Standard name
Human Ortholog
Description Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0.05 0 0.08 0 0 0 0 0 0
Cytoplasm 0.88 0.98 – 0.97 0.99 0.95 0.92 – 0.71 0.77 0.65 0.67 – – 0.9 0.93 0.97 0.96 0.73 0.85 0.84 0.9 0.99 0.82
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – 0.05 0.08 0 0.08 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – 0.05 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0 – 0.07 0 0 0 – 0.38 0.22 0.46 0.36 – – 0 0 0 0 0.17 0.06 0.06 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0.05 – – 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 1 0 0 0 – 2 2 1 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 1 2 – 1 0 0 0 – 3 6 2 3 – – 0 0 1 1 0 0 0 0 0 1
Bud Neck 0 1 – 1 2 1 3 – 3 2 0 2 – – 0 0 1 1 0 1 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 1 – – 0 0 0 0 – – – – – –
Cell Periphery 1 0 – 1 2 1 1 – 0 1 0 2 – – 0 19 11 28 0 0 0 0 0 0
Cytoplasm 92 155 – 65 173 110 178 – 60 141 73 125 – – 18 328 271 335 78 138 14 7 25 40
Endoplasmic Reticulum 2 1 – 0 1 0 4 – 2 1 3 3 – – 1 29 12 29 0 1 0 0 0 0
Endosome 0 0 – 0 1 0 2 – 1 1 0 1 – – 0 8 2 5 2 2 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – 1 3 1 0 0 0 0 0 0 0
Mitochondria 27 0 – 5 0 5 7 – 32 40 51 68 – – 0 5 2 4 18 9 1 0 0 1
Nucleus 1 1 – 2 0 0 2 – 3 3 1 3 – – 0 0 3 1 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 1 3 2 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 1 0 – 0 0 0 0 – – 0 2 0 0 0 0 0 0 0 0
SpindlePole 0 1 – 0 1 0 5 – 0 2 2 3 – – 0 0 1 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 1 2 – 0 6 2 9 – – 0 12 3 4 1 7 1 0 0 1
Unique Cell Count 105 158 67 174 116 194 85 182 112 187 20 351 280 348 108 163 17 8 26 49
Labelled Cell Count 124 161 76 180 119 204 107 208 137 220 20 406 308 409 108 163 17 8 26 49


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.9 – 4.7 5.6 4.7 4.4 – 4.0 4.5 4.1 4.6 – – 7.4 6.5 6.4 6.0 – – 6.4
Std Deviation (1e-4) 0.5 4.4 – 1.4 2.0 2.3 1.1 – 2.0 1.6 1.9 2.1 – – 2.9 1.5 1.6 1.4 – – 2.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0676 -0.423 -0.3499 -0.5205 0.1674 -0.3096 0.1353 0.7408 0.0828 -0.1123 -0.2371 -0.7514 -2.0257 -1.6546 -1.6728 -2.0254 -1.907 -0.9871
Actin 0.0379 0.0006 0.0063 0.0101 0.01 0.0039 0.0499 0.0004 0.0168 0.0339 0 0.0003 0.0391 0.0001 0.0188 0.0003 0.0051 0.001
Bud 0.002 0.0076 0.0044 0.0007 0.0018 0.0058 0.0008 0.0003 0.0016 0.0003 0 0 0.0031 0.0001 0.0021 0 0.0002 0.0026
Bud Neck 0.002 0.0004 0.0006 0.0002 0.0012 0.0013 0.0064 0.0001 0.0005 0.0008 0 0.0001 0.0012 0 0.0004 0 0.0004 0.0003
Bud Periphery 0.0021 0.0099 0.0022 0.0014 0.0018 0.0056 0.0009 0.0002 0.0015 0.0005 0 0.0001 0.0036 0.0001 0.0026 0 0.0004 0.0078
Bud Site 0.0102 0.0295 0.0072 0.0006 0.0081 0.0048 0.0086 0.0033 0.01 0.0012 0 0 0.0046 0.0004 0.0018 0.0001 0.0007 0.0002
Cell Periphery 0.0004 0.0005 0.0005 0.0001 0.0002 0.0002 0.0005 0.0002 0.0002 0.0002 0 0.0003 0.0003 0.0001 0.0002 0 0.0001 0.0002
Cytoplasm 0.0479 0.1134 0.0681 0.074 0.0602 0.1119 0.1088 0.2931 0.1708 0.2107 0.1361 0.2702 0.0722 0.1852 0.1059 0.0958 0.1375 0.1511
Cytoplasmic Foci 0.025 0.0136 0.029 0.0121 0.0456 0.0159 0.0311 0.007 0.0176 0.0218 0.0012 0.0048 0.0276 0.0092 0.0087 0.0084 0.0195 0.0128
Eisosomes 0.0007 0.0004 0.0005 0.0001 0.0002 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0027 0.001 0.0017 0.0007 0.0012 0.0012 0.0031 0.0017 0.002 0.0028 0.0002 0.0089 0.0018 0.0013 0.0012 0.001 0.0045 0.0007
Endosome 0.0542 0.0016 0.0302 0.0154 0.0594 0.0107 0.0215 0.0052 0.0086 0.0704 0.0001 0.002 0.0092 0.0023 0.0034 0.0015 0.043 0.0063
Golgi 0.0249 0.0002 0.0108 0.0146 0.0321 0.0056 0.0115 0.0008 0.0046 0.0147 0 0.0002 0.007 0.0001 0.0023 0.0001 0.0161 0.0018
Lipid Particles 0.0221 0.0009 0.0088 0.0041 0.0254 0.0018 0.0183 0.0005 0.0032 0.011 0.0001 0.0003 0.0165 0.0002 0.0036 0.0004 0.0406 0.0107
Mitochondria 0.0252 0.0011 0.0195 0.0671 0.0204 0.026 0.0284 0.0002 0.0027 0.0037 0 0.0005 0.0083 0.0002 0.0015 0.0001 0.009 0.0048
None 0.6912 0.8034 0.781 0.7932 0.662 0.7899 0.6262 0.6828 0.7441 0.6086 0.8616 0.7098 0.7726 0.798 0.8266 0.8897 0.6971 0.789
Nuclear Periphery 0.006 0.0008 0.001 0.0003 0.0029 0.0022 0.0059 0.0007 0.0006 0.0052 0.0001 0.0004 0.001 0.0004 0.0006 0.0003 0.0022 0.0007
Nucleolus 0.0042 0.0013 0.0015 0.0001 0.004 0.0003 0.013 0.0002 0.0004 0.0003 0 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0012
Nucleus 0.0049 0.0066 0.0031 0.0003 0.0046 0.0026 0.0077 0.0008 0.0008 0.0012 0.0001 0.0004 0.0018 0.0005 0.0005 0.0003 0.0005 0.0008
Peroxisomes 0.0101 0.0008 0.0137 0.0026 0.0249 0.0047 0.0364 0.0001 0.0109 0.0032 0 0 0.0229 0.0001 0.0165 0.0002 0.0181 0.0032
Punctate Nuclear 0.0154 0.0041 0.0049 0.0003 0.0164 0.0033 0.0165 0.001 0.001 0.0056 0.0001 0.0001 0.0046 0.0002 0.0022 0.0005 0.0016 0.0009
Vacuole 0.0075 0.0017 0.0039 0.001 0.0104 0.0012 0.0028 0.0012 0.002 0.0031 0.0001 0.001 0.0016 0.001 0.0007 0.0009 0.0023 0.0024
Vacuole Periphery 0.0033 0.0003 0.0012 0.0008 0.0072 0.0009 0.0011 0.0001 0.0003 0.0009 0 0.0002 0.0004 0.0001 0.0001 0.0001 0.0008 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4944 6.7329 7.9105 23.0629 7.7308 23.8374 12.3389 21.442 35.2766 18.6866
Translational Efficiency 1.2671 0.9106 1.4321 1.1282 1.3705 1.08 0.9163 0.8753 0.9006 0.8659

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
374 878 670 331 2101 1779 645 395 2475 2657 1315 726

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.47 655.13 1004.46 900.10 670.26 652.08 858.18 848.86 671.20 653.09 932.71 872.22
Standard Deviation 160.87 103.90 276.70 183.09 77.85 85.06 126.90 172.69 95.19 91.73 228.60 179.33
Intensity Change Log 2 — -0.046245 0.570322 0.412059 — -0.039672 0.356559 0.340806 — -0.042970 0.467888 0.377037

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000241 0.000405 0.000592 0.001009 0.000089 0.000886 0.000258 0.000481 0.000112 0.000727 0.000428 0.000721
Bud Neck 0.017139 0.043000 0.003590 0.007926 0.016094 0.056707 0.003808 0.006361 0.016252 0.052178 0.003697 0.007075
Bud Site 0.029651 0.017902 0.014894 0.043637 0.007097 0.021506 0.012328 0.034086 0.010505 0.020315 0.013636 0.038441
Cell Periphery 0.000255 0.000159 0.000205 0.000165 0.000118 0.000255 0.000107 0.001204 0.000138 0.000223 0.000157 0.000730
Cytoplasm 0.611881* 0.336074* 0.492161* 0.495882* 0.502016* 0.289694* 0.487219* 0.501484* 0.518618* 0.305020* 0.489737* 0.498930*
Cytoplasmic Foci 0.158893 0.296812* 0.019571 0.037485 0.219082* 0.254232* 0.011882 0.043165 0.209987* 0.268302* 0.015799 0.040575
Eisosomes 0.000158 0.000188 0.000034 0.000062 0.000129 0.000205 0.000037 0.000056 0.000133 0.000199 0.000035 0.000058
Endoplasmic Reticulum 0.001492 0.000815 0.003897 0.002014 0.000664 0.000874 0.002563 0.001326 0.000789 0.000855 0.003243 0.001640
Endosome 0.008566 0.040238 0.003123 0.007111 0.009801 0.050873 0.002408 0.013201 0.009615 0.047358 0.002772 0.010425
Golgi 0.001254 0.008780 0.000923 0.018767 0.002477 0.010606 0.000128 0.002229 0.002292 0.010003 0.000533 0.009769
Lipid Particles 0.006579 0.013319 0.001956 0.001357 0.007490 0.016658 0.001279 0.001194 0.007353 0.015555 0.001624 0.001268
Mitochondria 0.002393 0.022061 0.000450 0.003864 0.005322 0.034911 0.000492 0.001302 0.004879 0.030664 0.000471 0.002470
Mitotic Spindle 0.002179 0.005975 0.006812 0.027034 0.004198 0.015613 0.011584 0.010747 0.003893 0.012428 0.009153 0.018173
None 0.005703 0.003701 0.004132 0.006683 0.003541 0.004907 0.009519 0.009736 0.003868 0.004508 0.006774 0.008344
Nuclear Periphery 0.000374 0.000914 0.001367 0.000814 0.000390 0.000768 0.001488 0.000680 0.000388 0.000816 0.001426 0.000741
Nuclear Periphery Foci 0.000509 0.000505 0.003267 0.002006 0.000308 0.000443 0.001608 0.001420 0.000338 0.000464 0.002453 0.001687
Nucleolus 0.000644 0.002822 0.000241 0.000320 0.001637 0.003743 0.000532 0.000279 0.001486 0.003439 0.000384 0.000298
Nucleus 0.064171 0.077719 0.371541* 0.159760* 0.176006* 0.103598 0.374628* 0.221985* 0.159106* 0.095046 0.373055* 0.193615*
Peroxisomes 0.003022 0.025382 0.000267 0.001922 0.005156 0.022744 0.000436 0.001303 0.004834 0.023616 0.000350 0.001585
Vacuole 0.082638 0.097141 0.070708 0.181102 0.037422 0.106144 0.077531 0.146112 0.044255 0.103169 0.074055 0.162064
Vacuole Periphery 0.002257 0.006089 0.000269 0.001079 0.000964 0.004633 0.000165 0.001651 0.001159 0.005114 0.000218 0.001390

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.32 -4.53 -1.49 -0.73 0.51 -1.67 -6.84 -5.81 0.98 -1.06 -1.93 -8.00 -3.58 0.72 0.31
Bud Neck -6.66 5.08 3.88 11.50 -2.11 -14.81 11.95 8.15 18.52 -0.48 -16.50 13.52 9.51 21.82 -1.62
Bud Site 2.61 3.26 1.09 -1.13 -1.91 -7.04 -0.30 -2.31 0.94 -2.01 -5.74 -0.06 -2.86 0.14 -2.67
Cell Periphery 3.68 1.23 4.83 2.31 4.82 -6.80 0.12 -0.99 -0.49 -1.00 -5.50 -2.30 -0.86 -0.29 -0.66
Cytoplasm 15.97 10.31 11.94 -0.99 3.22 24.90 10.50 7.13 -6.35 -0.99 28.25 12.61 12.01 -5.75 1.62
Cytoplasmic Foci -12.09 15.92 12.35 28.71 -4.08 -5.07 44.07 33.50 36.38 -5.50 -9.71 45.54 35.54 45.65 -6.46
Eisosomes -1.06 3.88 3.06 7.45 -3.09 -6.85 10.32 8.88 14.48 -1.66 -6.43 11.20 8.78 15.74 -3.50
Endoplasmic Reticulum 1.10 -6.35 -1.78 -6.27 6.69 -1.43 -10.94 -6.97 -6.80 5.10 -0.49 -14.59 -7.82 -9.20 8.45
Endosome -13.27 5.72 3.66 15.08 -1.26 -18.03 9.31 2.28 15.31 -1.03 -21.55 9.74 3.70 21.18 -1.32
Golgi -7.25 1.70 -1.00 1.38 -1.18 -9.02 8.38 3.34 10.20 -1.16 -10.85 6.85 0.02 4.99 -1.35
Lipid Particles -3.25 2.55 2.87 7.97 1.72 -7.41 10.79 11.43 13.66 1.92 -8.05 10.22 11.10 15.70 2.66
Mitochondria -7.18 1.66 0.98 8.05 -0.75 -12.04 7.11 6.68 14.52 -0.28 -13.64 7.26 5.92 16.42 -0.80
Mitotic Spindle -2.36 -2.06 -2.90 -1.85 -1.74 -5.24 -1.71 -0.79 3.19 0.79 -5.27 -2.08 -2.48 1.06 -0.80
None 1.21 1.44 0.51 -1.22 -1.82 -1.40 -3.58 -2.53 -0.76 0.53 -0.79 -1.65 -2.09 -1.05 -0.83
Nuclear Periphery -1.49 -9.07 -6.76 -1.97 1.56 -4.34 -19.73 -8.05 -4.64 5.93 -3.37 -23.22 -11.80 -4.78 5.38
Nuclear Periphery Foci -0.21 -8.55 -5.63 -5.61 3.72 -1.37 -8.37 -6.49 -5.90 0.27 -1.67 -11.74 -8.94 -8.19 3.28
Nucleolus -6.37 2.42 1.09 7.24 -1.51 -3.95 2.94 3.46 9.90 1.86 -4.55 3.29 3.34 11.84 0.26
Nucleus -1.81 -23.57 -7.72 -7.17 12.93 13.44 -19.53 -3.06 -8.74 11.46 14.00 -26.61 -3.78 -10.90 16.97
Peroxisomes -11.28 6.03 3.12 12.29 -3.55 -12.51 9.58 7.18 16.22 -1.71 -16.05 10.48 7.77 20.36 -3.48
Vacuole -1.15 -3.63 -15.14 -15.27 -12.95 -16.95 -14.84 -18.57 -13.67 -8.29 -17.01 -17.02 -25.95 -20.34 -15.01
Vacuole Periphery -2.82 1.81 1.73 7.09 -0.61 -11.51 5.01 1.43 10.25 -1.13 -10.26 4.45 2.70 12.92 -1.23
Standard name
Human Ortholog
Description Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
Localization
Cell Percentages cytoplasm (34%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gad1

Gad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available