Standard name
Human Ortholog
Description Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.98 0.97 0.97 0.8 0.74 0.72 0.62 0.6 0.56 0.58 0.98 0.99 0.99 0.95 0.96 0.97 0.97 0.9 0.92 0.95 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.05 0.29 0.28 0.25 0.35 0.39 0.44 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.1 0.15 0.16 0.17 0.08 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 3
Bud 2 1 1 0 2 4 7 3 2 3 8 2 0 0 0 0 0 1 0 1 0 1 3 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 7 0 0 4 15 12 16 20 11 23 2 2 9 10 8 7 0 0 0 0 0 0
Cytoplasm 304 135 274 144 186 253 346 269 237 267 239 237 473 535 726 169 227 181 298 129 260 453 481 496
Endoplasmic Reticulum 3 0 1 0 0 3 4 3 11 15 3 7 5 0 0 7 4 7 1 0 1 0 3 1
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 1 0 3 1 2 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 1 1 3 2
Mitochondria 7 1 6 8 10 91 133 95 133 172 185 162 1 0 0 1 2 2 1 0 3 2 6 5
Nucleus 0 1 0 0 0 1 4 7 6 3 7 8 2 0 1 5 2 1 0 1 0 2 3 1
Nuclear Periphery 1 0 0 0 0 1 0 0 1 2 3 3 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 4 3
Vac/Vac Membrane 0 0 1 1 0 14 29 39 56 72 73 31 1 2 3 4 6 5 0 7 0 3 5 1
Unique Cell Count 312 135 279 148 191 315 469 374 382 446 424 409 483 539 730 177 237 187 308 144 283 479 527 539
Labelled Cell Count 317 138 290 153 199 371 539 429 462 555 529 475 485 541 740 196 250 205 308 144 283 479 527 539


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.9 5.5 5.7 6.6 6.5 6.6 6.9 6.7 6.8 6.4 6.6 6.6 7.5 7.6 10.0 9.5 10.0 6.9 7.7 8.1
Std Deviation (1e-4) 0.9 1.0 1.0 1.3 1.1 1.2 1.3 1.5 1.4 1.5 1.6 1.5 1.4 1.3 1.2 1.6 2.1 2.0 1.2 1.4 1.6
Intensity Change (Log2) – – – 0.04 0.27 0.25 0.26 0.32 0.29 0.31 0.22 0.27 0.27 0.44 0.46 0.86 0.79 0.86 0.33 0.48 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -1.1 0.5 0.5 1.2 1.4 0.1 2.0 0 0 0 1.7 0.6 0.8
Cytoplasm -0.6 -0.6 -6.9 -8.6 -8.9 -11.0 -11.5 -12.2 -11.9 -0.3 1.4 1.9 -1.7 -1.6 -1.0
Endoplasmic Reticulum 0 0 0 0 0 2.4 2.7 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.8 8.8 8.9 8.1 10.2 11.1 12.1 11.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 3.9 5.3 6.5 6.9 7.1 4.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8826 3.9127 3.3032 3.0434 2.8504 3.2709 2.3522 3.0284 2.5887 2.3707 2.6165 2.7454 1.7933 2.5097 1.7775 1.9095 1.6351 1.9852
Actin 0.0416 0.0016 0.0109 0.0032 0.0289 0.0041 0.0186 0.0004 0.0142 0.0107 0.0104 0.0046 0.0464 0.0073 0.0157 0.0002 0.0062 0.0056
Bud 0.0027 0.0021 0.001 0.0025 0.0005 0.0004 0.0015 0.0012 0.0007 0.0055 0.0015 0.0009 0.0005 0.0137 0.0097 0.0003 0.0005 0.0007
Bud Neck 0.0009 0.0004 0.0003 0.0046 0.0006 0.0007 0.0065 0.0002 0.0028 0.003 0.0016 0.0027 0.0041 0.0001 0.0008 0.0001 0.0007 0.0052
Bud Periphery 0.0037 0.0008 0.0007 0.0031 0.0005 0.0003 0.0017 0.0008 0.0006 0.007 0.0022 0.0011 0.0008 0.0076 0.0032 0.0002 0.0003 0.0008
Bud Site 0.0042 0.0079 0.0016 0.0014 0.0013 0.0001 0.0118 0.0093 0.0049 0.0024 0.0046 0.0003 0.0017 0.0049 0.0055 0.0001 0.0033 0.0004
Cell Periphery 0.0002 0.0003 0.0001 0.0002 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0002 0.0002 0.0002 0 0.0001 0.0001
Cytoplasm 0.6031 0.8249 0.7325 0.6501 0.717 0.7836 0.6422 0.8665 0.7674 0.811 0.7272 0.8496 0.589 0.8343 0.7793 0.7889 0.7418 0.8765
Cytoplasmic Foci 0.0356 0.0057 0.0096 0.0152 0.0218 0.0152 0.045 0.0069 0.0161 0.0132 0.0318 0.0181 0.0483 0.0076 0.0089 0.0074 0.0254 0.0145
Eisosomes 0.0007 0 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0018 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0035 0.0032 0.0039 0.003 0.0017 0.0025 0.0068 0.0015 0.0027 0.0029 0.0022 0.0015 0.0089 0.0026 0.0025 0.003 0.0028 0.0023
Endosome 0.0097 0.0057 0.0038 0.0132 0.004 0.0072 0.0373 0.0033 0.0133 0.0082 0.0221 0.0064 0.0345 0.0016 0.009 0.002 0.0333 0.0036
Golgi 0.007 0.0003 0.0006 0.0031 0.0038 0.0011 0.0096 0.0002 0.0034 0.003 0.0157 0.0011 0.0105 0.0014 0.0013 0.0002 0.0087 0.0002
Lipid Particles 0.0086 0.0002 0.0009 0.0044 0.0049 0.0006 0.0103 0.0001 0.0038 0.0017 0.0177 0.0018 0.0209 0.0117 0.0011 0 0.004 0.0002
Mitochondria 0.0043 0.0003 0.0004 0.0023 0.0016 0.0005 0.006 0.0003 0.0023 0.0009 0.0475 0.0009 0.0029 0.0007 0.0009 0.0002 0.0021 0.0002
None 0.2345 0.1356 0.2244 0.2671 0.1822 0.1737 0.1238 0.1023 0.1439 0.1157 0.0718 0.0945 0.1774 0.0972 0.1502 0.1941 0.0805 0.0805
Nuclear Periphery 0.0029 0.0015 0.0013 0.0019 0.0005 0.0017 0.012 0.0009 0.0037 0.0014 0.0023 0.0016 0.0167 0.0008 0.0009 0.0006 0.0212 0.0011
Nucleolus 0.0006 0.0002 0.0001 0.0005 0.0001 0.0001 0.0017 0.0001 0.0006 0.0002 0.0023 0.0001 0.0014 0.0001 0.0003 0 0.0003 0.0001
Nucleus 0.0025 0.0043 0.0018 0.0045 0.0013 0.0023 0.0279 0.0033 0.0074 0.0055 0.006 0.0048 0.0055 0.002 0.0049 0.0014 0.0111 0.0032
Peroxisomes 0.0249 0.0001 0.0009 0.0122 0.0269 0.0013 0.0185 0.0002 0.0045 0.0025 0.0174 0.005 0.0115 0.0048 0.0013 0.0001 0.0502 0.0004
Punctate Nuclear 0.0074 0.0031 0.0034 0.0014 0.0016 0.0039 0.0132 0.0008 0.0048 0.0027 0.012 0.0039 0.0134 0.0007 0.001 0.0005 0.0052 0.0037
Vacuole 0.001 0.0016 0.001 0.0052 0.0005 0.0007 0.0041 0.0014 0.0021 0.0019 0.0028 0.001 0.0023 0.0007 0.0028 0.0005 0.0017 0.0006
Vacuole Periphery 0.0003 0.0002 0.0002 0.0008 0.0001 0.0002 0.0012 0.0001 0.0006 0.0004 0.0009 0.0002 0.0014 0.0001 0.0004 0.0001 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6835 12.3388 9.6945 13.75 7.8952 12.9836 12.4757 11.8874 14.2201 11.5253
Translational Efficiency 1.1898 0.7774 1.0652 1.0628 0.9862 0.7904 0.8611 1.1526 0.9813 0.8789

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1780 232 396 204 1598 1749 126 123 3378 1981 522 327

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 650.21 850.84 977.66 963.63 746.84 800.58 1086.77 1129.88 695.92 806.47 1004.00 1026.16
Standard Deviation 84.52 125.67 118.67 211.88 91.69 97.67 167.70 108.31 100.34 102.63 140.18 197.25
Intensity Change Log 2 — 0.387982 0.588427 0.567574 — 0.100246 0.541176 0.597298 — 0.241323 0.563360 0.583540

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000120 0.000286 0.000207 0.000479 0.000101 0.000476 0.000253 0.000044 0.000111 0.000454 0.000218 0.000315
Bud Neck 0.013199 0.020382 0.001656 0.001711 0.005994 0.015668 0.001288 0.008161 0.009791 0.016220 0.001567 0.004137
Bud Site 0.006576 0.019968 0.003196 0.008258 0.003219 0.014719 0.003835 0.006607 0.004988 0.015334 0.003350 0.007637
Cell Periphery 0.000110 0.000097 0.000118 0.000171 0.000066 0.000129 0.000210 0.000071 0.000089 0.000125 0.000140 0.000133
Cytoplasm 0.526769* 0.524258* 0.568024* 0.582227* 0.664202* 0.521553* 0.490092* 0.783578* 0.591783* 0.521869* 0.549213* 0.657965*
Cytoplasmic Foci 0.209897* 0.183554* 0.036151 0.030457 0.121097 0.155100 0.051071 0.003652 0.167890 0.158432 0.039753 0.020375
Eisosomes 0.000104 0.000066 0.000044 0.000055 0.000041 0.000076 0.000098 0.000013 0.000074 0.000075 0.000057 0.000039
Endoplasmic Reticulum 0.001253 0.001130 0.001837 0.003492 0.000726 0.000598 0.003071 0.000016 0.001004 0.000660 0.002135 0.002184
Endosome 0.013273 0.017800 0.006183 0.005103 0.004592 0.026842 0.008309 0.000096 0.009166 0.025783 0.006696 0.003219
Golgi 0.002788 0.004549 0.000350 0.000156 0.001207 0.004296 0.000121 0.000098 0.002040 0.004326 0.000295 0.000134
Lipid Particles 0.008909 0.002685 0.001524 0.001939 0.002143 0.003848 0.002881 0.000064 0.005709 0.003712 0.001852 0.001234
Mitochondria 0.004566 0.002439 0.000145 0.000096 0.001919 0.006247 0.000112 0.000241 0.003314 0.005801 0.000137 0.000150
Mitotic Spindle 0.000367 0.002517 0.002225 0.001627 0.001748 0.020096 0.000776 0.001143 0.001020 0.018037 0.001876 0.001445
None 0.006750 0.002481 0.004141 0.003192 0.002690 0.002280 0.001655 0.025609 0.004829 0.002303 0.003541 0.011624
Nuclear Periphery 0.000448 0.000372 0.001027 0.000713 0.000307 0.000440 0.001020 0.000051 0.000381 0.000432 0.001025 0.000464
Nuclear Periphery Foci 0.000578 0.001049 0.004137 0.008140 0.000437 0.000451 0.006492 0.000040 0.000511 0.000521 0.004706 0.005093
Nucleolus 0.002475 0.001005 0.000394 0.000189 0.000828 0.001109 0.000370 0.000292 0.001696 0.001097 0.000389 0.000228
Nucleus 0.119788 0.077725 0.223340* 0.143623* 0.135634* 0.079317 0.165551* 0.140402* 0.127284 0.079130 0.209391* 0.142411*
Peroxisomes 0.003471 0.007643 0.000351 0.000330 0.001040 0.007820 0.000363 0.000227 0.002321 0.007799 0.000354 0.000291
Vacuole 0.076561 0.127038 0.144785 0.207854* 0.051487 0.136860 0.262236* 0.029550 0.064699 0.135710 0.173135 0.140785
Vacuole Periphery 0.001997 0.002958 0.000162 0.000188 0.000521 0.002076 0.000196 0.000047 0.001299 0.002179 0.000170 0.000135

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.74 -4.95 -5.19 -3.21 -3.27 -3.66 -5.63 2.28 4.36 8.15 -3.84 -6.70 -4.61 0.53 -1.41
Bud Neck -1.23 12.19 9.52 3.59 -1.60 -6.75 5.19 -0.57 2.58 -2.05 -4.65 12.53 3.90 6.84 -2.41
Bud Site -3.12 3.81 -2.23 2.00 -3.99 -7.46 -2.96 -1.27 3.08 -0.38 -7.10 1.50 -2.78 3.32 -3.36
Cell Periphery 1.32 -1.64 -3.74 -4.20 -2.70 -2.73 -7.11 -0.00 2.38 5.93 -1.73 -5.84 -3.70 -0.95 0.03
Cytoplasm 0.16 3.29 3.43 2.45 0.93 16.01 10.99 -8.02 -16.87 -14.12 9.11 10.82 0.58 -3.69 -6.37
Cytoplasmic Foci 2.74 33.45 33.64 11.54 0.19 -5.84 15.04 32.28 37.16 8.06 2.53 35.51 41.50 32.23 4.26
Eisosomes 5.20 8.86 6.27 0.43 -0.82 -8.31 -6.68 12.40 15.90 10.15 -0.03 1.89 7.10 6.24 4.56
Endoplasmic Reticulum 0.52 -6.49 -4.89 -5.05 -1.90 1.15 -7.24 8.31 9.24 9.28 3.10 -10.14 -4.11 -5.09 1.34
Endosome -1.53 7.18 8.95 5.59 0.76 -13.50 -2.88 11.33 16.94 9.06 -10.83 4.38 10.61 15.44 4.13
Golgi -0.43 7.93 8.49 1.39 0.53 -3.96 3.73 3.88 5.94 2.14 -2.86 8.16 8.96 5.82 0.82
Lipid Particles 6.29 8.26 6.51 0.07 -1.80 -2.91 -1.96 12.34 6.98 8.42 3.14 7.62 7.43 3.54 1.16
Mitochondria 1.95 6.36 6.45 2.08 3.41 -4.19 3.35 3.15 7.03 -3.58 -2.64 7.09 7.10 7.34 0.41
Mitotic Spindle -2.46 -1.95 -3.05 -1.03 -1.49 -7.11 -0.00 -0.15 6.26 -0.14 -7.63 -1.19 -2.28 6.17 -1.24
None 3.10 2.85 3.97 0.06 2.06 0.63 3.74 -4.19 -4.25 -4.45 3.23 2.74 -2.66 -3.91 -3.61
Nuclear Periphery 0.39 -12.66 -6.36 -6.41 2.55 -3.18 -9.38 7.69 11.05 12.23 -1.71 -16.40 -4.91 -3.94 7.31
Nuclear Periphery Foci -3.40 -4.61 -8.39 -7.78 -3.74 -0.29 -8.53 8.67 11.01 9.07 -0.42 -7.02 -7.63 -7.59 -0.73
Nucleolus 2.74 4.15 4.42 3.21 1.45 -0.85 1.74 1.68 3.69 -0.26 1.41 4.36 4.67 4.22 1.22
Nucleus 4.18 -12.15 -3.29 -5.41 6.15 12.98 -2.29 -0.18 -4.82 1.82 13.58 -11.44 -2.71 -8.08 6.81
Peroxisomes -2.03 6.76 6.22 4.00 -0.90 -8.79 4.91 5.27 10.06 1.34 -7.21 7.77 7.22 10.54 -0.53
Vacuole -5.91 -11.23 -12.75 -8.20 -4.82 -19.30 -12.42 3.12 15.08 12.90 -18.14 -16.34 -10.15 -4.39 2.23
Vacuole Periphery -0.54 8.06 8.02 2.10 -0.14 -7.01 5.49 7.71 9.67 7.97 -3.06 9.13 9.47 8.66 2.82
Standard name
Human Ortholog
Description Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (65%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fbp26

Fbp26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fbp26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available