Standard name
Description Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; may control nonselective autophagy and mitophagy through trafficking of Atg9p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.99 0.99 0.97 0.98 0.92 0.94 0.86 0.79 0.68 1.0 0.97 0.96 0.94 0.96 0.74 0.79 0.6 0.84 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.06 0 0.09 0.16 0.16 0.3 0.39 0.43 0 0 0 0 0 0.15 0.09 0.22 0.06 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 5 1 3 2
Bud 1 1 1 2 1 1 10 2 4 7 5 0 0 11 0 1 0 1 0 0 0 3
Bud Neck 4 1 1 3 11 4 9 5 3 7 5 0 0 0 4 6 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 1 1 1 0 3 0 0 0 0 0
Cell Periphery 0 1 3 1 3 3 1 3 2 0 2 0 1 0 3 3 2 2 4 0 1 1
Cytoplasm 212 250 195 246 311 384 392 461 232 215 240 150 296 295 244 388 166 200 103 135 234 245
Endoplasmic Reticulum 1 1 0 0 1 0 0 0 0 0 1 0 10 9 9 28 1 0 2 0 2 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 5 3 1 0 0 0 1
Golgi 1 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0
Mitochondria 3 15 4 14 9 36 68 76 82 105 153 4 1 5 5 9 34 24 38 9 9 15
Nucleus 1 0 0 1 0 0 1 3 0 0 2 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 2 2 1 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 1 1 0 1 0 0 6 8 4 5 12 0 0 1 3 2 3 2 1 1 1 4
Vac/Vac Membrane 1 2 0 0 0 0 2 0 1 0 0 0 0 0 4 7 1 7 2 0 5 6
Unique Cell Count 218 260 196 249 322 393 425 489 269 271 353 150 306 306 260 404 225 254 172 161 268 292
Labelled Cell Count 226 273 204 268 337 429 491 561 331 340 428 154 309 321 279 450 225 254 172 161 268 292


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.1 2.9 2.6 2.9 2.6 2.5 2.7 2.2 2.3 2.1 3.9 4.2 3.7 4.7 5.1 5.1 3.9 3.4 4.4
Std Deviation (1e-4) 0.7 0.6 0.9 0.9 0.8 0.8 1.2 1.1 1.1 0.9 1.1 1.3 1.8 1.7 1.7 2.1 1.6 1.3 1.1 2.0
Intensity Change (Log2) -0.12 0.01 -0.13 -0.17 -0.1 -0.35 -0.34 -0.43 0.44 0.56 0.37 0.73 0.84 0.83 0.44 0.24 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pik1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available