Standard name
Human Ortholog
Description Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.11 0.06 0.16 0.19 0.22 0.17 0.3 0.25 0.12 0.17 0.07 0.18 0 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.14 0 0 0 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0.18 0.07 0.08
Mitochondria 0.05 0 0.13 0.05 0.05 0.06 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.19 0.18 0.22 0.21 0.1 0.21 0.29 0.22 0.2 0.16 0.11 0 0.06 0.07 0.23 0.1 0.09 0.1 0.07 0.06 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.08 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.74 0.85 0.73 0.75 0.83 0.67 0.66 0.69 0.74 0.69 0.71 0.8 0.74 0.82 0.6 0.82 0.74 0.85 0.88 0.83 0.68 0.66 0.73
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 1 1
Bud 0 0 0 0 0 0 0 1 2 0 1 0 0 1 1 0 0 0 0 3 2 5 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 9 21 21 51 98 96 102 106 104 20 33 15 13 1 3 1 1 4 0 5 3
Endoplasmic Reticulum 1 0 0 1 2 1 0 0 2 2 2 2 0 0 3 2 0 0 0 0 0 1 1
Endosome 0 1 0 0 2 1 1 0 2 0 0 1 14 27 0 0 0 0 0 2 2 8 7
Golgi 0 2 2 1 4 0 4 0 2 1 0 13 7 2 3 1 1 0 0 1 14 12 14
Mitochondria 7 3 24 9 16 20 16 6 11 17 16 5 3 1 4 1 1 1 0 0 1 4 3
Nucleus 27 25 39 41 34 68 148 96 116 55 46 6 12 13 17 4 2 14 11 12 1 6 6
Nuclear Periphery 1 0 3 1 1 0 3 0 0 1 0 1 0 1 0 0 0 0 0 2 0 3 1
Nucleolus 5 3 3 2 10 4 11 4 4 7 1 9 7 6 2 3 0 2 1 2 1 7 5
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 4 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 103 121 133 146 288 218 341 293 434 244 293 128 145 164 44 32 17 123 133 169 55 119 144
Unique Cell Count 139 142 181 194 347 325 517 427 590 353 413 161 195 200 73 39 23 146 151 205 82 181 197
Labelled Cell Count 149 159 213 222 380 364 622 496 675 434 463 187 221 234 88 44 24 146 151 205 82 181 197


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.3 35.4 19.9 24.9 35.2 30.7 28.8 28.1 28.6 25.9 25.0 24.0 17.7 14.9 56.4 52.8 54.1 23.0 27.1 26.6
Std Deviation (1e-4) 11.8 12.5 8.2 10.1 14.0 16.5 14.6 15.5 14.7 12.9 12.8 10.8 7.8 7.1 33.8 22.3 28.3 9.9 13.6 11.8
Intensity Change (Log2) 0.32 0.82 0.63 0.53 0.5 0.52 0.38 0.33 0.27 -0.17 -0.42 1.5 1.41 1.44 0.21 0.45 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 0.5 3.6 4.5 5.2 4.1 6.7 5.8 2.5 3.7 1.0 3.3 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 3.7 5.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0
Mitochondria -2.9 -3.6 -2.7 -5.1 -6.2 -6.4 -3.5 -4.2 -3.4 -4.4 -5.0 -1.8 0 0
Nucleus -0.1 -3.7 -0.2 1.9 0.3 -0.6 -1.7 -3.3 -4.9 -4.4 -4.3 0.3 -1.6 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 2.0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 2.6 -1.5 -1.9 -1.2 0 -1.0 -0.6 1.3 0.2 2.0 -2.1 1.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.2912 24.8568 20.9217 20.3554 17.6349 23.0594 14.1553 23.5251 19.7756 18.5567 17.6232 23.7671 15.3928 27.2499 24.3457 21.2749 21.1072 28.7351
Actin 0.0196 0 0.0004 0.0065 0.0001 0 0.0116 0 0.003 0.0017 0.0182 0 0.0084 0.0001 0.0009 0 0.0002 0.0001
Bud 0.0007 0.0001 0.0002 0.0003 0.0003 0.0001 0.0004 0.0007 0.0002 0.0003 0.0015 0.0001 0.0004 0.0002 0.0002 0.0004 0.0004 0.0001
Bud Neck 0.0036 0.0001 0.0003 0.0001 0.0002 0.0001 0.001 0.0003 0.0003 0.0002 0.0005 0.0002 0.0003 0.0004 0.0004 0.0007 0.0003 0.0003
Bud Periphery 0.0013 0.0003 0.0007 0.0005 0.0005 0.0003 0.0008 0.0006 0.0005 0.0007 0.0021 0.0004 0.0006 0.0005 0.0008 0.0011 0.001 0.0005
Bud Site 0.0036 0.0002 0.0003 0.0013 0.0003 0 0.0011 0.0004 0.0002 0.0007 0.0022 0.0001 0.0016 0.0009 0.0004 0.0006 0.0005 0.0001
Cell Periphery 0.0093 0.0087 0.0083 0.0075 0.003 0.0126 0.0113 0.013 0.0126 0.0068 0.0076 0.0143 0.0138 0.0169 0.0114 0.0179 0.0128 0.0158
Cytoplasm 0.0101 0.0024 0.0021 0.0006 0.0005 0.0001 0.0036 0.0005 0.0002 0.001 0.0022 0.0002 0.011 0.002 0.0006 0.0003 0.004 0.0002
Cytoplasmic Foci 0.0228 0.0012 0.0031 0.0103 0.003 0.0001 0.0157 0.0002 0.001 0.0142 0.0129 0.0001 0.0157 0.003 0.0067 0.0008 0.0061 0.0035
Eisosomes 0.0001 0 0 0.0003 0 0 0.0001 0 0.0001 0.0004 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0051 0.0003 0.0009 0.0011 0.0002 0.0002 0.0008 0.0003 0.0007 0.0014 0.0017 0.0004 0.0035 0.0003 0.0008 0.0002 0.0023 0.0003
Endosome 0.0601 0.0098 0.0241 0.0068 0.0527 0.0035 0.0216 0.0058 0.0144 0.0422 0.0587 0.0035 0.0543 0.0066 0.0242 0.0116 0.0486 0.0082
Golgi 0.0127 0.0028 0.0036 0.0032 0.0241 0.0002 0.0137 0.0004 0.0035 0.0041 0.0225 0.0001 0.0087 0.0005 0.0067 0.0004 0.0072 0.0065
Lipid Particles 0.0385 0.0105 0.0174 0.0278 0.0039 0.0075 0.0223 0.0037 0.0163 0.0183 0.0178 0.0022 0.0371 0.0037 0.0129 0.0094 0.0189 0.0224
Mitochondria 0.0201 0.0049 0.0218 0.0569 0.0118 0.003 0.0247 0.0024 0.0383 0.0318 0.067 0.0012 0.0471 0.0023 0.0329 0.0024 0.0101 0.032
None 0.0035 0.0014 0.0001 0.0012 0 0 0.0008 0 0.0002 0.0014 0.0001 0 0.0044 0.0002 0.0002 0 0.0024 0
Nuclear Periphery 0.0132 0.0012 0.0122 0.0011 0.0006 0.0026 0.0088 0.0022 0.0013 0.0013 0.0013 0.0021 0.0186 0.006 0.0017 0.0006 0.0088 0.0007
Nucleolus 0.014 0.0042 0.0172 0.0018 0.0013 0.0023 0.0206 0.0034 0.006 0.0064 0.0011 0.0016 0.0147 0.0137 0.0048 0.0177 0.0078 0.0038
Nucleus 0.0049 0.002 0.0038 0.0011 0.0013 0.0014 0.01 0.0023 0.0018 0.002 0.0007 0.0016 0.0052 0.0037 0.0019 0.0033 0.0046 0.0018
Peroxisomes 0.0036 0.0001 0.0008 0.0053 0.0011 0 0.0087 0.0001 0.0004 0.0031 0.0052 0 0.0033 0.0003 0.0008 0.0003 0.0007 0.0008
Punctate Nuclear 0.0029 0.0001 0.0001 0.0007 0 0 0.0012 0 0 0.0006 0.0004 0 0.0048 0.0001 0.0002 0 0.0006 0
Vacuole 0.3559 0.4824 0.4122 0.489 0.4876 0.471 0.4143 0.5072 0.4447 0.4563 0.3959 0.5427 0.3741 0.5004 0.449 0.5439 0.5291 0.5381
Vacuole Periphery 0.3943 0.4671 0.4702 0.3767 0.4073 0.495 0.407 0.4565 0.4543 0.4051 0.3805 0.4291 0.3724 0.438 0.4424 0.3882 0.3333 0.3648

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 149.5909 139.8593 149.2999 154.4917 167.2606 150.8797 153.1959 192.561 184.4237 166.7941
Translational Efficiency 1.4596 1.4255 1.588 1.7621 1.4152 2.0226 1.6877 1.5178 1.8047 1.4557

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1310 1266 975 1395 904 1990 2177 1799 2214 3256 3152 3194

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1654.02 1994.21 1719.50 2798.47 2736.62 1843.29 2004.59 2566.85 2096.06 1901.97 1916.40 2668.01
Standard Deviation 541.90 555.80 285.54 683.40 1031.75 457.79 346.02 515.72 944.22 503.60 353.95 605.79
Intensity Change Log 2 0.269841 0.056012 0.758662 -0.570112 -0.449088 -0.092396 -0.194264 -0.237543 0.289233

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000183 0.000077 0.000304 0.004606 0.000151 0.000091 0.000690 0.004203 0.000170 0.000086 0.000570 0.004379
Bud Neck 0.002057 0.003314 0.004522 0.013732 0.000960 0.002458 0.005839 0.014847 0.001609 0.002791 0.005432 0.014360
Bud Site 0.008201 0.004247 0.005410 0.013303 0.000711 0.002114 0.009840 0.019262 0.005143 0.002943 0.008469 0.016659
Cell Periphery 0.030280 0.016144 0.003348 0.250824* 0.034753 0.012142 0.028699 0.167145* 0.032106 0.013698 0.020857 0.203693*
Cytoplasm 0.002542 0.000335 0.000538 0.000649 0.000795 0.000488 0.000183 0.001984 0.001829 0.000429 0.000293 0.001401
Cytoplasmic Foci 0.001454 0.002272 0.011866 0.000892 0.001912 0.000912 0.003318 0.004429 0.001641 0.001441 0.005962 0.002884
Eisosomes 0.000252 0.001458 0.000062 0.003175 0.000816 0.001571 0.000150 0.001603 0.000482 0.001527 0.000122 0.002289
Endoplasmic Reticulum 0.002597 0.001462 0.001232 0.078750 0.000649 0.001612 0.018913 0.057382 0.001802 0.001553 0.013444 0.066715
Endosome 0.006135 0.002527 0.042828 0.026486 0.000451 0.003444 0.142263 0.075911 0.003814 0.003087 0.111505 0.054325
Golgi 0.005767 0.001600 0.034685 0.017807 0.000194 0.000752 0.042010 0.035493 0.003491 0.001082 0.039744 0.027769
Lipid Particles 0.001117 0.001184 0.007936 0.202388* 0.000290 0.001494 0.035768 0.212508* 0.000779 0.001374 0.027159 0.208088*
Mitochondria 0.001301 0.001598 0.001969 0.001008 0.000446 0.003482 0.001119 0.001543 0.000952 0.002749 0.001382 0.001309
Mitotic Spindle 0.000030 0.000109 0.000420 0.000384 0.000005 0.000450 0.000495 0.000596 0.000020 0.000318 0.000472 0.000504
None 0.000931 0.000196 0.000076 0.000070 0.000390 0.000162 0.000027 0.000068 0.000710 0.000175 0.000042 0.000069
Nuclear Periphery 0.000081 0.000302 0.001807 0.002253 0.000021 0.000339 0.000345 0.000945 0.000056 0.000324 0.000797 0.001516
Nuclear Periphery Foci 0.000441 0.000662 0.006642 0.002456 0.000168 0.000661 0.002323 0.002887 0.000330 0.000662 0.003659 0.002699
Nucleolus 0.002839 0.005345 0.004681 0.000137 0.001186 0.005140 0.000267 0.000166 0.002164 0.005220 0.001632 0.000153
Nucleus 0.001837 0.001530 0.001257 0.000162 0.004267 0.001576 0.000082 0.000186 0.002829 0.001558 0.000445 0.000175
Peroxisomes 0.000166 0.000235 0.000905 0.001498 0.000042 0.000264 0.000115 0.001524 0.000115 0.000253 0.000360 0.001513
Vacuole 0.771993* 0.854058* 0.703034* 0.364125* 0.924914* 0.889980* 0.649947* 0.380211* 0.834432* 0.876013* 0.666368* 0.373185*
Vacuole Periphery 0.159796* 0.101343* 0.166480* 0.015298 0.026880 0.070869 0.057607 0.017106 0.105525* 0.082718 0.091285 0.016316

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.09 -1.60 -6.05 -6.20 -5.88 0.79 -4.63 -7.45 -7.62 -6.38 1.89 -5.19 -9.53 -9.75 -8.53
Bud Neck -2.28 -4.93 -11.24 -9.23 -8.25 -4.01 -11.91 -16.39 -13.90 -9.85 -3.65 -11.50 -19.25 -16.51 -12.56
Bud Site 3.02 2.07 -2.94 -5.21 -4.56 -3.38 -13.87 -11.37 -10.33 -5.45 3.01 -4.39 -9.01 -11.38 -6.63
Cell Periphery 5.38 11.75 -33.21* -36.46* -39.26* 8.65 2.21 -23.64 -30.23* -26.53* 10.09 5.94 -39.61 -46.42* -44.28*
Cytoplasm 2.36 2.18 1.76 -1.50 -0.98 0.93 2.08 -1.96 -2.80 -3.65 2.48 2.88 0.48 -3.14 -3.90
Cytoplasmic Foci -0.86 -7.03 1.76 1.44 7.50 0.79 -0.89 -1.61 -4.84 -1.17 0.31 -5.01 -1.76 -2.55 4.12
Eisosomes -8.38 8.27 -10.21 -5.36 -10.90 -4.69 6.97 -2.61 -0.07 -5.11 -9.92 8.58 -8.70 -3.35 -10.65
Endoplasmic Reticulum 5.83 6.22 -19.87 -20.17 -20.22 -7.77 -15.86 -20.66 -20.31 -12.91 1.98 -14.31 -28.38 -28.51 -21.95
Endosome 3.76 -12.15 -10.77 -13.58 4.86 -4.94 -34.41* -24.23 -22.96 12.84 1.11 -34.40* -25.10 -25.78 15.63
Golgi 3.61 -9.20 -6.64 -10.43 5.09 -2.33 -19.18 -16.66 -16.31 2.16 3.62 -19.38 -16.17 -19.27 5.38
Lipid Particles -0.46 -5.12 -26.24 -26.23* -24.97* -6.75 -15.21 -31.54* -31.35* -24.73* -4.48 -15.71 -40.98 -40.85* -33.85*
Mitochondria -0.85 -1.87 0.78 1.56 2.57 -4.55 -2.52 -2.69 2.65 -1.21 -4.17 -2.04 -1.38 3.09 0.15
Mitotic Spindle -0.92 -2.11 -2.98 -1.87 0.43 -2.29 -1.46 -2.76 -0.57 -0.31 -2.41 -1.96 -3.66 -1.08 -0.09
None 2.98 3.54 3.54 4.12 0.12 1.29 2.03 1.76 3.32 -2.13 3.30 4.16 3.95 5.27 -2.29
Nuclear Periphery -3.99 -4.95 -17.44 -14.27 -1.09 -5.96 -15.99 -18.09 -8.23 -10.86 -6.86 -6.76 -23.32 -16.14 -5.51
Nuclear Periphery Foci -1.51 -5.00 -4.36 -3.72 3.16 -2.76 -9.61 -5.92 -4.93 -1.16 -2.95 -8.09 -7.28 -6.18 1.88
Nucleolus -3.14 -1.83 5.80 7.85 4.99 -5.23 3.51 3.88 7.11 7.53 -5.27 1.28 6.80 10.16 5.20
Nucleus 0.48 0.97 3.59 7.26 2.99 1.66 2.54 2.47 7.93 -3.82 1.72 3.22 3.59 10.68 2.25
Peroxisomes -0.96 -4.85 -5.13 -4.70 -1.93 -2.83 -3.11 -6.81 -5.46 -6.48 -2.44 -4.78 -8.35 -7.18 -6.63
Vacuole -9.84 7.19 41.38* 53.45* 32.57* 5.94 37.68* 64.95* 65.53* 30.10 -7.68 26.37 67.52* 85.12* 43.03*
Vacuole Periphery 8.04 -0.80 24.65* 18.01 27.34* -11.03 -9.16 3.21 18.47 20.82* 5.03 3.28 22.80 25.63 31.73*
Standard name
Human Ortholog
Description Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles
Localization
Cell Percentages vacuole (52%), cytoplasm (9%), mixed (25%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (0.00048)
Subcompartmental Group N/A

Sna3

Sna3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sna3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available