Standard name | |
---|---|
Human Ortholog | |
Description | Integral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 9 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 9 | 10 | 10 | 6 | 12 | 8 | 9 | 16 | 5 | 10 | 20 | 22 |
Bud | 3 | 4 | 8 | 7 | 3 | 11 | – | 9 | 11 | 20 | 13 | 8 | 0 | 2 | 4 | 11 | 12 | 4 | 0 | 1 | 0 | 2 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | – | 4 | 4 | 5 | 5 | 4 | 0 | 0 | 0 | 1 | 2 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 11 | 8 | 5 | 3 | 0 | – | 2 | 2 | 2 | 4 | 1 | 6 | 6 | 5 | 17 | 24 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 6 | 13 | 6 | 9 | 4 | – | 6 | 10 | 7 | 3 | 3 | 3 | 7 | 12 | 51 | 50 | 16 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 11 | 10 | 4 | 3 | 1 | 3 | – | 1 | 4 | 2 | 2 | 4 | 14 | 9 | 22 | 10 | 4 | 4 | 1 | 1 | 0 | 0 | 2 | 2 |
Endosome | 13 | 6 | 29 | 16 | 35 | 0 | – | 9 | 0 | 1 | 0 | 0 | 6 | 25 | 19 | 33 | 16 | 11 | 45 | 40 | 43 | 43 | 37 | 41 |
Golgi | 29 | 4 | 8 | 2 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 24 | 48 | 52 | 21 | 73 | 49 | 42 | 40 | 18 | 19 | 34 | 42 |
Mitochondria | 326 | 310 | 207 | 197 | 263 | 333 | – | 503 | 338 | 503 | 368 | 389 | 47 | 75 | 62 | 23 | 24 | 20 | 259 | 193 | 187 | 168 | 171 | 179 |
Nucleus | 2 | 0 | 1 | 1 | 2 | 3 | – | 4 | 4 | 3 | 3 | 3 | 2 | 1 | 2 | 7 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 10 | 7 | 4 | 3 | 15 | 30 | – | 27 | 33 | 39 | 31 | 26 | 3 | 6 | 1 | 5 | 2 | 3 | 3 | 1 | 2 | 2 | 4 | 1 |
Nucleolus | 8 | 0 | 0 | 4 | 8 | 25 | – | 35 | 18 | 22 | 16 | 22 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 21 | 11 | 39 | 19 | 12 | 4 | – | 5 | 1 | 1 | 0 | 1 | 5 | 4 | 14 | 57 | 23 | 10 | 46 | 37 | 55 | 57 | 91 | 80 |
SpindlePole | 13 | 4 | 18 | 5 | 6 | 6 | – | 9 | 5 | 2 | 5 | 4 | 8 | 9 | 9 | 41 | 25 | 7 | 2 | 3 | 2 | 2 | 2 | 1 |
Vac/Vac Membrane | 40 | 23 | 10 | 7 | 29 | 23 | – | 28 | 12 | 15 | 14 | 16 | 6 | 21 | 25 | 19 | 13 | 9 | 4 | 7 | 4 | 5 | 7 | 8 |
Unique Cell Count | 382 | 338 | 273 | 223 | 312 | 355 | 541 | 358 | 527 | 375 | 406 | 97 | 174 | 182 | 252 | 242 | 123 | 421 | 350 | 326 | 324 | 381 | 393 | |
Labelled Cell Count | 494 | 405 | 350 | 276 | 390 | 445 | 642 | 442 | 622 | 465 | 481 | 133 | 223 | 237 | 304 | 288 | 153 | 421 | 350 | 326 | 324 | 381 | 393 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.8 | 5.1 | 5.1 | 5.2 | 4.5 | – | 4.9 | 4.5 | 4.4 | 4.4 | 4.3 | 6.9 | 7.2 | 7.0 | 5.5 | 6.7 | 7.2 | 6.9 | 7.1 | 7.3 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.2 | 1.1 | 1.4 | 1.2 | – | 1.2 | 1.1 | 1.4 | 1.3 | 1.1 | 0.9 | 1.2 | 0.9 | 1.0 | 1.4 | 1.4 | 1.5 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.04 | -0.17 | – | -0.07 | -0.17 | -0.22 | -0.21 | -0.26 | 0.43 | 0.5 | 0.47 | 0.12 | 0.4 | 0.49 | 0.44 | 0.48 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 |
Bud | 0.1 | -1.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.2 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -1.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | -0.1 | 2.1 | 3.3 | 2.3 |
Cytoplasm | -1.2 | -1.2 | -2.8 | – | -3.3 | -1.3 | -2.9 | -3.2 | -3.4 | 0 | -0.4 | 0.8 | 5.4 | 5.5 | 2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 4.8 | 1.8 | 0 | 0 |
Endosome | -1.3 | 0.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.3 | 1.2 | -0.1 | 0.9 | -1.6 | -0.5 |
Golgi | 0 | -1.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 6.6 | 7.7 | 7.9 | 2.7 | 8.5 | 9.7 |
Mitochondria | 3.6 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | -5.0 | -7.0 | -8.9 | -15.4 | -15.0 | -11.1 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.0 | -4.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.4 | -4.2 | -2.1 | 2.5 | -1.7 | -1.7 |
SpindlePole | -2.3 | -2.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.5 | -0.6 | -0.7 | 3.5 | 1.5 | -0.3 |
Vacuole | -0.3 | 2.7 | 1.6 | – | 1.0 | -0.2 | -0.6 | 0 | 0.2 | 0 | 3.4 | 4.0 | 1.9 | 0.9 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8693 | 4.6929 | 3.958 | 3.7502 | 3.8854 | 4.0 | 5.8054 | 6.5614 | 6.3096 | 6.1746 | 6.1369 | 6.3888 | 6.7749 | 6.9929 | 6.9145 | 7.1188 | 6.3512 | 6.8511 |
Actin | 0.0752 | 0.0161 | 0.0356 | 0.0507 | 0.0187 | 0.0117 | 0.0161 | 0.0113 | 0.0072 | 0.0072 | 0.0028 | 0.0072 | 0.0078 | 0.0163 | 0.0041 | 0.0038 | 0.0007 | 0.0015 |
Bud | 0.0012 | 0.0014 | 0.0008 | 0.008 | 0.0009 | 0.0004 | 0.0002 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0147 | 0.0005 | 0.0052 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0016 | 0.0014 | 0.0014 | 0.0254 | 0.001 | 0.0014 | 0.0003 | 0.0004 | 0.0009 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0012 | 0.0005 | 0.0016 | 0.0001 | 0.0001 |
Bud Site | 0.0206 | 0.0156 | 0.0084 | 0.0026 | 0.0012 | 0.0004 | 0.0005 | 0.0026 | 0.0018 | 0.0003 | 0.0003 | 0.0006 | 0.0008 | 0.0025 | 0.0011 | 0.0006 | 0.0001 | 0.0001 |
Cell Periphery | 0.0008 | 0.0004 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.0141 | 0.0027 | 0.0005 | 0.0012 | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0441 | 0.0224 | 0.0146 | 0.0323 | 0.0255 | 0.0049 | 0.012 | 0.014 | 0.0143 | 0.0062 | 0.0073 | 0.0063 | 0.0099 | 0.0138 | 0.0117 | 0.0043 | 0.0071 | 0.0048 |
Eisosomes | 0.0017 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0006 | 0.0019 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0055 | 0.0003 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endosome | 0.06 | 0.0477 | 0.0332 | 0.067 | 0.0534 | 0.0276 | 0.0346 | 0.0264 | 0.0195 | 0.0326 | 0.0196 | 0.021 | 0.0243 | 0.0436 | 0.0179 | 0.0108 | 0.0452 | 0.0209 |
Golgi | 0.0643 | 0.0812 | 0.0567 | 0.0593 | 0.1254 | 0.0535 | 0.0491 | 0.0664 | 0.0341 | 0.033 | 0.0422 | 0.061 | 0.0529 | 0.0899 | 0.0627 | 0.0282 | 0.1239 | 0.0557 |
Lipid Particles | 0.0587 | 0.0251 | 0.0218 | 0.0064 | 0.012 | 0.0149 | 0.0384 | 0.017 | 0.0151 | 0.0123 | 0.0184 | 0.0198 | 0.025 | 0.0105 | 0.0115 | 0.0025 | 0.0101 | 0.0157 |
Mitochondria | 0.5089 | 0.7231 | 0.7733 | 0.6808 | 0.7196 | 0.8626 | 0.7731 | 0.8055 | 0.8591 | 0.8774 | 0.8403 | 0.8138 | 0.8183 | 0.7632 | 0.8425 | 0.9185 | 0.7871 | 0.8645 |
None | 0.0128 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0025 | 0 | 0 |
Nuclear Periphery | 0.0184 | 0.0004 | 0.0005 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0013 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 |
Nucleolus | 0.003 | 0.0009 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0085 | 0.0011 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Peroxisomes | 0.0632 | 0.0446 | 0.0366 | 0.0543 | 0.0371 | 0.0126 | 0.0673 | 0.0507 | 0.0429 | 0.0133 | 0.0659 | 0.0587 | 0.0537 | 0.0519 | 0.0434 | 0.0219 | 0.0224 | 0.0324 |
Punctate Nuclear | 0.0095 | 0.0012 | 0.0003 | 0.003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0041 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Vacuole | 0.0071 | 0.0083 | 0.003 | 0.0031 | 0.0015 | 0.0021 | 0.0023 | 0.0022 | 0.0012 | 0.0067 | 0.0006 | 0.0008 | 0.0011 | 0.0022 | 0.001 | 0.0005 | 0.0008 | 0.0011 |
Vacuole Periphery | 0.0063 | 0.0053 | 0.0051 | 0.003 | 0.0018 | 0.006 | 0.0042 | 0.0017 | 0.0017 | 0.0089 | 0.0009 | 0.0018 | 0.0026 | 0.0022 | 0.0011 | 0.0015 | 0.0018 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.7753 | 20.0907 | 17.7751 | 25.5108 | 21.2939 | 25.0537 | 26.2711 | 35.7261 | 31.8611 | 31.8703 |
Translational Efficiency | 0.8411 | 0.5217 | 0.6555 | 0.5876 | 0.514 | 0.5372 | 0.5487 | 0.4586 | 0.5604 | 0.4565 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
158 | 1533 | 1741 | 651 | 2053 | 2236 | 2220 | 1520 | 2211 | 3769 | 3961 | 2171 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 993.97 | 829.77 | 836.35 | 852.54 | 770.23 | 817.85 | 833.76 | 889.81 | 786.22 | 822.70 | 834.90 | 878.63 |
Standard Deviation | 153.13 | 118.38 | 106.72 | 124.41 | 88.09 | 109.81 | 97.26 | 112.93 | 110.46 | 113.53 | 101.53 | 117.74 |
Intensity Change Log 2 | — | -0.260491 | -0.249095 | -0.221435 | — | 0.086547 | 0.114343 | 0.208208 | — | -0.098631 | -0.079071 | -0.017980 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.050992 | 0.008163 | 0.004921 | 0.064754 | 0.003556 | 0.002431 | 0.005930 | 0.046089 | 0.006946 | 0.004762 | 0.005486 | 0.051686 |
Bud Neck | 0.006198 | 0.002274 | 0.007385 | 0.009408 | 0.000609 | 0.001245 | 0.006063 | 0.006226 | 0.001008 | 0.001664 | 0.006644 | 0.007180 |
Bud Site | 0.081931 | 0.006977 | 0.019260 | 0.132702* | 0.002489 | 0.002551 | 0.031401 | 0.097442 | 0.008166 | 0.004352 | 0.026065 | 0.108015* |
Cell Periphery | 0.000941 | 0.000841 | 0.000241 | 0.000990 | 0.000238 | 0.000183 | 0.000430 | 0.000853 | 0.000288 | 0.000450 | 0.000347 | 0.000894 |
Cytoplasm | 0.023211 | 0.002028 | 0.005097 | 0.026274 | 0.001014 | 0.001173 | 0.007149 | 0.028963 | 0.002600 | 0.001521 | 0.006247 | 0.028157 |
Cytoplasmic Foci | 0.192506* | 0.061947 | 0.096065 | 0.122047 | 0.039960 | 0.037464 | 0.029973 | 0.078994 | 0.050861 | 0.047422 | 0.059023 | 0.091904 |
Eisosomes | 0.000299 | 0.000153 | 0.000100 | 0.000321 | 0.000106 | 0.000081 | 0.000087 | 0.000318 | 0.000120 | 0.000110 | 0.000093 | 0.000319 |
Endoplasmic Reticulum | 0.004820 | 0.000322 | 0.001549 | 0.004609 | 0.000238 | 0.000129 | 0.000572 | 0.000760 | 0.000565 | 0.000207 | 0.001001 | 0.001914 |
Endosome | 0.175266 | 0.106729 | 0.167299 | 0.085368 | 0.093231 | 0.099197 | 0.092400 | 0.064054 | 0.099093 | 0.102260 | 0.125321 | 0.070445 |
Golgi | 0.174201* | 0.154363* | 0.153291* | 0.130703* | 0.126326* | 0.126277* | 0.073762 | 0.145687* | 0.129747* | 0.137700* | 0.108718 | 0.141194* |
Lipid Particles | 0.004829 | 0.003686 | 0.005573 | 0.002133 | 0.003565 | 0.002173 | 0.000275 | 0.000659 | 0.003656 | 0.002788 | 0.002604 | 0.001101 |
Mitochondria | 0.081999 | 0.571537* | 0.333697* | 0.178033* | 0.681152* | 0.678290* | 0.631956* | 0.359604* | 0.638336* | 0.634869* | 0.500861* | 0.305158* |
Mitotic Spindle | 0.021894 | 0.007292 | 0.063614 | 0.062847 | 0.002412 | 0.002940 | 0.040353 | 0.027855 | 0.003804 | 0.004710 | 0.050577 | 0.038348 |
None | 0.000051 | 0.000186 | 0.000387 | 0.000199 | 0.000104 | 0.000097 | 0.000394 | 0.000170 | 0.000100 | 0.000133 | 0.000391 | 0.000179 |
Nuclear Periphery | 0.000165 | 0.000054 | 0.001034 | 0.000280 | 0.000040 | 0.000026 | 0.000078 | 0.000029 | 0.000049 | 0.000037 | 0.000498 | 0.000105 |
Nuclear Periphery Foci | 0.001209 | 0.000212 | 0.001616 | 0.000594 | 0.000108 | 0.000058 | 0.000115 | 0.000083 | 0.000186 | 0.000121 | 0.000775 | 0.000236 |
Nucleolus | 0.000141 | 0.000320 | 0.002628 | 0.000336 | 0.000219 | 0.000083 | 0.000203 | 0.000050 | 0.000213 | 0.000179 | 0.001269 | 0.000135 |
Nucleus | 0.004135 | 0.000300 | 0.004080 | 0.001346 | 0.000159 | 0.000112 | 0.000437 | 0.000485 | 0.000443 | 0.000189 | 0.002039 | 0.000743 |
Peroxisomes | 0.070517 | 0.057752 | 0.048335 | 0.122141* | 0.036451 | 0.034646 | 0.038273 | 0.112665* | 0.038885 | 0.044044 | 0.042695 | 0.115506* |
Vacuole | 0.092596 | 0.007393 | 0.057069 | 0.043080 | 0.002815 | 0.005191 | 0.025691 | 0.021616 | 0.009231 | 0.006087 | 0.039483 | 0.028052 |
Vacuole Periphery | 0.012100 | 0.007472 | 0.026756 | 0.011836 | 0.005210 | 0.005654 | 0.014458 | 0.007397 | 0.005702 | 0.006393 | 0.019863 | 0.008728 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.72 | 4.01 | -1.14 | -9.76 | -10.44 | 1.58 | -2.44 | -12.94 | -13.39 | -12.06 | 1.88 | 1.21 | -14.88 | -16.25 | -15.98 |
Bud Neck | 3.52 | -0.80 | -1.91 | -5.71 | -1.26 | -1.62 | -9.37 | -10.91 | -8.01 | -0.26 | -2.15 | -10.49 | -11.90 | -9.73 | -0.73 |
Bud Site | 6.63 | 5.51 | -3.53 | -16.39 | -14.50 | -0.12 | -15.55 | -21.73 | -21.76 | -14.02 | 3.67 | -11.17 | -25.20 | -27.00 | -20.35 |
Cell Periphery | 0.28 | 2.56 | -0.11 | -0.50 | -5.43 | 2.27 | -4.70 | -6.88 | -7.65 | -4.50 | -1.60 | -1.67 | -7.71 | -3.60 | -7.14 |
Cytoplasm | 4.14 | 3.49 | -0.46 | -11.29 | -9.43 | -0.63 | -11.56 | -18.37 | -18.24 | -13.75 | 2.58 | -6.31 | -19.48 | -21.39 | -16.85 |
Cytoplasmic Foci | 10.16 | 7.39 | 5.05 | -10.29 | -4.26 | 1.25 | 5.21 | -13.08 | -14.02 | -16.80 | 1.49 | -3.61 | -13.46 | -16.04 | -11.36 |
Eisosomes | 2.57 | 3.62 | -0.41 | -7.11 | -12.28 | 3.74 | 2.88 | -12.86 | -14.97 | -14.60 | 1.02 | 3.73 | -14.49 | -15.11 | -18.36 |
Endoplasmic Reticulum | 2.58 | 1.85 | 0.08 | -3.71 | -2.61 | 1.47 | -1.60 | -5.28 | -9.20 | -0.99 | 2.48 | -2.31 | -3.58 | -4.87 | -2.44 |
Endosome | 4.19 | 0.30 | 5.55 | 3.73 | 12.75 | -1.34 | 0.18 | 6.92 | 8.39 | 6.97 | -0.87 | -6.39 | 7.23 | 9.43 | 15.34 |
Golgi | 0.90 | 1.01 | 2.33 | 3.11 | 2.95 | 0.01 | 11.48 | -3.05 | -3.06 | -12.95 | -1.75 | 4.71 | -1.98 | -0.48 | -6.76 |
Lipid Particles | 0.78 | -0.41 | 1.75 | 2.11 | 4.92 | 2.02 | 5.95 | 4.86 | 3.08 | -2.43 | 1.40 | 1.74 | 4.53 | 4.10 | 4.51 |
Mitochondria | -26.82* | -14.29 | -5.04 | 28.43* | 12.04 | 0.27 | 4.85 | 28.75* | 28.81* | 24.14* | 0.35 | 14.48 | 32.47 | 36.70* | 21.48* |
Mitotic Spindle | 2.45 | -5.21 | -4.44 | -9.19 | -0.13 | -1.44 | -15.88 | -12.21 | -11.89 | 4.00 | -1.17 | -21.90 | -14.28 | -14.17 | 3.74 |
None | -3.14 | -3.34 | -4.14 | -0.34 | 1.77 | 0.63 | -1.63 | -2.87 | -3.90 | 1.26 | -1.38 | -2.68 | -3.76 | -2.03 | 1.95 |
Nuclear Periphery | 2.01 | -2.45 | -0.68 | -1.41 | 1.95 | 2.29 | -3.92 | 1.01 | -1.37 | 5.00 | 1.43 | -2.92 | -1.20 | -1.44 | 2.42 |
Nuclear Periphery Foci | 1.59 | -0.49 | 0.97 | -2.29 | 3.35 | 1.46 | 0.05 | 0.70 | -2.04 | 1.71 | 1.19 | -4.49 | -0.56 | -2.20 | 4.32 |
Nucleolus | -0.94 | -4.57 | -1.46 | -0.09 | 4.17 | 0.97 | 0.34 | 1.12 | 0.94 | 3.34 | 0.46 | -3.39 | 0.69 | 0.47 | 4.68 |
Nucleus | 1.33 | -0.03 | 0.94 | -4.35 | 3.43 | 0.71 | -1.58 | -2.39 | -3.73 | -0.64 | 1.23 | -3.90 | -1.38 | -5.42 | 3.49 |
Peroxisomes | 1.08 | 1.94 | -4.05 | -7.58 | -9.00 | 0.55 | -0.53 | -13.60 | -14.08 | -13.33 | -1.66 | -1.33 | -15.84 | -15.27 | -15.81 |
Vacuole | 6.14 | 2.81 | 3.51 | -8.92 | 2.42 | -4.93 | -16.31 | -10.45 | -9.02 | 1.49 | 2.89 | -16.13 | -8.87 | -12.44 | 4.54 |
Vacuole Periphery | 1.88 | -5.69 | 0.05 | -2.84 | 7.81 | -1.02 | -10.55 | -2.97 | -2.36 | 6.72 | -1.60 | -17.22 | -4.26 | -3.31 | 11.44 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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