Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0.05 0.06 0.06 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0.06 0.05 0.07 0.05 0 0 0.13 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 1.0 – 1.0 0.99 0.93 0.95 0.77 0.7 0.74 0.78 0.76 0.71 0.96 0.97 0.99 0.95 0.92 0.98 0.92 0.77 0.83 0.89 0.86 0.88
Endoplasmic Reticulum 0 – 0 0 0.05 0 0.16 0.2 0.17 0.13 0.15 0.19 0 0 0 0 0.05 0 0 0 0 0 0.09 0.07
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 2 5 8 6 8 7 6 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 1 3 5 8 10 5 4 2 9 5 6 3 11 6 1 0 0 0 0 0 0
Cytoplasm 72 – 16 80 154 84 114 105 76 105 52 49 204 232 318 193 104 141 78 22 26 93 202 223
Endoplasmic Reticulum 0 – 0 0 9 2 23 30 18 17 10 13 9 4 5 8 6 5 2 0 0 3 22 17
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 3 2 1 1 1 1 1 2 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 – 0 0 0 0 1 2 6 5 5 1 1 0 0 1 1 0 0 1 0 0 2 2
Nucleus 0 – 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0 0 1 0 0 1 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 1 1 0 1 4 0 2 0 0 0 1 0 4 2 1 0 1 1 0 0 1
Unique Cell Count 72 16 81 165 88 148 150 103 135 68 69 212 238 322 203 113 144 85 29 32 105 235 255
Labelled Cell Count 72 16 82 167 93 156 161 115 143 78 80 221 245 326 217 119 149 85 29 32 105 235 255


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 77.3 – 85.9 80.8 60.1 48.3 41.8 40.2 35.3 36.8 34.4 36.0 95.4 90.9 92.4 141.1 135.8 132.5 81.4 84.4 87.7
Std Deviation (1e-4) 15.8 – 13.8 21.9 17.1 11.6 10.6 11.1 10.9 9.7 12.6 8.5 17.7 20.7 21.7 52.9 52.0 49.9 23.6 21.7 22.7
Intensity Change (Log2) – – – -0.09 -0.52 -0.83 -1.04 -1.1 -1.28 -1.22 -1.32 -1.26 0.15 0.08 0.1 0.71 0.66 0.62 -0.08 -0.03 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -1.1 -0.9 -2.2 -2.6 -2.3 -2.1 -2.2 -2.5 -0.8 -0.6 -0.4 -0.9 -1.2 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0059 0.001 0.0014 0.001 0.0411 0.0043 – – – – – – 0.0009 0.0003 0.0352 0.0009 0.0038 0.0253
Bud 0.0031 0.001 0.0044 0.0024 0.0021 0.0039 – – – – – – 0.0008 0.0006 0.0167 0.0035 0.0178 0.0054
Bud Neck 0.0006 0.0005 0.0006 0.0012 0.0119 0.0026 – – – – – – 0.0006 0.0002 0.0004 0.0009 0.0021 0.0016
Bud Periphery 0.0046 0.0007 0.0063 0.0016 0.0037 0.0036 – – – – – – 0.0003 0.0001 0.0229 0.0004 0.0036 0.0035
Bud Site 0.0025 0.003 0.0023 0.0007 0.0111 0.0012 – – – – – – 0.0002 0.0006 0.0025 0.0001 0.0036 0.0004
Cell Periphery 0.0193 0.0271 0.0364 0.0085 0.0051 0.0064 – – – – – – 0.0013 0.0011 0.0061 0.0006 0.0039 0.0012
Cytoplasm 0.6742 0.8834 0.7402 0.8417 0.6711 0.6644 – – – – – – 0.7843 0.9857 0.7373 0.9151 0.9088 0.7248
Cytoplasmic Foci 0.017 0.0037 0.0051 0.0035 0.0052 0.0058 – – – – – – 0.0017 0.0007 0.0018 0.0014 0.0009 0.007
Eisosomes 0.0004 0.0003 0.0003 0 0.005 0.0008 – – – – – – 0 0 0.0015 0 0 0.0002
Endoplasmic Reticulum 0.0296 0.0106 0.0114 0.0229 0.0132 0.0163 – – – – – – 0.0107 0.0003 0.001 0.0005 0.0008 0.0256
Endosome 0.02 0.0026 0.018 0.0153 0.0899 0.036 – – – – – – 0.0085 0.0006 0.0036 0.0019 0.001 0.024
Golgi 0.0064 0.0012 0.0073 0.0007 0.0091 0.0207 – – – – – – 0.0019 0.0001 0.0026 0.0001 0.0001 0.0139
Lipid Particles 0.0098 0.0027 0.0034 0.001 0.0027 0.0021 – – – – – – 0.0001 0 0.0005 0.0001 0.0001 0.0003
Mitochondria 0.0103 0.0058 0.0456 0.001 0.006 0.0675 – – – – – – 0.0039 0.0001 0.0986 0.0011 0.0009 0.1136
None 0.0113 0.0018 0.0071 0.0021 0.0026 0.0018 – – – – – – 0.0036 0.0001 0.0016 0.0007 0.0003 0.0021
Nuclear Periphery 0.04 0.0041 0.0089 0.0052 0.0068 0.0328 – – – – – – 0.0321 0.0005 0.005 0.0062 0.0071 0.0125
Nucleolus 0.0133 0.0017 0.0038 0.0005 0.0028 0.0014 – – – – – – 0.001 0.0001 0.0023 0.0003 0.0005 0.0001
Nucleus 0.0257 0.0033 0.0107 0.0044 0.0088 0.0145 – – – – – – 0.1196 0.0008 0.0091 0.0076 0.0171 0.0051
Peroxisomes 0.002 0.0002 0.0003 0.0001 0.0005 0.0005 – – – – – – 0 0 0.0008 0.0001 0.0002 0.0004
Punctate Nuclear 0.0033 0.0003 0.0004 0.0001 0.0006 0.0005 – – – – – – 0.0002 0 0.0001 0.0001 0.0001 0.0004
Vacuole 0.0749 0.0411 0.0711 0.0678 0.0816 0.0691 – – – – – – 0.0184 0.007 0.0353 0.0362 0.0213 0.0176
Vacuole Periphery 0.0256 0.004 0.0151 0.0184 0.0191 0.0438 – – – – – – 0.0097 0.001 0.0151 0.022 0.0058 0.015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2097.7367 2047.5809 2269.8357 2095.5414 2399.1529 1837.9893 1811.232 1623.2405 1909.8991 1769.0447
Translational Efficiency 1.5351 1.6241 1.5303 1.5174 1.5375 1.7672 1.5523 1.8156 1.5369 1.776

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
9 69 370 8 53 16 1428 1 62 85 1798 9

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4901.69 306.06 6073.15 355.50 4305.80 293.02 6353.18 287.75 4392.30 303.61 6295.55 347.97
Standard Deviation 1100.95 4.89 1512.59 8.36 1179.27 11.20 1420.98 — 1186.94 8.31 1444.75 22.70
Intensity Change Log 2 — -4.001393 0.309166 -3.785358 — -3.877210 0.561198 -3.903393 — -3.941987 0.432520 -3.839356

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000343 0.000030 0.000231 0.000693 0.000374 0.000074 0.000177 0.000026 0.000369 0.000038 0.000188 0.000619
Bud Neck 0.000618 0.000013 0.001471 0.000031 0.001588 0.000019 0.001233 0.000000 0.001447 0.000014 0.001282 0.000027
Bud Site 0.016460 0.000033 0.012701 0.000040 0.019702 0.000021 0.008104 0.000001 0.019231 0.000031 0.009050 0.000036
Cell Periphery 0.009263 0.000076 0.044208 0.001548 0.038878 0.000093 0.009955 0.000009 0.034579 0.000079 0.017004 0.001377
Cytoplasm 0.598276* 0.000053 0.545687* 0.000183 0.451648* 0.000046 0.794370* 0.000002 0.472933* 0.000052 0.743195* 0.000163
Cytoplasmic Foci 0.009999 0.000016 0.014600 0.000030 0.039944 0.000026 0.012249 0.000003 0.035597 0.000018 0.012733 0.000027
Eisosomes 0.000004 0.000095 0.000005 0.008562 0.000010 0.000147 0.000002 0.000024 0.000009 0.000105 0.000003 0.007613
Endoplasmic Reticulum 0.000864 0.000001 0.000565 0.000004 0.000850 0.000001 0.000367 0.000000 0.000852 0.000001 0.000408 0.000004
Endosome 0.002278 0.000002 0.000964 0.000001 0.001188 0.000002 0.000804 0.000000 0.001346 0.000002 0.000837 0.000001
Golgi 0.046593 0.000006 0.055617 0.000015 0.157689* 0.000009 0.028430 0.000000 0.141562* 0.000007 0.034025 0.000014
Lipid Particles 0.004037 0.000029 0.020223 0.000372 0.010346 0.000092 0.009091 0.000001 0.009430 0.000040 0.011382 0.000331
Mitochondria 0.000168 0.000012 0.000343 0.000032 0.000594 0.000013 0.000627 0.000001 0.000533 0.000012 0.000569 0.000029
Mitotic Spindle 0.001763 0.000280 0.001231 0.000806 0.002411 0.000602 0.001435 0.000074 0.002317 0.000340 0.001393 0.000725
None 0.031323 0.999157* 0.003046 0.982388* 0.001892 0.998554* 0.003322 0.999833* 0.006164 0.999043* 0.003265 0.984327*
Nuclear Periphery 0.000119 0.000000 0.000915 0.000001 0.001083 0.000000 0.000394 0.000000 0.000943 0.000000 0.000501 0.000001
Nuclear Periphery Foci 0.005289 0.000064 0.008175 0.000001 0.009265 0.000035 0.006399 0.000000 0.008687 0.000058 0.006764 0.000001
Nucleolus 0.000220 0.000049 0.000189 0.000002 0.003525 0.000032 0.000148 0.000000 0.003045 0.000046 0.000157 0.000002
Nucleus 0.035675 0.000014 0.013961 0.000015 0.011947 0.000014 0.018052 0.000001 0.015391 0.000014 0.017210 0.000014
Peroxisomes 0.000044 0.000070 0.000080 0.005274 0.000165 0.000218 0.000074 0.000025 0.000147 0.000098 0.000075 0.004691
Vacuole 0.110099 0.000002 0.167653 0.000000 0.079367 0.000001 0.072824 0.000000 0.083828 0.000002 0.092339 0.000000
Vacuole Periphery 0.126567* 0.000000 0.108134* 0.000000 0.167536* 0.000000 0.031940 0.000000 0.161589* 0.000000 0.047620 0.000000

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps19b

Rps19b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps19b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available