Standard name
Human Ortholog
Description Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.99 0.97 0.96 – 0.86 0.85 0.74 0.86 0.76 1.0 0.99 1.0 0.99 1.0 0.99 0.98 0.95 0.96 0.93 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.09 0.08 0.15 0.09 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 – 4 4 11 0 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 – 1 0 2 0 3 0 0 1 0 0 1 0 1 0 0 2 2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 1 7 10 – 14 12 17 6 23 0 10 8 8 1 2 0 0 0 0 0 0
Cytoplasm 400 188 167 142 222 310 – 356 206 323 191 269 151 381 499 281 205 200 384 182 153 142 275 355
Endoplasmic Reticulum 1 2 1 1 5 9 – 37 20 66 21 51 0 2 4 9 8 9 0 2 1 3 2 4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 1 1 8 2 7 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 0 1 – 4 9 15 10 24 0 0 0 0 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 2 2 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Nuclear Periphery 0 0 0 0 0 0 – 1 1 3 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 1 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 1 – 5 4 17 1 3 1 0 1 1 0 2 0 0 0 0 6 5
Unique Cell Count 401 190 167 143 228 323 414 243 436 222 355 151 383 501 283 206 202 391 191 160 153 296 376
Labelled Cell Count 402 192 171 144 235 332 424 262 465 231 396 152 393 513 299 215 215 391 191 160 153 296 376


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 68.1 81.8 36.7 47.6 43.3 32.7 – 29.2 27.0 26.2 26.1 24.7 67.5 67.7 64.2 87.5 85.6 86.7 66.4 69.4 70.1
Std Deviation (1e-4) 12.6 17.0 9.0 11.8 8.4 6.6 – 6.8 7.6 6.6 7.2 6.0 15.3 14.6 13.8 26.0 27.1 23.7 16.9 20.5 19.0
Intensity Change (Log2) – – – 0.38 0.24 -0.17 – -0.33 -0.44 -0.49 -0.49 -0.57 0.88 0.88 0.81 1.25 1.22 1.24 0.86 0.92 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 2.5 0 0 3.0 0 0 0 0 0 0
Cytoplasm -1.1 -2.1 -2.6 – -5.1 -5.3 -7.3 -5.0 -7.0 0 -0.9 -0.8 -1.1 -0.9 -1.3
Endoplasmic Reticulum 0 0 0 – 3.7 3.4 5.1 3.7 4.9 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 53.7253 65.4042 60.9704 57.1948 48.9921 58.8217 56.4684 72.2576 65.3228 63.6022 56.5655 60.5939 68.4076 83.4781 77.0548 73.8614 75.9977 71.8434
Actin 0.0039 0.0006 0.0013 0.0002 0.0005 0.0008 0.0163 0.0001 0.0032 0.0002 0.0002 0.0398 0.0006 0 0.0002 0.0346 0 0.0004
Bud 0.0003 0.0003 0.0002 0.0005 0.0007 0.0007 0.0011 0.0002 0.0005 0.0002 0 0.0005 0.0011 0 0.0002 0.0002 0 0.0001
Bud Neck 0.0003 0.0002 0.0002 0.0005 0.0003 0.0009 0.0011 0.0001 0.0002 0.0002 0.0005 0.0017 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002
Bud Periphery 0.0003 0.0002 0.0002 0.0008 0.0005 0.0003 0.0007 0.0001 0.0006 0 0 0.0009 0.001 0 0.0001 0.0001 0 0.0002
Bud Site 0.0004 0.0005 0.0003 0.0001 0.0007 0.0001 0.0035 0.0001 0.0007 0 0 0.0029 0.0003 0 0.0001 0.0005 0 0.0001
Cell Periphery 0.0003 0.0005 0.0002 0.0007 0.0013 0.0001 0.0007 0.0001 0.0003 0 0 0.0004 0.0004 0 0.0001 0 0 0.0002
Cytoplasm 0.8794 0.9901 0.9622 0.9277 0.8633 0.9376 0.8801 0.9886 0.973 0.986 0.8667 0.9155 0.9201 0.9983 0.9668 0.9429 0.9894 0.8649
Cytoplasmic Foci 0.011 0.0006 0.0058 0.0011 0.0117 0.0032 0.0132 0.0017 0.0022 0.0011 0.0004 0.0028 0.0164 0.0001 0.0025 0.0015 0.0006 0.0107
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0 0 0.001 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.02 0.0029 0.0029 0.0131 0.0069 0.0104 0.0128 0.001 0.0028 0.0002 0.0026 0.0099 0.01 0.0003 0.003 0.0006 0.0063 0.0287
Endosome 0.0074 0.0003 0.0041 0.0039 0.0117 0.0117 0.0131 0.0012 0.003 0.0016 0.0068 0.0063 0.0034 0.0001 0.0013 0.0011 0.0006 0.0038
Golgi 0.0039 0.0001 0.0008 0.0001 0.0009 0.0009 0.0042 0.0001 0.0011 0 0.0001 0.0021 0.0005 0 0.0005 0.0007 0 0.0002
Lipid Particles 0.0067 0.0001 0.0021 0.0006 0.0045 0.0001 0.0037 0.0006 0.0009 0 0.0001 0.0016 0.0062 0 0.0056 0.0006 0 0.0027
Mitochondria 0.0048 0.0001 0.0014 0.0021 0.0146 0.0062 0.0043 0.0004 0.0034 0 0.0003 0.0049 0.0038 0 0.0072 0.0002 0 0.0044
None 0.0037 0.0002 0.0004 0.0004 0.0076 0.0004 0.0026 0.0004 0.0008 0.0001 0.0003 0.0009 0.0032 0 0.0012 0.0001 0.0001 0.0051
Nuclear Periphery 0.0178 0.0013 0.0036 0.0103 0.0041 0.003 0.0134 0.0006 0.0012 0.0008 0.0992 0.002 0.0083 0.0002 0.001 0.0023 0.0009 0.0169
Nucleolus 0.0043 0 0.0001 0.0001 0.0091 0.0002 0.0008 0.0002 0.0004 0 0.0001 0.0001 0.0033 0 0.002 0 0 0.0057
Nucleus 0.0104 0.0008 0.0019 0.005 0.0068 0.0027 0.0065 0.0007 0.0011 0.0013 0.0149 0.0017 0.0047 0.0004 0.0033 0.0015 0.0009 0.0363
Peroxisomes 0.0024 0 0.0035 0 0.0008 0.0001 0.0017 0.0001 0.0003 0 0 0.001 0.0008 0 0.0009 0.0033 0 0.0002
Punctate Nuclear 0.005 0.0001 0.0011 0.0001 0.0006 0.0001 0.0011 0.0001 0.0001 0 0.0016 0.0003 0.004 0 0.0006 0.0003 0 0.0036
Vacuole 0.0115 0.0008 0.0056 0.0196 0.0311 0.0127 0.0143 0.0029 0.0028 0.0064 0.0039 0.0022 0.0083 0.0002 0.0023 0.0072 0.0008 0.0083
Vacuole Periphery 0.0063 0.0001 0.0018 0.0134 0.0221 0.008 0.0045 0.0007 0.0014 0.0017 0.0022 0.0015 0.0035 0 0.001 0.0019 0.0002 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 421.5387 441.9478 400.8208 323.3564 360.7496 381.9199 363.2029 313.98 303.4529 325.4371
Translational Efficiency 2.5441 2.476 2.4158 2.2336 2.7358 2.8765 2.3054 2.8251 2.4712 2.7261

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2067 469 157 1360 1545 1908 239 1634 3612 2377 396 2994

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3557.23 4485.35 5566.86 6382.46 3134.18 4376.69 6415.74 6132.19 3376.27 4398.13 6079.19 6245.87
Standard Deviation 703.88 1081.18 1080.23 1376.52 775.11 928.43 964.60 1390.59 764.41 961.47 1093.90 1389.82
Intensity Change Log 2 — 0.334466 0.646110 0.843358 — 0.481752 1.033528 0.968314 — 0.405329 0.840559 0.903236

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000030 0.000177 0.000183 0.000405 0.000088 0.000055 0.000133 0.000223 0.000055 0.000079 0.000153 0.000306
Bud Neck 0.000232 0.001368 0.005492 0.001554 0.000388 0.000360 0.003321 0.001944 0.000299 0.000559 0.004182 0.001767
Bud Site 0.001365 0.007008 0.002594 0.008281 0.002067 0.001526 0.003535 0.007762 0.001665 0.002608 0.003162 0.007998
Cell Periphery 0.001167 0.013548 0.021847 0.021263 0.004700 0.001727 0.051532 0.010411 0.002678 0.004059 0.039763 0.015341
Cytoplasm 0.902723* 0.787094* 0.465126* 0.766443* 0.820540* 0.916217* 0.522185* 0.769379* 0.867570* 0.890740* 0.499563* 0.768045*
Cytoplasmic Foci 0.007559 0.017606 0.044463 0.003270 0.006688 0.005815 0.016373 0.003705 0.007187 0.008142 0.027509 0.003508
Eisosomes 0.000000 0.000003 0.000007 0.000005 0.000001 0.000001 0.000006 0.000004 0.000001 0.000001 0.000006 0.000005
Endoplasmic Reticulum 0.000381 0.000347 0.000150 0.000160 0.000864 0.000349 0.000106 0.000158 0.000588 0.000348 0.000124 0.000159
Endosome 0.000532 0.001833 0.000904 0.000278 0.000542 0.000385 0.000492 0.000726 0.000537 0.000671 0.000655 0.000522
Golgi 0.024167 0.033382 0.054252 0.004496 0.039280 0.018018 0.019729 0.005490 0.030632 0.021050 0.033416 0.005038
Lipid Particles 0.002540 0.015106 0.015482 0.017464 0.005361 0.003006 0.037048 0.009968 0.003746 0.005394 0.028498 0.013373
Mitochondria 0.000070 0.000247 0.000212 0.000634 0.000180 0.000109 0.000055 0.000407 0.000117 0.000136 0.000117 0.000510
Mitotic Spindle 0.001418 0.004228 0.002288 0.000707 0.000548 0.001003 0.001733 0.001104 0.001046 0.001639 0.001953 0.000924
None 0.001487 0.003440 0.002593 0.001979 0.002473 0.001867 0.001311 0.001971 0.001908 0.002177 0.001820 0.001975
Nuclear Periphery 0.000163 0.000465 0.003445 0.000252 0.000239 0.000220 0.001328 0.000209 0.000195 0.000268 0.002167 0.000229
Nuclear Periphery Foci 0.007212 0.009541 0.018448 0.003507 0.009188 0.007027 0.012604 0.003452 0.008057 0.007523 0.014921 0.003477
Nucleolus 0.000040 0.000121 0.000254 0.000128 0.000160 0.000063 0.000088 0.000303 0.000091 0.000074 0.000154 0.000223
Nucleus 0.007629 0.017228 0.066702 0.023280 0.013666 0.011404 0.033283 0.017060 0.010211 0.012553 0.046533 0.019886
Peroxisomes 0.000008 0.000115 0.000019 0.000066 0.000012 0.000007 0.000015 0.000087 0.000009 0.000028 0.000017 0.000077
Vacuole 0.021238 0.067218 0.233092* 0.139896 0.043175 0.021611 0.281284* 0.157192 0.030621 0.030610 0.262178* 0.149335
Vacuole Periphery 0.020038 0.019927 0.062445 0.005931 0.049840 0.009229 0.013838 0.008445 0.032785 0.011339 0.033109 0.007303

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.07 -7.16 -3.44 -1.95 -1.71 1.83 -3.12 -3.02 -3.93 -1.48 -2.39 -8.18 -4.49 -3.97 -2.23
Bud Neck -6.20 -7.57 -8.02 -1.05 5.70 0.07 -7.46 -6.64 -7.00 2.97 -4.46 -10.52 -9.97 -8.20 6.27
Bud Site -7.06 -1.99 -7.87 -1.04 -2.45 2.05 -2.93 -5.88 -6.72 -3.90 -3.64 -3.46 -9.64 -8.06 -4.56
Cell Periphery -6.86 -9.11 -14.48 -2.78 0.93 7.42 -8.74 -5.72 -10.52 7.80 -3.29 -10.99 -15.72 -12.36 7.33
Cytoplasm 9.43 18.62 18.66 1.98 -12.09 -12.96 14.81 8.91 22.24 -10.93 -3.52 24.00 20.38 22.20 -16.03
Cytoplasmic Foci -5.81 -7.12 6.65 7.82 7.68 1.18 -4.74 2.92 2.26 5.39 -2.19 -8.26 6.44 7.48 9.13
Eisosomes -4.57 -8.06 -6.29 -2.31 2.15 3.07 -7.36 -4.10 -4.75 2.87 -2.86 -10.91 -7.39 -6.47 3.52
Endoplasmic Reticulum 0.37 2.34 2.40 2.92 0.24 2.51 4.71 4.38 1.55 -1.04 1.79 5.24 4.90 1.90 -0.91
Endosome -1.94 -4.00 4.03 2.33 6.04 2.46 0.16 -1.28 -2.40 -1.25 -0.98 -2.60 0.29 1.04 2.21
Golgi -2.83 -6.33 14.40 10.31 11.92 9.30 8.26 17.04 11.02 8.49 6.09 -0.17 22.03 15.06 13.49
Lipid Particles -7.49 -8.81 -12.61 -1.24 0.21 3.16 -10.74 -5.26 -9.31 9.07 -3.35 -13.15 -13.56 -10.46 7.88
Mitochondria -1.48 -6.04 -1.51 -0.88 -1.00 2.20 5.02 -2.04 -2.68 -3.23 -0.74 -0.69 -2.26 -2.08 -2.19
Mitotic Spindle -1.54 -2.09 1.98 1.93 3.19 -3.35 -1.86 -1.28 -0.39 0.71 -1.51 -2.30 0.27 1.43 2.15
None -1.82 -2.86 -1.13 1.36 0.86 1.85 3.75 1.09 -0.03 -1.25 -1.03 0.99 -0.02 0.74 -0.59
Nuclear Periphery -3.99 -9.25 -4.36 2.70 8.97 0.19 -10.70 -0.52 -0.50 10.51 -2.49 -12.31 -3.54 0.62 11.93
Nuclear Periphery Foci -2.36 -5.73 5.92 5.95 7.78 2.37 -2.83 8.35 5.71 8.08 0.40 -6.40 9.74 7.87 11.10
Nucleolus -3.45 -8.82 -6.97 0.72 5.01 2.14 1.53 -2.36 -4.61 -4.19 0.40 -3.41 -4.08 -4.61 -2.11
Nucleus -5.02 -8.76 -13.18 -3.08 5.71 1.02 -5.95 -5.50 -7.21 3.83 -3.34 -10.60 -13.72 -9.40 6.58
Peroxisomes -1.77 -4.22 -6.90 0.87 -4.07 1.76 -1.34 -5.21 -5.64 -4.90 -1.58 -4.38 -7.86 -2.96 -6.37
Vacuole -8.06 -11.69 -22.53 -9.66 4.81 7.02 -14.30 -22.09 -26.65 6.20 -0.62 -18.75 -32.19 -30.52 7.96
Vacuole Periphery 0.19 -4.61 9.24 5.43 6.71 14.55 12.59 15.50 1.67 3.88 12.82 0.97 17.44 5.25 7.16
Standard name
Human Ortholog
Description Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Gis2

Gis2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gis2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available