Standard name
Human Ortholog
Description RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0.07 0.11 0.18 0.19 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.13 0 0.11 0 0.08 0 – 0.07 0 0 0 0.2 0.17 0.09 0.07 0.15 0.06 0 0 0 0 0 0
Nucleus 0.09 0.16 0 0.07 0.06 0.05 0.08 – 0 0 0 0.05 0.08 0 0.06 0.07 0 0.08 0 0.09 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.95 0.97 0.97 0.88 0.86 0.89 0.83 – 0.86 0.86 0.81 0.84 0.92 0.85 0.79 0.75 0.79 0.7 0.84 0.82 0.82 0.77 0.71 0.61
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.11 0 0.22 0.3 0.34 – 0.31 0.46 0.38 0.46 0 0.05 0.06 0 0 0 0.07 0.05 0.12 0.08 0.16 0.19
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 2 – 2 1 1 0 0 0 0 0 0 0 2 0 1 1 2 3
Bud Neck 1 0 4 2 0 4 6 – 7 16 8 11 0 0 0 0 0 0 0 0 1 1 0 4
Bud Site 0 0 0 0 0 2 8 – 5 6 5 20 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 0 2 5 12 8 20 – 12 13 14 5 7 20 31 12 15 14 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 3 0 0 0 1 0 0
Endosome 2 0 0 3 22 4 20 – 9 10 8 3 0 11 6 0 1 1 1 1 1 1 1 1
Golgi 0 0 0 0 2 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0
Mitochondria 37 21 6 23 5 25 17 – 23 10 9 12 35 45 24 5 12 4 1 1 1 2 3 4
Nucleus 18 26 9 15 18 17 32 – 13 16 13 19 14 12 17 5 0 5 7 14 5 5 4 8
Nuclear Periphery 2 0 0 1 1 1 0 – 3 0 0 1 0 0 0 0 0 0 0 0 1 1 0 2
Nucleolus 197 155 226 191 243 281 327 – 295 353 254 311 162 226 217 50 63 46 181 131 198 102 145 121
Peroxisomes 0 0 1 1 3 10 11 – 12 3 3 12 1 7 9 1 0 1 0 0 0 0 1 2
SpindlePole 3 3 26 8 63 93 132 – 108 190 118 171 4 13 16 0 2 2 14 8 27 11 32 37
Vac/Vac Membrane 1 0 1 3 1 1 3 – 1 2 0 1 1 0 1 1 2 1 1 1 1 1 8 5
Unique Cell Count 208 159 233 216 283 314 392 344 411 314 370 177 267 273 67 80 66 217 159 243 132 205 198
Labelled Cell Count 262 206 275 252 370 447 578 490 620 433 566 224 335 321 74 97 77 217 159 243 132 205 198


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.5 23.0 18.6 16.7 16.4 9.3 10.3 – 10.4 9.3 9.2 8.9 23.0 21.3 20.2 28.8 35.6 32.4 19.9 23.0 22.7
Std Deviation (1e-4) 8.0 6.6 5.9 5.1 5.0 2.7 3.4 – 3.3 2.5 2.4 2.3 7.5 8.1 8.0 19.7 20.1 21.7 7.4 6.5 7.2
Intensity Change (Log2) – – – -0.15 -0.18 -0.99 -0.86 – -0.84 -1.0 -1.02 -1.07 0.3 0.2 0.12 0.63 0.94 0.8 0.1 0.31 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 1.5 0.7 1.0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 3.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.4 0 2.8 – 2.0 1.9 2.5 0 0 3.6 4.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.3 0 3.5 – 0 0 0 0 0 3.1 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.5 0 2.7 1.1 – 2.2 -0.1 0.2 0.5 5.7 5.3 3.0 0 0 0
Nucleus 1.5 1.3 0.8 2.1 – -0.1 0 0.2 0.7 1.8 0.4 1.2 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.5 -4.4 -3.3 -5.1 – -4.5 -4.5 -5.7 -5.0 -2.4 -4.7 -5.9 -5.9 -5.3 -6.8
Peroxisomes 0 0 0 0 – 2.4 0 0 2.3 0 0 0 0 0 0
SpindlePole -3.0 3.3 5.2 6.3 – 5.6 9.1 6.9 8.9 -3.4 -2.6 -2.2 -2.9 -2.3 -2.0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.9966 14.0705 13.7157 11.1381 13.2812 12.9362 16.0952 21.348 19.6909 17.0726 15.4999 19.8129 20.5688 21.6306 19.9947 18.767 18.7729 19.149
Actin 0 0 0 0 0 0.0001 0.0166 0.0001 0.0008 0.003 0.0044 0 0.0074 0 0 0 0.0001 0.0012
Bud 0 0 0 0 0 0 0.0003 0 0.0001 0.0003 0.0001 0 0 0 0 0 0 0
Bud Neck 0 0.0001 0.0001 0.0002 0.0003 0.0001 0.0003 0.0002 0.0001 0.0002 0.0002 0 0.0001 0 0 0 0.0001 0.0001
Bud Periphery 0 0 0 0 0.0001 0 0.0005 0 0.0003 0.0005 0.0002 0 0.0001 0 0 0 0 0
Bud Site 0.0001 0 0 0 0.0001 0 0.0011 0.0001 0.0004 0.001 0.0003 0 0.0001 0 0 0 0.0001 0
Cell Periphery 0.0001 0 0 0.0001 0.0002 0 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0001 0 0 0 0 0.0001 0.0052 0 0 0.0003 0 0 0.0001 0 0 0 0.0001 0.0001
Cytoplasmic Foci 0.0048 0.0001 0.0014 0.0034 0.0006 0.0006 0.0369 0.0007 0.0008 0.0067 0.0049 0.0001 0.0037 0.0001 0.0005 0.0002 0.008 0.0008
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0.0001 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.0004 0.0024 0 0.0002 0.0002 0.0001 0 0.0002 0 0 0 0 0.0001
Endosome 0 0 0.0001 0.0002 0 0.002 0.0204 0.0005 0.0021 0.0008 0.0034 0 0.0012 0 0 0 0.0002 0.001
Golgi 0.0001 0 0.0002 0.0005 0.0001 0.0001 0.0087 0.0011 0.0019 0.0004 0.01 0 0.0004 0 0 0 0.0002 0.0003
Lipid Particles 0.0042 0.0006 0.002 0.0078 0.0058 0.0015 0.0386 0.0021 0.0052 0.0058 0.0752 0.0001 0.0019 0.0001 0.0004 0.0003 0.0064 0.0028
Mitochondria 0.0015 0.0001 0.0001 0.0001 0.0052 0.0002 0.0075 0.0044 0.0047 0.0165 0.0348 0.0001 0.0014 0 0.0004 0.0001 0.0103 0.0003
None 0.0002 0 0 0 0 0.0001 0.0172 0 0.0001 0.0008 0.0001 0 0.0007 0 0.0001 0 0.0004 0.0003
Nuclear Periphery 0.0001 0.0038 0 0 0.0002 0.0011 0.0137 0 0.0008 0.003 0.0012 0 0.0013 0 0.0006 0 0.0007 0.0012
Nucleolus 0.9348 0.889 0.9306 0.878 0.9402 0.9386 0.7089 0.8987 0.9023 0.8885 0.8121 0.9398 0.9242 0.9122 0.9448 0.9553 0.8861 0.9353
Nucleus 0.0518 0.1028 0.0619 0.0671 0.0442 0.0505 0.0544 0.091 0.0774 0.0672 0.0374 0.0594 0.0502 0.0863 0.0503 0.0405 0.0677 0.0498
Peroxisomes 0.0002 0 0.0031 0.0359 0.0004 0 0.0058 0.0001 0.0002 0.0006 0.0056 0 0.0006 0 0.0001 0 0.0007 0.0004
Punctate Nuclear 0.0018 0.0029 0.0003 0.0065 0.0018 0.0041 0.0575 0.0003 0.0002 0.0024 0.0075 0.0003 0.0058 0.0011 0.0025 0.0034 0.0178 0.0062
Vacuole 0.0001 0.0001 0 0.0001 0.0002 0.0002 0.0015 0.0002 0.0012 0.0006 0.0007 0.0001 0.0001 0 0.0001 0 0.0002 0
Vacuole Periphery 0.0002 0.0003 0 0 0.0004 0.0001 0.0023 0.0003 0.001 0.0012 0.0015 0 0.0002 0 0.0001 0 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.7311 66.0394 71.9422 69.6884 86.6111 29.8477 56.4724 34.9987 48.0501 59.1639
Translational Efficiency 3.8124 3.9829 3.2184 3.0899 2.7038 5.1675 2.3522 4.0068 2.0395 2.8274

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2077 1248 193 599 1467 2018 225 1902 3544 3266 418 2501

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1523.46 1733.64 2153.95 2446.69 1487.50 1762.89 2275.62 2409.27 1508.57 1751.71 2219.44 2418.23
Standard Deviation 359.24 500.78 400.83 626.53 330.54 412.41 477.66 607.60 348.10 448.46 447.97 612.40
Intensity Change Log 2 — 0.186453 0.499633 0.683480 — 0.245053 0.613370 0.695706 — 0.215700 0.556943 0.689533

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000234 0.000306 0.000258 0.000410 0.000188 0.000312 0.000302 0.000477 0.000215 0.000310 0.000282 0.000461
Bud Neck 0.000990 0.001353 0.002209 0.003488 0.000775 0.001779 0.002232 0.003865 0.000901 0.001616 0.002222 0.003775
Bud Site 0.000256 0.000765 0.000807 0.001002 0.000261 0.000687 0.000813 0.001112 0.000258 0.000717 0.000810 0.001086
Cell Periphery 0.000011 0.000160 0.000012 0.000040 0.000012 0.000052 0.000015 0.000081 0.000012 0.000093 0.000014 0.000071
Cytoplasm 0.000062 0.001063 0.000028 0.000230 0.000105 0.000425 0.000064 0.001098 0.000080 0.000669 0.000048 0.000890
Cytoplasmic Foci 0.004758 0.008332 0.010222 0.011332 0.007152 0.005714 0.009176 0.007844 0.005749 0.006714 0.009659 0.008680
Eisosomes 0.000434 0.000352 0.000222 0.000276 0.000387 0.000419 0.000244 0.000341 0.000415 0.000393 0.000234 0.000325
Endoplasmic Reticulum 0.000095 0.000186 0.000075 0.000088 0.000116 0.000173 0.000074 0.000115 0.000104 0.000178 0.000074 0.000108
Endosome 0.000023 0.000172 0.000016 0.000232 0.000071 0.000053 0.000015 0.000243 0.000043 0.000099 0.000016 0.000240
Golgi 0.000123 0.000278 0.000449 0.002155 0.000205 0.000139 0.000298 0.000505 0.000157 0.000192 0.000368 0.000900
Lipid Particles 0.000023 0.000099 0.000018 0.000031 0.000178 0.000019 0.000014 0.000077 0.000087 0.000049 0.000016 0.000066
Mitochondria 0.000913 0.001190 0.001161 0.002009 0.000697 0.001077 0.001421 0.002710 0.000824 0.001120 0.001301 0.002542
Mitotic Spindle 0.005290 0.003502 0.027163 0.022847 0.005569 0.002872 0.010324 0.014686 0.005406 0.003113 0.018099 0.016641
None 0.000288 0.001402 0.000247 0.000655 0.000301 0.000919 0.000351 0.000732 0.000293 0.001104 0.000303 0.000713
Nuclear Periphery 0.000070 0.000053 0.000077 0.000244 0.000061 0.000079 0.000071 0.000289 0.000066 0.000069 0.000073 0.000278
Nuclear Periphery Foci 0.000132 0.000281 0.000068 0.000178 0.000376 0.000130 0.000048 0.000274 0.000233 0.000187 0.000057 0.000251
Nucleolus 0.635496* 0.571329* 0.579765* 0.614751* 0.583497* 0.569937* 0.614185* 0.668547* 0.613971* 0.570469* 0.598292* 0.655663*
Nucleus 0.156410* 0.192748* 0.086049 0.167539* 0.124809 0.269650* 0.100595 0.193804* 0.143329* 0.240264* 0.093879 0.187514*
Peroxisomes 0.193751* 0.214783* 0.290130* 0.169840* 0.274585* 0.144682* 0.258889* 0.100325* 0.227211* 0.171469* 0.273314* 0.116974*
Vacuole 0.000299 0.001039 0.000505 0.001333 0.000355 0.000442 0.000367 0.001119 0.000322 0.000670 0.000430 0.001170
Vacuole Periphery 0.000340 0.000606 0.000519 0.001321 0.000299 0.000442 0.000506 0.001755 0.000323 0.000505 0.000512 0.001651

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.67 -1.65 -7.13 -3.30 -4.28 -7.90 -3.23 -12.22 -6.79 -4.09 -7.97 -3.40 -13.50 -7.50 -6.15
Bud Neck -1.95 -4.57 -6.49 -5.46 -2.70 -5.34 -6.95 -11.82 -7.00 -5.15 -4.95 -7.66 -12.79 -9.04 -5.78
Bud Site -3.27 -7.55 -8.41 -1.78 -1.75 -2.36 -7.40 -9.84 -2.70 -3.12 -3.70 -10.54 -12.04 -3.26 -3.52
Cell Periphery -1.18 -1.13 -3.19 0.93 -2.99 -2.45 -0.52 -5.47 -1.57 -5.33 -1.69 -1.13 -6.18 0.35 -5.95
Cytoplasm -1.64 1.39 -3.13 1.33 -3.90 -1.93 0.82 -2.10 -1.50 -2.25 -2.30 1.10 -2.30 -0.64 -2.41
Cytoplasmic Foci -3.02 -3.81 -5.09 -2.16 -0.80 1.20 -1.37 -1.12 -2.73 0.39 -1.33 -4.05 -4.27 -2.96 0.30
Eisosomes 3.45 10.61 6.15 3.06 -2.14 -1.20 5.86 1.79 3.02 -3.59 1.20 11.37 4.47 3.47 -4.52
Endoplasmic Reticulum -2.28 1.65 0.55 2.37 -0.67 -1.00 1.94 0.24 1.17 -2.70 -2.24 2.34 -0.53 2.11 -3.32
Endosome -1.69 0.55 -1.19 -0.26 -1.23 0.41 1.30 -1.68 -2.06 -2.54 -1.38 1.42 -2.38 -1.56 -2.81
Golgi -2.45 -10.84 -1.23 -1.11 -1.02 2.19 -2.22 -2.45 -3.22 -1.78 -1.36 -9.40 -1.86 -1.74 -1.30
Lipid Particles -1.52 0.33 -0.66 1.43 -1.36 1.23 1.27 0.78 -1.83 -2.02 0.07 1.31 0.38 0.37 -2.11
Mitochondria -2.24 -2.56 -5.54 -3.77 -4.05 -4.41 -3.84 -4.85 -3.88 -2.84 -4.05 -4.28 -5.38 -4.40 -3.69
Mitotic Spindle 1.32 -2.61 -5.08 -5.48 0.14 2.94 -1.72 -5.74 -8.26 -1.49 3.05 -3.08 -7.84 -9.68 0.01
None -3.49 1.35 -5.36 2.24 -5.70 -3.08 -1.00 -4.87 0.59 -4.19 -4.67 -0.52 -6.08 1.82 -5.73
Nuclear Periphery 1.48 -0.68 -4.60 -5.00 -4.39 -1.22 -0.82 -7.15 -6.49 -6.76 -0.36 -0.96 -8.25 -8.00 -7.89
Nuclear Periphery Foci -1.36 2.43 -0.95 1.15 -2.13 1.78 2.15 1.16 -1.37 -2.46 0.19 2.52 0.51 0.25 -2.75
Nucleolus 8.64 4.40 2.56 -3.25 -2.09 1.81 -1.98 -10.67 -12.54 -3.87 8.45 2.10 -6.22 -12.94 -5.48
Nucleus -5.21 7.00 -1.16 2.32 -6.22 -21.83 2.14 -10.98 10.14 -8.16 -19.73 6.45 -8.60 8.68 -11.33
Peroxisomes -3.20 -7.62 2.99 5.16 8.65 21.04* 1.09 29.56* 10.05 13.78 12.97 -5.42 26.69 13.41 17.92
Vacuole -2.48 -1.59 -1.53 -0.34 -1.19 -0.51 -0.07 -3.45 -2.96 -3.65 -2.46 -1.47 -3.76 -1.93 -3.21
Vacuole Periphery -4.67 -2.10 -4.55 -3.19 -3.47 -2.41 -3.72 -5.04 -4.55 -4.35 -4.75 -3.95 -5.93 -5.07 -5.02
Standard name
Human Ortholog
Description RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p
Localization
Cell Percentages nucleolus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rpa34

Rpa34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpa34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available