Standard name
Human Ortholog
Description Gamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.18 0.13 0.16 0.09 0.08 0.08 0.08 0.08 0.07 0.08 0.12 0.16 0.16 0.09 0.18 0.11 0.12 0 0 0.08 0.06 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0.9 0.54 0.83 0.79 0.83 0.38 0.34 0.32 0.2 0.19 0.15 0.22 0.94 0.92 0.78 0.87 0.83 0.86 0.9 0.75 0.87 0.84 0.9 0.88
Mitochondria 0.3 0.61 0.65 0.64 0.52 0.8 0.79 0.74 0.88 0.89 0.91 0.85 0.29 0.24 0 0.05 0.07 0.08 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.07 0.13 0.05 0.09 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.1 0.05 0.05 0.05 0 0.05 0.05 0 0 0 0 0.18 0 0.13 0.11 0.13 0.09 0 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 34 22 46 51 40 34 49 51 36 43 36 73 8 4 4 66 47 45 5 3 28 3 8 8
Bud 0 3 4 3 2 3 7 15 6 5 5 10 1 0 1 7 5 4 1 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 3 1 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 3 0 0 1 2 3 2 1 5 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 9 10 9 10 13 16 3 5 5 4 5 6 0 1 5 14 36 21 1 2 0 0 0 0
Endoplasmic Reticulum 18 11 3 4 7 19 14 12 9 7 11 14 2 1 3 9 12 15 1 1 0 1 0 0
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2 1 6 6 3 0 1 1
Golgi 290 67 291 252 369 164 216 214 86 120 69 138 46 23 35 314 359 328 296 105 320 54 162 126
Mitochondria 97 75 229 206 232 343 503 495 384 570 418 524 14 6 2 19 31 32 3 2 6 3 2 3
Nucleus 0 0 0 0 0 0 1 2 0 1 0 1 0 0 0 0 0 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 0 0 2 1 2 2 7 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 9 2 7 7 13 13 45 85 20 60 39 50 1 0 1 5 13 11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 23 12 16 16 23 18 31 33 18 17 12 17 9 1 6 39 58 35 6 16 1 0 1 0
Unique Cell Count 321 123 352 320 443 431 634 668 437 642 460 614 49 25 45 360 433 380 328 141 367 65 180 143
Labelled Cell Count 481 204 606 553 699 611 870 917 572 832 601 846 81 36 58 474 563 495 328 141 367 65 180 143


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.2 25.8 31.7 30.1 30.8 26.4 26.4 25.2 25.3 24.4 23.1 24.3 29.5 28.0 29.8 22.2 25.3 31.8 34.1 35.8 36.6
Std Deviation (1e-4) 7.7 7.4 6.4 6.4 6.2 5.9 5.5 6.4 5.8 5.4 5.4 5.7 7.2 7.0 7.8 6.9 8.5 10.2 7.0 6.7 8.0
Intensity Change (Log2) – – – -0.07 -0.04 -0.26 -0.26 -0.33 -0.32 -0.37 -0.45 -0.38 -0.1 -0.18 -0.09 -0.51 -0.32 0.0 0.11 0.18 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.1 -1.8 -2.4 -2.7 -2.8 -2.2 -3.4 -2.5 -0.5 0.6 0 -0.8 1.9 -1.0 -0.5
Bud 0 0 0 0 1.2 0 0 0 0.6 0 0 0 0.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.3 0.9 0 0 -1.5 0 -1.6 -1.9 0 0 0 1.0 3.5 2.0
Endoplasmic Reticulum 0 0 3.0 1.6 1.2 1.4 0 1.7 1.6 0 0 0 1.7 2.0 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.3 0.2 -12.6 -14.6 -15.4 -17.6 -19.6 -19.2 -18.1 2.0 1.2 -0.8 1.7 0.1 1.4
Mitochondria -0.2 -3.6 4.6 4.9 3.0 7.7 9.0 9.1 7.3 -4.9 -4.1 -7.8 -16.7 -17.1 -16.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.8 0.9 3.4 5.7 2.0 4.4 4.0 3.9 0 0 0 -0.6 0.9 0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 0.4 -0.3 0.2 0.3 -0.3 -1.6 -1.5 -1.5 0 0 0 3.1 4.2 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.4298 37.9345 34.3576 31.6726 31.4003 35.7519 28.616 36.0625 34.6076 32.3563 32.4822 33.637 29.8997 37.5855 33.145 31.1475 36.0608 35.118
Actin 0.0521 0.0031 0.0191 0.0029 0.0083 0.0018 0.0979 0.0016 0.0024 0.012 0.0167 0.0036 0.0653 0.01 0.0072 0.0491 0.0001 0.0018
Bud 0.0005 0.0002 0.0018 0.0001 0.0002 0 0.0003 0 0.0001 0.002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0046 0 0
Bud Neck 0.0004 0.0002 0.0009 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0002 0.0003 0 0.0001
Bud Periphery 0.0006 0.0001 0.0019 0.0001 0.0002 0 0.0003 0 0.0001 0.002 0.0004 0.0003 0.0002 0 0.0002 0.0056 0 0
Bud Site 0.0008 0.0002 0.0034 0 0.0003 0 0.0004 0 0 0.0003 0.0003 0.0001 0.0002 0 0.0001 0.0014 0 0
Cell Periphery 0.0005 0.0003 0.0004 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0002 0 0.0001 0.0002 0 0.0001
Cytoplasm 0.0024 0.0035 0.006 0.0007 0.0029 0.0005 0.0372 0.0005 0.0024 0.0037 0.0002 0.0001 0.0004 0.0017 0.0005 0.0006 0 0.0001
Cytoplasmic Foci 0.0143 0.0128 0.0099 0.0084 0.0211 0.0045 0.0125 0.0097 0.0049 0.0076 0.0039 0.0019 0.0066 0.011 0.0065 0.0056 0.0024 0.0024
Eisosomes 0.0007 0.0001 0.0004 0 0.0002 0.0001 0.0008 0 0.0002 0.0001 0.0002 0.0003 0.0009 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0022 0.0006 0.0013 0.0008 0.0035 0.0004 0.0017 0.0003 0.0005 0.001 0.0004 0.0008 0.0004 0.0003 0.0005 0.0005 0.0001 0.0004
Endosome 0.0994 0.1186 0.0847 0.2035 0.0844 0.0441 0.0885 0.0726 0.0895 0.1534 0.0542 0.0305 0.0498 0.0967 0.086 0.0863 0.0138 0.0407
Golgi 0.6794 0.7719 0.7703 0.6179 0.7264 0.9015 0.6751 0.8947 0.7585 0.6789 0.8646 0.8206 0.66 0.852 0.8061 0.7283 0.983 0.8799
Lipid Particles 0.0143 0.0057 0.0084 0.0052 0.0578 0.0008 0.0017 0.0079 0.0032 0.0021 0.0019 0.0015 0.0014 0.0008 0.0025 0.0011 0 0.0008
Mitochondria 0.0762 0.0529 0.0411 0.0085 0.0384 0.0407 0.0758 0.0075 0.066 0.0686 0.0441 0.1214 0.169 0.0222 0.0588 0.0247 0.0002 0.0681
None 0.0036 0 0.0014 0 0.0075 0 0.0007 0 0 0.0001 0 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0049 0.0002 0.0026 0.0009 0.0013 0.0001 0.0008 0.0001 0.0007 0.0007 0.0002 0.0058 0.0001 0 0.0001 0.0003 0 0.0001
Nucleolus 0.0058 0.0001 0.0006 0 0.0002 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0
Nucleus 0.0031 0.0001 0.001 0.0001 0.0002 0 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0 0
Peroxisomes 0.0018 0.0003 0.0031 0.0001 0.0183 0.0001 0.0012 0.0001 0.0001 0.0002 0.0002 0.0002 0.0006 0.0001 0.0003 0.0003 0 0.0001
Punctate Nuclear 0.0074 0 0.0008 0 0.0012 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.0078 0.0112 0.0138 0.0275 0.0043 0.0006 0.0018 0.0008 0.0197 0.0249 0.0021 0.0012 0.0136 0.0007 0.0074 0.0345 0 0.0007
Vacuole Periphery 0.0219 0.018 0.0271 0.1228 0.0229 0.0048 0.0024 0.0039 0.0513 0.0415 0.0096 0.0111 0.0309 0.0038 0.0231 0.0563 0.0002 0.0047

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 173.0946 128.9174 106.61 151.6445 141.3799 140.5983 144.3776 129.0878 133.4517 160.904
Translational Efficiency 0.96 1.1951 1.2217 0.9131 0.939 1.1176 0.9723 0.8625 1.016 0.9472

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1514 336 1312 944 1480 1732 1811 1690 2994 2068 3123 2634

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2552.96 2416.66 3425.72 3440.11 1675.87 2269.21 3049.02 3392.82 2119.40 2293.17 3207.28 3409.77
Standard Deviation 513.65 534.53 541.66 675.05 486.42 418.72 573.22 661.91 665.34 442.97 590.23 667.04
Intensity Change Log 2 — -0.079156 0.424236 0.430284 — 0.437280 0.863435 1.017575 — 0.148058 0.614564 0.692246

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004173 0.010181 0.006065 0.004188 0.002609 0.004094 0.003374 0.002858 0.003400 0.005083 0.004505 0.003335
Bud Neck 0.001000 0.000955 0.001619 0.001388 0.000418 0.000405 0.000403 0.000507 0.000713 0.000494 0.000914 0.000823
Bud Site 0.003092 0.000588 0.000449 0.005960 0.001613 0.000880 0.000301 0.003296 0.002361 0.000832 0.000363 0.004251
Cell Periphery 0.000943 0.000513 0.000505 0.003166 0.000400 0.000243 0.000540 0.001098 0.000675 0.000287 0.000525 0.001839
Cytoplasm 0.000938 0.000066 0.000036 0.001234 0.001623 0.000331 0.000030 0.000223 0.001277 0.000288 0.000033 0.000585
Cytoplasmic Foci 0.011729 0.012028 0.005329 0.012466 0.019788 0.018039 0.004394 0.006139 0.015713 0.017063 0.004787 0.008407
Eisosomes 0.000145 0.000506 0.000466 0.000120 0.000130 0.000120 0.000260 0.000255 0.000137 0.000183 0.000346 0.000207
Endoplasmic Reticulum 0.002348 0.004710 0.003828 0.003252 0.003696 0.001911 0.004455 0.003419 0.003014 0.002366 0.004192 0.003359
Endosome 0.023673 0.013379 0.004210 0.019233 0.009583 0.013740 0.006033 0.010179 0.016708 0.013681 0.005267 0.013424
Golgi 0.749554* 0.860435* 0.776336* 0.699367* 0.795846* 0.850159* 0.856681* 0.867171* 0.772437* 0.851828* 0.822927* 0.807032*
Lipid Particles 0.029552 0.029742 0.079494 0.032003 0.010905 0.019633 0.046534 0.022513 0.020334 0.021275 0.060381 0.025914
Mitochondria 0.006106 0.018678 0.004909 0.003189 0.074787 0.030028 0.003709 0.004297 0.040057 0.028184 0.004213 0.003900
Mitotic Spindle 0.001642 0.000080 0.000268 0.005781 0.001091 0.000328 0.000054 0.000380 0.001370 0.000288 0.000144 0.002315
None 0.000272 0.000431 0.000569 0.000182 0.001188 0.000318 0.000312 0.000196 0.000724 0.000336 0.000420 0.000191
Nuclear Periphery 0.001452 0.000292 0.002440 0.001559 0.000650 0.000474 0.000608 0.000371 0.001056 0.000444 0.001377 0.000797
Nuclear Periphery Foci 0.004115 0.000469 0.001693 0.005877 0.001621 0.000679 0.000539 0.000674 0.002882 0.000645 0.001024 0.002538
Nucleolus 0.000708 0.000274 0.000115 0.000081 0.000739 0.000020 0.000023 0.000050 0.000724 0.000061 0.000062 0.000061
Nucleus 0.000472 0.000029 0.000037 0.000067 0.003335 0.000019 0.000015 0.000029 0.001887 0.000021 0.000025 0.000043
Peroxisomes 0.000275 0.000097 0.000029 0.000126 0.000177 0.000075 0.000018 0.000033 0.000227 0.000078 0.000023 0.000066
Vacuole 0.060235 0.013297 0.020078 0.119058 0.009816 0.010625 0.010665 0.021691 0.035312 0.011059 0.014620 0.056587
Vacuole Periphery 0.097575 0.033248 0.091526 0.081703 0.059984 0.047881 0.061053 0.054620 0.078993 0.045504 0.073855 0.064326

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.12 -1.48 -0.13 2.02 1.25 -1.57 -1.05 -0.46 1.18 0.59 -1.69 -1.53 -0.03 1.68 1.50
Bud Neck 0.48 -1.84 -1.32 -1.80 0.59 0.34 0.28 -0.76 -1.12 -0.95 2.26 -1.07 -0.67 -3.28 0.49
Bud Site 5.15 5.44 -2.29 -4.49 -4.60 1.29 2.70 -1.63 -2.50 -3.25 3.64 5.85 -2.40 -4.50 -5.38
Cell Periphery 2.70 3.36 -3.57 -4.29 -4.35 3.55 -2.27 -6.51 -8.45 -5.11 5.51 2.05 -4.89 -6.75 -5.70
Cytoplasm 2.95 3.04 -0.96 -3.04 -3.09 1.76 2.23 1.95 0.46 -3.84 2.46 3.30 1.40 -1.78 -3.72
Cytoplasmic Foci -0.10 6.59 -0.56 -0.39 -6.61 1.38 17.29 12.57 10.51 -2.65 -1.25 16.58 8.70 8.79 -6.24
Eisosomes -2.90 -2.81 0.62 3.09 3.02 0.35 -3.15 -1.71 -1.89 0.07 -1.53 -3.89 -1.41 -0.47 2.04
Endoplasmic Reticulum -2.18 -3.46 -1.65 1.27 1.01 5.20 -1.77 0.59 -3.91 2.22 2.16 -3.87 -1.00 -2.79 2.31
Endosome 5.44 14.13 2.53 -3.45 -13.50 -4.31 5.74 -0.85 3.63 -6.65 2.99 14.75 3.58 0.22 -14.41
Golgi -8.65 -2.85 4.31 11.16 6.68 -6.48 -7.50 -8.49 -2.38 -1.52 -12.02 -8.15 -4.83 6.94 2.77
Lipid Particles -0.03 -10.57 -0.62 -0.39 8.97 -4.10 -12.19 -5.18 -1.14 7.28 -0.42 -14.89 -2.60 -1.96 11.87
Mitochondria -3.46 1.03 1.97 4.20 1.30 8.36 15.08 14.84 9.54 -0.69 3.57 14.44 14.30 10.42 0.36
Mitotic Spindle 3.13 2.69 -2.51 -3.60 -3.47 1.82 2.55 1.59 -0.48 -2.52 3.26 3.76 -1.51 -3.52 -3.77
None -2.53 -6.40 1.93 4.02 8.43 8.01 8.09 9.09 4.53 4.56 6.66 5.28 9.10 5.81 9.64
Nuclear Periphery 5.60 -2.16 -0.42 -5.97 1.87 0.83 0.22 1.32 1.36 3.94 3.96 -1.40 1.52 -3.76 2.98
Nuclear Periphery Foci 4.99 3.19 -1.61 -6.13 -4.64 1.99 2.45 2.13 -0.00 -1.50 4.91 4.25 0.62 -5.29 -4.52
Nucleolus 0.98 1.51 1.59 0.75 0.50 2.58 2.57 2.48 -1.43 -1.24 2.77 2.79 2.79 -0.05 -0.03
Nucleus 1.51 1.48 1.35 -2.60 -2.23 2.92 2.92 2.91 -1.49 -2.01 3.20 3.19 3.16 -3.26 -2.81
Peroxisomes 2.30 3.24 1.84 -0.81 -3.06 2.70 4.19 3.80 3.31 -1.79 3.47 4.78 3.66 0.68 -3.48
Vacuole 12.12 13.08 -9.34 -16.82 -16.98 -0.60 -0.67 -6.98 -6.96 -7.02 14.22 12.32 -7.46 -18.07 -16.78
Vacuole Periphery 12.41 1.19 3.07 -9.17 1.94 3.60 -0.32 1.45 -2.16 1.90 11.61 1.66 4.74 -6.91 3.30
Standard name
Human Ortholog
Description Gamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
Localization
Cell Percentages nucleus (27%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec21

Sec21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available