Standard name
Human Ortholog
Description Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.92 0.97 0.99 0.98 0.96 0.93 0.89 0.85 0.85 0.79 0.73 0.99 0.99 1.0 0.98 0.95 0.96 0.73 0.8 0.65 0.84 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.1 0 0 0 0.05 0.16 0.13 0.32 0.21 0.29 0.42 0 0 0 0 0 0 0.12 0.12 0.16 0.05 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 2 0 2 1 1 1
Bud 1 1 2 0 2 1 2 3 5 3 2 3 0 0 0 0 0 1 0 0 0 0 1 2
Bud Neck 0 0 3 1 7 3 6 13 8 7 0 5 2 4 1 0 0 1 0 1 1 0 0 0
Bud Site 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 2 0 0 1 0 1 0 1 0 4 0 1 5 6 2 0 2 1 2 1
Cytoplasm 183 55 98 102 194 210 258 282 214 181 124 98 194 165 195 125 157 157 136 75 65 96 198 190
Endoplasmic Reticulum 0 2 2 0 0 1 0 0 0 2 0 0 0 0 0 3 6 11 5 0 0 1 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 1 0 0 0 0
Golgi 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Mitochondria 11 6 0 4 0 11 45 42 81 44 46 56 0 0 0 2 3 3 23 10 16 5 11 7
Nucleus 0 0 0 0 1 1 2 3 0 3 3 1 1 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 1 1 3 11 1 1 5 3 1 1 0 0 0 0 3 0 0 2 2 1
Vac/Vac Membrane 0 0 0 0 0 3 1 1 4 1 1 0 1 0 0 1 7 3 2 1 2 1 0 1
Unique Cell Count 189 60 101 103 197 218 277 318 252 213 156 134 196 166 195 128 166 164 186 94 100 114 223 214
Labelled Cell Count 196 64 106 110 207 232 317 358 313 244 181 168 199 174 196 135 185 184 186 94 100 114 223 214


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.7 4.2 4.1 4.8 3.9 3.3 4.9 3.1 3.1 3.0 3.2 4.4 4.8 4.9 5.9 6.1 6.0 4.2 4.5 4.6
Std Deviation (1e-4) 0.5 0.5 1.4 1.8 2.1 2.2 1.8 12.7 1.5 0.8 1.4 1.3 1.3 1.3 1.5 1.6 2.1 2.4 1.6 1.4 1.4
Intensity Change (Log2) – – – -0.01 0.21 -0.09 -0.32 0.22 -0.44 -0.45 -0.48 -0.4 0.09 0.2 0.22 0.49 0.54 0.52 -0.0 0.11 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0556 0.1672 -0.1188 -0.2541 -0.5854 -0.2876 -0.9041 -0.3992 -0.5748 -0.873 0.8031 -0.5482 -1.4845 -1.4982 -1.8088 -1.2709 -1.3793 -1.4134
Actin 0.0072 0.0006 0.0136 0.0018 0.005 0.0013 0.0382 0.0005 0.0169 0.0006 0.067 0.0051 0.0255 0.001 0.0027 0.0051 0.0022 0.0043
Bud 0.001 0.0016 0.0006 0.0001 0.0005 0.0003 0.0006 0.0019 0.0029 0.0005 0.0022 0.0039 0.0042 0.0002 0.0031 0.0001 0.0002 0.0015
Bud Neck 0.0024 0.0002 0.001 0.0004 0.0001 0.0007 0.0006 0.0003 0.004 0.0004 0.002 0.0007 0.0068 0 0.0002 0.0001 0.0002 0.0007
Bud Periphery 0.0021 0.0031 0.0017 0.0001 0.002 0.0006 0.001 0.0072 0.0129 0.0016 0.0056 0.007 0.0096 0.0002 0.003 0.0002 0.0002 0.0044
Bud Site 0.006 0.0032 0.0055 0.0005 0.0001 0.0004 0.004 0.0038 0.0036 0.0005 0.0046 0.0008 0.0235 0.0003 0.0043 0.0003 0.0015 0.0003
Cell Periphery 0.0005 0.0002 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0005 0 0.0004 0.0003 0.0009 0.0001 0.0001 0.0001 0 0.0002
Cytoplasm 0.0852 0.1318 0.099 0.0849 0.2097 0.0999 0.0479 0.0313 0.049 0.0982 0.0505 0.1118 0.0929 0.1594 0.16 0.0369 0.1461 0.1827
Cytoplasmic Foci 0.0347 0.0189 0.0182 0.008 0.0537 0.013 0.0073 0.0045 0.0078 0.0426 0.015 0.0156 0.0363 0.0078 0.0157 0.005 0.0337 0.0412
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0002 0.0001 0.0006 0.0002 0.0006 0.0001 0.001 0.0001 0.0007 0.0002 0.0004 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0034 0.0011 0.0007 0.0004 0.0015 0.0005 0.0021 0.0003 0.006 0.001 0.0119 0.0012 0.0027 0.0013 0.0009 0.0008 0.0016 0.0021
Endosome 0.0431 0.0067 0.0129 0.0319 0.0585 0.011 0.0191 0.0003 0.0105 0.0144 0.0453 0.0045 0.0224 0.0038 0.0046 0.0466 0.0045 0.0344
Golgi 0.0142 0.002 0.0072 0.0105 0.0271 0.0077 0.006 0.0001 0.0034 0.0045 0.0109 0.0033 0.0094 0.0017 0.0023 0.0036 0.004 0.0226
Lipid Particles 0.0186 0.0017 0.0063 0.0079 0.0154 0.0075 0.0069 0.0003 0.0029 0.0195 0.0145 0.0067 0.0248 0.0004 0.0007 0.0019 0.1025 0.0132
Mitochondria 0.0143 0.0019 0.0059 0.0185 0.0051 0.0072 0.0132 0.0042 0.016 0.002 0.0127 0.0034 0.0172 0.0019 0.003 0.1061 0.0069 0.0332
None 0.7277 0.8224 0.8078 0.8063 0.6015 0.8386 0.8133 0.9304 0.831 0.7895 0.6122 0.819 0.6762 0.8183 0.7749 0.7788 0.6267 0.6398
Nuclear Periphery 0.0031 0.0002 0.0004 0.0002 0.0009 0.0004 0.0152 0.0006 0.0114 0.001 0.0867 0.0009 0.0015 0.0004 0.0032 0.0005 0.0002 0.0013
Nucleolus 0.003 0.0002 0.0016 0.0009 0.0001 0.0005 0.002 0.001 0.0007 0.0004 0.0008 0.0009 0.0061 0.0001 0.0003 0.0002 0.0002 0.0005
Nucleus 0.0023 0.0005 0.0015 0.0005 0.002 0.001 0.0052 0.0019 0.0046 0.0009 0.0072 0.0014 0.004 0.001 0.0099 0.0004 0.0003 0.0007
Peroxisomes 0.0204 0.002 0.013 0.0094 0.0142 0.007 0.0042 0.0007 0.0075 0.0193 0.0318 0.0099 0.0194 0.0005 0.003 0.0064 0.0676 0.0078
Punctate Nuclear 0.0044 0.0009 0.0014 0.0004 0.0011 0.0009 0.008 0.0096 0.0035 0.0018 0.0106 0.0016 0.0072 0.0003 0.0012 0.0004 0.0008 0.0061
Vacuole 0.0046 0.0006 0.0009 0.0151 0.0009 0.0012 0.002 0.0004 0.0025 0.0008 0.0032 0.0013 0.0078 0.0008 0.0035 0.0047 0.0003 0.0016
Vacuole Periphery 0.0013 0.0001 0.0002 0.002 0.0003 0.0002 0.0021 0.0001 0.0018 0.0003 0.004 0.0005 0.0008 0.0001 0.0029 0.0015 0.0001 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1208.7917 1367.9257 1752.5862 1039.9303 1314.4688 1377.702 1365.7242 1458.1192 1968.2566 1129.4909
Translational Efficiency 0.9855 0.9631 0.7783 1.5838 1.0603 0.9885 1.203 1.1855 0.8545 1.2223

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1789 195 161 1549 125 1586 2179 1032 1914 1781 2340 2581

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.20 734.06 924.98 852.58 665.05 712.90 844.69 963.59 711.92 715.22 850.21 896.97
Standard Deviation 80.75 102.92 209.13 342.75 109.18 87.01 118.65 644.24 83.82 89.14 128.57 489.30
Intensity Change Log 2 — 0.037551 0.371075 0.253488 — 0.100237 0.344959 0.534957 — 0.068095 0.358551 0.395970

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000160 0.000551 0.000843 0.001230 0.000141 0.000483 0.000129 0.003657 0.000159 0.000490 0.000178 0.002200
Bud Neck 0.015877 0.023611 0.002422 0.005920 0.057727 0.041868 0.003934 0.012822 0.018610 0.039869 0.003830 0.008680
Bud Site 0.004507 0.012316 0.009756 0.032076 0.026835 0.018602 0.007740 0.066558 0.005966 0.017913 0.007879 0.045864
Cell Periphery 0.000134 0.000258 0.000410 0.000165 0.000942 0.000232 0.000121 0.000264 0.000186 0.000235 0.000141 0.000205
Cytoplasm 0.477055* 0.331164* 0.202508* 0.530414* 0.404778* 0.392682* 0.529502* 0.500500* 0.472335* 0.385946* 0.507003* 0.518453*
Cytoplasmic Foci 0.214047* 0.224083* 0.007322 0.026398 0.132707 0.256930* 0.016623 0.024663 0.208735* 0.253333* 0.015983 0.025704
Eisosomes 0.000189 0.000165 0.000082 0.000042 0.000182 0.000227 0.000034 0.000041 0.000189 0.000221 0.000037 0.000042
Endoplasmic Reticulum 0.000716 0.002292 0.009631 0.001667 0.001588 0.000548 0.001061 0.001306 0.000773 0.000739 0.001651 0.001523
Endosome 0.007371 0.018061 0.003413 0.006913 0.012714 0.020272 0.002372 0.008733 0.007720 0.020030 0.002444 0.007641
Golgi 0.002378 0.001387 0.000194 0.003868 0.000236 0.006869 0.000289 0.007616 0.002238 0.006269 0.000283 0.005366
Lipid Particles 0.006487 0.009113 0.002899 0.001242 0.004761 0.007224 0.001018 0.002536 0.006374 0.007431 0.001148 0.001760
Mitochondria 0.004117 0.007078 0.000577 0.004762 0.000527 0.013655 0.000660 0.008386 0.003882 0.012935 0.000654 0.006211
Mitotic Spindle 0.002581 0.001090 0.012074 0.063030 0.000072 0.007039 0.015537 0.051721 0.002417 0.006388 0.015299 0.058508
None 0.002002 0.002942 0.010265 0.006718 0.005214 0.001650 0.005213 0.006720 0.002211 0.001791 0.005561 0.006719
Nuclear Periphery 0.000435 0.000690 0.001900 0.001188 0.000514 0.000425 0.001341 0.000818 0.000440 0.000454 0.001380 0.001040
Nuclear Periphery Foci 0.000246 0.000860 0.002573 0.001714 0.000592 0.000688 0.000453 0.001187 0.000269 0.000707 0.000599 0.001503
Nucleolus 0.000685 0.002681 0.000981 0.000388 0.001554 0.001561 0.000401 0.000300 0.000742 0.001684 0.000441 0.000353
Nucleus 0.218449* 0.270550* 0.646363* 0.186418* 0.255861* 0.141064* 0.353669* 0.192539* 0.220892* 0.155241* 0.373808* 0.188865*
Peroxisomes 0.005424 0.022432 0.000972 0.001533 0.001456 0.022346 0.000392 0.001987 0.005165 0.022356 0.000432 0.001714
Vacuole 0.036164 0.067211 0.084461 0.121883 0.090063 0.063330 0.058852 0.103676 0.039684 0.063755 0.060614 0.114603
Vacuole Periphery 0.000976 0.001465 0.000353 0.002430 0.001536 0.002304 0.000657 0.003971 0.001013 0.002213 0.000636 0.003046

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.96 -7.39 -2.92 -0.59 2.08 -5.48 0.84 -2.40 -1.85 -2.46 -6.57 -1.63 -3.47 -2.12 -3.25
Bud Neck -1.40 9.51 9.16 3.65 -1.47 1.00 3.73 3.43 12.36 -3.68 -7.95 9.97 8.26 14.18 -3.52
Bud Site -2.15 -1.39 -7.11 -1.77 -1.22 1.07 2.79 -0.97 -3.83 -7.26 -7.05 -0.86 -9.38 -2.99 -8.74
Cell Periphery -4.32 -3.58 -0.22 4.10 3.52 2.96 3.54 3.11 0.77 -3.61 -1.49 3.51 1.17 2.31 -2.82
Cytoplasm 8.15 17.48 3.91 -6.03 -15.01 0.17 -2.47 -0.84 -2.19 3.51 9.69 4.07 4.91 -5.30 0.76
Cytoplasmic Foci -0.39 39.77 36.99 13.60 -8.10 -9.47 9.78 8.53 37.81 -5.76 -6.13 40.07 37.33 42.34 -7.56
Eisosomes 1.11 5.58 13.73 8.84 6.24 -1.75 7.06 6.45 12.38 -5.21 -1.99 15.06 14.47 13.48 -3.21
Endoplasmic Reticulum -3.57 -9.71 -8.01 1.13 8.62 3.46 0.40 0.06 -11.03 -0.97 0.08 -9.83 -8.26 -11.27 3.53
Endosome -2.31 4.18 5.69 3.13 0.93 -2.68 3.96 3.06 10.81 -2.88 -9.09 10.95 6.32 12.14 -3.85
Golgi 2.21 5.15 1.63 -0.08 -2.54 -8.15 0.63 -2.39 3.49 -2.43 -4.79 5.12 0.72 5.11 -3.45
Lipid Particles -1.70 5.33 12.37 5.00 4.70 -2.04 5.25 3.01 5.72 -3.43 -1.37 13.57 11.02 8.04 -3.57
Mitochondria -1.02 5.53 2.26 1.84 -2.09 -9.14 -1.16 -2.43 6.16 -2.30 -6.28 5.50 1.84 7.38 -3.09
Mitotic Spindle 1.61 -1.38 -9.59 -10.18 -3.64 -4.87 -7.84 -9.61 -7.78 -5.90 -2.60 -6.19 -12.92 -11.15 -7.85
None -1.04 -1.82 -5.65 -2.51 0.29 1.27 0.09 -0.41 -7.99 -2.20 1.20 -6.92 -7.44 -8.63 -1.70
Nuclear Periphery -3.20 -11.84 -19.90 -10.49 3.03 1.58 -12.36 -6.17 -7.46 1.49 -0.14 -23.88 -17.15 -13.06 2.06
Nuclear Periphery Foci -3.29 -6.44 -6.88 -2.66 2.88 -0.00 -0.10 -6.10 -3.92 -6.83 -1.33 -5.36 -10.67 -3.72 -5.93
Nucleolus -2.50 -1.07 4.22 2.81 2.74 -0.51 3.61 2.88 3.20 -2.31 -3.40 5.18 3.75 4.12 -1.52
Nucleus -2.84 -15.97 0.53 3.04 16.11 4.94 -4.98 2.14 -7.83 18.91 9.32 -23.26 1.29 -8.76 26.01
Peroxisomes -2.73 8.35 8.72 3.53 -0.36 -13.02 6.66 0.92 13.14 -4.52 -10.48 10.45 8.68 13.62 -4.29
Vacuole -4.75 -7.36 -29.89 -14.88 -4.56 2.52 -0.46 -9.31 -19.17 -14.37 -9.09 -16.96 -36.13 -29.66 -20.85
Vacuole Periphery -0.68 1.88 0.09 0.72 -1.49 -1.37 2.33 0.27 1.48 -1.63 -2.37 1.58 -0.16 2.25 -1.80
Standard name
Human Ortholog
Description Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
Localization
Cell Percentages cytoplasm (41%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Adh2

Adh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available