Standard name
Human Ortholog
Description Initiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.98 0.96 0.89 0.82 0.78 0.65 0.65 0.53 0.56 1.0 0.99 1.0 0.98 0.98 0.95 0.95 0.94 0.93 0.91 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.11 0.07 0.35 0.4 0.41 0.55 0.6 0.7 0.67 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 1 0 0 0 1 0 2 2
Bud 4 1 1 0 1 6 7 4 5 4 7 10 0 0 1 1 0 0 0 0 1 0 6 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 2
Bud Site 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 10 1 2 4 1 7 2 4 8 3 9 5 8 12 6 3 13 0 0 0 0 0 0
Cytoplasm 216 429 242 170 242 317 439 319 191 275 173 243 384 394 512 240 144 140 217 422 232 102 296 242
Endoplasmic Reticulum 0 3 1 0 1 0 0 2 5 2 2 0 0 2 1 14 5 8 1 1 2 0 2 4
Endosome 0 1 0 1 1 2 2 4 0 1 0 0 0 1 0 0 0 0 1 5 0 1 1 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 1
Mitochondria 17 6 2 20 17 125 212 166 161 256 227 288 0 1 0 9 3 3 2 5 5 2 4 6
Nucleus 0 0 0 1 0 3 0 0 0 1 1 1 0 0 1 0 2 0 0 0 0 0 1 0
Nuclear Periphery 2 0 0 0 0 0 2 1 0 1 0 1 1 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 4 4 1 1 3 5 0 1 0 0 0 0 0 1 0 0 1 1
Vac/Vac Membrane 0 3 2 0 3 2 8 2 0 2 5 1 0 1 0 2 1 1 1 6 1 1 2 6
Unique Cell Count 223 439 245 174 252 358 534 407 294 426 324 432 385 399 514 246 147 147 228 447 250 113 329 275
Labelled Cell Count 239 453 250 194 269 456 681 507 369 551 422 559 391 408 528 274 159 166 228 447 250 113 329 275


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.5 4.8 4.4 4.5 3.8 3.5 3.9 3.4 3.2 3.2 3.2 5.6 5.5 5.3 6.6 7.5 7.7 5.3 5.7 6.5
Std Deviation (1e-4) 0.5 0.7 0.9 1.3 1.1 1.0 1.0 1.6 0.6 0.9 0.9 1.3 1.1 1.3 1.1 1.1 1.2 1.3 1.3 1.7 1.6
Intensity Change (Log2) – – – -0.11 -0.08 -0.32 -0.45 -0.29 -0.49 -0.56 -0.56 -0.58 0.23 0.2 0.16 0.47 0.66 0.69 0.17 0.27 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.4
Cytoplasm -0.8 -1.9 -4.7 -6.5 -7.3 -9.8 -10.1 -12.1 -11.8 1.5 0 1.3 -1.0 -0.6 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7223 0.9018 0.6984 0.7576 0.075 0.5399 2.3851 2.5909 2.2788 1.9725 2.6809 2.418 5.0463 3.5458 4.1477 2.5359 2.5408 2.9492
Actin 0.2813 0.0002 0.0107 0.091 0.0003 0.014 0.0551 0.0213 0.0082 0.0118 0.0095 0.0083 0.0584 0.0043 0.0099 0.0005 0.0141 0.018
Bud 0.0016 0.0001 0.0005 0.0009 0.0002 0.0001 0.0024 0.0063 0.0027 0.0043 0.0029 0.0014 0.0115 0.0003 0.03 0.0001 0.0022 0.0004
Bud Neck 0.006 0.0001 0.0006 0.0015 0.0002 0.0027 0.002 0.0003 0.0005 0.0005 0.0002 0.0035 0.0027 0.0004 0.0008 0.0001 0.0023 0.001
Bud Periphery 0.0023 0 0.0007 0.0011 0.0001 0.0002 0.0038 0.008 0.0037 0.0142 0.0107 0.0028 0.0572 0.0003 0.0088 0.0001 0.0035 0.0004
Bud Site 0.0467 0.0004 0.0029 0.0041 0.0001 0.0001 0.0136 0.0207 0.0113 0.0024 0.0008 0.0002 0.0058 0.0113 0.0171 0.0003 0.0012 0.0004
Cell Periphery 0.0014 0.0004 0.0005 0.0003 0.0001 0.0002 0.0007 0.0005 0.0003 0.0003 0.0003 0.0002 0.0014 0.0004 0.0008 0.0002 0.0001 0.0001
Cytoplasm 0.2976 0.5694 0.564 0.363 0.7494 0.5758 0.2087 0.3828 0.3159 0.4051 0.2651 0.3796 0.1752 0.4903 0.2463 0.3793 0.5525 0.4621
Cytoplasmic Foci 0.0233 0.0042 0.0184 0.013 0.0098 0.0064 0.038 0.0261 0.0179 0.0312 0.0117 0.0154 0.0785 0.0167 0.0252 0.0094 0.0078 0.0222
Eisosomes 0.0038 0.0001 0.0002 0.0005 0 0.0009 0.0008 0.0003 0.0002 0.0002 0.0004 0.0001 0.0007 0.0003 0.0007 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0352 0.0049 0.0081 0.0026 0.0026 0.0051 0.0099 0.0018 0.0031 0.0023 0.0042 0.0017 0.0044 0.0058 0.0035 0.0038 0.0034 0.0022
Endosome 0.0076 0.0024 0.0224 0.0137 0.0033 0.004 0.0416 0.0047 0.0219 0.0236 0.0144 0.0151 0.0138 0.0063 0.014 0.0048 0.0056 0.0031
Golgi 0.0163 0.0002 0.0076 0.0208 0.0003 0.0014 0.0132 0.0041 0.019 0.0138 0.0044 0.0022 0.0059 0.0016 0.0052 0.0002 0.0006 0.0014
Lipid Particles 0.0145 0.0005 0.0202 0.0161 0.0001 0.004 0.0204 0.0007 0.0095 0.0057 0.0011 0.0013 0.017 0.0036 0.0066 0.0009 0.0003 0.001
Mitochondria 0.0048 0.0001 0.0375 0.0818 0.0001 0.0007 0.0112 0.0289 0.0168 0.0085 0.1651 0.0013 0.0015 0.001 0.0069 0.0002 0.0016 0.001
None 0.2352 0.4124 0.2756 0.3752 0.2314 0.3734 0.5193 0.4817 0.5489 0.4563 0.4517 0.5505 0.4896 0.4455 0.608 0.5947 0.3643 0.4832
Nuclear Periphery 0.0033 0.0009 0.0012 0.001 0.0003 0.0029 0.0117 0.0005 0.001 0.0027 0.0386 0.0072 0.0028 0.0008 0.0017 0.001 0.0084 0.0005
Nucleolus 0.0007 0.0001 0.0002 0.0002 0 0.0004 0.001 0.0001 0.0002 0.0003 0.0003 0.0001 0.0012 0.0003 0.0012 0.0001 0.0014 0.0001
Nucleus 0.0013 0.0009 0.0009 0.0006 0.0004 0.0018 0.0188 0.0007 0.0008 0.0011 0.0071 0.0005 0.004 0.0011 0.0019 0.0009 0.0207 0.0003
Peroxisomes 0.0105 0 0.0148 0.0092 0 0.0016 0.0159 0.0084 0.0147 0.0101 0.0015 0.0059 0.046 0.0061 0.0053 0.0001 0.0005 0.0009
Punctate Nuclear 0.0051 0.0004 0.0006 0.0011 0.0002 0.0032 0.0043 0.0007 0.001 0.0027 0.0014 0.0004 0.0206 0.0018 0.0033 0.0006 0.0074 0.0008
Vacuole 0.001 0.0022 0.0107 0.0016 0.001 0.001 0.0056 0.0012 0.0019 0.0018 0.0035 0.0013 0.0013 0.0015 0.0022 0.0022 0.0015 0.0007
Vacuole Periphery 0.0004 0.0002 0.0016 0.0006 0.0001 0.0003 0.0021 0.0003 0.0007 0.001 0.0052 0.0009 0.0003 0.0002 0.0006 0.0003 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7979 8.3158 7.5946 8.0359 5.5666 7.1517 8.2464 9.3854 7.9752 8.788
Translational Efficiency 0.9181 0.5686 0.7611 0.5701 0.5238 0.7567 0.7714 0.5901 0.6953 0.5715

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2298 1702 1431 2327 1747 135 2030 1231 4045 1837 3461 3558

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 731.23 749.99 898.60 878.11 745.65 848.36 839.07 879.46 737.46 757.22 863.68 878.58
Standard Deviation 82.06 121.81 112.55 108.15 120.45 99.79 110.38 128.99 100.71 123.04 115.08 115.79
Intensity Change Log 2 — 0.036546 0.297354 0.264076 — 0.186178 0.170293 0.238119 — 0.114031 0.234602 0.251030

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000404 0.000220 0.001930 0.000407 0.001476 0.000067 0.001286 0.000222 0.000483 0.000130 0.001707
Bud Neck 0.013852 0.025972 0.002814 0.007411 0.015029 0.018464 0.002610 0.009471 0.014361 0.025420 0.002694 0.008124
Bud Site 0.008272 0.030028 0.009989 0.033330 0.011383 0.033906 0.003922 0.066965 0.009616 0.030313 0.006430 0.044967
Cell Periphery 0.000142 0.000122 0.000108 0.000132 0.001447 0.000172 0.000168 0.000131 0.000706 0.000126 0.000143 0.000132
Cytoplasm 0.587788* 0.495423* 0.577355* 0.638691* 0.565473* 0.334782* 0.629244* 0.505909* 0.578150* 0.483618* 0.607790* 0.592751*
Cytoplasmic Foci 0.165957 0.206004* 0.018814 0.013452 0.192147* 0.271207* 0.029823 0.026664 0.177269 0.210796* 0.025271 0.018023
Eisosomes 0.000102 0.000089 0.000035 0.000020 0.002829 0.000172 0.000057 0.000033 0.001280 0.000095 0.000048 0.000025
Endoplasmic Reticulum 0.001279 0.000817 0.001905 0.000512 0.001550 0.000727 0.000394 0.001159 0.001396 0.000810 0.001019 0.000735
Endosome 0.018378 0.034059 0.003367 0.004049 0.018650 0.067230 0.003847 0.023527 0.018495 0.036497 0.003648 0.010788
Golgi 0.003508 0.008158 0.000127 0.002201 0.004610 0.012141 0.000284 0.018417 0.003984 0.008451 0.000219 0.007811
Lipid Particles 0.004346 0.003412 0.001210 0.000540 0.005679 0.004504 0.001438 0.001201 0.004922 0.003493 0.001344 0.000769
Mitochondria 0.001704 0.004760 0.000664 0.002761 0.003577 0.002764 0.000300 0.008147 0.002513 0.004613 0.000451 0.004624
Mitotic Spindle 0.000201 0.002541 0.005547 0.076096 0.002215 0.006944 0.004888 0.089842 0.001071 0.002865 0.005161 0.080852
None 0.003343 0.002835 0.005244 0.004943 0.018316 0.006273 0.006906 0.005073 0.009810 0.003088 0.006219 0.004988
Nuclear Periphery 0.000170 0.000232 0.001343 0.000801 0.000444 0.000150 0.000488 0.001078 0.000289 0.000226 0.000841 0.000897
Nuclear Periphery Foci 0.000312 0.000357 0.001904 0.000410 0.000617 0.000845 0.000519 0.001161 0.000444 0.000393 0.001091 0.000670
Nucleolus 0.000763 0.001220 0.000327 0.000263 0.000513 0.000474 0.000476 0.000933 0.000655 0.001165 0.000414 0.000495
Nucleus 0.056032 0.033700 0.264788* 0.127960 0.044762 0.017904 0.198837* 0.061521 0.051165 0.032539 0.226105* 0.104973
Peroxisomes 0.001560 0.007499 0.000830 0.002730 0.006654 0.019707 0.000257 0.002478 0.003760 0.008396 0.000494 0.002643
Vacuole 0.129913 0.138617 0.103247 0.080640 0.101605 0.197103 0.114846 0.153928 0.117687 0.142915 0.110050 0.105996
Vacuole Periphery 0.002293 0.003750 0.000163 0.001128 0.002092 0.003055 0.000630 0.021077 0.002206 0.003699 0.000437 0.008030

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.25 -7.69 -3.89 -1.75 -2.59 -1.91 1.54 -0.52 1.79 -4.17 -2.22 0.66 -2.89 -1.27 -4.35
Bud Neck -6.86 10.66 8.61 12.51 -3.52 -0.94 13.38 8.38 4.17 -4.77 -6.96 17.31 12.08 13.10 -6.52
Bud Site -11.63 0.69 -4.25 6.71 -3.82 -3.70 6.59 -5.96 0.42 -8.76 -11.46 5.42 -7.00 4.58 -9.74
Cell Periphery 1.79 5.30 6.68 2.64 2.37 4.61 4.75 4.95 2.75 4.87 4.86 4.87 5.22 3.19 5.18
Cytoplasm 10.92 13.81 7.66 -3.82 -6.96 9.23 -2.33 9.23 -5.08 11.41 12.15 6.87 11.27 -2.94 4.01
Cytoplasmic Foci -6.73 43.73 46.34 41.27 4.42 -4.08 36.50 39.53 14.51 8.78 -5.98 55.70 60.75 43.31 11.69
Eisosomes 2.47 14.16 20.46 13.12 9.25 4.71 4.93 4.96 4.44 7.05 4.86 5.04 5.12 13.11 12.68
Endoplasmic Reticulum 2.60 -9.21 3.09 0.03 15.34 2.61 3.86 1.77 -1.67 -5.54 3.48 -1.76 3.44 -0.87 9.67
Endosome -8.38 17.36 18.74 19.59 4.60 -4.38 14.36 8.03 5.49 -3.35 -9.64 21.92 19.69 18.69 -0.48
Golgi -7.03 16.76 9.78 11.52 -2.35 -2.09 13.93 -0.28 1.87 -4.02 -6.73 21.02 4.02 8.22 -4.48
Lipid Particles 2.79 11.14 14.28 10.42 8.60 1.05 7.13 9.19 3.57 0.91 4.16 10.65 15.63 10.66 2.42
Mitochondria -4.26 5.85 2.16 4.86 -3.12 0.81 4.12 1.06 0.00 -4.59 -2.74 5.90 1.97 4.19 -6.13
Mitotic Spindle -3.87 -4.41 -12.24 -11.09 -9.43 -1.17 -1.68 -9.72 -6.39 -9.02 -2.67 -4.47 -15.51 -14.24 -13.20
None 0.78 -1.71 -7.36 -5.08 -5.03 2.09 4.57 4.67 0.10 0.18 4.88 3.80 3.04 -3.97 -2.17
Nuclear Periphery -1.06 -23.48 -15.99 -13.74 1.41 1.00 -0.87 -3.87 -14.09 -9.08 0.43 -8.17 -10.88 -16.39 -6.02
Nuclear Periphery Foci -1.78 -7.07 -5.90 -3.44 6.22 -1.01 0.29 -0.91 0.48 -2.30 0.64 -6.26 -2.44 -4.67 5.44
Nucleolus -2.22 3.59 3.10 4.41 -1.44 0.04 1.62 -0.48 -0.37 -1.36 -2.87 3.73 2.46 4.05 -1.51
Nucleus 9.48 -37.41 -33.27 -37.81 16.26 6.07 -32.63 -18.30 -19.20 9.81 9.10 -47.61 -37.92 -40.73 15.10
Peroxisomes -6.78 3.80 2.90 7.51 -1.03 -2.69 5.42 4.91 4.14 -3.23 -4.53 6.45 5.46 8.58 -2.71
Vacuole -2.56 -6.14 -11.52 -8.49 -3.43 -5.50 -12.14 -20.41 -3.41 -11.41 -6.53 -12.21 -22.06 -13.10 -9.53
Vacuole Periphery -4.75 15.06 10.98 12.19 -2.05 -0.75 4.74 -2.98 -1.82 -4.31 -5.05 9.77 -0.00 2.90 -4.24
Standard name
Human Ortholog
Description Initiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rit1

Rit1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rit1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available