Standard name
Human Ortholog
Description ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0.07 0 0.06 0.08 0.06 0.07 0.08 0.11 0.07 0 0 0 0.07 0.18 0.17 0.21 0.14 0.23 0.14 0.15 0.17
Bud 0 0 0 0 0.05 0 0 0.05 0.06 0.07 0.08 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0.07 0.06 0 0.06 0.09 0.07 0.08 0 0.07 0.09 0.1 0.14 0.15 0.06 0.08 0.12 0.11 0.1 0.14
Cytoplasm 0.91 0.93 0.9 0.86 0.84 0.75 0.67 0.73 0.53 0.5 0.44 0.46 0.93 0.96 0.96 0.8 0.69 0.69 0.18 0.34 0.14 0.34 0.28 0.21
Endoplasmic Reticulum 0 0 0 0.09 0 0.12 0.13 0.08 0.11 0.13 0.18 0.19 0 0 0 0.08 0.1 0.1 0.2 0.15 0.21 0.12 0.14 0.19
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.07 0.11 0.13 0.41 0.32 0.33 0.34 0 0 0 0 0 0 0.19 0.15 0.2 0.18 0.18 0.15
Nucleus 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 6 16 9 4 12 17 15 15 16 17 15 6 7 8 15 16 16 73 89 52 31 60 73
Bud 5 6 1 3 5 6 6 11 14 14 12 16 3 1 1 6 4 1 3 4 0 2 4 8
Bud Neck 0 13 7 0 2 1 2 1 0 0 1 0 2 3 5 1 1 0 17 29 8 6 14 15
Bud Site 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 19 13 5 2 15 13 9 13 18 11 16 10 25 34 20 12 14 21 55 27 24 40 63
Cytoplasm 265 543 190 111 92 154 146 173 118 104 69 93 225 345 382 162 61 64 63 221 30 75 113 92
Endoplasmic Reticulum 11 22 9 11 4 25 29 19 25 26 28 39 7 5 3 17 9 9 71 96 48 26 55 82
Endosome 1 0 0 0 1 0 1 1 0 1 0 1 0 0 0 1 3 1 8 11 2 4 5 5
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 1 1 1
Mitochondria 6 11 0 1 6 14 23 30 91 66 51 68 0 0 0 5 1 1 66 100 44 39 73 64
Nucleus 1 0 0 0 5 2 1 0 0 1 0 2 0 0 1 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 1 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 3 0 0 0 0 0 0 1 0 0 1 0 1 0 0 2
SpindlePole 0 1 1 0 2 0 3 8 1 5 1 2 1 2 2 12 3 5 0 3 0 1 6 3
Vac/Vac Membrane 0 0 0 0 0 2 3 5 0 0 0 0 0 2 1 0 0 0 17 26 4 4 17 15
Unique Cell Count 291 586 211 129 110 206 217 238 223 206 156 202 241 359 396 203 88 93 354 658 228 225 405 443
Labelled Cell Count 303 621 237 140 126 232 245 275 282 252 191 253 254 390 437 240 111 113 354 658 228 225 405 443


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.2 5.6 5.4 6.1 5.0 5.1 5.3 4.5 5.2 4.8 5.2 5.6 5.7 5.4 5.3 7.0 6.8 6.1 6.7 6.5
Std Deviation (1e-4) 0.7 0.7 1.2 1.0 5.5 0.8 1.0 0.9 0.9 1.0 0.9 1.0 1.1 1.4 1.5 1.2 2.0 1.1 1.6 1.9 1.8
Intensity Change (Log2) – – – -0.06 0.12 -0.17 -0.13 -0.09 -0.33 -0.12 -0.22 -0.11 0.0 0.03 -0.05 -0.07 0.32 0.28 0.12 0.24 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0938 1.4068 1.3627 1.1379 1.5314 1.0181 5.3321 4.691 4.7176 5.4087 4.745 4.4482 2.4368 2.2225 1.9832 1.6993 2.2457 1.8822
Actin 0.0271 0.006 0.0145 0.0143 0.2084 0.0233 0.0317 0.0146 0.0557 0.0188 0.0483 0.0535 0.0875 0.0104 0.0324 0.0025 0.0182 0.0142
Bud 0.0003 0.0003 0.0005 0.0001 0.002 0.0028 0.0036 0.0005 0.0043 0 0.0009 0.0003 0.0052 0.002 0.0026 0.0001 0.0005 0.0003
Bud Neck 0.008 0.0014 0.0027 0.0022 0.0018 0.0229 0.0048 0.0013 0.0048 0.0011 0.0467 0.003 0.0196 0.0009 0.006 0.0047 0.0023 0.013
Bud Periphery 0.0007 0.0009 0.0012 0.0005 0.0047 0.0022 0.0348 0.0015 0.0071 0.0002 0.0015 0.0007 0.0054 0.003 0.002 0.0009 0.0011 0.0013
Bud Site 0.0342 0.0192 0.0054 0.0004 0.0029 0.0014 0.0075 0.0434 0.0258 0.0002 0.006 0.0009 0.0232 0.024 0.0189 0.0003 0.0017 0.0004
Cell Periphery 0.023 0.0095 0.0078 0.0092 0.0015 0.0033 0.0105 0.014 0.0172 0.0019 0.0014 0.0027 0.0086 0.0105 0.0075 0.0049 0.003 0.0027
Cytoplasm 0.3192 0.344 0.3857 0.5919 0.2303 0.5608 0.1889 0.4342 0.3787 0.3176 0.2186 0.3375 0.2302 0.3921 0.3172 0.4006 0.3468 0.3751
Cytoplasmic Foci 0.1193 0.0131 0.0128 0.0312 0.064 0.0319 0.0163 0.0084 0.0248 0.0468 0.1019 0.0237 0.0435 0.0138 0.035 0.0037 0.0217 0.021
Eisosomes 0.0031 0.0024 0.001 0.0017 0.0015 0.0002 0.0028 0.0018 0.0012 0.0005 0.0009 0.0005 0.0022 0.001 0.001 0.0008 0.0004 0.0003
Endoplasmic Reticulum 0.019 0.0081 0.0162 0.0218 0.0075 0.0115 0.0458 0.0083 0.0146 0.002 0.0083 0.0254 0.0177 0.0229 0.012 0.0265 0.0057 0.0125
Endosome 0.0118 0.0046 0.0091 0.0055 0.0304 0.0119 0.0335 0.0015 0.0067 0.0008 0.0341 0.0088 0.0174 0.0029 0.0087 0.0012 0.0178 0.0065
Golgi 0.0083 0.0013 0.007 0.0026 0.0846 0.0139 0.0064 0.001 0.0117 0.0011 0.0284 0.0072 0.0156 0.0015 0.0086 0.0006 0.0191 0.0009
Lipid Particles 0.0755 0.0096 0.0079 0.0099 0.0172 0.0043 0.0214 0.0033 0.0059 0.0015 0.1049 0.0055 0.0271 0.0042 0.0071 0.0009 0.0179 0.0037
Mitochondria 0.003 0.0002 0.0246 0.0006 0.0323 0.0092 0.0133 0.0002 0.0209 0.0004 0.0216 0.0073 0.0102 0.0008 0.0026 0.0004 0.0201 0.0005
None 0.253 0.5749 0.4805 0.3038 0.2981 0.2907 0.4762 0.4597 0.4086 0.5942 0.3368 0.5123 0.4401 0.502 0.5207 0.548 0.4441 0.5388
Nuclear Periphery 0.0343 0.0005 0.0017 0.0005 0.0008 0.0008 0.0559 0.0006 0.0029 0.0001 0.0015 0.0007 0.0076 0.0008 0.0007 0.0004 0.0025 0.0003
Nucleolus 0.0013 0.0003 0.0002 0.0001 0.0002 0.0003 0.0021 0.0006 0.0004 0.0001 0.0003 0.0001 0.0025 0.0005 0.0015 0.0001 0.0007 0.0001
Nucleus 0.0046 0.0007 0.0008 0.0004 0.0003 0.0025 0.0199 0.001 0.0022 0.0003 0.0021 0.0003 0.0045 0.001 0.0014 0.0006 0.0015 0.0004
Peroxisomes 0.0465 0.0002 0.0125 0.0009 0.0065 0.0008 0.0016 0.0003 0.0018 0.0084 0.0312 0.0072 0.0193 0.0022 0.0085 0.0001 0.0448 0.0067
Punctate Nuclear 0.005 0.0006 0.0004 0.0006 0.0026 0.0027 0.0038 0.0012 0.0013 0.0008 0.003 0.001 0.0049 0.0015 0.002 0.0002 0.0285 0.0005
Vacuole 0.0018 0.0023 0.0066 0.0017 0.0012 0.0022 0.0062 0.0026 0.0028 0.0031 0.0009 0.0013 0.0054 0.002 0.0033 0.0023 0.0012 0.0007
Vacuole Periphery 0.0011 0.0001 0.0009 0.0001 0.001 0.0003 0.0131 0.0001 0.0005 0.0001 0.0007 0.0001 0.0023 0.0001 0.0004 0.0001 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8679 9.7021 11.199 7.638 9.3811 2.4105 9.5373 6.9887 9.7922 11.6684
Translational Efficiency 1.1442 1.0032 0.6632 1.0688 1.0178 6.8779 1.1751 1.5688 0.9322 0.8802

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1376 715 199 1747 1621 620 2376 326 2997 1335 2575 2073

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 686.70 787.55 907.12 898.62 717.62 849.62 873.35 963.37 703.42 816.38 875.96 908.80
Standard Deviation 107.85 145.22 108.61 123.10 88.09 115.06 118.12 142.07 98.87 135.65 117.76 128.45
Intensity Change Log 2 — 0.197692 0.401613 0.388031 — 0.243598 0.283340 0.424870 — 0.221332 0.342386 0.406974

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003883 0.014152 0.005500 0.007626 0.004691 0.002967 0.004154 0.031217 0.004320 0.008958 0.004258 0.011335
Bud Neck 0.025052 0.031029 0.005539 0.020540 0.029909 0.051368 0.016527 0.022650 0.027679 0.040475 0.015678 0.020872
Bud Site 0.005141 0.040442 0.015513 0.059200 0.010638 0.028017 0.022365 0.066851 0.008114 0.034672 0.021836 0.060403
Cell Periphery 0.001510 0.002535 0.002502 0.003464 0.003221 0.001555 0.003177 0.010548 0.002435 0.002080 0.003125 0.004578
Cytoplasm 0.379923* 0.348083* 0.312613* 0.375567* 0.485038* 0.429965* 0.389223* 0.217011* 0.436777* 0.386110* 0.383302* 0.350632*
Cytoplasmic Foci 0.254858* 0.221067* 0.120911 0.048251 0.173640 0.203697* 0.045300 0.068240 0.210929* 0.213000* 0.051143 0.051394
Eisosomes 0.008262 0.003399 0.000819 0.002517 0.013543 0.005320 0.001846 0.010664 0.011118 0.004291 0.001767 0.003798
Endoplasmic Reticulum 0.012429 0.014703 0.056636 0.043609 0.014648 0.005963 0.056328 0.106906* 0.013629 0.010644 0.056352 0.053563
Endosome 0.017023 0.019624 0.013819 0.005935 0.008600 0.019873 0.004230 0.010805 0.012467 0.019739 0.004971 0.006701
Golgi 0.024644 0.011941 0.001996 0.001372 0.022170 0.016432 0.001156 0.003518 0.023306 0.014027 0.001221 0.001710
Lipid Particles 0.079596 0.025346 0.036147 0.017556 0.036243 0.025760 0.019148 0.042505 0.056147 0.025538 0.020462 0.021480
Mitochondria 0.009204 0.007922 0.000449 0.001840 0.006025 0.009838 0.000843 0.000420 0.007485 0.008812 0.000813 0.001616
Mitotic Spindle 0.000261 0.002066 0.000591 0.010495 0.000230 0.003229 0.003063 0.007187 0.000244 0.002606 0.002872 0.009975
None 0.002280 0.016779 0.001766 0.001180 0.004101 0.002482 0.002665 0.000454 0.003265 0.010139 0.002595 0.001066
Nuclear Periphery 0.000760 0.000447 0.003537 0.001977 0.000295 0.000380 0.003190 0.001509 0.000509 0.000416 0.003217 0.001903
Nuclear Periphery Foci 0.000683 0.002851 0.024516 0.010011 0.000659 0.000492 0.009323 0.021256 0.000670 0.001756 0.010497 0.011780
Nucleolus 0.000791 0.000842 0.000830 0.000341 0.000486 0.001471 0.000481 0.000162 0.000626 0.001134 0.000508 0.000313
Nucleus 0.098188 0.099217 0.254945* 0.182923* 0.132490* 0.098122 0.328479* 0.082414 0.116741 0.098709 0.322797* 0.167117*
Peroxisomes 0.025478 0.035925 0.003071 0.002990 0.017872 0.016939 0.002492 0.006054 0.021364 0.027107 0.002537 0.003472
Vacuole 0.048980 0.099536 0.138035 0.201594* 0.034752 0.073113 0.085480 0.289472* 0.041284 0.087265 0.089541 0.215413*
Vacuole Periphery 0.001055 0.002094 0.000266 0.001015 0.000750 0.003015 0.000528 0.000155 0.000890 0.002522 0.000508 0.000880

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.18 -0.46 -3.30 3.51 -0.63 2.28 0.81 -4.67 -5.11 -4.84 -4.05 0.29 -5.25 -0.54 -5.19
Bud Neck -1.93 10.32 3.10 4.32 -9.23 -4.77 6.41 3.44 6.75 -1.24 -4.77 7.86 5.38 8.39 -2.57
Bud Site -9.70 -1.94 -13.30 -2.22 -5.93 -4.46 -5.31 -6.09 -3.67 -4.64 -9.91 -7.23 -13.80 -4.45 -8.59
Cell Periphery -1.98 -1.20 -5.37 -0.80 -0.82 5.64 0.58 -3.77 -5.07 -3.90 1.26 -1.93 -4.17 -4.32 -2.07
Cytoplasm 2.94 4.66 3.29 -0.41 -3.10 4.91 14.78 20.17 13.59 12.44 6.38 10.45 15.39 6.24 5.72
Cytoplasmic Foci 4.05 13.52 38.22 23.97 7.70 -3.68 28.45 16.78 15.83 -3.81 -0.25 42.02 43.26 30.71 0.75
Eisosomes 4.99 8.68 7.09 2.27 -4.44 7.27 12.79 3.04 -1.51 -3.81 9.12 14.50 11.59 2.36 -3.55
Endoplasmic Reticulum -0.99 -4.06 -8.70 -7.34 1.52 4.76 -12.27 -6.94 -7.84 -2.91 2.12 -13.70 -10.93 -11.79 2.47
Endosome -1.44 2.27 12.56 8.84 6.76 -5.39 6.22 -0.47 4.05 -3.11 -5.37 12.68 9.60 10.42 -2.24
Golgi 6.98 15.16 16.53 8.69 1.19 2.60 16.60 11.86 6.45 -1.32 6.61 23.13 22.74 11.38 -0.59
Lipid Particles 12.90 7.10 17.03 4.34 3.35 3.73 9.67 0.27 -2.09 -4.23 12.41 16.82 17.48 3.79 0.75
Mitochondria 0.92 8.95 7.51 4.92 -3.44 -2.78 7.44 8.01 8.10 3.97 -1.18 11.37 9.60 8.29 -2.15
Mitotic Spindle -2.03 -1.07 -5.94 -4.37 -5.72 -2.63 -4.20 -1.72 -0.84 -0.90 -3.33 -4.20 -6.19 -4.22 -4.09
None -3.50 0.74 1.79 3.85 1.26 1.35 1.60 3.98 2.69 5.11 -2.98 1.18 3.86 4.13 3.83
Nuclear Periphery 2.86 -5.17 -5.56 -7.32 2.57 -1.38 -15.11 -5.53 -5.03 5.09 1.50 -14.54 -7.78 -8.32 4.52
Nuclear Periphery Foci -3.61 -5.59 -14.88 -6.99 3.38 1.68 -13.30 -8.75 -8.82 -4.42 -3.37 -14.33 -17.09 -12.86 -0.47
Nucleolus -0.28 -0.09 2.81 2.81 3.61 -1.93 -0.38 3.52 2.50 3.88 -1.97 0.80 3.08 3.09 2.21
Nucleus -0.17 -9.43 -14.50 -11.65 5.08 5.59 -31.68 5.52 1.42 25.88 4.20 -38.19 -9.77 -11.94 25.61
Peroxisomes -2.97 14.94 15.78 10.74 0.73 0.49 13.72 9.36 7.10 -2.68 -2.76 20.59 19.89 13.24 -1.32
Vacuole -9.01 -9.37 -32.05 -18.10 -8.23 -7.40 -19.33 -20.18 -16.94 -15.47 -11.90 -19.82 -39.06 -26.13 -24.91
Vacuole Periphery -2.28 11.10 0.82 2.36 -1.65 -1.99 0.89 6.19 2.53 1.14 -2.78 1.56 0.74 2.86 -0.59
Standard name
Human Ortholog
Description ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol
Localization
Cell Percentages ER (36%), cytoplasm (1%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gpi12

Gpi12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpi12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available