Standard name | |
---|---|
Human Ortholog | |
Description | Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 11 | 8 | 1 | 0 | 3 | 2 | 2 | 3 | – | 3 | 2 | 2 | 6 | 5 | 2 | 2 | 2 | 8 | 10 | 5 | 4 | 11 | 9 |
Bud | 6 | 6 | 20 | 16 | 40 | 27 | 50 | 56 | 56 | – | 52 | 68 | 6 | 4 | 12 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 9 | 10 | 25 | – | 15 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 1 | 1 | 1 | 0 | 2 | 8 | 0 | – | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 15 | 2 | 16 | 18 | 11 | 27 | 25 | 13 | – | 12 | 10 | 6 | 10 | 11 | 3 | 12 | 3 | 0 | 0 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 2 | 12 | 6 | 8 | 2 | 5 | 7 | 8 | 3 | – | 1 | 8 | 4 | 12 | 6 | 1 | 0 | 2 | 0 | 1 | 1 | 2 | 2 | 0 |
Endosome | 43 | 15 | 51 | 31 | 160 | 36 | 54 | 92 | 14 | – | 12 | 16 | 18 | 39 | 22 | 20 | 9 | 5 | 47 | 80 | 108 | 38 | 55 | 45 |
Golgi | 94 | 53 | 34 | 6 | 3 | 1 | 0 | 0 | 0 | – | 0 | 0 | 69 | 89 | 24 | 10 | 13 | 6 | 144 | 176 | 175 | 76 | 148 | 140 |
Mitochondria | 80 | 234 | 304 | 299 | 363 | 485 | 713 | 833 | 742 | – | 493 | 704 | 147 | 168 | 225 | 0 | 4 | 2 | 1 | 26 | 24 | 12 | 15 | 6 |
Nucleus | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 1 | 2 | – | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 4 | 3 | 2 | 2 | 7 | 11 | 6 | 9 | – | 4 | 15 | 4 | 2 | 8 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 5 | 2 | 3 | 3 | 4 | 11 | 13 | 28 | 24 | – | 21 | 26 | 0 | 1 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 6 | 26 | 16 | 171 | 47 | 92 | 134 | 49 | – | 37 | 54 | 0 | 4 | 2 | 0 | 1 | 0 | 12 | 12 | 54 | 4 | 11 | 21 |
SpindlePole | 5 | 2 | 5 | 4 | 47 | 19 | 59 | 83 | 54 | – | 32 | 34 | 2 | 0 | 3 | 5 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 15 | 19 | 17 | 5 | 8 | 10 | 14 | 20 | 9 | – | 8 | 4 | 7 | 18 | 11 | 5 | 6 | 2 | 1 | 9 | 4 | 1 | 0 | 1 |
Unique Cell Count | 183 | 287 | 349 | 347 | 526 | 542 | 821 | 1016 | 797 | 548 | 768 | 183 | 248 | 270 | 39 | 42 | 18 | 221 | 325 | 380 | 144 | 249 | 230 | |
Labelled Cell Count | 267 | 382 | 481 | 409 | 819 | 665 | 1056 | 1306 | 1003 | 693 | 964 | 265 | 353 | 334 | 50 | 53 | 22 | 221 | 325 | 380 | 144 | 249 | 230 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 7.4 | 6.0 | 6.0 | 6.1 | 5.5 | 5.2 | 5.3 | 4.7 | – | 4.7 | 4.5 | 7.4 | 7.6 | 6.6 | 8.5 | 10.2 | 9.7 | 8.0 | 7.8 | 7.8 |
Std Deviation (1e-4) | 1.3 | 1.6 | 0.8 | 1.2 | 1.2 | 1.1 | 1.4 | 1.3 | 1.0 | – | 1.4 | 1.4 | 1.1 | 1.2 | 1.0 | 2.1 | 2.7 | 1.4 | 1.3 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.03 | -0.11 | -0.21 | -0.19 | -0.35 | – | -0.36 | -0.41 | 0.31 | 0.35 | 0.13 | 0.51 | 0.77 | 0.7 | 0.42 | 0.39 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.1 | -0.4 | 0 | 0 | 0 |
Bud | -0.7 | 1.1 | -0.5 | 0.2 | -0.2 | 0 | – | 0 | 0 | -1.2 | -2.5 | -0.7 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.4 | 2.8 | 1.8 | 2.7 | 2.2 | 0 | – | 1.9 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.6 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.2 | 0.5 | 0 | 0 | 0 |
Endosome | -2.3 | 5.4 | -3.9 | -4.4 | -2.9 | 0 | – | 0 | 0 | -1.6 | 0.4 | -2.5 | 5.6 | 1.2 | 0 |
Golgi | -4.5 | -6.6 | -7.2 | -9.1 | -10.1 | 0 | – | 0 | 0 | 7.8 | 7.8 | -0.4 | 0 | 4.0 | 0 |
Mitochondria | -0.4 | -6.2 | 1.1 | -0.1 | -2.2 | 0 | – | 0 | 0 | -2.1 | -5.7 | -1.3 | -12.5 | -11.6 | -8.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.4 | 0 | 2.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.6 | 8.7 | 0.6 | 2.0 | 2.9 | 0 | – | 0 | 0 | -3.8 | -3.2 | -4.0 | 0 | 0 | 0 |
SpindlePole | 0 | 4.6 | 1.9 | 4.0 | 4.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | -2.9 | -2.6 | -3.1 | -2.9 | -3.9 | – | -3.0 | -5.0 | -0.6 | 1.2 | -0.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.4916 | 8.9738 | 7.9816 | 7.1926 | 7.3944 | 8.5176 | 5.1094 | 7.5786 | 6.4886 | 5.9745 | 5.3652 | 6.7797 | 6.9995 | 6.8698 | 6.8273 | 7.1793 | 6.3333 | 7.1517 |
Actin | 0.1976 | 0.0271 | 0.0179 | 0.0525 | 0.0606 | 0.0185 | 0.0492 | 0.0127 | 0.0224 | 0.0318 | 0.0421 | 0.012 | 0.0398 | 0.042 | 0.0083 | 0.0013 | 0.0049 | 0.0162 |
Bud | 0.001 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0042 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.004 | 0.0004 | 0.0004 | 0.0009 | 0.0005 | 0.001 | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0003 |
Bud Periphery | 0.0016 | 0.0002 | 0.0002 | 0.0008 | 0.0003 | 0.0006 | 0.0003 | 0.0015 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0086 | 0.0004 | 0.0005 | 0.0026 | 0.0004 | 0.0005 | 0.0009 | 0.0043 | 0.0004 | 0.0014 | 0.001 | 0.0001 | 0.0005 | 0.0002 | 0.0016 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0079 | 0.0008 | 0.001 | 0.0007 | 0.015 | 0.0009 | 0.0057 | 0.0019 | 0.0008 | 0.0008 | 0.0007 | 0.0008 | 0.0022 | 0.0006 | 0.0006 | 0.0007 | 0.0011 | 0.0005 |
Cytoplasmic Foci | 0.0657 | 0.1071 | 0.0938 | 0.0941 | 0.0784 | 0.0489 | 0.1087 | 0.1078 | 0.0758 | 0.0711 | 0.0671 | 0.0589 | 0.0926 | 0.1233 | 0.1039 | 0.079 | 0.1046 | 0.0648 |
Eisosomes | 0.0017 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0087 | 0.0003 | 0.0011 | 0.0012 | 0.0019 | 0.0084 | 0.0038 | 0.0003 | 0.0007 | 0.0007 | 0.001 | 0.0005 | 0.005 | 0.0002 | 0.0002 | 0.0004 | 0.0008 | 0.0004 |
Endosome | 0.1405 | 0.1622 | 0.1768 | 0.1833 | 0.1924 | 0.1816 | 0.193 | 0.1944 | 0.1653 | 0.2287 | 0.248 | 0.1645 | 0.1653 | 0.1409 | 0.1285 | 0.2472 | 0.22 | 0.1315 |
Golgi | 0.3536 | 0.5638 | 0.5689 | 0.5525 | 0.3712 | 0.5714 | 0.4273 | 0.5893 | 0.6078 | 0.5791 | 0.4632 | 0.6842 | 0.5009 | 0.5872 | 0.6527 | 0.6031 | 0.5686 | 0.6719 |
Lipid Particles | 0.0962 | 0.0762 | 0.0696 | 0.0536 | 0.1381 | 0.0802 | 0.1129 | 0.0171 | 0.0285 | 0.0225 | 0.0563 | 0.0399 | 0.0763 | 0.0457 | 0.0298 | 0.0536 | 0.0261 | 0.0331 |
Mitochondria | 0.0249 | 0.0253 | 0.0318 | 0.0306 | 0.014 | 0.0398 | 0.0215 | 0.0211 | 0.031 | 0.0123 | 0.0262 | 0.0132 | 0.0256 | 0.032 | 0.0208 | 0.0027 | 0.0095 | 0.0123 |
None | 0.0062 | 0 | 0.0004 | 0.0002 | 0.0013 | 0.0004 | 0.0091 | 0.0126 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0021 | 0 | 0.0002 | 0 | 0.0004 | 0 |
Nuclear Periphery | 0.017 | 0.0001 | 0.0007 | 0.0008 | 0.0135 | 0.0153 | 0.0031 | 0.0001 | 0.0017 | 0.0004 | 0.0075 | 0.0003 | 0.011 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Nucleolus | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0096 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Nucleus | 0.0149 | 0 | 0.0001 | 0.0002 | 0.0477 | 0.0007 | 0.0012 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.0309 | 0.0337 | 0.0282 | 0.021 | 0.053 | 0.0193 | 0.0496 | 0.0289 | 0.0465 | 0.0446 | 0.0697 | 0.0179 | 0.0688 | 0.0261 | 0.0496 | 0.0069 | 0.048 | 0.0667 |
Punctate Nuclear | 0.0125 | 0.0002 | 0.0007 | 0.0008 | 0.0027 | 0.0009 | 0.0042 | 0.0002 | 0.0045 | 0.0008 | 0.0009 | 0.0002 | 0.0027 | 0.0001 | 0.0007 | 0.0001 | 0.0118 | 0.0001 |
Vacuole | 0.0023 | 0.0006 | 0.0029 | 0.0012 | 0.0026 | 0.0036 | 0.0044 | 0.0011 | 0.0012 | 0.0013 | 0.0033 | 0.0019 | 0.0021 | 0.0004 | 0.0006 | 0.0014 | 0.0013 | 0.0005 |
Vacuole Periphery | 0.0029 | 0.0012 | 0.0044 | 0.0022 | 0.0057 | 0.0071 | 0.0032 | 0.0015 | 0.0021 | 0.0022 | 0.0101 | 0.004 | 0.0026 | 0.0007 | 0.001 | 0.0031 | 0.0019 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.5012 | 18.8799 | 23.5483 | 33.0235 | 25.6351 | 19.3118 | 28.1265 | 27.5501 | 29.0838 | 34.7484 |
Translational Efficiency | 0.4242 | 0.5253 | 0.4839 | 0.3968 | 0.4223 | 0.5717 | 0.3875 | 0.3331 | 0.4484 | 0.3623 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
920 | 1390 | 132 | 137 | 1558 | 1504 | 146 | 3223 | 2478 | 2894 | 278 | 3360 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 746.59 | 945.65 | 1343.03 | 1030.14 | 727.70 | 888.27 | 1127.61 | 1002.32 | 734.71 | 915.83 | 1229.90 | 1003.45 |
Standard Deviation | 145.43 | 136.02 | 145.68 | 153.54 | 120.43 | 120.22 | 130.14 | 127.66 | 130.59 | 131.22 | 174.77 | 128.93 |
Intensity Change Log 2 | — | 0.340990 | 0.847103 | 0.464452 | — | 0.287654 | 0.631852 | 0.461927 | — | 0.314910 | 0.744864 | 0.463207 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015557 | 0.002360 | 0.012670 | 0.002643 | 0.003104 | 0.002063 | 0.002622 | 0.002624 | 0.007727 | 0.002206 | 0.007393 | 0.002624 |
Bud Neck | 0.000798 | 0.000069 | 0.002854 | 0.000390 | 0.000521 | 0.000113 | 0.001299 | 0.000113 | 0.000624 | 0.000092 | 0.002037 | 0.000125 |
Bud Site | 0.003692 | 0.000435 | 0.006564 | 0.001144 | 0.000702 | 0.000449 | 0.002446 | 0.000906 | 0.001812 | 0.000442 | 0.004401 | 0.000915 |
Cell Periphery | 0.000268 | 0.000027 | 0.000120 | 0.000047 | 0.000085 | 0.000035 | 0.000052 | 0.000030 | 0.000153 | 0.000031 | 0.000084 | 0.000031 |
Cytoplasm | 0.001513 | 0.000291 | 0.001288 | 0.000421 | 0.000519 | 0.000536 | 0.000651 | 0.000783 | 0.000888 | 0.000418 | 0.000953 | 0.000768 |
Cytoplasmic Foci | 0.158702 | 0.277947* | 0.315048* | 0.342881* | 0.181926* | 0.249402* | 0.344251* | 0.200304* | 0.173304 | 0.263113* | 0.330385* | 0.206118* |
Eisosomes | 0.000219 | 0.000032 | 0.000049 | 0.000027 | 0.000063 | 0.000034 | 0.000035 | 0.000028 | 0.000121 | 0.000033 | 0.000042 | 0.000028 |
Endoplasmic Reticulum | 0.001402 | 0.000207 | 0.002428 | 0.000771 | 0.000338 | 0.000134 | 0.000547 | 0.000183 | 0.000733 | 0.000169 | 0.001440 | 0.000207 |
Endosome | 0.261841* | 0.222487* | 0.243913* | 0.362340* | 0.268288* | 0.241301* | 0.305355* | 0.377092* | 0.265894* | 0.232264* | 0.276181* | 0.376491* |
Golgi | 0.444467* | 0.461063* | 0.251957* | 0.220023* | 0.462037* | 0.468528* | 0.219543* | 0.361506* | 0.455514* | 0.464943* | 0.234934* | 0.355738* |
Lipid Particles | 0.026477 | 0.011206 | 0.014618 | 0.017175 | 0.042682 | 0.010684 | 0.037460 | 0.007955 | 0.036666 | 0.010935 | 0.026614 | 0.008331 |
Mitochondria | 0.056706 | 0.010432 | 0.001088 | 0.005909 | 0.019254 | 0.013500 | 0.002809 | 0.016370 | 0.033159 | 0.012026 | 0.001992 | 0.015944 |
Mitotic Spindle | 0.000560 | 0.001794 | 0.040470 | 0.004865 | 0.000559 | 0.001129 | 0.017011 | 0.003859 | 0.000560 | 0.001448 | 0.028150 | 0.003900 |
None | 0.000946 | 0.000013 | 0.000010 | 0.000007 | 0.000805 | 0.000046 | 0.000012 | 0.000014 | 0.000857 | 0.000030 | 0.000011 | 0.000014 |
Nuclear Periphery | 0.000351 | 0.000044 | 0.002063 | 0.002168 | 0.000028 | 0.000024 | 0.000397 | 0.000158 | 0.000148 | 0.000034 | 0.001188 | 0.000240 |
Nuclear Periphery Foci | 0.000746 | 0.000426 | 0.015693 | 0.004845 | 0.000856 | 0.000244 | 0.002596 | 0.000491 | 0.000815 | 0.000331 | 0.008815 | 0.000669 |
Nucleolus | 0.001171 | 0.000042 | 0.000364 | 0.000038 | 0.001009 | 0.000015 | 0.000178 | 0.000006 | 0.001069 | 0.000028 | 0.000266 | 0.000007 |
Nucleus | 0.002784 | 0.000037 | 0.000315 | 0.000205 | 0.000858 | 0.000057 | 0.000171 | 0.000018 | 0.001573 | 0.000048 | 0.000239 | 0.000026 |
Peroxisomes | 0.014067 | 0.006178 | 0.011671 | 0.010233 | 0.011940 | 0.005984 | 0.024846 | 0.003970 | 0.012730 | 0.006077 | 0.018590 | 0.004226 |
Vacuole | 0.003403 | 0.002440 | 0.061159 | 0.017665 | 0.001901 | 0.002513 | 0.027903 | 0.013905 | 0.002459 | 0.002478 | 0.043694 | 0.014058 |
Vacuole Periphery | 0.004330 | 0.002470 | 0.015659 | 0.006203 | 0.002525 | 0.003210 | 0.009817 | 0.009684 | 0.003195 | 0.002855 | 0.012591 | 0.009542 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 6.37 | 0.41 | 5.60 | -0.23 | 1.47 | 1.44 | 0.55 | 0.65 | -0.80 | -0.02 | 6.16 | 0.10 | 5.31 | -0.71 | 1.46 |
Bud Neck | 2.74 | -2.58 | 1.40 | -2.58 | 3.23 | 2.30 | -1.97 | 2.36 | -0.03 | 3.33 | 3.59 | -3.28 | 3.39 | -1.20 | 4.71 |
Bud Site | 3.98 | -1.67 | 2.97 | -2.60 | 3.51 | 1.75 | -3.39 | -1.27 | -2.68 | 2.88 | 4.32 | -3.09 | 2.53 | -3.17 | 4.37 |
Cell Periphery | 6.45 | 3.44 | 5.78 | -2.08 | 3.09 | 3.15 | 2.13 | 3.71 | 0.70 | 3.33 | 7.14 | 3.47 | 7.24 | 0.09 | 4.72 |
Cytoplasm | 3.52 | 0.79 | 3.14 | -1.67 | 3.49 | -0.11 | -0.93 | -1.69 | -1.08 | -1.15 | 2.39 | -0.08 | -0.03 | -1.82 | 0.03 |
Cytoplasmic Foci | -18.91 | -8.97 | -9.36 | -3.29 | -1.11 | -12.13 | -9.72 | -4.04 | 9.06 | 8.65 | -21.57 | -13.03 | -8.04 | 12.75 | 10.22 |
Eisosomes | 13.21 | 9.86 | 12.92 | 0.98 | 1.97 | 7.64 | 5.67 | 9.21 | 2.14 | 1.70 | 14.77 | 10.23 | 15.24 | 2.25 | 2.53 |
Endoplasmic Reticulum | 2.69 | -1.19 | 0.95 | -1.10 | 1.87 | 3.83 | -1.98 | 2.58 | -1.44 | 3.74 | 3.34 | -1.79 | 3.06 | -0.94 | 3.45 |
Endosome | 4.99 | 1.08 | -4.97 | -7.04 | -4.84 | 4.07 | -2.11 | -17.03 | -22.22 | -4.11 | 6.66 | -0.86 | -19.84 | -27.76 | -8.20 |
Golgi | -1.83 | 9.18 | 13.77 | 15.64 | 1.30 | -0.84 | 13.69 | 14.23 | 15.50 | -8.22 | -1.63 | 16.23 | 16.28 | 19.31 | -9.01 |
Lipid Particles | 4.49 | 2.54 | 1.41 | -1.00 | -0.36 | 8.91 | 0.49 | 10.10 | 1.84 | 2.86 | 9.95 | 1.63 | 11.20 | 2.20 | 3.21 |
Mitochondria | 9.07 | 11.45 | 8.91 | 1.34 | -1.60 | 2.32 | 7.93 | 1.24 | -1.46 | -9.23 | 8.57 | 13.92 | 6.85 | -2.43 | -11.00 |
Mitotic Spindle | -3.75 | -3.58 | -2.17 | -1.52 | 3.15 | -1.67 | -2.52 | -4.87 | -3.66 | 2.00 | -3.72 | -4.35 | -5.12 | -3.58 | 3.80 |
None | 1.88 | 1.89 | 1.89 | 2.27 | 1.23 | 1.71 | 1.79 | 1.78 | 0.97 | -1.05 | 2.48 | 2.54 | 2.53 | 0.93 | -1.69 |
Nuclear Periphery | 1.51 | -2.10 | -0.88 | -1.03 | -0.04 | 0.64 | -3.11 | -6.48 | -6.55 | 1.98 | 1.51 | -2.66 | -0.80 | -2.39 | 2.41 |
Nuclear Periphery Foci | 0.70 | -2.55 | -1.29 | -1.40 | 1.66 | 2.28 | -1.83 | 1.51 | -1.57 | 2.30 | 1.98 | -2.80 | 0.62 | -1.69 | 2.86 |
Nucleolus | 1.77 | 1.34 | 1.78 | 0.23 | 1.47 | 2.14 | 1.78 | 2.15 | 1.21 | 1.78 | 2.75 | 2.15 | 2.81 | 1.16 | 2.22 |
Nucleus | 2.06 | 1.85 | 1.92 | -0.85 | 0.47 | 1.29 | 1.10 | 1.32 | 0.40 | 1.45 | 2.42 | 2.12 | 2.43 | 0.12 | 2.18 |
Peroxisomes | 4.16 | 0.83 | 1.31 | -1.43 | 0.40 | 4.19 | -2.20 | 6.63 | 1.98 | 3.65 | 5.82 | -1.70 | 8.39 | 2.20 | 4.37 |
Vacuole | 1.64 | -5.73 | -4.72 | -5.35 | 4.20 | -2.26 | -3.84 | -15.63 | -14.59 | 2.04 | 0.34 | -6.85 | -14.51 | -15.48 | 4.88 |
Vacuole Periphery | 3.36 | -3.47 | -1.30 | -2.60 | 2.68 | -1.31 | -2.64 | -11.82 | -9.28 | 0.05 | 0.90 | -4.42 | -11.18 | -11.20 | 1.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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