Standard name
Human Ortholog
Description 3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0.09 0 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.05 0.14 0.18 0.19 0.09 0.09 0.07 0.1 0.06 0.06 0 0 0.21 0.36 0.38 0.32 0.17 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.21 0 0 0 0.11 0 0 0 0 0 0 0 0.09 0.13 0.06 0.22 0.23 0.31 0.11 0.1 0.11 0.07 0.08 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05
Mitochondria 0.58 0.89 0.78 0.8 0.79 0.92 0.91 0.9 0.91 0.94 0.95 0.93 0.7 0.47 0.57 0.19 0.09 0.09 0.71 0.68 0.69 0.72 0.66 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0 0 0 0.13 0 0 0 0 0 0 0 0.05 0.1 0 0.05 0.09 0.19 0.05 0 0.05 0.05 0.05 0.08
SpindlePole 0.08 0 0.08 0 0.05 0 0 0.07 0 0.07 0.05 0.07 0 0 0 0.14 0.25 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.09 0 0 0.07 0.08 0 0.05 0 0 0 0 0.07 0.09 0.12 0.16 0.17 0.12 0 0.06 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 3 0 2 0 0 0 0 0 0 0 1 0 0 1 0 0 2 4 9 3 5 16
Bud 3 3 8 6 7 12 18 15 19 17 22 39 2 5 19 0 2 2 0 0 0 1 2 1
Bud Neck 1 0 2 0 0 0 0 1 0 0 2 0 0 0 0 0 3 2 1 0 2 3 1 3
Bud Site 0 0 2 1 2 3 5 17 7 9 11 13 0 1 0 0 0 0 – – – – – –
Cell Periphery 4 4 3 7 2 3 1 2 1 4 6 4 3 2 2 0 4 0 0 0 0 0 0 1
Cytoplasm 7 18 35 35 20 30 28 43 23 17 16 18 35 98 113 12 14 15 1 1 1 3 3 2
Endoplasmic Reticulum 1 4 2 2 1 4 1 1 4 0 1 2 3 5 0 0 5 2 0 1 2 1 3 0
Endosome 30 0 2 4 24 5 7 6 0 0 1 0 15 36 19 8 18 18 20 13 25 14 21 33
Golgi 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 7 4 1 4 7 12
Mitochondria 81 116 153 152 170 304 384 396 333 274 355 402 117 126 173 7 7 5 135 94 158 139 180 152
Nucleus 1 1 1 0 0 4 5 19 7 6 3 6 1 0 0 1 1 2 0 0 0 1 3 0
Nuclear Periphery 2 2 0 0 0 4 5 4 3 1 6 5 0 0 0 0 1 0 1 1 1 0 1 2
Nucleolus 0 0 0 0 0 6 1 5 5 2 7 3 0 0 1 0 0 0 1 0 0 2 2 3
Peroxisomes 11 1 3 8 27 3 17 5 0 6 6 4 8 27 13 2 7 11 9 4 12 9 13 21
SpindlePole 11 1 16 6 10 12 19 33 16 19 19 30 3 9 4 5 20 11 2 1 3 1 7 3
Vac/Vac Membrane 20 12 4 3 15 28 16 23 11 6 11 6 11 23 36 6 14 7 1 7 4 6 16 10
Unique Cell Count 140 130 195 189 214 331 423 441 366 292 374 434 167 269 301 37 80 58 190 139 229 194 273 270
Labelled Cell Count 176 162 234 224 280 418 507 570 429 361 466 532 199 332 380 44 98 75 190 139 229 194 273 270


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 3.7 3.7 4.2 4.2 3.7 3.5 3.6 3.3 3.1 3.3 3.1 4.4 4.7 4.2 5.8 6.2 6.1 5.0 4.7 5.4
Std Deviation (1e-4) 0.7 0.6 1.1 2.3 1.5 1.2 1.2 1.2 1.4 1.0 1.1 1.2 1.1 1.6 1.6 1.5 1.8 2.2 1.5 1.2 1.6
Intensity Change (Log2) – – – 0.21 0.2 0.0 -0.05 -0.04 -0.15 -0.23 -0.16 -0.23 0.28 0.36 0.2 0.66 0.76 0.73 0.44 0.36 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 4.7 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6217 1.5809 1.7151 1.6416 1.5207 1.5854 1.8323 2.1308 2.0482 1.2822 1.8605 2.152 3.3568 2.9012 3.2019 2.8431 3.3566 3.2984
Actin 0.0227 0.0146 0.0223 0.0034 0.0085 0.017 0.0233 0.0286 0.0347 0.0748 0.0514 0.0167 0.0587 0.0111 0.0324 0.0043 0.0208 0.0281
Bud 0.0008 0.0014 0.0028 0.0006 0.0012 0.0014 0.0007 0.0036 0.0009 0.0025 0.0026 0.0005 0.0018 0.0012 0.0021 0.0005 0.0015 0.0013
Bud Neck 0.0009 0.0008 0.003 0.0004 0.0006 0.009 0.0008 0.0028 0.0026 0.0018 0.0014 0.003 0.0033 0.0008 0.0028 0.0013 0.0009 0.002
Bud Periphery 0.0024 0.002 0.0055 0.0022 0.0036 0.007 0.0013 0.0059 0.0021 0.0074 0.0033 0.0013 0.0041 0.0019 0.0051 0.003 0.0078 0.005
Bud Site 0.0027 0.019 0.0206 0.0008 0.0011 0.0044 0.0023 0.042 0.0088 0.0113 0.0036 0.0009 0.03 0.0109 0.0141 0.0008 0.002 0.0012
Cell Periphery 0.0009 0.0006 0.0026 0.0003 0.0003 0.001 0.0005 0.0014 0.002 0.0139 0.0004 0.0006 0.0018 0.0004 0.0015 0.0062 0.0035 0.0016
Cytoplasm 0.0048 0.0122 0.0056 0.0006 0.0007 0.0055 0.0066 0.021 0.0062 0.0012 0.0169 0.0083 0.0116 0.0037 0.0062 0.0162 0.007 0.0204
Cytoplasmic Foci 0.0354 0.0481 0.0447 0.0512 0.0399 0.0397 0.077 0.0955 0.075 0.02 0.117 0.0629 0.0566 0.064 0.0662 0.0184 0.089 0.0449
Eisosomes 0.0006 0.0006 0.001 0.0003 0.0002 0.0004 0.0005 0.0008 0.0027 0.0024 0.0002 0.0006 0.0017 0.0004 0.0014 0.0053 0.0015 0.0013
Endoplasmic Reticulum 0.0044 0.0013 0.0011 0.0013 0.0005 0.0012 0.0028 0.0015 0.0033 0.0014 0.0039 0.0017 0.0028 0.0006 0.0006 0.0004 0.0014 0.0049
Endosome 0.1006 0.1058 0.0546 0.0508 0.0988 0.0439 0.1255 0.1023 0.089 0.0346 0.2087 0.0838 0.0726 0.0606 0.0477 0.0765 0.0489 0.0438
Golgi 0.0744 0.1009 0.0628 0.0277 0.1191 0.0569 0.0863 0.0969 0.0458 0.0417 0.1812 0.0754 0.0645 0.0921 0.0577 0.0253 0.078 0.0701
Lipid Particles 0.0679 0.0148 0.0214 0.0203 0.0251 0.0328 0.092 0.0183 0.0295 0.0054 0.0154 0.0485 0.0597 0.0248 0.0204 0.0038 0.0198 0.0212
Mitochondria 0.5789 0.5886 0.6668 0.7579 0.6359 0.7078 0.4414 0.4141 0.5296 0.7612 0.2861 0.6182 0.5511 0.6007 0.6344 0.8094 0.596 0.6188
None 0.018 0.0163 0.0183 0.0006 0.0003 0.0078 0.0058 0.0283 0.0363 0.0008 0.0012 0.0088 0.0199 0.0009 0.0107 0.0164 0.0048 0.0549
Nuclear Periphery 0.0105 0.0003 0.0005 0.0056 0.0004 0.002 0.0111 0.0005 0.0046 0.0003 0.0006 0.0042 0.001 0.0001 0.0002 0.0001 0.0001 0.0008
Nucleolus 0.0013 0.0003 0.0007 0.0004 0.0002 0.0007 0.0009 0.0006 0.0014 0.0002 0.0002 0.0003 0.0004 0.0002 0.0017 0.0002 0.0002 0.0003
Nucleus 0.0017 0.0003 0.0005 0.0009 0.0003 0.0005 0.0038 0.0006 0.0006 0.0003 0.0004 0.0004 0.0006 0.0002 0.0005 0.0002 0.0002 0.0004
Peroxisomes 0.0522 0.0602 0.0581 0.0644 0.0573 0.0518 0.0947 0.1236 0.1138 0.0142 0.0898 0.0563 0.0508 0.1209 0.0892 0.0085 0.1137 0.0721
Punctate Nuclear 0.0045 0.0005 0.0007 0.0003 0.0003 0.0025 0.0035 0.0005 0.0012 0.0003 0.0004 0.001 0.0008 0.0002 0.001 0 0.0003 0.0012
Vacuole 0.0086 0.0072 0.0041 0.0038 0.0031 0.0034 0.0109 0.0087 0.005 0.0019 0.007 0.0035 0.0041 0.0023 0.0026 0.0016 0.0015 0.0034
Vacuole Periphery 0.0056 0.0042 0.0024 0.0059 0.0028 0.0032 0.0082 0.0026 0.0047 0.0023 0.0084 0.0031 0.0021 0.0017 0.0017 0.0016 0.0012 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.2898 6.4403 8.0488 8.9252 8.8491 7.9583 9.7676 11.3344 10.6859 12.8614
Translational Efficiency 0.3737 0.5201 0.4581 0.3323 0.3123 0.3981 0.4167 0.3135 0.3779 0.2982

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1780 1706 132 1299 1806 1841 144 136 3586 3547 276 1435

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 617.46 747.05 1057.17 866.99 698.07 743.71 1003.74 952.61 658.06 745.32 1029.29 875.10
Standard Deviation 65.50 99.76 120.41 106.36 85.22 94.51 103.99 113.90 86.09 97.09 115.28 110.00
Intensity Change Log 2 — 0.274859 0.775790 0.489670 — 0.091368 0.523942 0.448514 — 0.180404 0.647637 0.467977

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000452 0.001644 0.012932 0.017642 0.000716 0.000629 0.017616 0.056294 0.000585 0.001117 0.015376 0.021305
Bud Neck 0.053642 0.014432 0.014446 0.019790 0.009705 0.013563 0.017750 0.021851 0.031515 0.013981 0.016170 0.019986
Bud Site 0.021636 0.021798 0.062250 0.101485* 0.009659 0.016664 0.059721 0.162399* 0.015604 0.019133 0.060931 0.107258*
Cell Periphery 0.000336 0.000206 0.000302 0.000355 0.000218 0.000184 0.000471 0.000414 0.000277 0.000195 0.000390 0.000361
Cytoplasm 0.068910 0.024692 0.059376 0.060599 0.015463 0.018151 0.080634 0.065384 0.041993 0.021297 0.070467 0.061053
Cytoplasmic Foci 0.186538* 0.132722 0.181668* 0.175258 0.112413 0.124411 0.132964 0.173100 0.149207 0.128409 0.156257 0.175053
Eisosomes 0.000211 0.000109 0.000176 0.000189 0.000112 0.000121 0.000265 0.000250 0.000161 0.000115 0.000222 0.000195
Endoplasmic Reticulum 0.001472 0.000551 0.015907 0.002880 0.000724 0.000569 0.018397 0.002357 0.001095 0.000561 0.017206 0.002830
Endosome 0.109412 0.125601 0.087458 0.079208 0.094292 0.130922 0.064273 0.055041 0.101797 0.128363 0.075361 0.076917
Golgi 0.059526 0.138027* 0.043235 0.075103 0.152076* 0.141978* 0.016878 0.037519 0.106137 0.140078* 0.029483 0.071541
Lipid Particles 0.028868 0.006454 0.015124 0.003607 0.015211 0.007621 0.016233 0.002595 0.021990 0.007060 0.015703 0.003511
Mitochondria 0.330385* 0.383585* 0.008673 0.095700 0.504189* 0.422691* 0.005291 0.021252 0.417917* 0.403882* 0.006908 0.088644
Mitotic Spindle 0.007028 0.026704 0.174479* 0.098052 0.008891 0.013893 0.099658 0.055415 0.007966 0.020055 0.135442* 0.094011
None 0.003030 0.000760 0.000574 0.000204 0.001058 0.000400 0.001801 0.000061 0.002037 0.000573 0.001214 0.000191
Nuclear Periphery 0.000419 0.000167 0.002182 0.000315 0.000438 0.000224 0.005724 0.000109 0.000429 0.000196 0.004030 0.000295
Nuclear Periphery Foci 0.000340 0.000366 0.006949 0.000795 0.000335 0.000204 0.008989 0.000689 0.000338 0.000282 0.008013 0.000785
Nucleolus 0.003904 0.001786 0.004502 0.000851 0.001480 0.001164 0.006723 0.000163 0.002683 0.001463 0.005661 0.000786
Nucleus 0.016347 0.003770 0.050302 0.008603 0.003790 0.002873 0.144190* 0.003789 0.010023 0.003304 0.099287 0.008147
Peroxisomes 0.048491 0.067475 0.076936 0.148178* 0.039077 0.055683 0.103751* 0.258888* 0.043750 0.061355 0.090926 0.158671*
Vacuole 0.042010 0.031149 0.161538 0.102093 0.015588 0.030597 0.194170 0.079290 0.028703 0.030862 0.178563 0.099932
Vacuole Periphery 0.017042 0.018003 0.020991 0.009092 0.014564 0.017457 0.004503 0.003138 0.015794 0.017719 0.012389 0.008528

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.55 -4.56 -10.07 -9.16 -1.22 0.27 -4.95 -5.31 -5.32 -3.51 -2.32 -6.72 -11.13 -10.83 -2.03
Bud Neck 12.40 10.74 10.27 -3.42 -2.25 -2.41 -2.50 -2.47 -1.58 -0.55 9.65 6.05 5.21 -4.34 -1.87
Bud Site -0.12 -4.74 -17.75 -17.48 -3.99 -3.77 -4.99 -8.86 -8.43 -5.61 -2.57 -6.85 -21.24 -20.23 -6.55
Cell Periphery 2.60 0.58 -0.00 -1.20 -0.25 1.34 -4.21 -1.56 -1.92 0.93 2.98 -3.12 -0.68 -1.54 0.64
Cytoplasm 11.04 0.30 -0.03 -11.13 -0.31 -1.31 -6.09 -4.86 -4.64 -0.00 8.96 -4.01 -7.35 -13.68 0.36
Cytoplasmic Foci 9.46 1.31 3.15 -6.08 0.09 -2.62 0.04 -3.55 -2.73 -2.91 5.65 1.13 -3.49 -7.97 -2.89
Eisosomes 6.73 1.51 1.48 -5.65 -0.28 -0.83 -6.70 -3.39 -3.08 0.86 4.94 -4.30 -2.42 -6.22 2.19
Endoplasmic Reticulum 2.98 -5.43 -4.27 -9.17 4.83 1.06 -8.52 -4.59 -5.10 7.76 3.14 -9.81 -6.77 -9.78 8.71
Endosome -3.02 3.24 7.54 10.67 1.08 -6.78 4.87 5.63 9.93 0.63 -6.97 5.76 8.07 14.48 -0.50
Golgi -15.93 2.76 -1.75 12.90 -3.59 1.63 23.39 15.81 15.01 -2.74 -8.31 16.61 9.86 17.26 -7.49
Lipid Particles 10.11 4.30 12.70 2.43 4.36 4.47 -0.54 8.21 4.29 7.51 10.69 3.23 14.77 4.34 7.80
Mitochondria -4.70 40.51* 26.37 30.68* -14.75 7.16 59.77* 46.23* 39.62* -2.10 1.74 69.17* 45.36* 44.11* -15.59
Mitotic Spindle -9.28 -7.15 -16.70 -12.31 3.10 -3.26 -4.21 -4.48 -3.96 1.49 -9.33 -8.06 -17.10 -14.36 2.07
None 4.76 4.71 6.07 4.13 1.88 1.77 -0.70 2.66 5.00 1.47 4.85 0.92 6.15 4.82 1.63
Nuclear Periphery 4.29 -3.63 -0.17 -4.51 3.61 1.79 -3.82 2.17 0.69 4.02 3.45 -4.74 0.26 -3.85 4.77
Nuclear Periphery Foci -0.43 -6.21 -3.72 -3.45 5.63 1.90 -4.58 -1.71 -1.99 4.11 1.17 -6.87 -4.06 -4.42 6.31
Nucleolus 2.78 -0.38 5.35 1.68 2.66 0.71 -2.21 3.40 3.40 2.72 2.70 -2.15 4.56 1.66 3.27
Nucleus 8.46 -3.58 1.66 -6.66 3.81 0.99 -8.38 -1.65 -2.11 8.15 7.66 -8.71 -2.00 -7.65 8.45
Peroxisomes -4.48 -2.27 -14.64 -10.86 -5.88 -4.44 -3.26 -8.55 -7.78 -5.57 -6.24 -3.96 -17.80 -14.37 -6.81
Vacuole 3.80 -8.04 -14.94 -17.12 2.63 -7.10 -11.57 -6.39 -5.46 4.79 -1.37 -13.88 -18.80 -18.30 5.91
Vacuole Periphery -0.61 -0.41 6.45 6.67 2.31 -2.20 9.63 8.35 10.34 0.84 -1.88 2.03 7.71 9.27 1.40
Standard name
Human Ortholog
Description 3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
Localization
Cell Percentages mitochondrion (77%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Dss1

Dss1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dss1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available