Standard name | |
---|---|
Human Ortholog | |
Description | Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 117 | 69 | 50 | 30 | 15 | 33 | 22 | 23 | 21 | 29 | 19 | 20 | – | – | – | 49 | 64 | 36 | 129 | 60 | 107 | 5 | 8 | 8 |
Bud | 52 | 55 | 29 | 21 | 32 | 48 | 35 | 30 | 34 | 48 | 31 | 35 | – | – | – | 24 | 17 | 17 | 83 | 76 | 43 | 9 | 9 | 11 |
Bud Neck | 2 | 1 | 0 | 3 | 1 | 3 | 1 | 1 | 2 | 0 | 1 | 1 | – | – | – | 2 | 6 | 1 | 47 | 55 | 33 | 2 | 5 | 2 |
Bud Site | 19 | 33 | 16 | 8 | 13 | 12 | 16 | 13 | 9 | 12 | 7 | 14 | – | – | – | 9 | 25 | 6 | – | – | – | – | – | – |
Cell Periphery | 25 | 8 | 24 | 10 | 4 | 11 | 16 | 13 | 19 | 29 | 31 | 27 | – | – | – | 18 | 9 | 11 | 5 | 2 | 6 | 0 | 0 | 0 |
Cytoplasm | 209 | 186 | 146 | 104 | 129 | 207 | 213 | 234 | 166 | 207 | 171 | 189 | – | – | – | 223 | 197 | 116 | 157 | 137 | 83 | 2 | 10 | 8 |
Endoplasmic Reticulum | 13 | 10 | 8 | 10 | 3 | 22 | 13 | 23 | 16 | 27 | 19 | 23 | – | – | – | 17 | 8 | 11 | 16 | 7 | 22 | 0 | 3 | 0 |
Endosome | 20 | 13 | 26 | 12 | 32 | 33 | 22 | 25 | 17 | 11 | 11 | 15 | – | – | – | 10 | 12 | 6 | 21 | 17 | 11 | 1 | 0 | 2 |
Golgi | 10 | 0 | 4 | 3 | 1 | 1 | 0 | 4 | 2 | 0 | 0 | 0 | – | – | – | 5 | 9 | 3 | 10 | 4 | 3 | 0 | 0 | 1 |
Mitochondria | 10 | 24 | 5 | 14 | 11 | 101 | 160 | 164 | 187 | 303 | 236 | 316 | – | – | – | 20 | 28 | 2 | 5 | 12 | 7 | 0 | 1 | 0 |
Nucleus | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | – | – | – | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 2 | 2 | 2 | 3 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 1 | 6 | 2 | 2 | 4 | 10 | 17 | 14 | 7 | 13 | 10 | 13 | – | – | – | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 18 | 15 | 39 | 19 | 32 | 28 | 24 | 17 | 10 | 13 | 9 | 15 | – | – | – | 12 | 19 | 6 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 13 | 7 | 13 | 7 | 15 | 7 | 10 | 11 | 7 | 7 | 7 | 15 | – | – | – | 25 | 32 | 17 | 6 | 10 | 5 | 1 | 0 | 1 |
Vac/Vac Membrane | 9 | 7 | 6 | 1 | 12 | 21 | 13 | 38 | 36 | 36 | 24 | 28 | – | – | – | 14 | 8 | 11 | 1 | 12 | 1 | 0 | 1 | 0 |
Unique Cell Count | 396 | 340 | 275 | 187 | 244 | 411 | 440 | 497 | 410 | 559 | 432 | 539 | 333 | 327 | 199 | 492 | 405 | 332 | 25 | 43 | 40 | |||
Labelled Cell Count | 520 | 434 | 369 | 244 | 304 | 539 | 566 | 616 | 535 | 738 | 578 | 715 | 429 | 437 | 245 | 492 | 405 | 332 | 25 | 43 | 40 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 7.9 | 8.2 | 8.0 | 7.6 | 6.8 | 6.4 | 6.7 | 6.5 | 5.9 | 6.1 | 5.9 | – | – | – | 7.0 | 7.7 | 9.8 | – | – | – |
Std Deviation (1e-4) | 1.2 | 1.1 | 1.7 | 1.5 | 1.1 | 1.3 | 1.3 | 1.3 | 1.3 | 1.2 | 1.7 | 1.2 | – | – | – | 1.2 | 1.4 | 1.7 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.03 | -0.12 | -0.27 | -0.35 | -0.29 | -0.33 | -0.49 | -0.44 | -0.47 | – | – | – | -0.23 | -0.1 | 0.25 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.6 | -4.1 | -4.0 | -5.7 | -6.2 | -5.5 | -6.0 | -6.0 | -7.0 | – | – | – | -1.2 | 0.4 | 0 |
Bud | 0.2 | 0.9 | 0.5 | -1.2 | -2.3 | -1.0 | -0.9 | -1.6 | -2.0 | – | – | – | -1.5 | -2.5 | -0.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | -0.7 | -0.2 | -1.9 | -1.4 | -2.2 | -2.5 | -2.8 | -3.1 | -2.3 | – | – | – | -1.9 | 0.9 | -1.4 |
Cell Periphery | -1.4 | -3.6 | -3.5 | -2.9 | -3.8 | -2.2 | -2.0 | -0.8 | -2.1 | – | – | – | -1.6 | -3.2 | -1.3 |
Cytoplasm | 0.5 | -0.1 | -0.7 | -1.2 | -1.6 | -3.2 | -4.4 | -3.5 | -4.9 | – | – | – | 3.5 | 1.8 | 1.1 |
Endoplasmic Reticulum | 1.3 | -1.3 | 1.5 | 0 | 1.2 | 0.7 | 1.3 | 1.0 | 1.0 | – | – | – | 1.4 | -0.4 | 1.4 |
Endosome | -1.2 | 1.3 | -0.7 | -2.3 | -2.4 | -2.8 | -4.9 | -4.0 | -4.1 | – | – | – | -3.4 | -2.9 | -2.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1.1 | 0 |
Mitochondria | 3.0 | 1.8 | 8.1 | 10.7 | 10.0 | 12.5 | 14.7 | 14.4 | 15.7 | – | – | – | 2.6 | 3.6 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.5 | 2.0 | 0 | 0 | 0 | 1.7 | – | – | – | 0 | 0 | 0 |
Peroxisomes | -1.3 | -0.4 | -3.2 | -4.0 | -5.5 | -5.8 | -6.7 | -6.2 | -6.2 | – | – | – | -4.7 | -3.5 | -4.1 |
SpindlePole | -0.5 | 0.7 | -2.3 | -1.8 | -1.9 | -2.3 | -3.1 | -2.4 | -1.4 | – | – | – | 1.4 | 2.4 | 1.7 |
Vacuole | 0 | 1.7 | 1.9 | 0.6 | 3.1 | 3.5 | 2.6 | 2.2 | 2.0 | – | – | – | 1.4 | 0.2 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9474 | 5.0397 | 4.5419 | 4.3737 | 4.1666 | 4.534 | 5.3884 | 6.4863 | 6.3069 | 5.0048 | 6.0436 | 6.5267 | 6.6377 | 7.0366 | 6.8366 | 6.6628 | 6.562 | 6.9565 |
Actin | 0.148 | 0.0822 | 0.1435 | 0.0887 | 0.1688 | 0.1276 | 0.1617 | 0.0407 | 0.155 | 0.1945 | 0.2781 | 0.1172 | 0.2191 | 0.0695 | 0.1575 | 0.195 | 0.2429 | 0.1676 |
Bud | 0.043 | 0.0208 | 0.1364 | 0.1055 | 0.0415 | 0.006 | 0.0411 | 0.0283 | 0.1351 | 0.0654 | 0.0478 | 0.0077 | 0.0323 | 0.0475 | 0.1413 | 0.0779 | 0.0384 | 0.0075 |
Bud Neck | 0.0356 | 0.0078 | 0.0072 | 0.0041 | 0.0044 | 0.4356 | 0.0547 | 0.0092 | 0.0152 | 0.0036 | 0.0728 | 0.4882 | 0.035 | 0.0054 | 0.012 | 0.0191 | 0.0724 | 0.493 |
Bud Periphery | 0.0542 | 0.021 | 0.1371 | 0.1438 | 0.0374 | 0.0042 | 0.0509 | 0.0162 | 0.1782 | 0.123 | 0.0703 | 0.008 | 0.0492 | 0.0343 | 0.2018 | 0.1358 | 0.0718 | 0.0127 |
Bud Site | 0.1399 | 0.35 | 0.0702 | 0.0136 | 0.0116 | 0.0097 | 0.1702 | 0.3382 | 0.0886 | 0.0732 | 0.0231 | 0.012 | 0.1417 | 0.3257 | 0.0978 | 0.0227 | 0.0282 | 0.0064 |
Cell Periphery | 0.0048 | 0.0034 | 0.0032 | 0.0019 | 0.0009 | 0.0008 | 0.0034 | 0.0032 | 0.0019 | 0.0048 | 0.0017 | 0.0009 | 0.0052 | 0.0013 | 0.0023 | 0.0018 | 0.0019 | 0.0018 |
Cytoplasm | 0.3429 | 0.4141 | 0.3425 | 0.4662 | 0.4462 | 0.2041 | 0.2948 | 0.4485 | 0.315 | 0.2878 | 0.3354 | 0.1881 | 0.381 | 0.4453 | 0.296 | 0.433 | 0.4079 | 0.1877 |
Cytoplasmic Foci | 0.0691 | 0.0416 | 0.0309 | 0.0454 | 0.1236 | 0.1166 | 0.0952 | 0.0453 | 0.0405 | 0.0701 | 0.0531 | 0.1073 | 0.0569 | 0.0238 | 0.0435 | 0.0459 | 0.0444 | 0.0704 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0299 | 0.0059 | 0.0057 | 0.0093 | 0.0076 | 0.0086 | 0.0069 | 0.0023 | 0.0025 | 0.0044 | 0.0121 | 0.0023 | 0.0131 | 0.0047 | 0.0023 | 0.003 | 0.0034 | 0.0036 |
Endosome | 0.0495 | 0.0182 | 0.0393 | 0.0465 | 0.0707 | 0.0297 | 0.0394 | 0.0133 | 0.0202 | 0.0231 | 0.0197 | 0.0148 | 0.0167 | 0.0191 | 0.0139 | 0.021 | 0.0413 | 0.0123 |
Golgi | 0.0271 | 0.0115 | 0.0232 | 0.0244 | 0.0408 | 0.0323 | 0.0257 | 0.0073 | 0.0125 | 0.0129 | 0.0322 | 0.0113 | 0.0191 | 0.0058 | 0.0131 | 0.0194 | 0.0205 | 0.0132 |
Lipid Particles | 0.0063 | 0.0013 | 0.0024 | 0.001 | 0.0149 | 0.0021 | 0.0078 | 0.0007 | 0.001 | 0.0065 | 0.0097 | 0.0024 | 0.0023 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0007 |
Mitochondria | 0.0034 | 0.0037 | 0.012 | 0.007 | 0.0077 | 0.0029 | 0.0108 | 0.0011 | 0.0032 | 0.0443 | 0.003 | 0.0068 | 0.0028 | 0.0011 | 0.0039 | 0.0042 | 0.0029 | 0.0014 |
None | 0.0239 | 0.0021 | 0.0204 | 0.0011 | 0.0025 | 0.003 | 0.0092 | 0.0135 | 0.0153 | 0.0157 | 0.0078 | 0.0087 | 0.0126 | 0.0034 | 0.003 | 0.0058 | 0.0144 | 0.003 |
Nuclear Periphery | 0.004 | 0.0008 | 0.0015 | 0.003 | 0.0008 | 0.0005 | 0.0013 | 0.0003 | 0.0005 | 0.0017 | 0.0009 | 0.0026 | 0.0008 | 0.0027 | 0.0006 | 0.0007 | 0.0006 | 0.0002 |
Nucleolus | 0.0017 | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0092 | 0.0004 | 0.0011 | 0.0098 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0027 | 0.0016 | 0.0015 | 0.0023 | 0.0008 | 0.0006 | 0.0013 | 0.0039 | 0.0008 | 0.002 | 0.0017 | 0.0011 | 0.0008 | 0.0014 | 0.001 | 0.0009 | 0.0006 | 0.0004 |
Peroxisomes | 0.0045 | 0.0077 | 0.0081 | 0.0131 | 0.0137 | 0.0098 | 0.016 | 0.0041 | 0.0086 | 0.051 | 0.0142 | 0.0175 | 0.0073 | 0.0027 | 0.0053 | 0.0072 | 0.0022 | 0.0109 |
Punctate Nuclear | 0.0039 | 0.0013 | 0.0027 | 0.0008 | 0.0017 | 0.0031 | 0.0036 | 0.0011 | 0.0016 | 0.0034 | 0.0039 | 0.0011 | 0.0008 | 0.0008 | 0.0009 | 0.0008 | 0.0005 | 0.0051 |
Vacuole | 0.0046 | 0.0037 | 0.0086 | 0.0183 | 0.0028 | 0.0017 | 0.0044 | 0.0133 | 0.0031 | 0.0092 | 0.002 | 0.001 | 0.0023 | 0.0039 | 0.0025 | 0.0039 | 0.0043 | 0.0012 |
Vacuole Periphery | 0.0007 | 0.0004 | 0.0025 | 0.0037 | 0.0011 | 0.0005 | 0.0006 | 0.0004 | 0.0007 | 0.0017 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0005 | 0.0011 | 0.001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.0863 | 19.9006 | 24.0768 | 30.6741 | 32.628 | 39.1782 | 28.7605 | 26.866 | 31.2839 | 39.7773 |
Translational Efficiency | 0.7898 | 0.7387 | 0.6787 | 0.6884 | 0.5889 | 0.6626 | 0.5248 | 0.6201 | 0.5956 | 0.5566 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2399 | 1440 | 644 | 1724 | 1318 | 98 | 2921 | 2171 | 3717 | 1538 | 3565 | 3895 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1004.74 | 925.40 | 1172.36 | 1097.62 | 884.59 | 981.39 | 1083.34 | 1113.45 | 962.14 | 928.97 | 1099.42 | 1106.44 |
Standard Deviation | 110.19 | 179.81 | 136.64 | 152.13 | 125.14 | 122.93 | 144.63 | 143.79 | 129.20 | 177.26 | 147.25 | 147.75 |
Intensity Change Log 2 | — | -0.118673 | 0.222593 | 0.127556 | — | 0.149818 | 0.292405 | 0.331956 | — | 0.013271 | 0.255700 | 0.226870 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.016551 | 0.021168 | 0.066610 | 0.022510 | 0.019724 | 0.019543 | 0.018394 | 0.017585 | 0.017676 | 0.021065 | 0.027104 | 0.019765 |
Bud Neck | 0.056245 | 0.043489 | 0.033183 | 0.025302 | 0.097431 | 0.058349 | 0.045119 | 0.034396 | 0.070849 | 0.044436 | 0.042963 | 0.030371 |
Bud Site | 0.427248* | 0.384889* | 0.474530* | 0.295841* | 0.423026* | 0.354530* | 0.432168* | 0.312805* | 0.425751* | 0.382954* | 0.439820* | 0.305296* |
Cell Periphery | 0.000306 | 0.000983 | 0.001063 | 0.000432 | 0.002630 | 0.002738 | 0.000302 | 0.000272 | 0.001130 | 0.001095 | 0.000439 | 0.000343 |
Cytoplasm | 0.418955* | 0.272657* | 0.223411* | 0.485764* | 0.268686* | 0.291614* | 0.437956* | 0.513879* | 0.365672* | 0.273865* | 0.399200* | 0.501435* |
Cytoplasmic Foci | 0.017229 | 0.151240 | 0.093230 | 0.057653 | 0.097712 | 0.137898 | 0.020863 | 0.038457 | 0.045767 | 0.150390 | 0.033935 | 0.046954 |
Eisosomes | 0.000078 | 0.000368 | 0.000204 | 0.000130 | 0.001102 | 0.000795 | 0.000074 | 0.000091 | 0.000441 | 0.000395 | 0.000097 | 0.000108 |
Endoplasmic Reticulum | 0.000125 | 0.001989 | 0.001343 | 0.000571 | 0.002176 | 0.001897 | 0.000266 | 0.000218 | 0.000853 | 0.001983 | 0.000461 | 0.000374 |
Endosome | 0.006080 | 0.032823 | 0.013759 | 0.005846 | 0.024960 | 0.040805 | 0.002379 | 0.004845 | 0.012775 | 0.033332 | 0.004435 | 0.005288 |
Golgi | 0.002757 | 0.028259 | 0.005339 | 0.002947 | 0.018660 | 0.021193 | 0.001337 | 0.003120 | 0.008396 | 0.027808 | 0.002060 | 0.003044 |
Lipid Particles | 0.000190 | 0.004919 | 0.004460 | 0.002148 | 0.001930 | 0.002129 | 0.000532 | 0.000725 | 0.000807 | 0.004741 | 0.001242 | 0.001355 |
Mitochondria | 0.001670 | 0.004474 | 0.000133 | 0.001856 | 0.007336 | 0.000945 | 0.000477 | 0.001319 | 0.003679 | 0.004249 | 0.000415 | 0.001557 |
Mitotic Spindle | 0.008250 | 0.004978 | 0.003834 | 0.011457 | 0.001141 | 0.001487 | 0.004403 | 0.009499 | 0.005730 | 0.004755 | 0.004300 | 0.010365 |
None | 0.000080 | 0.001698 | 0.000096 | 0.000156 | 0.000184 | 0.000227 | 0.000183 | 0.000094 | 0.000117 | 0.001605 | 0.000168 | 0.000122 |
Nuclear Periphery | 0.000018 | 0.000056 | 0.000044 | 0.000131 | 0.000038 | 0.000034 | 0.000030 | 0.000050 | 0.000025 | 0.000055 | 0.000032 | 0.000086 |
Nuclear Periphery Foci | 0.000048 | 0.000985 | 0.002461 | 0.000725 | 0.000336 | 0.000350 | 0.000107 | 0.000240 | 0.000150 | 0.000945 | 0.000532 | 0.000455 |
Nucleolus | 0.000041 | 0.000559 | 0.000036 | 0.000083 | 0.000089 | 0.000101 | 0.000041 | 0.000099 | 0.000058 | 0.000530 | 0.000040 | 0.000092 |
Nucleus | 0.001243 | 0.003580 | 0.003447 | 0.006192 | 0.001498 | 0.002483 | 0.005730 | 0.003474 | 0.001334 | 0.003510 | 0.005318 | 0.004677 |
Peroxisomes | 0.016195 | 0.020447 | 0.023517 | 0.012256 | 0.011182 | 0.015089 | 0.011047 | 0.013598 | 0.014418 | 0.020105 | 0.013299 | 0.013004 |
Vacuole | 0.025861 | 0.017566 | 0.048943 | 0.066457 | 0.017469 | 0.043387 | 0.018401 | 0.044312 | 0.022885 | 0.019211 | 0.023918 | 0.054114 |
Vacuole Periphery | 0.000828 | 0.002873 | 0.000355 | 0.001543 | 0.002691 | 0.004406 | 0.000194 | 0.000922 | 0.001489 | 0.002970 | 0.000223 | 0.001197 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.81 | -7.27 | -1.40 | 0.64 | 6.73 | 0.03 | 0.87 | 1.57 | 0.45 | 0.87 | -1.43 | -4.10 | 0.06 | 1.48 | 4.18 |
Bud Neck | 2.95 | 6.21 | 12.32 | 7.11 | 3.35 | 3.19 | 9.84 | 12.11 | 2.51 | 4.93 | 6.78 | 10.10 | 16.28 | 6.11 | 7.48 |
Bud Site | 2.85 | -2.32 | 18.63* | 12.82 | 13.81 | 2.00 | 1.73 | 13.42 | 2.29 | 14.63* | 3.59 | 0.69 | 22.61 | 12.57 | 21.02* |
Cell Periphery | -4.68 | -3.02 | -1.50 | 4.09 | 2.68 | -0.12 | 7.18 | 7.31 | 3.11 | 1.89 | 0.15 | 5.55 | 6.82 | 5.44 | 2.44 |
Cytoplasm | 15.27 | 14.59 | -7.32 | -21.21 | -19.52 | -0.85 | -18.98 | -25.41 | -8.31 | -8.11 | 10.79 | -5.51 | -19.81 | -26.97 | -13.44 |
Cytoplasmic Foci | -25.81 | -13.63 | -16.87 | 15.82 | 5.43 | -2.26 | 17.72 | 12.19 | 5.47 | -9.45 | -20.20 | 4.77 | -2.30 | 19.44 | -7.66 |
Eisosomes | -2.89 | -5.29 | -3.15 | 2.50 | 3.41 | 1.17 | 6.16 | 6.08 | 3.44 | -1.43 | 0.41 | 5.73 | 5.67 | 3.11 | -0.44 |
Endoplasmic Reticulum | -5.80 | -6.10 | -5.63 | 4.32 | 3.88 | 0.30 | 4.13 | 4.40 | 2.00 | 1.06 | -3.29 | 1.90 | 2.94 | 5.25 | 1.67 |
Endosome | -11.99 | -4.48 | 1.06 | 12.52 | 5.07 | -2.07 | 11.85 | 10.48 | 4.82 | -4.17 | -9.31 | 9.55 | 9.21 | 13.56 | -1.00 |
Golgi | -14.98 | -2.76 | 0.74 | 14.89 | 2.96 | -0.37 | 14.99 | 12.33 | 2.81 | -2.38 | -11.46 | 12.92 | 9.73 | 15.07 | -1.37 |
Lipid Particles | -4.91 | -5.06 | -6.44 | 2.27 | 2.13 | -0.32 | 4.78 | 3.97 | 1.98 | -1.65 | -4.35 | -2.68 | -4.38 | 3.37 | -1.14 |
Mitochondria | -4.70 | 5.73 | 0.40 | 4.38 | -3.01 | 4.75 | 5.25 | 4.66 | -0.48 | -2.49 | -0.84 | 6.50 | 4.35 | 5.26 | -3.48 |
Mitotic Spindle | 2.41 | 2.57 | -0.85 | -2.74 | -2.87 | -0.54 | -5.11 | -5.22 | -4.24 | -2.48 | 0.93 | 1.06 | -2.72 | -3.43 | -3.69 |
None | -4.27 | -0.99 | -7.03 | 3.99 | -3.24 | -0.32 | -0.47 | 2.20 | 0.76 | 4.88 | -4.19 | -3.16 | -1.22 | 4.13 | 2.69 |
Nuclear Periphery | -2.41 | -4.76 | -2.21 | -1.45 | -1.43 | 0.13 | -0.83 | -0.96 | -1.75 | -0.29 | -1.77 | -2.92 | -2.70 | -1.42 | -1.46 |
Nuclear Periphery Foci | -2.68 | -5.76 | -8.84 | 0.18 | 3.88 | -0.09 | 0.93 | 0.11 | 0.45 | -4.72 | -2.39 | -4.18 | -5.07 | 1.12 | 0.50 |
Nucleolus | -2.30 | 0.45 | -4.84 | 2.11 | -6.19 | -0.28 | 1.56 | -0.33 | 0.08 | -1.53 | -2.25 | 1.05 | -1.78 | 2.07 | -2.50 |
Nucleus | -2.96 | -5.55 | -15.50 | -7.44 | -7.50 | -1.57 | -8.99 | -10.65 | -3.70 | 5.43 | -3.11 | -9.51 | -18.49 | -5.21 | 2.69 |
Peroxisomes | -2.28 | -2.51 | 3.62 | 5.19 | 4.72 | -0.68 | 0.51 | -1.11 | 0.42 | -1.76 | -3.30 | 1.42 | 2.26 | 4.78 | 0.76 |
Vacuole | 6.35 | -5.29 | -17.36 | -21.87 | -7.44 | -3.21 | -6.64 | -17.51 | -3.37 | -12.58 | 4.22 | -4.23 | -22.30 | -25.34 | -18.18 |
Vacuole Periphery | -5.63 | 2.00 | -0.94 | 3.81 | -2.45 | -0.68 | 11.19 | 6.77 | 1.44 | -3.20 | -4.20 | 7.32 | 2.08 | 5.49 | -4.01 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|