Standard name
Human Ortholog
Description Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.06 0.08 0.08 0.07 0.05 0 0.05 0 0 0.13 0.18 0.2 0.08 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.31 0.17 0.09 0.29 0.3 0.59 0.68 0.54 0.61 0.67 0.69 0.63 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Nucleus 0.9 0.91 0.87 0.86 0.73 0.67 0.68 0.72 0.77 0.73 0.73 0.74 0.84 0.77 0.74 0.83 0.77 0.76 0.88 0.87 0.81 0.75 0.77 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.14 0.07 0.06 0.11 0.12 0.15 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 1 6 16 9 13 14 12 12 0 0 0 1 0 0 1 0 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 1 5 7 11 19 15 26 27 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 18 18 28 32 46 38 22 34 19 18 21 48 58 10 20 17 1 0 3 4 4 6
Endoplasmic Reticulum 0 0 1 0 1 0 0 0 0 1 0 0 0 0 1 4 10 5 0 0 0 1 1 1
Endosome 0 0 1 0 0 0 0 3 0 0 0 0 1 3 8 1 2 1 0 1 2 1 0 3
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 2 0 0 1
Mitochondria 51 23 28 81 106 248 440 375 340 456 383 454 3 3 3 6 11 6 0 2 3 1 1 6
Nucleus 150 124 281 241 254 282 444 500 431 497 407 529 133 206 219 105 145 134 146 121 271 86 187 152
Nuclear Periphery 0 1 1 1 1 1 4 3 2 4 5 6 0 1 2 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 5 10 6 10 12 17 0 0 0 0 0 1 0 0 1 0 1 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 2 2 2 3 3 2 0 0 2 1 0 1 0 2 5 0 1 3
Vac/Vac Membrane 2 7 14 8 24 14 13 21 7 14 18 28 2 10 13 10 25 24 10 8 38 14 35 52
Unique Cell Count 166 137 323 280 348 423 650 695 561 678 554 719 158 267 294 127 189 177 166 140 337 116 242 240
Labelled Cell Count 207 162 345 351 421 591 982 980 838 1060 886 1083 160 272 306 139 213 190 166 140 337 116 242 240


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.7 4.7 4.3 4.3 3.7 3.6 3.6 3.5 3.4 3.2 3.3 6.0 5.8 5.6 6.6 6.6 6.8 5.6 6.3 6.7
Std Deviation (1e-4) 0.6 0.9 1.0 1.4 1.1 1.3 1.4 1.2 1.1 1.4 1.1 0.6 1.5 1.0 1.0 1.2 1.4 1.7 1.2 1.4 1.7
Intensity Change (Log2) – – – -0.13 -0.15 -0.35 -0.4 -0.41 -0.45 -0.48 -0.56 -0.52 0.33 0.28 0.24 0.48 0.49 0.51 0.25 0.41 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.5 3.7 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2192 1.9627 2.1665 2.375 1.64 1.9572 -0.0686 0.2438 -0.006 -0.0989 -0.493 0.0355 -0.1797 0.1656 -0.3027 0.321 -0.6197 -0.2256
Actin 0.0274 0.0001 0.0003 0.0002 0.0001 0 0.0011 0.0002 0.0005 0.0068 0 0.0002 0.0237 0.0014 0.0027 0.0027 0.0099 0.0002
Bud 0.0013 0.0001 0.0001 0 0 0 0.0003 0.0001 0.0001 0.0018 0 0.0002 0.0007 0.0001 0.0003 0.0005 0.0004 0
Bud Neck 0.0028 0.0004 0.0008 0.0002 0.0005 0.0018 0.0002 0.0001 0.0001 0.0007 0.0001 0.0015 0.0029 0.0001 0.0004 0.0027 0.0008 0.0008
Bud Periphery 0.0061 0 0.0001 0 0 0 0.0004 0.0001 0.0001 0.0012 0 0.0008 0.0018 0 0.0004 0.0014 0.001 0.0001
Bud Site 0.0019 0.0004 0.0001 0.0001 0.0001 0.0001 0.0006 0.0002 0.0001 0.0006 0 0.0001 0.0016 0.0001 0.003 0.0013 0.0015 0.0001
Cell Periphery 0.0035 0 0 0 0 0 0.0001 0 0 0.0001 0 0.0001 0.0005 0 0.0001 0.0008 0.0001 0
Cytoplasm 0.0175 0.042 0.0086 0.0154 0.0058 0.0066 0.0067 0.0104 0.0167 0.0333 0.0004 0.001 0.006 0.0051 0.0031 0.0015 0.0043 0.0017
Cytoplasmic Foci 0.0008 0.0002 0.0002 0.0001 0 0.0007 0.0577 0.0002 0.0096 0.0019 0 0 0.007 0.0016 0.0068 0.0016 0.0054 0.0001
Eisosomes 0.0004 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.012 0.0011 0.0004 0.0007 0.0007 0.0003 0.0033 0.0006 0.0005 0.0051 0 0.0005 0.0033 0.0009 0.0008 0.0022 0.0032 0.0002
Endosome 0.0094 0.0006 0.0005 0.0005 0.0005 0.0003 0.0277 0.0005 0.0085 0.0102 0 0.0014 0.0196 0.0011 0.0079 0.0204 0.0266 0.0026
Golgi 0.0017 0 0 0 0 0 0.0042 0 0.0011 0.0007 0 0 0.0054 0.0001 0.0019 0.0056 0.0053 0.0019
Lipid Particles 0.001 0 0.0001 0 0 0.0001 0.0119 0 0.0019 0.0002 0 0 0.021 0.0003 0.0061 0.0068 0.0303 0.0001
Mitochondria 0.0098 0.0003 0.001 0.0008 0.0004 0.0001 0.0044 0.0015 0.0162 0.0036 0.0002 0.003 0.0047 0.0008 0.0056 0.0687 0.0121 0.0068
None 0.0099 0.0035 0.0429 0.0196 0.0015 0.0157 0.0014 0.0015 0.0043 0.0012 0.0001 0.0001 0.0051 0.0042 0.0015 0.0006 0.0021 0.0001
Nuclear Periphery 0.037 0.0061 0.0092 0.0157 0.0271 0.0024 0.0315 0.0133 0.0129 0.0532 0.0036 0.0078 0.0137 0.0189 0.0129 0.0122 0.0304 0.0017
Nucleolus 0.0195 0.0043 0.0082 0.0071 0.0071 0.0097 0.0047 0.0032 0.0034 0.0019 0.0043 0.008 0.0183 0.0045 0.0065 0.0046 0.007 0.0075
Nucleus 0.8187 0.9372 0.9202 0.9247 0.9522 0.9353 0.8029 0.9537 0.918 0.8691 0.9909 0.9685 0.8404 0.9537 0.9151 0.8385 0.8358 0.9736
Peroxisomes 0.0005 0 0 0 0 0 0.0124 0 0.0009 0.0006 0 0 0.0068 0.0002 0.0183 0.0015 0.0081 0
Punctate Nuclear 0.0096 0.0026 0.0064 0.0141 0.0031 0.0261 0.018 0.0135 0.004 0.0023 0.0002 0.001 0.0092 0.0063 0.0052 0.002 0.0118 0.0019
Vacuole 0.006 0.001 0.0006 0.0005 0.0005 0.0004 0.0056 0.0005 0.0005 0.0031 0.0001 0.0029 0.0061 0.0003 0.001 0.0145 0.0017 0.0002
Vacuole Periphery 0.0034 0.0001 0.0004 0.0003 0.0003 0.0001 0.0049 0.0004 0.0005 0.0025 0.0001 0.0027 0.0022 0.0003 0.0004 0.0098 0.0019 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8519 2.2166 5.4826 6.4974 10.7162 3.3043 6.1632 4.2858 5.1323 7.6898
Translational Efficiency 1.3156 3.1565 1.1408 0.8871 0.6612 3.4245 1.6816 1.7348 1.5809 1.1351

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
938 321 1364 74 1720 2288 201 58 2658 2609 1565 132

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 666.64 835.63 833.58 925.63 738.77 771.13 930.76 968.44 713.32 779.07 846.06 944.44
Standard Deviation 122.70 95.84 105.51 97.04 94.37 114.60 103.98 154.87 110.74 114.44 110.22 127.55
Intensity Change Log 2 — 0.325956 0.322413 0.473528 — 0.061849 0.333284 0.390537 — 0.193163 0.328138 0.430499

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000669 0.000268 0.000264 0.000978 0.000043 0.000134 0.000608 0.000593 0.000264 0.000151 0.000308 0.000809
Bud Neck 0.016578 0.005564 0.005430 0.003102 0.002609 0.005052 0.002762 0.003478 0.007538 0.005115 0.005088 0.003267
Bud Site 0.015958 0.001657 0.000807 0.001197 0.000742 0.001493 0.001203 0.001439 0.006112 0.001513 0.000858 0.001303
Cell Periphery 0.000387 0.000084 0.000073 0.000116 0.000099 0.000135 0.000078 0.000128 0.000200 0.000128 0.000073 0.000121
Cytoplasm 0.046520 0.009593 0.011461 0.024288 0.033054 0.029826 0.007056 0.035167 0.037806 0.027337 0.010895 0.029068
Cytoplasmic Foci 0.019124 0.004975 0.000100 0.000644 0.002598 0.004800 0.000638 0.004787 0.008430 0.004822 0.000169 0.002464
Eisosomes 0.000065 0.000018 0.000013 0.000055 0.000008 0.000016 0.000030 0.000031 0.000028 0.000016 0.000016 0.000044
Endoplasmic Reticulum 0.012292 0.005287 0.004789 0.019969 0.001628 0.003020 0.007407 0.007985 0.005391 0.003299 0.005126 0.014704
Endosome 0.006203 0.002406 0.000256 0.001032 0.000270 0.001331 0.000590 0.007573 0.002364 0.001463 0.000299 0.003906
Golgi 0.003739 0.000283 0.000024 0.000073 0.000060 0.000443 0.000069 0.017804 0.001358 0.000424 0.000029 0.007864
Lipid Particles 0.007059 0.000470 0.000152 0.000796 0.000383 0.001725 0.000579 0.000474 0.002739 0.001570 0.000207 0.000655
Mitochondria 0.014861 0.001770 0.000679 0.000882 0.000650 0.003708 0.000636 0.001860 0.005665 0.003469 0.000674 0.001312
Mitotic Spindle 0.007400 0.000484 0.000997 0.000391 0.000128 0.005679 0.001433 0.022856 0.002694 0.005040 0.001053 0.010262
None 0.021471 0.002662 0.002684 0.002850 0.002570 0.004224 0.001669 0.003198 0.009240 0.004031 0.002554 0.003003
Nuclear Periphery 0.003238 0.000536 0.000730 0.001765 0.001200 0.001444 0.000698 0.001461 0.001919 0.001332 0.000726 0.001631
Nuclear Periphery Foci 0.007727 0.000179 0.000089 0.000789 0.000195 0.000568 0.000157 0.000558 0.002853 0.000520 0.000098 0.000688
Nucleolus 0.017244 0.003774 0.000993 0.002234 0.001358 0.004391 0.001311 0.000765 0.006964 0.004315 0.001034 0.001589
Nucleus 0.767845* 0.955786* 0.967769* 0.917298* 0.951032* 0.925958* 0.961804* 0.873794* 0.886386* 0.929628* 0.967003* 0.898182*
Peroxisomes 0.004931 0.000548 0.000056 0.000199 0.000182 0.001624 0.004059 0.000205 0.001858 0.001492 0.000570 0.000202
Vacuole 0.019893 0.002830 0.002488 0.021196 0.001125 0.003895 0.007086 0.013657 0.007748 0.003764 0.003078 0.017883
Vacuole Periphery 0.006795 0.000826 0.000145 0.000147 0.000067 0.000533 0.000125 0.002188 0.002441 0.000569 0.000142 0.001044

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.21 4.00 -3.63 -5.53 -5.87 -4.17 -4.28 -6.07 -5.33 -0.50 2.92 -2.43 -7.17 -8.22 -6.34
Bud Neck 4.64 4.86 5.91 0.90 1.86 -3.32 -0.91 -2.19 0.49 -0.85 2.46 1.14 3.10 0.70 1.87
Bud Site 6.06 7.17 6.43 0.06 -2.55 -1.46 -0.96 -1.89 -0.87 -0.95 5.40 6.28 4.63 -1.25 -2.99
Cell Periphery 6.62 7.52 5.75 -1.80 -3.09 -2.27 0.34 -1.15 0.41 -1.08 3.46 6.98 3.36 0.05 -3.44
Cytoplasm 7.84 8.46 2.87 -1.99 -1.94 0.83 7.99 -0.03 -0.18 -1.57 3.68 11.59 1.12 -0.04 -2.14
Cytoplasmic Foci 4.74 10.08 9.35 1.71 -1.74 -2.90 2.99 -0.11 1.33 -1.26 3.19 10.41 5.96 3.35 -1.98
Eisosomes 10.30 13.26 -1.25 -5.99 -6.60 -8.25 -7.13 -4.99 -4.02 -0.99 6.82 5.38 -5.67 -7.45 -7.12
Endoplasmic Reticulum 4.58 5.93 -3.62 -5.83 -6.13 -3.81 -7.73 -5.49 -4.77 -1.07 3.89 -1.14 -6.79 -7.91 -6.57
Endosome 2.49 7.88 5.70 0.65 -3.78 -3.90 -2.00 -1.29 -0.98 -1.17 2.33 7.42 -0.35 -0.95 -1.71
Golgi 3.73 4.05 3.93 1.03 -2.71 -3.43 -0.05 -1.07 -1.02 -1.06 2.73 4.08 -0.75 -0.98 -1.08
Lipid Particles 6.07 6.47 5.56 -2.40 -5.43 -4.01 -1.75 -1.56 3.10 0.17 2.35 6.53 4.64 2.33 -5.68
Mitochondria 6.60 7.96 7.76 0.72 -2.48 -4.03 -0.29 -1.19 2.34 -1.11 2.32 7.39 6.09 3.18 -1.70
Mitotic Spindle 3.18 3.62 0.53 -1.02 -1.16 -4.54 -1.20 -1.05 -0.60 -0.96 -2.06 2.00 -1.04 -0.59 -1.32
None 6.89 7.06 7.03 0.46 0.47 -2.23 1.81 -0.26 0.78 -1.02 4.69 6.45 5.39 1.67 -0.14
Nuclear Periphery 4.11 3.94 0.85 -5.76 -5.70 -1.58 1.95 -2.22 -1.71 -2.83 2.12 4.63 -1.65 -3.77 -5.88
Nuclear Periphery Foci 4.36 4.48 3.31 -2.02 -2.32 -1.67 -0.01 -2.08 -1.17 -2.12 3.67 4.54 1.95 -1.93 -3.13
Nucleolus 6.50 9.68 8.03 0.85 -1.77 -3.96 0.32 2.48 4.90 1.00 2.90 9.28 6.90 3.30 -1.31
Nucleus -13.87 -18.42 -5.85 3.78 5.01 5.44 0.19 2.91 2.10 2.80 -7.14 -15.27 0.93 3.29 5.39
Peroxisomes 6.57 7.65 7.36 1.88 -3.69 -4.12 -1.02 0.14 4.19 1.02 0.67 3.29 6.99 4.24 0.66
Vacuole 7.14 8.35 -2.55 -4.43 -4.56 -5.01 -3.84 -3.04 -2.86 -2.07 4.20 3.11 -4.44 -4.87 -4.78
Vacuole Periphery 4.85 5.76 5.70 1.39 -0.98 -2.73 -1.51 -1.47 -0.89 -1.37 4.24 5.55 3.21 -0.27 -1.46
Standard name
Human Ortholog
Description Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs
Localization
Cell Percentages nucleus (91%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp24

Prp24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available