Standard name
Human Ortholog
Description GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.91 – 1.0 0.95 0.95 0.91 0.89 – 0.81 0.82 0.71 0.77 0.95 1.0 0.97 0.94 0.96 0.92 0.67 0.78 0.76 0.8 0.95 0.92
Endoplasmic Reticulum 0.06 – 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0.05 0.1 0.08 0 0 0 0
Endosome 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0.06 – 0.14 0.12 0.34 0.18 0 0 0 0 0 0 0.1 0 0.07 0 0 0
Nucleus 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0.06 0.06 – 0 0.05 0 0 0 0 0 0.06 0 0.05 0 0 0.06 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 2 2 1 4 – 0 1 1 4 0 0 0 0 3 0 3 1 0 0 0 1
Bud 1 – 0 2 0 1 6 – 2 3 7 7 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 1 – 1 1 2 1 2 – 5 3 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 1 12 6 6 3 – 5 5 2 3 0 0 4 5 2 20 2 0 1 0 0 0
Cytoplasm 132 – 61 313 516 387 580 – 268 386 231 342 55 204 266 133 186 220 117 34 78 8 88 142
Endoplasmic Reticulum 9 – 0 8 13 21 11 – 7 5 7 18 1 3 1 4 7 11 18 3 2 0 0 0
Endosome 0 – 0 0 2 2 7 – 3 8 2 1 2 0 1 0 0 3 1 0 2 0 0 1
Golgi 0 – 0 0 1 0 1 – 0 0 0 0 0 0 1 1 0 0 3 0 0 0 0 0
Mitochondria 5 – 0 1 1 4 39 – 46 57 110 80 0 0 0 5 2 0 18 0 7 0 0 2
Nucleus 1 – 0 3 3 5 8 – 8 4 1 4 0 3 1 0 0 2 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 1 0 2 – 0 6 3 5 0 0 0 1 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 – 0 0 17 25 36 – 14 24 7 20 0 1 8 9 5 11 6 0 6 0 0 1
Unique Cell Count 145 61 328 543 427 651 332 472 326 445 58 205 275 142 193 239 176 45 103 10 93 155
Labelled Cell Count 149 63 342 564 453 699 358 504 373 487 58 211 282 158 205 267 176 45 103 10 93 155


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 – 6.2 5.7 6.3 5.1 5.3 – 4.6 4.8 4.3 4.6 6.3 7.4 7.2 7.4 7.6 7.2 – 5.4 6.2
Std Deviation (1e-4) 0.7 – 0.8 0.9 1.1 0.7 1.0 – 0.9 0.8 1.0 1.1 1.2 1.6 1.2 1.2 1.3 1.1 – 1.0 1.3
Intensity Change (Log2) – – – -0.12 0.03 -0.27 -0.22 – -0.44 -0.38 -0.52 -0.43 0.01 0.25 0.2 0.24 0.29 0.22 – -0.19 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -1.8 -2.5 -2.7 – -3.7 -3.6 -4.9 -4.2 -1.8 -0.5 -1.4 -2.0 -1.5 -2.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2873 1.4419 1.4523 1.0317 0.8092 1.3287 0.7179 1.1361 0.6641 0.3864 0.2332 0.6449 -0.5724 0.0046 -0.2242 -0.2579 0.6084 0.0345
Actin 0.0462 0.0033 0.0408 0.0001 0.041 0.0026 0.0234 0.0003 0.0054 0.0001 0.0002 0.0001 0.0168 0.0012 0.008 0.0008 0.0976 0.0065
Bud 0.0014 0.0038 0.0024 0 0.0014 0.0004 0.0019 0.0002 0.0002 0.0001 0 0 0.0004 0.0004 0.0014 0.0015 0.0051 0.0007
Bud Neck 0.0022 0.0012 0.0012 0.0001 0.0005 0.0017 0.0045 0.0001 0.0002 0.0003 0.0001 0.0003 0.0012 0.0001 0.0005 0.0007 0.0051 0.001
Bud Periphery 0.0048 0.0088 0.0023 0 0.0034 0.0004 0.0016 0.0002 0.0002 0.0002 0 0 0.0005 0.0003 0.0007 0.0014 0.0057 0.0005
Bud Site 0.0043 0.0107 0.0203 0.0001 0.0011 0.0001 0.0141 0.0008 0.0011 0.0001 0 0 0.0032 0.0009 0.0024 0.003 0.0167 0.0005
Cell Periphery 0.0022 0.0007 0.0008 0.0002 0.0001 0.0002 0.0004 0.0004 0.0003 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0002 0.0011 0.0002
Cytoplasm 0.3633 0.5362 0.4606 0.5644 0.5257 0.6505 0.4199 0.5794 0.5225 0.7009 0.4364 0.7072 0.4045 0.6047 0.545 0.6913 0.2643 0.5844
Cytoplasmic Foci 0.0136 0.0101 0.023 0.0082 0.0105 0.0091 0.0329 0.0137 0.0065 0.0085 0.0436 0.0024 0.0922 0.0198 0.0248 0.0095 0.0514 0.0337
Eisosomes 0.0006 0.0002 0.0006 0 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0.0006 0.0001
Endoplasmic Reticulum 0.0261 0.009 0.0111 0.0489 0.0081 0.01 0.0096 0.0092 0.0264 0.0117 0.0066 0.0081 0.0219 0.0151 0.0245 0.02 0.0056 0.0127
Endosome 0.0307 0.0037 0.0186 0.0071 0.0355 0.0074 0.0288 0.0102 0.0127 0.007 0.0302 0.0015 0.0629 0.0105 0.014 0.0237 0.0599 0.0365
Golgi 0.0076 0.0009 0.0114 0.0002 0.0265 0.0019 0.0102 0.0003 0.0054 0.0002 0.0017 0.0001 0.0269 0.0013 0.0099 0.0045 0.1028 0.0437
Lipid Particles 0.0033 0.0009 0.0099 0.0004 0.0268 0.001 0.0356 0.0013 0.0006 0.0003 0.0019 0.0001 0.0421 0.0042 0.0099 0.0005 0.027 0.0186
Mitochondria 0.0124 0.0087 0.0062 0.0003 0.0242 0.0066 0.0071 0.0001 0.0234 0.0002 0.0004 0.0001 0.0088 0.0025 0.0249 0.0195 0.0739 0.0161
None 0.4426 0.3956 0.3682 0.3634 0.2529 0.3002 0.3693 0.3796 0.3884 0.2651 0.476 0.2785 0.263 0.3301 0.313 0.2116 0.2308 0.2025
Nuclear Periphery 0.0179 0.0014 0.0029 0.0025 0.0208 0.0015 0.0027 0.001 0.0016 0.001 0.0005 0.0004 0.02 0.0031 0.0026 0.0032 0.0011 0.0025
Nucleolus 0.0005 0.0002 0.0004 0 0.0006 0.0001 0.0013 0.0001 0 0 0 0 0.0004 0.0002 0.0005 0.0001 0.0006 0.0004
Nucleus 0.0049 0.0016 0.0026 0.0015 0.0023 0.0028 0.002 0.0011 0.0008 0.001 0.0004 0.0006 0.0033 0.0012 0.0016 0.0018 0.0008 0.0021
Peroxisomes 0.001 0.0007 0.0079 0.0001 0.0069 0.0004 0.0229 0 0.0004 0 0.0003 0 0.0231 0.0008 0.01 0.0004 0.0388 0.0167
Punctate Nuclear 0.0028 0.0006 0.0042 0.0006 0.0059 0.0013 0.0014 0.0003 0.0002 0.0002 0.0003 0.0001 0.0031 0.0011 0.0032 0.0005 0.0012 0.0168
Vacuole 0.0084 0.0018 0.0037 0.0016 0.003 0.0014 0.0089 0.0015 0.0032 0.0026 0.0011 0.0004 0.0036 0.002 0.0022 0.0045 0.0072 0.003
Vacuole Periphery 0.0032 0.0001 0.0008 0.0002 0.0025 0.0003 0.0014 0.0001 0.0006 0.0002 0.0002 0 0.0016 0.0002 0.0004 0.0014 0.0028 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.2697 11.8697 15.4138 19.8024 12.0509 16.5884 18.7523 23.6931 27.1664 23.2603
Translational Efficiency 0.5004 0.5781 0.4738 0.5523 0.4297 0.5767 0.5549 0.3816 0.4597 0.388

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1174 400 142 619 1485 1148 1378 122 2659 1548 1520 741

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 698.38 841.51 1075.55 837.81 797.31 720.67 884.55 917.25 753.63 751.89 902.39 850.89
Standard Deviation 79.72 127.88 107.26 209.99 95.74 98.03 109.38 132.05 101.68 118.95 122.52 201.43
Intensity Change Log 2 — 0.268968 0.622990 0.262611 — -0.145802 0.149803 0.202174 — 0.062750 0.390116 0.230709

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000117 0.000516 0.001223 0.001389 0.000394 0.000949 0.000317 0.000317 0.000272 0.000837 0.000402 0.001212
Bud Neck 0.012431 0.018421 0.006308 0.007302 0.011680 0.036552 0.007255 0.005418 0.012012 0.031867 0.007166 0.006992
Bud Site 0.005730 0.019957 0.008258 0.043284 0.007090 0.023836 0.016579 0.011787 0.006490 0.022833 0.015801 0.038098
Cell Periphery 0.000306 0.000183 0.000849 0.000374 0.000413 0.000206 0.000203 0.000129 0.000366 0.000200 0.000264 0.000333
Cytoplasm 0.463300* 0.349327* 0.292036* 0.514636* 0.420940* 0.330888* 0.571215* 0.374287* 0.439643* 0.335653* 0.545134* 0.491529*
Cytoplasmic Foci 0.216887* 0.266206* 0.039072 0.052124 0.223555* 0.300879* 0.029410 0.085577 0.220611* 0.291919* 0.030313 0.057632
Eisosomes 0.000289 0.000164 0.000162 0.000084 0.000378 0.000279 0.000052 0.000098 0.000339 0.000249 0.000063 0.000086
Endoplasmic Reticulum 0.008263 0.020109 0.019917 0.002658 0.061447 0.004077 0.003752 0.001827 0.037965 0.008219 0.005262 0.002521
Endosome 0.022637 0.035643 0.019744 0.009713 0.025957 0.047714 0.003708 0.022759 0.024491 0.044595 0.005206 0.011861
Golgi 0.003532 0.005104 0.000279 0.003492 0.007404 0.009627 0.000262 0.003127 0.005695 0.008459 0.000264 0.003432
Lipid Particles 0.026058 0.012012 0.009412 0.001828 0.027854 0.015537 0.001758 0.002146 0.027061 0.014626 0.002473 0.001881
Mitochondria 0.003867 0.003258 0.000365 0.003491 0.003816 0.012293 0.000716 0.001239 0.003839 0.009958 0.000684 0.003121
Mitotic Spindle 0.000141 0.003900 0.010709 0.019133 0.000268 0.005586 0.019600 0.015988 0.000212 0.005150 0.018770 0.018615
None 0.004955 0.003066 0.000831 0.017669 0.001270 0.000930 0.002466 0.013785 0.002897 0.001482 0.002313 0.017030
Nuclear Periphery 0.001148 0.001790 0.003608 0.000680 0.001624 0.000665 0.001457 0.000773 0.001414 0.000955 0.001658 0.000695
Nuclear Periphery Foci 0.001198 0.004666 0.022604 0.005687 0.003993 0.001202 0.003693 0.002028 0.002759 0.002097 0.005460 0.005085
Nucleolus 0.001321 0.000801 0.000498 0.001211 0.000608 0.001128 0.000180 0.000228 0.000923 0.001043 0.000210 0.001050
Nucleus 0.122048 0.061485 0.226273* 0.150592* 0.080128 0.053285 0.192328* 0.090955 0.098636 0.055404 0.195499* 0.140773*
Peroxisomes 0.003156 0.008826 0.000684 0.001676 0.003312 0.014957 0.000699 0.001242 0.003243 0.013373 0.000698 0.001605
Vacuole 0.100061 0.179851 0.336678* 0.158100 0.114994 0.134873 0.143503 0.365735* 0.108401 0.146495 0.161550 0.192286
Vacuole Periphery 0.002554 0.004715 0.000489 0.004876 0.002874 0.004539 0.000846 0.000554 0.002732 0.004585 0.000813 0.004164

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.56 -8.29 -3.01 -1.77 0.73 -2.83 -0.27 1.38 3.31 1.11 -3.85 -2.38 -2.55 -0.51 -1.60
Bud Neck -2.22 2.20 5.28 5.13 2.08 -9.59 3.59 5.09 12.55 1.83 -9.85 4.60 6.44 13.23 1.40
Bud Site -5.16 -2.99 -5.33 -1.72 -4.21 -8.32 -2.35 -0.39 5.57 1.24 -9.83 -2.93 -5.27 -0.42 -3.59
Cell Periphery 6.26 -2.43 1.06 -3.34 2.57 5.43 7.06 9.26 4.30 5.94 6.72 4.70 4.14 -1.84 -0.39
Cytoplasm 7.62 11.46 3.17 -4.08 -8.14 8.71 -4.30 4.77 1.21 6.39 12.45 -0.47 3.34 -5.07 3.34
Cytoplasmic Foci -3.85 25.30 30.53 20.99 -0.78 -9.41 40.79 19.52 24.93 -3.62 -10.50 52.55 41.46 40.53 -4.53
Eisosomes 6.02 5.39 11.91 4.88 4.50 2.18 11.31 10.02 4.97 -2.58 2.81 15.53 14.01 5.73 -1.29
Endoplasmic Reticulum -2.33 -5.78 4.54 3.43 9.24 13.65 13.68 13.79 1.25 1.62 10.98 13.19 14.14 3.59 5.80
Endosome -4.06 1.65 10.22 10.47 4.98 -7.84 17.96 3.02 6.75 -1.65 -9.17 20.21 12.58 17.10 -1.86
Golgi -0.94 7.63 2.47 2.21 -1.87 -2.24 14.56 5.73 6.63 -0.94 -3.41 16.25 6.21 7.53 -2.22
Lipid Particles 6.07 9.09 14.02 5.86 7.86 7.15 20.09 18.79 10.40 -1.53 8.86 23.32 23.79 12.67 2.87
Mitochondria 0.68 4.13 2.01 1.41 -1.69 -4.43 4.41 4.04 6.59 0.14 -4.22 5.93 2.89 5.81 -1.48
Mitotic Spindle -2.88 -1.53 -3.98 -2.68 -0.98 -3.57 -5.68 -1.39 -0.34 1.31 -4.26 -5.89 -4.20 -2.32 0.50
None 0.83 4.40 -1.92 -2.09 -4.09 1.42 -3.51 -1.18 -1.25 -0.94 1.99 1.52 -3.10 -3.76 -3.54
Nuclear Periphery -1.06 -10.03 -0.11 1.04 10.17 4.19 -2.78 0.69 -2.85 3.11 2.23 -6.63 1.48 -1.32 9.42
Nuclear Periphery Foci -3.38 -7.79 -6.58 -0.74 5.85 6.69 1.09 0.42 -2.71 -0.13 1.77 -5.38 -4.09 -4.90 -0.23
Nucleolus 3.53 6.18 3.92 0.66 -1.37 -2.52 6.69 4.55 4.27 -1.08 -0.72 10.82 2.74 2.41 -4.01
Nucleus 9.36 -5.95 -5.34 -11.26 2.72 7.13 -22.67 0.26 -2.39 12.82 12.88 -20.91 -7.54 -13.07 6.97
Peroxisomes -4.05 8.17 4.90 5.60 -1.39 -11.28 9.38 9.15 13.90 -0.87 -11.94 10.55 7.16 14.26 -1.75
Vacuole -8.39 -13.41 -15.45 -6.31 4.25 -3.54 -15.84 -13.80 -12.99 -9.37 -8.15 -20.20 -18.87 -14.51 -5.60
Vacuole Periphery -1.65 7.24 0.43 1.61 -1.88 -2.14 6.33 7.95 6.04 0.60 -2.80 7.61 1.14 2.76 -1.80
Standard name
Human Ortholog
Description GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
Localization
Cell Percentages cytoplasm (34%), ER (23%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Roy1

Roy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Roy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available