Standard name
Human Ortholog
Description Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.52 0.71 0.65 0.69 0.55 0.52 0.45 0.42 0.55 0.52 0.63 0.65 0.87 0.95 0.96 0.73 0.76 0.78 0.12 0.21 0.07 0.07 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.07 0 0 0 0.05 0.08 0.07 0.08 0.1 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0.06 0.06 0.08 0.13 0.12 0.08 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.45 0.3 0.46 0.44 0.58 0.55 0.57 0.58 0.54 0.51 0.42 0.43 0.11 0.05 0 0.29 0.28 0.28 0.7 0.64 0.82 0.8 0.8 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 4 2 0 2 0
Bud 1 1 0 2 0 4 9 9 8 9 13 9 0 0 0 3 1 0 1 13 10 3 4 3
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 4 0 1 0 0 0 0
Cytoplasm 82 379 320 324 301 407 450 396 469 471 422 532 87 164 158 135 158 151 20 120 34 13 13 16
Endoplasmic Reticulum 2 3 1 1 0 1 0 0 2 3 3 0 3 2 4 9 6 5 0 0 1 4 5 4
Endosome 8 9 14 13 26 11 7 8 8 6 3 6 0 0 0 0 0 2 13 31 13 4 17 15
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 1 1 3 0 2 3
Mitochondria 11 37 22 5 9 36 83 69 64 93 81 62 2 0 0 7 0 1 4 10 12 6 11 11
Nucleus 8 10 16 30 32 65 134 116 69 65 40 31 1 2 0 7 8 8 3 4 2 0 0 1
Nuclear Periphery 0 1 4 9 4 5 2 13 0 9 9 5 0 0 0 0 0 1 1 0 2 0 2 5
Nucleolus 1 1 2 0 0 2 1 1 3 3 0 2 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 0 2 0 0 0 4 1 0 0 3 0 0 0 0 0 0 1 9 2 0 3 3
Vac/Vac Membrane 71 161 224 210 321 434 564 550 458 459 283 353 11 9 4 54 58 55 119 370 406 153 290 282
Unique Cell Count 159 537 491 472 550 782 995 950 847 899 672 815 100 172 165 185 207 194 171 580 497 192 362 355
Labelled Cell Count 184 605 606 598 694 966 1251 1166 1083 1118 854 1003 105 177 167 220 233 229 171 580 497 192 362 355


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.1 4.6 4.8 6.2 5.6 5.8 6.1 5.7 5.6 5.3 5.3 4.8 5.0 4.9 5.2 5.4 5.3 5.6 5.9 6.2
Std Deviation (1e-4) 1.3 0.7 1.4 1.7 2.1 2.0 2.1 2.1 2.2 2.0 1.9 1.9 1.1 1.0 1.1 1.2 1.3 1.1 1.3 1.3 1.3
Intensity Change (Log2) – – – 0.06 0.44 0.28 0.34 0.4 0.32 0.29 0.2 0.21 0.07 0.12 0.11 0.18 0.23 0.2 0.3 0.35 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -3.9 -4.3 -4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.4935 -0.0961 -0.576 -1.1522 -0.92 -0.4668 0.2333 1.4436 1.01 0.6593 0.4258 0.7432 -1.1946 0.1787 -0.3376 -0.1862 -0.4076 0.089
Actin 0.0016 0.0009 0.0003 0.0002 0 0.0031 0.0126 0.0001 0.0068 0.0002 0.001 0.0001 0.0249 0.0001 0.0001 0.0322 0.0294 0.0013
Bud 0.0006 0.003 0.0005 0.0006 0.0003 0.0004 0.0023 0.0011 0.002 0.0013 0.0017 0.0012 0.0006 0.0004 0.0004 0.0004 0.0061 0.0004
Bud Neck 0.0011 0.0016 0.0018 0.001 0.0017 0.0062 0.001 0.0042 0.0051 0.0022 0.0022 0.0061 0.0004 0.0093 0.0007 0.0007 0.0012 0.0027
Bud Periphery 0.0013 0.0031 0.001 0.0021 0.0006 0.001 0.0033 0.0018 0.003 0.0018 0.0029 0.0016 0.0015 0.0005 0.0009 0.0005 0.0052 0.0006
Bud Site 0.0018 0.0045 0.0021 0.0016 0.0023 0.001 0.0048 0.0134 0.0146 0.0028 0.014 0.0021 0.0012 0.0063 0.0021 0.0008 0.0464 0.0015
Cell Periphery 0.0009 0.0016 0.0015 0.0007 0.0006 0.001 0.0008 0.0016 0.0016 0.0007 0.0007 0.0016 0.0006 0.0011 0.0011 0.0005 0.0018 0.0015
Cytoplasm 0.0871 0.0693 0.0512 0.0288 0.0315 0.1003 0.0998 0.1117 0.0599 0.053 0.0211 0.0908 0.1207 0.1582 0.0943 0.0591 0.0309 0.0914
Cytoplasmic Foci 0.0552 0.0309 0.0465 0.0481 0.0839 0.058 0.0785 0.0562 0.0585 0.0675 0.0442 0.063 0.0603 0.0476 0.0342 0.043 0.0288 0.0538
Eisosomes 0.0005 0.0002 0.0002 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0064 0.0059 0.0039 0.0049 0.002 0.0042 0.0082 0.0042 0.0038 0.002 0.0031 0.0035 0.008 0.0122 0.0067 0.0072 0.0015 0.0046
Endosome 0.165 0.1501 0.2293 0.2961 0.2665 0.1833 0.2452 0.1989 0.3073 0.3445 0.376 0.2641 0.2243 0.2171 0.2701 0.418 0.2475 0.2074
Golgi 0.0026 0.0043 0.0043 0.0051 0.0027 0.0027 0.0098 0.0037 0.0132 0.0048 0.0104 0.005 0.0144 0.0033 0.0042 0.0057 0.0102 0.0212
Lipid Particles 0.0133 0.012 0.0283 0.0082 0.0116 0.0138 0.0172 0.0244 0.0211 0.0194 0.0267 0.02 0.0339 0.0169 0.021 0.0192 0.0066 0.0119
Mitochondria 0.0067 0.0041 0.0133 0.0141 0.0053 0.0027 0.0169 0.005 0.0422 0.0066 0.0053 0.0077 0.0422 0.0019 0.0266 0.0025 0.0053 0.0056
None 0.4239 0.3055 0.1953 0.2272 0.1316 0.1398 0.2612 0.1197 0.1526 0.0909 0.0966 0.1267 0.2971 0.171 0.1543 0.0244 0.0035 0.0567
Nuclear Periphery 0.0078 0.0094 0.0114 0.0066 0.0037 0.0089 0.0102 0.0044 0.0052 0.0033 0.0053 0.0044 0.007 0.0083 0.0122 0.0052 0.0013 0.0064
Nucleolus 0.0029 0.006 0.0069 0.0066 0.0211 0.0066 0.0041 0.0073 0.007 0.0061 0.0086 0.0089 0.0015 0.0015 0.003 0.0104 0.0026 0.0039
Nucleus 0.007 0.0105 0.0111 0.0149 0.0241 0.0296 0.0079 0.0125 0.015 0.0075 0.0086 0.0181 0.0032 0.0042 0.0076 0.0078 0.0018 0.0262
Peroxisomes 0.0014 0.0016 0.0036 0.0027 0.0025 0.0022 0.0066 0.0012 0.0057 0.0138 0.0116 0.0025 0.0038 0.0008 0.0011 0.0011 0.0014 0.0038
Punctate Nuclear 0.0034 0.0021 0.0048 0.0016 0.0046 0.0033 0.0079 0.0017 0.003 0.0108 0.0029 0.0035 0.003 0.0014 0.0014 0.0019 0.0002 0.0007
Vacuole 0.1802 0.3419 0.3452 0.2842 0.3617 0.3953 0.1766 0.3773 0.2393 0.3317 0.3257 0.3352 0.128 0.304 0.3023 0.3194 0.5244 0.4618
Vacuole Periphery 0.0291 0.0315 0.0375 0.0446 0.0417 0.0366 0.0245 0.0494 0.0329 0.029 0.0314 0.0339 0.0225 0.0337 0.0555 0.04 0.0438 0.0367

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 706.839 309.4018 80.0748 107.6886 512.9417 401.53 226.5289 129.7351 94.2984 447.8906
Translational Efficiency 1.3777 1.7588 1.5407 1.2957 1.5029 2.1293 1.5974 1.4343 1.478 1.361

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2133 1805 550 1779 2051 1744 176 1792 4184 3549 726 3571

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.23 727.38 818.48 973.65 671.15 739.25 850.33 958.96 661.50 733.21 826.20 966.28
Standard Deviation 81.50 108.95 96.15 244.98 104.77 105.26 102.08 189.43 94.11 107.31 98.57 219.00
Intensity Change Log 2 — 0.157328 0.327566 0.578022 — 0.139427 0.341388 0.514835 — 0.148278 0.334592 0.546323

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000095 0.000393 0.003681 0.000052 0.000158 0.000680 0.005925 0.000056 0.000126 0.000463 0.004807
Bud Neck 0.031168 0.037446 0.048658 0.024218 0.033053 0.034333 0.017103 0.033900 0.032092 0.035916 0.041008 0.029077
Bud Site 0.007653 0.017197 0.027703 0.078597 0.008994 0.021746 0.011272 0.080765 0.008310 0.019432 0.023720 0.079685
Cell Periphery 0.000105 0.000138 0.000132 0.002427 0.000149 0.000656 0.000148 0.002616 0.000127 0.000393 0.000136 0.002522
Cytoplasm 0.425056* 0.241431* 0.158361* 0.338759* 0.285884* 0.072655 0.298952* 0.268215* 0.356834* 0.158494* 0.192444* 0.303359*
Cytoplasmic Foci 0.110655 0.085671 0.036964 0.046727 0.095759 0.040793 0.044361 0.055421 0.103353 0.063618 0.038757 0.051090
Eisosomes 0.000091 0.000045 0.000072 0.000085 0.000077 0.000057 0.000083 0.000110 0.000084 0.000051 0.000074 0.000097
Endoplasmic Reticulum 0.000648 0.000884 0.003736 0.010073 0.000903 0.002901 0.004151 0.008050 0.000773 0.001875 0.003837 0.009058
Endosome 0.038629 0.076619 0.125133 0.042415 0.077645 0.093672 0.045114 0.092704 0.057755 0.084999 0.105734 0.067651
Golgi 0.000970 0.002110 0.000849 0.004828 0.001955 0.004888 0.000366 0.006701 0.001453 0.003475 0.000732 0.005768
Lipid Particles 0.004933 0.003073 0.005127 0.019586 0.005437 0.004100 0.003988 0.009011 0.005180 0.003578 0.004851 0.014279
Mitochondria 0.002513 0.004409 0.001501 0.002487 0.003434 0.011407 0.000354 0.002555 0.002965 0.007848 0.001223 0.002521
Mitotic Spindle 0.001341 0.003635 0.011025 0.065214 0.001729 0.005347 0.002255 0.028786 0.001531 0.004476 0.008899 0.046934
None 0.002576 0.001383 0.002257 0.003435 0.001991 0.002701 0.004211 0.000773 0.002289 0.002031 0.002731 0.002099
Nuclear Periphery 0.000512 0.000339 0.002572 0.001460 0.000426 0.000512 0.001912 0.000437 0.000470 0.000424 0.002412 0.000947
Nuclear Periphery Foci 0.000242 0.000366 0.008685 0.004517 0.000491 0.000455 0.004287 0.002132 0.000364 0.000410 0.007619 0.003320
Nucleolus 0.001643 0.001733 0.010044 0.000929 0.001994 0.002065 0.002500 0.000751 0.001815 0.001896 0.008215 0.000840
Nucleus 0.107002 0.038771 0.278072* 0.058875 0.057834 0.030457 0.245912* 0.029486 0.082900 0.034686 0.270276* 0.044127
Peroxisomes 0.003171 0.005604 0.016735 0.007218 0.003404 0.004929 0.012725 0.011295 0.003285 0.005272 0.015763 0.009264
Vacuole 0.259278* 0.469896* 0.260101* 0.279235* 0.414721* 0.647304* 0.298956* 0.355907* 0.335476* 0.557075* 0.269520* 0.317711*
Vacuole Periphery 0.001754 0.009153 0.001880 0.005232 0.004069 0.018864 0.000671 0.004459 0.002889 0.013925 0.001587 0.004844

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.05 -3.89 -3.99 -3.92 -3.32 -2.71 -2.96 -4.38 -4.26 -3.76 -3.35 -4.90 -5.79 -5.66 -5.02
Bud Neck -2.17 -3.41 4.47 6.81 5.68 -0.60 4.04 1.44 1.93 -3.17 -1.97 -2.22 4.05 5.93 4.08
Bud Site -7.00 -4.42 -16.23 -13.00 -7.54 -7.44 -1.03 -16.74 -11.77 -14.22 -10.17 -4.44 -23.31 -17.47 -11.70
Cell Periphery -2.28 -2.46 -2.88 -2.81 -2.83 -6.80 0.34 -2.71 -1.88 -2.72 -7.05 -0.45 -3.90 -3.22 -3.89
Cytoplasm 19.29 24.35 8.19 -10.17 -16.51 29.64 0.42 -0.49 -27.62 -0.63 31.86 17.42 5.64 -24.32 -13.20
Cytoplasmic Foci 5.44 16.31 19.38 12.61 -0.27 14.86 10.34 12.64 -2.97 -2.69 13.47 19.20 22.64 7.69 -2.32
Eisosomes 7.44 1.13 2.70 -2.88 1.94 2.92 -1.65 -1.19 -2.72 0.04 7.23 -0.23 0.56 -3.81 0.70
Endoplasmic Reticulum -2.55 -11.47 -4.44 -4.26 -0.80 -10.39 -6.46 -4.34 -2.61 -0.55 -10.36 -12.99 -6.21 -4.89 -1.04
Endosome -9.98 -7.22 3.95 13.38 8.64 -3.54 7.69 1.54 4.74 -6.31 -9.01 -3.46 3.13 11.44 4.78
Golgi -3.35 1.71 -3.47 -1.12 -4.19 -3.89 4.51 -3.26 0.18 -5.78 -4.91 4.15 -4.58 -0.42 -6.83
Lipid Particles 3.44 -0.82 -4.50 -5.62 -4.14 2.06 1.86 -1.91 -2.92 -2.83 3.83 0.03 -4.83 -6.31 -4.65
Mitochondria -1.93 1.65 0.98 2.87 -0.98 -5.05 4.98 1.98 6.22 -4.51 -5.30 3.50 2.12 6.81 -2.03
Mitotic Spindle -2.53 -4.42 -14.00 -13.17 -6.91 -2.81 -1.67 -8.89 -7.19 -6.59 -3.76 -4.58 -16.48 -14.78 -6.96
None 3.12 -0.04 -1.01 -5.49 -0.99 -0.99 -2.22 3.01 2.81 4.12 0.57 -1.53 1.38 0.39 3.03
Nuclear Periphery 1.62 -10.62 -6.85 -8.59 5.54 -1.06 -10.33 -4.34 -2.52 8.83 0.62 -12.82 -7.82 -8.83 8.82
Nuclear Periphery Foci -2.02 -4.93 -8.65 -8.20 3.47 0.03 -3.94 -4.46 -4.16 2.82 -0.48 -5.59 -9.33 -8.71 4.19
Nucleolus -0.45 -5.38 2.57 4.56 5.75 -0.10 -1.08 3.25 3.83 2.45 -0.37 -5.40 4.12 5.69 5.98
Nucleus 19.25 -13.48 6.16 -10.85 15.30 9.14 -10.29 4.62 -3.87 11.05 20.37 -17.67 7.77 -10.63 19.48
Peroxisomes -3.85 -4.41 -2.46 0.84 3.52 -2.45 -1.34 -5.71 -3.59 -0.85 -4.47 -4.58 -6.05 -2.33 2.44
Vacuole -20.82 -0.19 -6.79 13.07 -4.62 -21.61 3.60 3.28 24.39 -2.16 -29.03 5.06 -2.46 25.81 -6.50
Vacuole Periphery -12.17 0.88 -2.34 7.65 -2.64 -14.59 13.03 1.37 14.20 -5.60 -18.56 7.90 -0.69 15.87 -4.82
Standard name
Human Ortholog
Description Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (65%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Scw10

Scw10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scw10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available