Standard name
Human Ortholog
Description NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 1.0 0.98 0.97 0.95 0.84 0.8 0.83 0.78 0.81 0.78 0.99 0.99 0.99 0.99 0.97 0.97 0.92 0.95 0.88 0.97 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.11 0.09 0.09 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0.05 0.09 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 2 4 6 9 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 3 2 2 2 5 0 0 4 0 0 0 0 0 1 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 4 9 15 20 19 15 18 17 30 1 3 5 2 5 4 0 0 0 0 0 0
Cytoplasm 109 111 32 319 553 464 520 514 368 471 286 416 468 547 643 178 196 185 107 112 70 545 545 487
Endoplasmic Reticulum 0 1 0 1 4 8 58 70 39 55 22 44 2 1 3 4 8 5 0 0 0 3 3 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 1 1 0 2 0 2 1 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3 0 0 0 0 0 6 1 4 2 8 6 2 0 1 0 1 0 1 0 0 0 0 0
Nucleus 0 1 0 2 0 6 6 5 5 11 4 6 0 3 0 0 0 0 0 2 3 2 2 3
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 1 0 0 1 1 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 0 0 4 9 10 24 53 22 52 23 55 1 3 7 5 8 3 0 0 1 0 0 1
Unique Cell Count 111 112 32 324 571 490 617 645 444 602 355 536 473 553 651 180 203 190 116 119 80 560 560 506
Labelled Cell Count 112 114 32 331 577 503 641 670 461 619 371 572 475 558 664 189 218 199 116 119 80 560 560 506


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.4 23.3 22.5 23.2 23.0 20.9 19.6 20.4 19.1 18.9 18.6 19.8 27.0 28.8 25.8 40.9 41.5 39.9 22.8 22.8 23.5
Std Deviation (1e-4) 5.3 4.7 4.7 4.8 4.4 4.3 4.2 4.5 4.6 4.2 4.3 4.5 6.0 6.6 9.0 13.0 13.5 13.6 4.6 4.6 4.9
Intensity Change (Log2) – – – 0.04 0.03 -0.1 -0.2 -0.14 -0.24 -0.25 -0.27 -0.19 0.26 0.36 0.2 0.86 0.88 0.83 0.02 0.02 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -1.0 -1.3 -2.4 -2.8 -2.6 -3.0 -2.8 -3.0 -0.6 -0.6 -0.6 -0.6 -1.1 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.5492 22.2082 23.6443 22.2811 24.9284 22.5181 18.1988 26.1933 22.7219 20.035 20.4923 21.5894 17.1304 21.9768 20.0783 19.8057 17.4797 19.0694
Actin 0.0009 0.0022 0.0007 0.0003 0.0002 0.0008 0.0128 0.0016 0.0097 0.0001 0.0091 0.0012 0.0038 0.0002 0.0003 0.0004 0.0003 0.0001
Bud 0.0001 0.002 0.0011 0.0006 0 0.0003 0.0013 0.0037 0.0002 0 0.0001 0.0003 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Bud Neck 0.0003 0.0004 0.0003 0.0005 0.0004 0.0009 0.0013 0.0001 0.0048 0.0002 0.0006 0.0007 0.0003 0.0001 0.0001 0.0002 0.0004 0.0004
Bud Periphery 0 0.001 0.0005 0.0002 0 0.0001 0.0011 0.0014 0.0002 0 0.0001 0.0001 0.0003 0 0 0 0 0
Bud Site 0.0002 0.0028 0.0013 0.0002 0 0.0001 0.0019 0.0035 0.0057 0 0.0004 0 0.0003 0.0001 0 0 0.0001 0
Cell Periphery 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9533 0.9366 0.9568 0.9266 0.9816 0.9543 0.8896 0.9733 0.9419 0.9307 0.8219 0.9458 0.9305 0.991 0.9887 0.9772 0.9731 0.9714
Cytoplasmic Foci 0.0021 0.0026 0.0009 0.0028 0.0004 0.0016 0.0112 0.0012 0.0056 0.0043 0.021 0.0026 0.0095 0.0005 0.0006 0.0012 0.0057 0.0011
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0019 0.0009 0.0019 0.0011 0.0022 0.0045 0.0009 0.0007 0.0019 0.0002 0.001 0.0019 0.0004 0.0003 0.0005 0.0013 0.0006
Endosome 0.0012 0.0014 0.0006 0.0024 0.0002 0.0012 0.009 0.0046 0.0082 0.0065 0.0087 0.0034 0.0049 0.0001 0.0001 0.0003 0.0018 0.0004
Golgi 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0017 0.0012 0.0016 0.0004 0.0155 0.003 0.0007 0 0 0 0.0001 0
Lipid Particles 0.0001 0.0001 0 0.0001 0 0 0.0063 0.0007 0.0053 0.0053 0.0052 0.0068 0.0026 0 0 0 0.0001 0
Mitochondria 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0093 0.0014 0.0006 0.0001 0.0419 0.0008 0.0011 0 0 0.0001 0.0001 0.0001
None 0.0015 0.0013 0.0008 0.0014 0.0002 0.001 0.0077 0.0007 0.0005 0.0003 0.0004 0.0158 0.0036 0.0004 0.0006 0.001 0.0008 0.0004
Nuclear Periphery 0.0056 0.0073 0.0039 0.0066 0.0024 0.0047 0.0053 0.0008 0.001 0.0332 0.0006 0.0025 0.0068 0.0007 0.0007 0.0019 0.0017 0.0024
Nucleolus 0.0001 0.0002 0.0001 0.0001 0 0 0.0008 0.0001 0.0001 0.0001 0 0 0.0005 0 0 0 0 0
Nucleus 0.0298 0.0333 0.0298 0.0523 0.0127 0.0297 0.0176 0.003 0.0072 0.0104 0.0095 0.0135 0.0232 0.0059 0.0082 0.0166 0.0124 0.0223
Peroxisomes 0.0001 0.0002 0.0001 0.0002 0 0 0.0103 0.0001 0.005 0.0001 0.0634 0.0015 0.0007 0 0 0 0.0001 0
Punctate Nuclear 0.0014 0.0041 0.001 0.0016 0.0002 0.0012 0.0033 0.0002 0.0004 0.005 0.0004 0.0005 0.0075 0.0001 0.0001 0.0003 0.0014 0.0003
Vacuole 0.0012 0.0019 0.0009 0.0017 0.0003 0.0012 0.0036 0.0009 0.0009 0.0006 0.0003 0.0004 0.0011 0.0002 0.0001 0.0001 0.0005 0.0003
Vacuole Periphery 0.0002 0.0003 0.0001 0.0003 0.0001 0.0002 0.0011 0.0003 0.0002 0.0008 0.0003 0.0002 0.0005 0 0 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 235.3522 199.4611 179.0973 183.7542 167.7634 188.3812 200.2936 205.4185 198.8806 162.7166
Translational Efficiency 2.0585 1.9772 1.7106 1.6485 1.5719 2.3409 1.8811 1.5614 1.5483 1.6761

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
93 533 529 1764 1049 9 1351 227 1142 542 1880 1991

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2341.50 1841.07 2378.09 2965.04 1678.56 287.71 2569.92 3233.17 1732.55 1815.28 2515.94 2995.61
Standard Deviation 581.17 504.84 517.93 879.56 301.67 7.52 555.47 674.20 379.44 538.54 551.95 862.85
Intensity Change Log 2 — -0.346889 0.022370 0.340619 — -2.544537 0.614499 0.945725 — -0.917190 0.299631 0.624635

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001878 0.000354 0.000241 0.000300 0.000192 0.000005 0.000112 0.000318 0.000329 0.000348 0.000148 0.000302
Bud Neck 0.007085 0.000776 0.001264 0.002201 0.000440 0.000003 0.000981 0.001894 0.000981 0.000763 0.001061 0.002166
Bud Site 0.019352 0.003377 0.001371 0.005633 0.001068 0.000008 0.001481 0.006220 0.002557 0.003322 0.001450 0.005700
Cell Periphery 0.006702 0.000339 0.000404 0.000307 0.000127 0.000028 0.000116 0.000783 0.000662 0.000334 0.000197 0.000361
Cytoplasm 0.662190* 0.807310* 0.588802* 0.720081* 0.839913* 0.000017 0.640703* 0.644160* 0.825440* 0.793905* 0.626099* 0.711425*
Cytoplasmic Foci 0.042072 0.011157 0.007890 0.003403 0.008841 0.000006 0.002479 0.015628 0.011547 0.010972 0.004002 0.004797
Eisosomes 0.000013 0.000002 0.000001 0.000002 0.000001 0.000011 0.000001 0.000002 0.000002 0.000002 0.000001 0.000002
Endoplasmic Reticulum 0.002269 0.000369 0.000077 0.000140 0.000368 0.000000 0.000083 0.000034 0.000522 0.000363 0.000082 0.000128
Endosome 0.001337 0.000724 0.000103 0.000941 0.000414 0.000001 0.000136 0.000078 0.000489 0.000712 0.000127 0.000843
Golgi 0.011032 0.001501 0.000419 0.001786 0.000518 0.000003 0.000192 0.000334 0.001374 0.001476 0.000256 0.001621
Lipid Particles 0.008861 0.000539 0.000621 0.000599 0.000516 0.000010 0.000242 0.001384 0.001196 0.000530 0.000349 0.000689
Mitochondria 0.000744 0.000119 0.000182 0.000308 0.000068 0.000007 0.000214 0.000116 0.000123 0.000118 0.000205 0.000286
Mitotic Spindle 0.003487 0.001235 0.002877 0.016079 0.000953 0.000164 0.001314 0.000465 0.001160 0.001217 0.001754 0.014299
None 0.006049 0.009981 0.010011 0.012199 0.010353 0.999665* 0.013275 0.010558 0.010003 0.026415 0.012357 0.012012
Nuclear Periphery 0.000320 0.000097 0.000246 0.000309 0.000115 0.000000 0.000207 0.000231 0.000131 0.000095 0.000218 0.000300
Nuclear Periphery Foci 0.007930 0.004936 0.001541 0.002122 0.005729 0.000024 0.000752 0.002090 0.005908 0.004855 0.000974 0.002118
Nucleolus 0.000706 0.000150 0.000117 0.000558 0.000061 0.000021 0.000146 0.000081 0.000114 0.000148 0.000138 0.000503
Nucleus 0.189336* 0.144997* 0.375943* 0.221949* 0.124466 0.000007 0.328287* 0.299349* 0.129749 0.142590* 0.341697* 0.230773*
Peroxisomes 0.000154 0.000036 0.000026 0.000067 0.000020 0.000019 0.000011 0.000019 0.000031 0.000036 0.000015 0.000061
Vacuole 0.024745 0.010825 0.007781 0.010246 0.005746 0.000000 0.008884 0.016155 0.007294 0.010646 0.008574 0.010920
Vacuole Periphery 0.003739 0.001173 0.000079 0.000769 0.000091 0.000000 0.000383 0.000103 0.000388 0.001154 0.000298 0.000693

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.65 3.75 4.18 2.90 2.83 NaN NaN NaN NaN NaN -0.91 1.96 2.36 2.82 0.97
Bud Neck 4.63 3.60 4.02 -4.21 1.70 NaN NaN NaN NaN NaN 0.57 -5.79 -4.17 -4.98 1.38
Bud Site 3.91 4.70 4.23 0.25 -2.26 NaN NaN NaN NaN NaN -1.23 0.70 -2.42 0.11 -3.14
Cell Periphery 2.86 2.70 2.88 0.85 5.95 NaN NaN NaN NaN NaN 1.58 1.56 1.56 -0.23 0.01
Cytoplasm -5.79 2.38 -1.82 12.88 -11.75 NaN NaN NaN NaN NaN 3.94 36.30 21.96 11.25 -15.18
Cytoplasmic Foci 3.89 3.56 4.98 4.61 6.47 NaN NaN NaN NaN NaN -0.58 3.23 3.78 3.19 0.65
Eisosomes 3.92 3.67 4.01 0.54 2.18 NaN NaN NaN NaN NaN -0.48 -1.23 -0.17 0.40 1.39
Endoplasmic Reticulum 1.65 1.89 1.93 3.59 1.75 NaN NaN NaN NaN NaN 1.37 4.29 4.74 3.64 2.58
Endosome 2.18 4.36 3.49 1.20 -2.14 NaN NaN NaN NaN NaN -0.64 4.53 1.53 1.34 -1.81
Golgi 1.84 2.01 1.98 1.20 -0.61 NaN NaN NaN NaN NaN -0.12 2.45 1.33 1.29 -1.80
Lipid Particles 3.00 2.73 2.90 -2.06 2.23 NaN NaN NaN NaN NaN 1.98 1.09 0.27 -3.08 -1.53
Mitochondria 3.82 3.13 3.06 -3.34 -0.22 NaN NaN NaN NaN NaN 0.26 -7.05 -3.55 -3.24 1.07
Mitotic Spindle 0.99 -0.16 -3.20 -5.18 -3.25 NaN NaN NaN NaN NaN -0.82 -3.17 -5.82 -5.08 -3.59
None -1.97 -1.77 -2.33 -0.36 -1.50 NaN NaN NaN NaN NaN -2.83 -0.56 0.42 2.93 1.61
Nuclear Periphery 3.70 -0.87 0.78 -6.35 2.57 NaN NaN NaN NaN NaN 1.54 -13.52 -6.64 -7.34 2.33
Nuclear Periphery Foci 1.23 2.80 3.23 5.91 2.40 NaN NaN NaN NaN NaN 0.87 7.99 7.62 5.65 -0.04
Nucleolus 1.22 1.20 0.70 -1.63 -1.65 NaN NaN NaN NaN NaN -0.61 -2.74 -1.97 -1.60 -1.12
Nucleus 2.35 -7.87 -1.84 -12.04 14.28 NaN NaN NaN NaN NaN -2.05 -38.87 -19.28 -13.01 20.60
Peroxisomes 1.53 1.52 1.56 0.23 0.15 NaN NaN NaN NaN NaN 0.51 0.90 0.64 0.35 -0.58
Vacuole 2.73 1.51 -0.41 -9.58 -4.97 NaN NaN NaN NaN NaN -2.87 -10.66 -15.37 -10.86 -5.61
Vacuole Periphery 1.26 1.93 1.79 0.79 -2.18 NaN NaN NaN NaN NaN -0.77 0.91 0.24 0.82 -0.83
Standard name
Human Ortholog
Description NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Adh6

Adh6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adh6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available