Standard name
Human Ortholog
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.06 0.25 0.51 0.49 0.54 0.32 0.36 0.24 0.33 – 0.22 0.3 0.15 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.16 0 0 0 0 0 0 0 – 0 0.09 0.05 0.05 0.05 0.07 0.05 0.05 0 0.08 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0 0.08 0.1 0 0.17 0.3 0.26 0.59 0.46 0.74 0.54 – 0 0 0.2 0 0.08 0 0 0 0.06 0 0
Nucleus 0.63 0.73 0.96 0.63 0.27 0.14 0.12 0.13 0.17 0.12 0.1 0.12 – 0.38 0.35 0.42 0.53 0.44 0.31 0.44 0.43 0.49 0.31 0.19
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0.27 0.14 0 0.27 0 0 0 0 0 0 0 0 – 0.28 0.14 0.31 0.32 0.22 0.41 0.3 0.22 0.31 0.26 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.26 0.22 0.08 0.09 0.13 0 0.08 0.07 0.14 – 0.25 0.25 0 0 0 0.1 0.07 0.08 0 0.07 0.13
Vac/Vac Membrane 0 0.08 0 0 0.17 0.17 0.05 0 0.05 0.06 0 0.06 – 0 0.05 0.09 0.32 0.23 0 0.09 0.13 0 0.17 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Bud 2 0 0 1 0 1 6 10 3 11 12 13 – 0 0 0 0 0 0 3 1 0 1 1
Bud Neck 0 0 0 0 2 3 5 18 5 10 4 5 – 1 0 0 0 0 1 1 0 0 1 2
Bud Site 0 0 0 0 0 0 2 3 3 5 5 6 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 12 0 6 44 44 95 140 51 83 59 94 – 15 31 8 1 10 0 3 1 0 2 2
Endoplasmic Reticulum 2 0 0 0 0 0 1 0 0 1 1 1 – 1 0 3 1 4 0 0 0 0 0 0
Endosome 1 11 0 4 28 2 3 5 1 7 0 5 – 2 9 3 1 6 14 14 3 0 7 15
Golgi 0 1 0 1 1 1 0 0 0 0 0 0 – 0 0 1 0 0 1 1 0 0 0 0
Mitochondria 74 10 2 10 4 15 58 67 93 105 179 153 – 2 1 11 0 9 4 4 2 1 2 5
Nucleus 116 194 23 61 49 12 23 33 26 28 23 33 – 26 36 23 10 49 64 139 30 14 27 25
Nuclear Periphery 2 0 0 0 0 0 0 0 0 0 1 1 – 0 0 0 1 1 2 0 0 1 0 1
Nucleolus 50 36 0 26 2 0 0 1 0 0 1 3 – 19 14 17 6 24 85 96 15 8 22 32
Peroxisomes 0 0 0 0 0 0 2 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 11 0 25 39 7 18 33 5 19 18 39 – 17 26 2 0 4 20 21 5 0 5 16
Vac/Vac Membrane 2 20 1 0 31 15 9 10 8 14 6 16 – 1 5 5 6 25 8 27 9 0 15 26
Unique Cell Count 185 264 24 97 179 87 192 261 157 229 242 285 69 102 55 19 111 208 317 71 29 89 135
Labelled Cell Count 256 295 26 134 200 100 222 322 195 284 309 369 84 122 73 26 132 208 317 71 29 89 135


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.7 5.2 6.1 5.7 4.6 4.4 4.5 3.6 3.9 3.4 3.7 – 5.9 5.4 8.0 9.2 8.3 – 6.4 6.3
Std Deviation (1e-4) 0.7 1.0 0.5 1.3 1.3 1.2 1.0 1.1 0.7 1.4 1.2 0.7 – 1.3 1.0 2.1 3.0 2.1 – 1.2 1.5
Intensity Change (Log2) – – – 0.24 0.15 -0.17 -0.25 -0.2 -0.51 -0.4 -0.59 -0.49 – 0.18 0.05 0.62 0.82 0.67 – 0.3 0.28

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 2.7 4.5 4.6 5.0 3.3 3.6 2.7 3.4 – 0 3.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus -3.1 -6.6 -7.7 -9.5 0 -8.1 0 0 0 – -4.9 -5.3 -4.5 -3.3 -4.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 2.9 0 0 0 0 0 0 0 0 – 0 0 3.1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.173 0.3626 0.3053 -0.3344 0.0552 0.2593 0.5787 1.1717 0.8377 0.8912 1.0225 1.0669 -0.5831 -0.2065 -0.5192 -0.3872 0.8711 -0.4243
Actin 0.0335 0 0.0085 0.0001 0.0021 0.0007 0.0116 0 0.0067 0.0111 0.0168 0.0011 0.0118 0 0.0186 0.0135 0.0278 0.0033
Bud 0.0006 0.0001 0.0004 0.0002 0.0007 0.0003 0.0004 0.0001 0.0003 0.0002 0.0011 0.0002 0.0005 0.0002 0.0008 0.0007 0.0016 0.0003
Bud Neck 0.0051 0.002 0.0028 0.0036 0.0055 0.0071 0.0047 0.0046 0.0024 0.0028 0.0062 0.0052 0.0045 0.0021 0.0022 0.0031 0.0025 0.0059
Bud Periphery 0.0015 0.0002 0.001 0.0005 0.0012 0.001 0.0012 0.0002 0.0008 0.0003 0.0048 0.0007 0.0013 0.0005 0.0011 0.0025 0.0017 0.0007
Bud Site 0.0042 0.0025 0.0031 0.0021 0.0035 0.0013 0.0015 0.002 0.0057 0.0015 0.0026 0.002 0.0067 0.006 0.0094 0.0024 0.0036 0.0014
Cell Periphery 0.0008 0.0003 0.0007 0.0005 0.0005 0.0003 0.0004 0.0006 0.0005 0.0003 0.0007 0.0004 0.0009 0.0006 0.0006 0.0008 0.0004 0.0007
Cytoplasm 0.0068 0.0013 0.0012 0.0052 0.0039 0.0024 0.0054 0.0031 0.0005 0.0006 0.0025 0.0005 0.0209 0.0011 0.0016 0.0004 0.0149 0.001
Cytoplasmic Foci 0.0202 0.0012 0.0058 0.0039 0.013 0.0053 0.0112 0.0074 0.0067 0.026 0.0057 0.0066 0.0227 0.0061 0.0072 0.002 0.0477 0.013
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0016 0 0.0004 0 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0023 0.0004 0.0008 0.0011 0.0013 0.0015 0.0048 0.0001 0.0002 0.0003 0.0026 0.0002 0.0037 0.0004 0.001 0.0011 0.0033 0.0004
Endosome 0.0217 0.0025 0.0084 0.0142 0.037 0.0119 0.0263 0.0031 0.0108 0.0106 0.04 0.0107 0.0194 0.0059 0.0139 0.0083 0.0856 0.0061
Golgi 0.0092 0.0003 0.0019 0.0008 0.0115 0.0015 0.0041 0.0005 0.0057 0.0139 0.0265 0.0099 0.0064 0.0006 0.009 0.0057 0.0229 0.0036
Lipid Particles 0.0431 0.0043 0.0128 0.0179 0.0449 0.0184 0.0377 0.0047 0.0124 0.0402 0.0165 0.0116 0.0338 0.0147 0.0226 0.0127 0.0602 0.0297
Mitochondria 0.007 0.0002 0.0152 0.0007 0.0252 0.0181 0.0041 0.0002 0.0086 0.0043 0.0597 0.0042 0.0067 0.0006 0.0203 0.0027 0.0088 0.0123
None 0.0106 0.0129 0.0041 0.0042 0.0022 0.0192 0.0184 0.0007 0.0006 0.0007 0.0259 0.0006 0.0028 0.0009 0.0132 0.0004 0.0024 0.0097
Nuclear Periphery 0.0223 0.0081 0.0112 0.0073 0.0799 0.015 0.0283 0.0014 0.0012 0.0018 0.0031 0.0041 0.0105 0.0172 0.0067 0.0091 0.044 0.0037
Nucleolus 0.4285 0.5728 0.5911 0.668 0.3771 0.5129 0.5035 0.6708 0.6581 0.63 0.4757 0.568 0.5054 0.5865 0.6293 0.5359 0.3368 0.5553
Nucleus 0.2613 0.3296 0.2698 0.1941 0.2959 0.32 0.2614 0.2667 0.2525 0.204 0.2628 0.2788 0.3013 0.3012 0.2021 0.371 0.2302 0.2737
Peroxisomes 0.0096 0.0002 0.0018 0.001 0.0042 0.0027 0.0085 0.0005 0.0072 0.019 0.0029 0.0101 0.0065 0.0015 0.0115 0.0013 0.0185 0.0162
Punctate Nuclear 0.099 0.0573 0.0483 0.0473 0.0736 0.0512 0.0539 0.0264 0.0112 0.0296 0.0376 0.0781 0.0209 0.0485 0.0227 0.0078 0.0818 0.058
Vacuole 0.0095 0.0032 0.009 0.0256 0.0118 0.007 0.0088 0.0064 0.0064 0.0022 0.0032 0.0061 0.0097 0.0044 0.0043 0.0156 0.0031 0.0043
Vacuole Periphery 0.0029 0.0005 0.0022 0.0018 0.0047 0.0022 0.0037 0.0003 0.0013 0.0005 0.0015 0.0009 0.0034 0.0008 0.0014 0.0031 0.0021 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7343 14.8289 12.142 11.6398 15.2035 13.1489 13.3132 8.7238 10.8379 13.2591
Translational Efficiency 0.4167 0.9758 0.99 0.7883 0.5472 0.403 0.6836 0.7943 0.6275 0.626

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
377 1859 144 134 1643 2171 142 751 2020 4030 286 885

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 612.98 742.88 1102.08 1126.60 723.57 783.59 1058.72 1003.77 702.93 764.81 1080.55 1022.37
Standard Deviation 62.76 125.24 123.23 146.85 99.80 102.19 127.44 145.55 103.41 115.21 127.20 152.26
Intensity Change Log 2 — 0.277289 0.846317 0.878063 — 0.114966 0.549117 0.472224 — 0.191685 0.693052 0.672590

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000094 0.000378 0.002124 0.000833 0.000271 0.000210 0.001093 0.000758 0.000238 0.000287 0.001612 0.000769
Bud Neck 0.002991 0.009943 0.018145 0.033297 0.005841 0.006455 0.011200 0.033690 0.005310 0.008064 0.014697 0.033631
Bud Site 0.000300 0.001262 0.007380 0.003547 0.001442 0.000742 0.004585 0.006060 0.001229 0.000982 0.005992 0.005679
Cell Periphery 0.000080 0.000103 0.000102 0.000052 0.000091 0.000038 0.000055 0.000090 0.000089 0.000068 0.000079 0.000084
Cytoplasm 0.023480 0.028671 0.002745 0.001738 0.024828 0.004450 0.001033 0.008230 0.024576 0.015623 0.001895 0.007247
Cytoplasmic Foci 0.007651 0.003994 0.000437 0.000159 0.008157 0.001689 0.000543 0.004330 0.008063 0.002753 0.000490 0.003698
Eisosomes 0.000048 0.000037 0.000101 0.000060 0.000058 0.000034 0.000073 0.000049 0.000056 0.000035 0.000087 0.000051
Endoplasmic Reticulum 0.001109 0.003171 0.008790 0.002704 0.002457 0.002388 0.005443 0.002291 0.002206 0.002749 0.007128 0.002354
Endosome 0.000869 0.001927 0.001378 0.001079 0.003120 0.002071 0.001234 0.003788 0.002700 0.002005 0.001306 0.003378
Golgi 0.000269 0.000703 0.000793 0.000520 0.000984 0.001096 0.000889 0.002846 0.000851 0.000915 0.000841 0.002493
Lipid Particles 0.003853 0.001472 0.001103 0.000176 0.004681 0.001089 0.000508 0.000370 0.004527 0.001265 0.000808 0.000341
Mitochondria 0.002240 0.003530 0.001140 0.001705 0.004419 0.007307 0.001283 0.004314 0.004012 0.005565 0.001211 0.003919
Mitotic Spindle 0.000140 0.004336 0.007278 0.019398 0.004994 0.007907 0.008504 0.028956 0.004088 0.006260 0.007886 0.027508
None 0.026977 0.017803 0.001241 0.001494 0.013371 0.003959 0.001304 0.004351 0.015910 0.010345 0.001272 0.003919
Nuclear Periphery 0.003102 0.002071 0.000989 0.001118 0.003207 0.004263 0.000801 0.002085 0.003187 0.003252 0.000895 0.001939
Nuclear Periphery Foci 0.006086 0.003364 0.010089 0.001989 0.008541 0.003495 0.003924 0.001803 0.008082 0.003435 0.007028 0.001831
Nucleolus 0.359065 0.260443 0.413945 0.502223 0.390367 0.318530 0.400300 0.394279 0.384525 0.291735 0.407170 0.410623
Nucleus 0.559757 0.652854 0.507420 0.417630 0.517714 0.629893 0.540373 0.492979 0.525561 0.640485 0.523781 0.481570
Peroxisomes 0.001271 0.001127 0.005056 0.003928 0.002349 0.001087 0.002783 0.001477 0.002148 0.001106 0.003927 0.001848
Vacuole 0.000333 0.002073 0.009036 0.004848 0.002170 0.002002 0.011994 0.004804 0.001827 0.002035 0.010505 0.004810
Vacuole Periphery 0.000283 0.000737 0.000709 0.001500 0.000936 0.001295 0.002078 0.002452 0.000814 0.001038 0.001388 0.002308

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.05 -8.78 -9.13 -6.75 4.68 2.40 -7.08 -12.13 -14.31 3.26 -1.32 -10.69 -14.74 -14.73 6.18
Bud Neck -9.34 -9.26 -8.88 -7.25 -3.30 -1.04 -5.66 -16.25 -16.15 -8.83 -4.97 -9.95 -18.25 -17.10 -8.79
Bud Site -5.57 -2.95 -4.67 -4.06 0.43 1.71 -2.55 -4.45 -5.18 -0.36 0.66 -3.63 -5.42 -5.82 0.30
Cell Periphery -1.57 -3.02 -0.30 1.21 2.77 6.66 0.89 0.19 -3.44 -0.48 3.16 -1.61 0.11 -1.70 1.29
Cytoplasm -1.29 4.46 4.53 9.50 0.15 9.05 11.14 5.54 -1.98 -3.80 4.04 10.90 6.53 3.80 -3.01
Cytoplasmic Foci 1.98 3.70 3.82 7.14 1.76 7.29 9.32 5.35 -0.64 -2.35 6.76 10.07 6.21 0.96 -2.31
Eisosomes 3.01 -5.76 -2.82 -6.06 4.23 10.06 -3.47 3.20 -6.34 4.56 10.45 -6.23 1.99 -7.84 6.98
Endoplasmic Reticulum -8.33 -7.89 -5.05 -0.58 5.48 0.36 -5.36 -3.27 -3.54 4.14 -3.07 -8.81 -4.79 -2.61 6.94
Endosome -3.07 -3.47 -1.36 0.89 1.18 2.53 2.75 0.67 -0.88 -1.25 2.17 2.26 0.40 -0.85 -0.98
Golgi -3.74 -7.46 -5.74 -0.06 2.98 0.04 0.29 -1.16 -1.10 -1.22 0.08 -0.74 -1.33 -1.34 -1.08
Lipid Particles 2.76 2.42 4.16 5.82 3.55 6.71 6.85 8.16 4.54 2.07 7.08 6.75 9.04 6.75 3.95
Mitochondria -2.93 2.73 0.59 3.98 -3.01 -2.51 5.16 0.60 2.83 -3.56 -2.10 5.61 0.55 2.39 -3.82
Mitotic Spindle -4.57 -5.67 -3.07 -2.59 -1.63 -1.58 -1.66 -6.18 -5.48 -2.94 -1.70 -3.21 -6.94 -6.43 -3.94
None 2.19 6.42 6.11 10.64 -1.49 8.26 10.94 6.98 -0.06 -2.86 4.48 12.64 9.02 6.48 -3.18
Nuclear Periphery 1.26 2.22 1.75 1.12 -0.66 -1.35 3.59 0.53 1.68 -2.16 -0.19 3.93 0.80 1.04 -2.06
Nuclear Periphery Foci 1.56 -2.02 0.42 -0.41 1.90 5.11 1.81 7.24 3.38 2.95 5.52 -0.05 6.52 2.52 4.88
Nucleolus 6.13 -1.52 -3.53 -8.33 -1.79 8.04 0.70 3.20 -2.76 0.94 12.07 -0.14 1.64 -7.12 1.16
Nucleus -5.73 2.57 5.39 10.64 2.41 -12.80 -1.18 -0.38 9.60 0.94 -15.28 0.31 1.62 13.33 0.72
Peroxisomes 0.57 -6.28 -4.84 -5.92 1.54 4.46 -1.17 3.08 -2.03 3.52 4.65 -4.31 1.41 -4.49 5.87
Vacuole -4.15 -4.77 -2.36 -2.08 -0.24 0.30 -2.04 -4.20 -4.27 1.03 -0.76 -3.54 -4.84 -4.67 1.25
Vacuole Periphery -4.64 -6.12 -6.09 -3.59 -2.53 -1.36 -0.79 -2.59 -1.60 -0.18 -1.22 -1.00 -3.21 -2.64 -0.90
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
Localization
Cell Percentages nucleus (41%), nucleolus (30%), mixed (25%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Trf5

Trf5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trf5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available