensity Change Log 2 — 0.262270 0.311870 0.464673 — 0.171904 0.403603 0.461244 — 0.219341 0.356894 0.463019

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000359 0.000549 0.000161 0.000831 0.000076 0.000131 0.000323 0.000744 0.000093 0.000155 0.000234 0.000794
Bud Neck 0.001444 0.003070 0.001277 0.001368 0.000214 0.000413 0.002600 0.001577 0.000287 0.000562 0.001879 0.001457
Bud Site 0.015135 0.017852 0.004667 0.009564 0.004114 0.005337 0.006530 0.011412 0.004771 0.006039 0.005514 0.010353
Cell Periphery 0.017665 0.011353 0.007132 0.029118 0.001704 0.002153 0.005050 0.024007 0.002656 0.002669 0.006185 0.026936
Cytoplasm 0.737714* 0.756006* 0.845340* 0.739549* 0.820178* 0.907284* 0.805448* 0.738797* 0.815261* 0.898796* 0.827192* 0.739228*
Cytoplasmic Foci 0.020463 0.025524 0.006611 0.002833 0.013508 0.011986 0.009055 0.004121 0.013922 0.012746 0.007723 0.003383
Eisosomes 0.000004 0.000005 0.000002 0.000006 0.000001 0.000001 0.000003 0.000006 0.000001 0.000001 0.000002 0.000006
Endoplasmic Reticulum 0.001890 0.000896 0.000893 0.000618 0.001858 0.001312 0.000740 0.000569 0.001860 0.001289 0.000823 0.000597
Endosome 0.005085 0.003431 0.001129 0.000567 0.001063 0.001596 0.001710 0.001652 0.001303 0.001699 0.001393 0.001030
Golgi 0.068261 0.056261 0.017563 0.008805 0.038214 0.024518 0.018305 0.012249 0.040006 0.026299 0.017900 0.010275
Lipid Particles 0.026704 0.018119 0.012709 0.026011 0.001609 0.005616 0.014956 0.025820 0.003105 0.006317 0.013731 0.025930
Mitochondria 0.000048 0.000322 0.000085 0.000200 0.000226 0.000165 0.000058 0.000296 0.000215 0.000173 0.000072 0.000241
Mitotic Spindle 0.002793 0.018975 0.001540 0.000576 0.001786 0.001118 0.001884 0.000651 0.001846 0.002120 0.001697 0.000608
None 0.000348 0.000868 0.000748 0.000453 0.001444 0.000932 0.001093 0.000670 0.001378 0.000929 0.000905 0.000546
Nuclear Periphery 0.000156 0.000663 0.000241 0.000182 0.000157 0.000059 0.000268 0.000177 0.000157 0.000093 0.000253 0.000180
Nuclear Periphery Foci 0.011822 0.010612 0.007977 0.004154 0.005199 0.004504 0.008962 0.004851 0.005594 0.004846 0.008425 0.004452
Nucleolus 0.000067 0.000709 0.000042 0.000127 0.000045 0.000037 0.000061 0.000128 0.000046 0.000075 0.000051 0.000128
Nucleus 0.005430 0.009089 0.007498 0.006209 0.004089 0.003415 0.012085 0.006149 0.004169 0.003733 0.009584 0.006184
Peroxisomes 0.000165 0.000174 0.000034 0.000125 0.000014 0.000038 0.000038 0.000109 0.000023 0.000045 0.000036 0.000118
Vacuole 0.059353 0.037165 0.072496 0.157058 0.015704 0.017960 0.101550 0.155974 0.018307 0.019038 0.085713 0.156595
Vacuole Periphery 0.025093 0.028359 0.011858 0.011644 0.088798 0.011425 0.009282 0.010038 0.084999 0.012375 0.010686 0.010959

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.05 2.86 -1.06 -0.59 -1.58 -3.23 -3.67 -2.56 -2.35 -1.22 -3.23 -4.40 -2.72 -2.48 -1.93
Bud Neck -2.26 -0.25 -0.40 2.23 -0.19 -4.42 -13.46 -11.80 -9.97 5.97 -4.53 -14.76 -16.65 -11.36 4.53
Bud Site -0.55 2.84 1.32 1.86 -5.26 -1.71 -4.42 -6.64 -5.28 -3.19 -1.72 -2.51 -7.17 -5.19 -5.87
Cell Periphery 1.19 2.30 -2.17 -5.64 -13.19 -1.86 -8.18 -14.41 -14.08 -11.90 -0.08 -7.30 -21.12 -21.56 -17.74
Cytoplasm -0.42 -3.56 0.15 0.69 11.71 -10.81 3.75 8.69 19.16 5.05 -10.43 0.45 10.27 24.03 11.82
Cytoplasmic Foci -1.25 5.00 6.57 5.80 8.38 1.22 2.87 10.66 8.40 7.82 0.89 6.49 13.85 11.14 11.36
Eisosomes -0.40 2.47 -1.73 -0.79 -4.90 -4.19 -9.11 -4.09 -3.66 -1.95 -3.55 -10.14 -7.25 -6.40 -4.41
Endoplasmic Reticulum 1.16 1.29 1.73 0.78 1.91 0.93 2.06 2.40 3.13 0.87 1.03 2.02 2.53 3.23 1.99
Endosome 0.75 1.98 2.31 3.12 6.73 -2.39 -5.18 -1.55 0.03 0.75 -1.64 -1.33 1.45 2.75 3.05
Golgi 0.78 4.59 5.46 4.51 8.53 5.33 8.81 11.33 6.39 3.78 5.30 10.39 14.37 9.42 8.50
Lipid Particles 1.21 2.16 0.11 -2.12 -7.14 -6.67 -14.53 -15.41 -12.47 -5.39 -4.59 -14.12 -19.48 -16.50 -8.90
Mitochondria -1.50 -2.68 -2.30 0.64 -1.76 0.80 5.61 -0.49 -0.88 -1.86 0.58 5.18 -0.32 -0.70 -2.54
Mitotic Spindle -1.69 0.66 1.72 1.93 3.71 2.20 -0.54 4.18 2.04 3.04 -0.53 -0.41 4.90 2.63 4.76
None -3.54 -4.95 -1.69 2.92 4.20 2.33 0.94 3.46 1.31 0.90 2.18 2.07 4.80 2.58 1.72
Nuclear Periphery -1.83 -3.28 -0.86 1.72 2.52 6.77 -3.28 -0.72 -2.64 1.56 2.98 -4.99 -1.22 -3.19 2.65
Nuclear Periphery Foci 0.45 1.89 4.34 3.22 7.33 1.22 -6.97 0.30 -0.86 6.91 1.34 -6.57 2.36 0.72 10.17
Nucleolus -1.04 1.34 -2.57 0.95 -15.18 0.58 -3.85 -9.97 -7.10 -7.40 -0.81 -1.76 -12.91 -1.42 -15.49
Nucleus -0.80 -0.94 -0.55 0.58 1.24 0.78 -11.03 -4.36 -5.60 7.65 0.47 -10.32 -5.34 -6.59 6.70
Peroxisomes -0.13 2.68 0.88 0.57 -4.85 -5.24 -5.31 -11.48 -7.63 -6.17 -2.88 -3.60 -8.29 -5.40 -6.81
Vacuole 1.54 -1.46 -8.34 -12.02 -14.29 -1.40 -19.75 -24.86 -24.02 -6.53 -0.57 -23.71 -36.75 -35.61 -14.58
Vacuole Periphery -0.35 2.39 2.51 2.19 0.60 16.53 17.35 17.02 1.22 -0.57 16.32 17.17 17.12 1.42 -0.00
Standard name
Human Ortholog
Description GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rna1

Rna1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rna1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available