Standard name
Human Ortholog
Description Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0.14 0 0.15 0.14 0.14 0.22
Bud 0 0 – – – 0 – 0 – – – 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0.06
Bud Site 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – – 0.05 – 0 – – – 0 0 0 0 0.05 0.1 0.09 0.1 0.12 0.05 0.14 0.13 0.17
Cytoplasm 0.89 0.96 – – – 0.84 – 0.75 – – – 0.59 0.97 0.97 0.98 0.84 0.79 0.84 0.34 0.53 0.36 0.41 0.47 0.29
Endoplasmic Reticulum 0 0 – – – 0 – 0 – – – 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0
Endosome 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – – 0.19 – 0.32 – – – 0.58 0 0 0 0.05 0 0.06 0.22 0.12 0.36 0.12 0.12 0.15
Nucleus 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 – – – 0 – 0 – – – 1 0 3 1 4 3 3 31 2 5 17 26 55
Bud 0 0 – – – 1 – 3 – – – 5 1 5 10 6 2 1 1 1 0 1 3 3
Bud Neck 4 0 – – – 0 – 0 – – – 1 1 0 0 4 2 0 10 1 0 8 6 15
Bud Site 0 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 2 – – – 2 – 4 – – – 2 5 1 7 9 10 10 22 9 2 16 24 42
Cytoplasm 168 45 – – – 31 – 69 – – – 47 134 233 216 139 79 90 74 43 14 50 88 75
Endoplasmic Reticulum 1 2 – – – 1 – 2 – – – 0 0 0 0 13 1 7 9 0 0 1 1 4
Endosome 0 0 – – – 0 – 1 – – – 0 0 0 0 3 5 1 5 2 0 4 2 4
Golgi 1 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 1 0 0 0 2 1
Mitochondria 1 0 – – – 7 – 29 – – – 46 2 2 6 8 1 6 47 9 13 14 21 38
Nucleus 4 0 – – – 0 – 0 – – – 1 0 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 13 0 – – – 0 – 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – – 0 – 0 – – – 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 – – – 0 – 0 – – – 0 0 1 0 0 2 2 0 0 0 0 0 1
SpindlePole 1 0 – – – 0 – 1 – – – 3 0 0 0 6 5 7 1 0 0 1 0 0
Vac/Vac Membrane 0 1 – – – 0 – 0 – – – 0 1 0 0 2 6 0 6 1 0 2 4 7
Unique Cell Count 189 47 37 92 79 138 241 221 165 100 107 218 81 39 123 186 257
Labelled Cell Count 201 50 42 109 106 144 245 240 195 119 127 218 81 39 123 186 257


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.9 – – – 3.2 – 3.1 – – – 2.8 3.9 4.2 3.7 5.8 6.9 6.0 4.1 4.2 4.6
Std Deviation (1e-4) 0.7 1.3 – – – 1.0 – 1.2 – – – 1.5 1.1 1.1 1.2 2.1 4.6 2.0 1.3 1.6 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6489 2.0495 1.9582 1.5822 1.1377 1.9796 0.8998 1.1158 1.3226 1.1211 0.64 1.4084 0.8708 1.3901 1.4039 1.0057 0.8307 1.7512
Actin 0.0015 0.0024 0.0146 0.0009 0.0002 0.0022 0.0593 0.0079 0.0086 0.0009 0.0067 0.001 0.1362 0.0073 0.0017 0.0034 0.0002 0.007
Bud 0.0028 0.0006 0.0009 0.0003 0 0.0001 0.0003 0.0019 0.0005 0.0003 0.0002 0 0.0029 0.0063 0.0008 0.0001 0 0.0005
Bud Neck 0.0055 0.0016 0.0179 0.0035 0.0003 0.0027 0.0104 0.0021 0.0106 0.0048 0.0386 0.0024 0.0013 0.001 0.0041 0.0163 0.0001 0.0231
Bud Periphery 0.0061 0.0017 0.0048 0.001 0.0002 0.0003 0.0009 0.0035 0.0017 0.0003 0.0012 0.0001 0.0071 0.0314 0.0019 0.0004 0 0.0022
Bud Site 0.0419 0.0354 0.0155 0.0021 0.0001 0.0001 0.0068 0.0377 0.0397 0.0026 0.0022 0.0001 0.1063 0.0667 0.0095 0.0005 0 0.0004
Cell Periphery 0.0141 0.0128 0.0194 0.0096 0.0027 0.0014 0.0043 0.0142 0.0096 0.003 0.0091 0.0005 0.0027 0.0025 0.0038 0.0047 0.0001 0.0019
Cytoplasm 0.4795 0.4997 0.5406 0.6041 0.4284 0.6051 0.1446 0.3903 0.4141 0.3909 0.3603 0.5256 0.1795 0.2677 0.179 0.3131 0.2766 0.3482
Cytoplasmic Foci 0.0199 0.0093 0.005 0.0056 0.0013 0.0058 0.0208 0.011 0.0123 0.0245 0.0393 0.0127 0.0471 0.0084 0.0053 0.0218 0.0024 0.0038
Eisosomes 0.0017 0.0005 0.0006 0.0002 0.0002 0.0002 0.0008 0.0008 0.0005 0.0003 0.0006 0 0.0005 0.0008 0.0006 0.0014 0 0.0003
Endoplasmic Reticulum 0.0034 0.005 0.0031 0.0035 0.0013 0.0019 0.0094 0.0018 0.0024 0.0051 0.0007 0.001 0.013 0.002 0.0023 0.0026 0.0006 0.0012
Endosome 0.0029 0.0009 0.0004 0.0011 0.0001 0.0009 0.0147 0.0052 0.0076 0.018 0.0003 0.0111 0.0238 0.0005 0.0228 0.0007 0.0004 0.0016
Golgi 0.0013 0.0005 0.0005 0.0004 0 0.0002 0.0138 0.0005 0.0144 0.0025 0.0005 0.002 0.015 0.0003 0.0006 0.0005 0 0.0002
Lipid Particles 0.0054 0.001 0.0009 0.0012 0.0002 0.0007 0.0345 0.0004 0.0014 0.0005 0.0002 0.0054 0.1236 0.0004 0.0017 0.0008 0 0.0001
Mitochondria 0.0017 0.0002 0.0003 0.0002 0 0.0001 0.0024 0.0002 0.0037 0.0003 0.0002 0.0013 0.0028 0.0008 0.0004 0.0002 0.0001 0.0002
None 0.4042 0.4253 0.3731 0.3637 0.5642 0.3771 0.6385 0.5181 0.4596 0.5417 0.5377 0.4121 0.2917 0.6011 0.7348 0.632 0.7189 0.6079
Nuclear Periphery 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0164 0.0002 0.0004 0.0003 0 0.0126 0.0012 0.0002 0.0007 0.0002 0.0001 0.0001
Nucleolus 0.0009 0.0002 0.0001 0.0001 0.0001 0.0001 0.0014 0.0001 0.0005 0.0001 0.0001 0.0003 0.0008 0.0003 0.0003 0.0001 0 0.0001
Nucleus 0.0012 0.0006 0.0005 0.0004 0.0002 0.0003 0.0086 0.0005 0.0013 0.0006 0.0002 0.0042 0.0006 0.0004 0.0006 0.0003 0.0001 0.0003
Peroxisomes 0.003 0.0001 0.0002 0.0001 0 0.0001 0.0082 0.0003 0.0069 0.0003 0.001 0.0026 0.0387 0.0009 0.0003 0.0002 0 0.0001
Punctate Nuclear 0.0009 0.0004 0.0004 0.0002 0 0.0002 0.0017 0.0002 0.0005 0.0003 0.0004 0.0041 0.004 0.0005 0.0006 0.0003 0.0001 0.0001
Vacuole 0.0018 0.0016 0.0011 0.0015 0.0006 0.0004 0.0016 0.003 0.0035 0.0023 0.0005 0.0004 0.0009 0.0005 0.0272 0.0005 0.0003 0.0005
Vacuole Periphery 0.0001 0 0 0.0001 0 0 0.0005 0.0001 0.0002 0.0002 0 0.0003 0.0004 0 0.001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.6696 11.4944 12.8931 18.7919 16.8649 15.8396 21.6898 21.6167 18.1873 24.4824
Translational Efficiency 0.2215 0.432 0.3582 0.2663 0.2956 0.3537 0.284 0.2117 0.3218 0.2658

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1988 14 134 154 1408 1086 304 60 3396 1100 438 214

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.26 725.54 988.14 875.26 639.17 728.95 913.67 843.57 633.37 728.91 936.45 866.37
Standard Deviation 72.58 116.56 107.91 86.80 84.08 93.13 87.64 93.64 77.71 93.46 100.36 89.90
Intensity Change Log 2 — 0.205399 0.651059 0.476055 — 0.189620 0.515473 0.400308 — 0.197469 0.584329 0.438383

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000317 0.000440 0.011834 0.017636 0.001901 0.002642 0.001854 0.016105 0.000974 0.002614 0.004907 0.017207
Bud Neck 0.026452 0.009283 0.009381 0.011132 0.038914 0.047336 0.007610 0.005310 0.031619 0.046852 0.008152 0.009500
Bud Site 0.009083 0.004873 0.040347 0.047430 0.009586 0.022444 0.021271 0.013632 0.009292 0.022220 0.027107 0.037954
Cell Periphery 0.000446 0.000329 0.005039 0.001054 0.001160 0.000879 0.001678 0.003747 0.000742 0.000872 0.002706 0.001809
Cytoplasm 0.481395* 0.369352* 0.168000* 0.282833* 0.438316* 0.405812* 0.228263* 0.402019* 0.463535* 0.405348* 0.209827* 0.316249*
Cytoplasmic Foci 0.171274 0.363071* 0.088867 0.092434 0.251376* 0.249435* 0.061185 0.125098 0.204485* 0.250881* 0.069654 0.101592
Eisosomes 0.000761 0.000219 0.001203 0.000537 0.003738 0.001481 0.000566 0.002802 0.001995 0.001465 0.000761 0.001172
Endoplasmic Reticulum 0.000980 0.000571 0.024780 0.003403 0.002352 0.001106 0.007624 0.002049 0.001549 0.001099 0.012872 0.003023
Endosome 0.010332 0.014986 0.009961 0.015474 0.009786 0.020591 0.004829 0.008476 0.010106 0.020520 0.006399 0.013512
Golgi 0.002203 0.005184 0.000396 0.001008 0.007165 0.010174 0.000245 0.000701 0.004260 0.010111 0.000291 0.000922
Lipid Particles 0.011343 0.020853 0.010277 0.005947 0.022903 0.013525 0.008814 0.007003 0.016136 0.013618 0.009261 0.006243
Mitochondria 0.005234 0.025031 0.000301 0.004397 0.007316 0.010356 0.004838 0.000314 0.006097 0.010543 0.003450 0.003253
Mitotic Spindle 0.001017 0.000557 0.000216 0.005530 0.000181 0.012215 0.000591 0.001845 0.000670 0.012066 0.000477 0.004497
None 0.006215 0.016656 0.000428 0.000765 0.002398 0.002631 0.001557 0.000836 0.004633 0.002809 0.001212 0.000785
Nuclear Periphery 0.000338 0.000370 0.001536 0.000829 0.000301 0.000404 0.001242 0.000545 0.000323 0.000404 0.001332 0.000750
Nuclear Periphery Foci 0.000246 0.000538 0.008195 0.002128 0.000361 0.000529 0.002302 0.001031 0.000294 0.000529 0.004105 0.001821
Nucleolus 0.001305 0.003602 0.000488 0.000467 0.001290 0.001429 0.000487 0.000223 0.001299 0.001457 0.000487 0.000398
Nucleus 0.211216* 0.085599 0.351085* 0.143145* 0.147695* 0.120434 0.512333* 0.168934* 0.184880* 0.119991 0.463001* 0.150376*
Peroxisomes 0.005011 0.020470 0.002310 0.024271 0.017921 0.022018 0.001836 0.023626 0.010364 0.021999 0.001981 0.024090
Vacuole 0.054095 0.056378 0.265133* 0.336736* 0.034470 0.052381 0.130626 0.215496* 0.045959 0.052432 0.171776 0.302743*
Vacuole Periphery 0.000735 0.001637 0.000225 0.002843 0.000871 0.002179 0.000247 0.000210 0.000791 0.002172 0.000241 0.002105

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN -0.79 0.34 -1.29 -1.19 -1.32 -1.86 -2.66 -1.85 -1.50 -1.23
Bud Neck NaN NaN NaN NaN NaN -1.97 10.71 12.67 12.32 1.80 -4.18 13.55 13.47 11.16 0.32
Bud Site NaN NaN NaN NaN NaN -4.80 -1.59 -0.91 2.33 0.81 -5.16 -3.08 -2.81 -0.72 -0.63
Cell Periphery NaN NaN NaN NaN NaN 2.12 -1.63 -1.05 -1.22 -0.82 -1.14 -4.72 -1.40 -1.12 1.49
Cytoplasm NaN NaN NaN NaN NaN 3.33 18.19 2.29 1.32 -4.09 6.78 28.76 10.32 6.80 -3.78
Cytoplasmic Foci NaN NaN NaN NaN NaN 0.25 25.86 7.17 6.96 -3.14 -6.69 25.10 12.62 15.30 -2.08
Eisosomes NaN NaN NaN NaN NaN 4.93 7.49 1.05 -0.43 -1.07 1.98 6.05 2.29 1.16 -0.59
Endoplasmic Reticulum NaN NaN NaN NaN NaN 2.71 -5.58 -0.02 -2.91 5.63 1.75 -10.31 -5.39 -6.42 7.66
Endosome NaN NaN NaN NaN NaN -6.57 5.13 0.71 4.51 -1.13 -6.60 4.52 0.11 3.96 -1.34
Golgi NaN NaN NaN NaN NaN -2.29 14.76 12.01 7.77 -1.11 -4.77 15.32 11.95 7.93 -1.82
Lipid Particles NaN NaN NaN NaN NaN 6.39 6.79 8.49 4.08 0.93 2.32 4.91 8.91 6.12 1.91
Mitochondria NaN NaN NaN NaN NaN -1.76 1.19 6.23 7.54 0.98 -3.06 1.62 3.01 4.72 0.27
Mitotic Spindle NaN NaN NaN NaN NaN -5.05 -1.27 -1.03 3.51 -0.81 -4.80 0.59 -1.74 3.06 -1.90
None NaN NaN NaN NaN NaN -0.31 1.70 2.84 2.11 1.17 2.02 6.05 7.03 2.60 1.00
Nuclear Periphery NaN NaN NaN NaN NaN -1.32 -14.72 -3.02 -1.94 4.72 -1.09 -17.14 -7.36 -5.26 3.47
Nuclear Periphery Foci NaN NaN NaN NaN NaN -1.32 -7.40 -3.78 -0.87 4.57 -1.62 -10.57 -5.03 -3.07 3.62
Nucleolus NaN NaN NaN NaN NaN -0.38 1.54 2.04 3.98 3.60 -0.65 3.48 3.88 3.62 1.40
Nucleus NaN NaN NaN NaN NaN 4.30 -21.39 -1.79 -2.65 8.48 11.51 -19.19 0.89 -3.37 14.16
Peroxisomes NaN NaN NaN NaN NaN -1.80 11.28 -0.05 0.31 -1.49 -6.08 12.10 -0.78 1.30 -2.37
Vacuole NaN NaN NaN NaN NaN -6.17 -12.24 -7.04 -6.41 -2.58 -2.35 -16.04 -15.53 -15.15 -7.25
Vacuole Periphery NaN NaN NaN NaN NaN -3.07 4.20 4.05 4.76 0.05 -3.42 6.57 -0.45 1.13 -1.16
Standard name
Human Ortholog
Description Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p
Localization
Cell Percentages cytoplasm (16%), ER (5%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sip3

Sip3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sip3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available