Standard name | |
---|---|
Human Ortholog | |
Description | Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 19 | 100 | 43 | 16 | 21 | 31 | 19 | 16 | 19 | 20 | 12 | 54 | 48 | 52 | – | 10 | 15 | 16 | 23 | 68 | 30 | 48 | 27 |
Bud | 10 | 13 | 33 | 20 | 16 | 18 | 36 | 48 | 39 | 37 | 41 | 20 | 14 | 13 | 11 | – | 2 | 5 | 0 | 0 | 1 | 1 | 1 | 2 |
Bud Neck | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 5 | 10 | – | 1 | 0 | 9 | 10 | 7 | 10 | 16 | 19 |
Bud Site | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 33 | 37 | 120 | 84 | 45 | 68 | 92 | 38 | 66 | 47 | 62 | 64 | 166 | 185 | 202 | – | 23 | 45 | 77 | 68 | 214 | 111 | 198 | 194 |
Cytoplasm | 59 | 65 | 98 | 86 | 96 | 133 | 179 | 225 | 136 | 156 | 124 | 150 | 446 | 438 | 559 | – | 79 | 52 | 8 | 11 | 3 | 7 | 10 | 7 |
Endoplasmic Reticulum | 1 | 6 | 2 | 1 | 5 | 3 | 10 | 8 | 6 | 7 | 5 | 8 | 8 | 5 | 2 | – | 5 | 7 | 8 | 5 | 15 | 8 | 10 | 12 |
Endosome | 0 | 0 | 0 | 0 | 7 | 2 | 2 | 1 | 1 | 0 | 1 | 4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 |
Mitochondria | 2 | 1 | 26 | 23 | 21 | 91 | 138 | 238 | 171 | 224 | 216 | 172 | 2 | 0 | 0 | – | 10 | 6 | 5 | 4 | 8 | 15 | 16 | 17 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 2 | 1 | 0 | 0 | 2 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 51 | 26 | 35 | 24 | 21 | 20 | 14 | 11 | 0 | 23 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
SpindlePole | 1 | 0 | 12 | 7 | 4 | 4 | 6 | 13 | 7 | 5 | 4 | 9 | 15 | 11 | 6 | – | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 3 | 2 | 3 | 3 | 5 |
Unique Cell Count | 98 | 108 | 289 | 179 | 174 | 258 | 377 | 488 | 325 | 384 | 346 | 354 | 603 | 578 | 702 | 112 | 103 | 132 | 135 | 336 | 194 | 312 | 291 | |
Labelled Cell Count | 126 | 146 | 446 | 290 | 246 | 366 | 516 | 618 | 461 | 508 | 475 | 466 | 712 | 706 | 844 | 134 | 137 | 132 | 135 | 336 | 194 | 312 | 291 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.6 | 5.4 | 5.6 | 5.3 | 4.5 | 4.3 | 4.4 | 4.2 | 3.9 | 4.0 | 4.3 | 6.1 | 6.4 | 6.4 | 4.7 | 5.3 | 6.5 | 6.5 | 6.6 | 6.9 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.3 | 1.7 | 1.8 | 1.6 | 1.4 | 1.3 | 1.2 | 1.0 | 0.8 | 1.0 | 0.7 | 0.8 | 0.8 | 0.8 | 1.0 | 1.5 | 1.1 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.02 | -0.26 | -0.32 | -0.3 | -0.36 | -0.46 | -0.43 | -0.34 | 0.18 | 0.25 | 0.25 | -0.21 | -0.03 | 0.27 | 0.26 | 0.29 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.4 | -6.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -9.5 | -9.7 | -10.8 | – | -5.2 | -3.8 |
Bud | -0.1 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.7 | -5.7 | -6.8 | – | -3.1 | -1.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 1.1 | -3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.2 | -2.8 | -3.9 | – | -3.9 | 0.4 |
Cytoplasm | 3.0 | 4.5 | 4.2 | 3.5 | 3.3 | 2.0 | 1.8 | 0.5 | 2.2 | 11.5 | 12.0 | 13.8 | – | 6.6 | 3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 1.3 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.9 | -7.3 | -8.1 | – | 0 | -1.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | -0.9 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -10.6 | -10.4 | -11.4 | – | -4.8 | -4.6 |
SpindlePole | -0.1 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | -1.9 | -3.5 | – | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9802 | 2.3476 | 2.0001 | 1.7317 | 1.2589 | 2.4078 | 2.4262 | 3.7205 | 3.0466 | 2.4797 | 2.6873 | 3.0605 | 2.4652 | 3.2588 | 2.6512 | 2.8521 | 2.8026 | 2.6286 |
Actin | 0.063 | 0.023 | 0.0137 | 0.0082 | 0.0471 | 0.011 | 0.0365 | 0.0036 | 0.0304 | 0.0127 | 0.0402 | 0.0246 | 0.0294 | 0.0356 | 0.0192 | 0.0047 | 0.0104 | 0.025 |
Bud | 0.0033 | 0.0016 | 0.0022 | 0.0306 | 0.0025 | 0.0009 | 0.0009 | 0.0004 | 0.0011 | 0.0015 | 0.0009 | 0.0016 | 0.0009 | 0.0002 | 0.0005 | 0.0009 | 0.0006 | 0.0005 |
Bud Neck | 0.0041 | 0.0114 | 0.0116 | 0.0048 | 0.0285 | 0.0169 | 0.0067 | 0.0041 | 0.0162 | 0.0591 | 0.0176 | 0.0327 | 0.0092 | 0.0048 | 0.0114 | 0.0207 | 0.0196 | 0.0236 |
Bud Periphery | 0.0189 | 0.0051 | 0.0051 | 0.0155 | 0.0243 | 0.01 | 0.0061 | 0.0028 | 0.009 | 0.0115 | 0.0117 | 0.0148 | 0.0045 | 0.0013 | 0.0041 | 0.0094 | 0.0067 | 0.0076 |
Bud Site | 0.0081 | 0.1055 | 0.0427 | 0.007 | 0.0111 | 0.0021 | 0.0561 | 0.1056 | 0.0619 | 0.0265 | 0.005 | 0.0033 | 0.0274 | 0.0351 | 0.0391 | 0.0188 | 0.0101 | 0.0026 |
Cell Periphery | 0.0957 | 0.1616 | 0.1653 | 0.1088 | 0.258 | 0.2023 | 0.2189 | 0.3232 | 0.2925 | 0.2398 | 0.3605 | 0.2757 | 0.2096 | 0.2254 | 0.3082 | 0.2748 | 0.3106 | 0.2347 |
Cytoplasm | 0.314 | 0.5196 | 0.5558 | 0.5988 | 0.4186 | 0.5206 | 0.3941 | 0.4161 | 0.4307 | 0.5354 | 0.4842 | 0.5197 | 0.4525 | 0.5187 | 0.5052 | 0.4468 | 0.5173 | 0.5963 |
Cytoplasmic Foci | 0.0309 | 0.0089 | 0.0116 | 0.0044 | 0.028 | 0.0046 | 0.0216 | 0.0016 | 0.0038 | 0.0027 | 0.0035 | 0.0065 | 0.0132 | 0.0026 | 0.0027 | 0.0081 | 0.0059 | 0.0082 |
Eisosomes | 0.0096 | 0.0081 | 0.0103 | 0.0026 | 0.0071 | 0.0032 | 0.0117 | 0.0083 | 0.0074 | 0.0045 | 0.0066 | 0.0038 | 0.0174 | 0.0092 | 0.0147 | 0.0071 | 0.0035 | 0.0067 |
Endoplasmic Reticulum | 0.0168 | 0.0072 | 0.0097 | 0.0118 | 0.0071 | 0.0098 | 0.0077 | 0.0065 | 0.0057 | 0.0026 | 0.0023 | 0.0038 | 0.0143 | 0.0085 | 0.0045 | 0.0065 | 0.0074 | 0.0068 |
Endosome | 0.032 | 0.0012 | 0.0036 | 0.0007 | 0.0104 | 0.0137 | 0.017 | 0.0002 | 0.0019 | 0.0004 | 0.0003 | 0.0007 | 0.0025 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0012 |
Golgi | 0.01 | 0.0023 | 0.002 | 0.0025 | 0.0072 | 0.0028 | 0.0085 | 0.0005 | 0.0034 | 0.0013 | 0.0015 | 0.0012 | 0.0043 | 0.0009 | 0.0011 | 0.0007 | 0.0015 | 0.0012 |
Lipid Particles | 0.0177 | 0.0029 | 0.0091 | 0.0016 | 0.0092 | 0.0057 | 0.0223 | 0.0009 | 0.0017 | 0.0007 | 0.0007 | 0.0023 | 0.0066 | 0.0015 | 0.0016 | 0.003 | 0.0041 | 0.0046 |
Mitochondria | 0.0038 | 0.0014 | 0.0005 | 0.0022 | 0.003 | 0.0024 | 0.0039 | 0.0001 | 0.0092 | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 |
None | 0.2779 | 0.1359 | 0.1477 | 0.1927 | 0.1256 | 0.1664 | 0.1545 | 0.1235 | 0.1197 | 0.0954 | 0.0638 | 0.1045 | 0.1914 | 0.1544 | 0.0849 | 0.1923 | 0.0956 | 0.0703 |
Nuclear Periphery | 0.0363 | 0.0003 | 0.0004 | 0.0008 | 0.002 | 0.0125 | 0.0075 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0017 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0009 |
Nucleolus | 0.0009 | 0.0003 | 0.0005 | 0.0006 | 0.0011 | 0.0017 | 0.0009 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0004 |
Nucleus | 0.0082 | 0.0005 | 0.0007 | 0.0026 | 0.0015 | 0.0038 | 0.0061 | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.0003 | 0.0007 | 0.0006 | 0.0005 |
Peroxisomes | 0.0272 | 0.0006 | 0.0028 | 0.0003 | 0.0019 | 0.0005 | 0.0055 | 0.0001 | 0.0018 | 0.0002 | 0.0001 | 0.0004 | 0.0035 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0031 |
Punctate Nuclear | 0.0155 | 0.0004 | 0.0023 | 0.0011 | 0.0023 | 0.0052 | 0.006 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0009 | 0.0072 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.003 |
Vacuole | 0.0045 | 0.0021 | 0.0021 | 0.0024 | 0.003 | 0.0028 | 0.0044 | 0.0013 | 0.0017 | 0.0032 | 0.0005 | 0.002 | 0.002 | 0.0006 | 0.0014 | 0.0024 | 0.0042 | 0.0023 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.001 | 0.003 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.7049 | 9.6871 | 11.4166 | 11.8259 | 10.3308 | 5.7338 | 11.9552 | 11.4715 | 11.0627 | 14.2335 |
Translational Efficiency | 0.5783 | 0.6221 | 0.5989 | 0.4183 | 0.4391 | 1.1379 | 0.6651 | 0.4359 | 0.5009 | 0.3469 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
112 | 177 | 314 | 78 | 800 | 1365 | 2551 | 1496 | 912 | 1542 | 2865 | 1574 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1022.97 | 874.79 | 982.82 | 968.48 | 679.91 | 798.61 | 961.91 | 955.65 | 722.04 | 807.35 | 964.20 | 956.29 |
Standard Deviation | 198.97 | 117.75 | 104.56 | 151.55 | 79.68 | 95.27 | 109.17 | 128.62 | 152.02 | 101.08 | 108.87 | 129.88 |
Intensity Change Log 2 | — | -0.225755 | -0.057765 | -0.078970 | — | 0.232147 | 0.500558 | 0.491139 | — | -0.025194 | 0.191593 | 0.176230 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.029983 | 0.056740 | 0.066981 | 0.147034* | 0.022361 | 0.018767 | 0.044683 | 0.116794* | 0.023297 | 0.023126 | 0.047127 | 0.118293* |
Bud Neck | 0.018414 | 0.106070 | 0.023404 | 0.029437 | 0.068786 | 0.072259 | 0.052124 | 0.049033 | 0.062600 | 0.076140 | 0.048976 | 0.048062 |
Bud Site | 0.048776 | 0.083659 | 0.203577* | 0.214685* | 0.029500 | 0.029554 | 0.077742 | 0.130820* | 0.031867 | 0.035765 | 0.091533 | 0.134976* |
Cell Periphery | 0.029491 | 0.153375* | 0.067486 | 0.189447* | 0.096716 | 0.045925 | 0.146254* | 0.158479* | 0.088460 | 0.058259 | 0.137621* | 0.160014* |
Cytoplasm | 0.202635* | 0.313200* | 0.272515* | 0.139455* | 0.461612* | 0.521197* | 0.332457* | 0.247108* | 0.429808* | 0.497322* | 0.325888* | 0.241773* |
Cytoplasmic Foci | 0.075316 | 0.043984 | 0.061083 | 0.024224 | 0.063855 | 0.063871 | 0.012450 | 0.008306 | 0.065262 | 0.061588 | 0.017781 | 0.009095 |
Eisosomes | 0.017623 | 0.110913* | 0.024385 | 0.060130 | 0.096749 | 0.103710* | 0.115567* | 0.129155* | 0.087031 | 0.104536* | 0.105574* | 0.125735* |
Endoplasmic Reticulum | 0.007535 | 0.009044 | 0.059421 | 0.025971 | 0.006049 | 0.003352 | 0.015933 | 0.009328 | 0.006231 | 0.004006 | 0.020700 | 0.010153 |
Endosome | 0.036474 | 0.008465 | 0.005009 | 0.005688 | 0.008646 | 0.008093 | 0.000785 | 0.002215 | 0.012064 | 0.008136 | 0.001248 | 0.002387 |
Golgi | 0.025816 | 0.017090 | 0.003281 | 0.006392 | 0.029238 | 0.023843 | 0.001384 | 0.002629 | 0.028818 | 0.023068 | 0.001592 | 0.002815 |
Lipid Particles | 0.005076 | 0.010635 | 0.009044 | 0.010260 | 0.029310 | 0.018655 | 0.004140 | 0.003224 | 0.026333 | 0.017735 | 0.004678 | 0.003573 |
Mitochondria | 0.002509 | 0.002878 | 0.000283 | 0.000135 | 0.014802 | 0.008321 | 0.000775 | 0.002015 | 0.013293 | 0.007696 | 0.000721 | 0.001921 |
Mitotic Spindle | 0.000579 | 0.002800 | 0.000173 | 0.000499 | 0.000133 | 0.000362 | 0.000335 | 0.000966 | 0.000188 | 0.000641 | 0.000318 | 0.000943 |
None | 0.000055 | 0.000118 | 0.000219 | 0.000052 | 0.000785 | 0.001562 | 0.000562 | 0.000374 | 0.000695 | 0.001396 | 0.000524 | 0.000358 |
Nuclear Periphery | 0.000242 | 0.000138 | 0.000154 | 0.000093 | 0.000659 | 0.000105 | 0.000161 | 0.000080 | 0.000607 | 0.000109 | 0.000161 | 0.000081 |
Nuclear Periphery Foci | 0.001766 | 0.000771 | 0.006556 | 0.002520 | 0.000236 | 0.000351 | 0.000994 | 0.000789 | 0.000424 | 0.000399 | 0.001604 | 0.000874 |
Nucleolus | 0.000058 | 0.002500 | 0.000076 | 0.000018 | 0.000194 | 0.000439 | 0.000057 | 0.000034 | 0.000177 | 0.000675 | 0.000059 | 0.000033 |
Nucleus | 0.049591 | 0.019139 | 0.081870 | 0.018898 | 0.018156 | 0.033782 | 0.136005* | 0.044198 | 0.022016 | 0.032101 | 0.130072* | 0.042944 |
Peroxisomes | 0.002611 | 0.015755 | 0.001941 | 0.002398 | 0.037239 | 0.019325 | 0.001381 | 0.002210 | 0.032987 | 0.018915 | 0.001442 | 0.002219 |
Vacuole | 0.435675* | 0.038677 | 0.112424 | 0.122216 | 0.014194 | 0.025870 | 0.056159 | 0.091898 | 0.065955 | 0.027340 | 0.062325 | 0.093401 |
Vacuole Periphery | 0.009777 | 0.004050 | 0.000114 | 0.000448 | 0.000781 | 0.000657 | 0.000052 | 0.000345 | 0.001886 | 0.001046 | 0.000058 | 0.000350 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.41 | -3.59 | -5.19 | -3.92 | -3.67 | 1.38 | -7.93 | -19.34 | -21.11* | -15.06 | -0.06 | -8.60 | -19.77 | -20.56 | -15.23 |
Bud Neck | -7.20 | -1.46 | -1.94 | 5.88 | -1.09 | -0.72 | 4.15 | 4.83 | 6.45 | 1.37 | -3.01 | 3.74 | 3.90 | 7.88 | 0.56 |
Bud Site | -2.64 | -9.44 | -6.78 | -5.19 | -0.62 | -0.02 | -13.36 | -19.23 | -19.38 | -9.31 | -1.36 | -15.80 | -19.76 | -18.97 | -7.70 |
Cell Periphery | -6.68 | -3.82 | -5.45 | -1.02 | -4.18 | 7.38 | -6.54 | -7.18 | -19.63 | -1.63 | 4.57 | -7.21 | -9.04 | -16.90 | -3.43 |
Cytoplasm | -4.41 | -3.16 | 2.01 | 6.71 | 5.81 | -5.78 | 13.77 | 21.55 | 33.14 | 11.95 | -6.72 | 11.27 | 19.12 | 31.56 | 12.21 |
Cytoplasmic Foci | 2.36 | 1.00 | 4.25 | 2.25 | 4.98 | 0.01 | 13.36 | 14.44 | 19.24 | 3.81 | 0.75 | 12.96 | 15.48 | 19.42 | 7.25 |
Eisosomes | -7.93 | -1.42 | -4.39 | 3.61 | -3.89 | -1.15 | -3.29 | -4.79 | -3.89 | -2.34 | -3.17 | -3.61 | -6.30 | -3.35 | -3.88 |
Endoplasmic Reticulum | -0.75 | -9.99 | -3.05 | -2.76 | 4.29 | 3.27 | -8.65 | -2.93 | -6.43 | 5.22 | 2.79 | -12.12 | -3.59 | -6.49 | 7.81 |
Endosome | 2.69 | 3.12 | 2.94 | 0.90 | -0.23 | 0.53 | 10.85 | 7.66 | 7.10 | -3.23 | 2.40 | 8.05 | 6.82 | 7.32 | -2.54 |
Golgi | 0.63 | 2.30 | 1.85 | 2.53 | -1.20 | 2.36 | 15.47 | 14.02 | 13.85 | -1.90 | 2.34 | 14.18 | 12.93 | 14.14 | -1.93 |
Lipid Particles | -2.61 | -3.80 | -1.00 | 0.06 | -0.21 | 4.21 | 11.27 | 11.64 | 12.47 | 2.69 | 3.81 | 10.91 | 11.34 | 12.24 | 2.63 |
Mitochondria | -0.38 | 1.29 | 1.39 | 4.20 | 3.40 | 3.18 | 7.66 | 6.54 | 5.54 | -1.68 | 3.07 | 7.73 | 6.48 | 5.55 | -1.71 |
Mitotic Spindle | -1.16 | 1.03 | 0.43 | 1.24 | -0.36 | -1.75 | -1.13 | -2.15 | -1.50 | -1.44 | -1.74 | -0.83 | -2.00 | -0.52 | -1.48 |
None | -2.43 | -3.49 | 0.32 | 2.74 | 3.64 | -1.45 | 0.93 | 1.58 | 2.54 | 1.93 | -1.48 | 0.82 | 1.47 | 2.50 | 1.83 |
Nuclear Periphery | 1.92 | 1.92 | 3.15 | 0.94 | 2.89 | 1.07 | 0.96 | 1.11 | 2.48 | 10.72 | 1.11 | 0.99 | 1.17 | 2.62 | 11.22 |
Nuclear Periphery Foci | 4.44 | -7.41 | -1.35 | -3.13 | 4.92 | -2.11 | -13.72 | -8.88 | -5.75 | 2.11 | 0.40 | -12.60 | -6.61 | -6.16 | 6.25 |
Nucleolus | -1.42 | -1.79 | 3.41 | 1.44 | 5.34 | -0.87 | 1.55 | 1.82 | 1.51 | 2.42 | -1.61 | 1.51 | 1.86 | 2.08 | 3.08 |
Nucleus | 2.98 | -3.28 | 2.83 | 0.03 | 9.18 | -7.71 | -34.41 | -10.35 | -4.33 | 23.96 | -4.73 | -32.57 | -8.12 | -4.73 | 24.48 |
Peroxisomes | -4.78 | 0.94 | 0.00 | 4.79 | -0.99 | 6.34 | 13.86 | 13.43 | 14.47 | -2.24 | 5.55 | 13.69 | 13.23 | 15.17 | -2.21 |
Vacuole | 13.54 | 10.58 | 9.62 | -4.58 | 0.07 | -7.08 | -21.80 | -20.21 | -17.15 | -9.47 | 6.61 | 0.62 | -4.39 | -17.39 | -8.17 |
Vacuole Periphery | 0.76 | 1.58 | 1.52 | 1.29 | -0.92 | 1.01 | 8.95 | 2.36 | 1.67 | -1.68 | 0.94 | 2.52 | 2.04 | 1.92 | -1.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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