Standard name
Human Ortholog
Description RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.11 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.37 0.15 0.23 0.15 0.14 0.11 0.05 0 0.08 0 0.05 0.06 0 0 0.13 0.07 0.07 0 0 0 0 0 0
Nucleus 0.07 0.09 0 0.05 0.09 0.05 0 0 0.05 0.07 0 0 0.07 0.06 0.09 0.06 0.06 0.08 0.06 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.98 0.95 0.93 0.92 0.9 0.94 0.91 0.89 0.83 0.84 0.9 0.91 0.87 0.85 0.77 0.87 0.93 0.88 0.83 0.9 0.8 0.84 0.78 0.67
Peroxisomes 0 0 0 0 0 0 0 0.09 0.07 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.17 0.22 0.31 0.42 0.28 0.41 0.43 0.06 0.09 0.12 0 0 0 0.09 0 0.12 0.06 0.09 0.16
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 1 1 2 2 2 3 0 0 0 0 0 0 0 0 0 0 1 2 1 1
Bud Neck 1 3 4 0 3 2 12 4 10 10 7 8 3 1 4 0 0 0 1 0 4 1 3 6
Bud Site 0 0 0 0 4 6 10 6 11 7 11 12 1 0 3 3 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1 6 1 4 3 4 2 3 4 3 0 22 37 47 13 7 16 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 3 0 0 0 1 0 2
Endosome 0 0 0 0 2 0 0 4 1 0 1 0 3 6 10 2 1 3 0 0 1 2 3 5
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Mitochondria 34 83 34 38 30 37 39 15 11 18 12 9 22 4 16 39 21 20 0 2 1 1 4 8
Nucleus 14 21 8 9 18 14 8 8 13 15 12 6 25 20 38 20 17 24 12 11 4 7 13 9
Nuclear Periphery 0 1 0 0 0 0 0 1 0 0 1 0 0 0 3 0 0 0 0 0 1 0 2 7
Nucleolus 205 211 214 155 182 248 315 247 211 178 241 168 310 302 334 269 264 255 175 201 185 209 242 236
Peroxisomes 2 2 6 0 3 11 14 24 18 11 16 11 9 4 9 3 6 4 0 0 1 0 0 2
SpindlePole 7 1 8 6 9 45 76 85 107 60 109 79 23 33 52 5 3 3 19 4 28 14 27 54
Vac/Vac Membrane 0 0 0 0 1 0 2 0 0 0 0 0 1 0 1 3 3 2 0 1 1 4 5 9
Unique Cell Count 210 223 229 168 203 265 348 276 255 212 268 184 358 355 432 308 285 291 211 225 233 250 310 352
Labelled Cell Count 263 323 281 210 257 367 483 398 387 306 413 293 419 407 517 357 324 330 211 225 233 250 310 352


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.2 26.1 20.4 18.4 13.8 11.1 10.1 9.4 8.7 8.2 7.6 7.3 23.5 22.6 18.1 31.3 30.9 30.1 24.2 25.8 24.6
Std Deviation (1e-4) 5.0 8.7 6.0 5.9 3.9 3.2 3.1 2.8 2.4 2.5 2.5 2.4 8.8 11.2 8.0 14.4 13.5 14.0 8.5 9.6 8.9
Intensity Change (Log2) – – – -0.15 -0.56 -0.87 -1.01 -1.12 -1.23 -1.31 -1.43 -1.48 0.2 0.15 -0.17 0.62 0.6 0.56 0.25 0.34 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 1.2 0 1.4 1.8 0.7 1.6 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.2 0 3.1 3.9 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.0 3.5 3.7 1.0 -0.1 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 0 -0.3 -1.3 -3.6 -4.0 -2.1 -4.0 -3.3 -3.5 -6.6 -5.2 -0.7 -2.7 -3.0
Nucleus 0.9 2.3 1.0 -0.9 -0.4 0.9 1.7 0.6 -0.1 1.8 1.2 2.5 1.5 1.3 2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 -1.4 0.1 -1.2 -1.6 -3.6 -3.2 -1.4 -0.8 -2.6 -3.1 -5.2 -2.3 -0.4 -2.2
Peroxisomes 0 0 0.9 0.9 2.9 2.2 1.4 1.8 1.7 -0.1 0 -0.4 0 -0.4 0
SpindlePole 0 0.5 4.8 6.1 7.9 9.9 7.2 9.7 9.8 1.5 2.7 3.6 -1.4 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.8823 19.7259 18.6116 17.5109 17.0043 16.871 12.7939 21.7345 19.3166 16.3816 20.2014 19.1136 17.5858 19.7657 19.7625 19.7295 11.6797 18.2429
Actin 0.0174 0 0.0001 0 0.0395 0.0076 0.039 0.001 0.0005 0 0 0.0012 0.0038 0 0.0016 0 0.0026 0.018
Bud 0.0001 0 0 0 0.0001 0.0001 0.0004 0.0001 0 0.0001 0 0 0.0001 0 0 0 0.0001 0.0001
Bud Neck 0.0003 0.0001 0 0 0.0014 0.0001 0.0006 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0039
Bud Periphery 0.0003 0 0 0 0.0001 0.0002 0.0005 0.0001 0 0 0 0.0001 0.0001 0 0 0 0.0002 0.0002
Bud Site 0.0007 0 0.0001 0 0.0001 0.0001 0.0016 0.0004 0.0001 0.0002 0.0001 0.0001 0.0008 0.0001 0.0007 0 0.0004 0.0002
Cell Periphery 0.0008 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0013 0.0005 0.0001 0.0003 0.0001 0.0002 0.0002 0.0004 0.0003
Cytoplasm 0.0016 0 0.0002 0 0 0.0001 0.0064 0.0004 0.0003 0.0001 0 0 0.0036 0 0.0024 0 0.0001 0
Cytoplasmic Foci 0.0141 0.0012 0.0077 0.0004 0.0012 0.0025 0.0227 0.0026 0.006 0.0891 0.0004 0.003 0.0257 0.0043 0.0096 0.0099 0.0202 0.0014
Eisosomes 0.0015 0 0.0002 0 0.0001 0.0001 0.0003 0 0 0.0001 0 0 0.0002 0 0 0 0.0004 0.0001
Endoplasmic Reticulum 0.0065 0 0 0 0 0.0002 0.0062 0.0001 0.0001 0 0 0 0.0013 0 0.0011 0 0.0002 0
Endosome 0.0054 0 0 0 0.0003 0.002 0.0175 0.0014 0.003 0.0001 0 0.0017 0.0154 0 0.0044 0 0.0065 0.0001
Golgi 0.0038 0 0.0001 0 0.0013 0.0014 0.0049 0.001 0.0055 0.0001 0 0.0041 0.0042 0 0.0009 0 0.0202 0.0004
Lipid Particles 0.0131 0.0019 0.0035 0.0049 0.0014 0.0061 0.034 0.0004 0.0123 0.0181 0.0016 0.0029 0.036 0.0005 0.0033 0.0105 0.2135 0.0045
Mitochondria 0.0105 0.0001 0.006 0.0126 0.0064 0.0013 0.0179 0.0004 0.0086 0.0117 0.0362 0.0039 0.0048 0.0004 0.0074 0.0033 0.1241 0.0006
None 0.0117 0 0.0011 0 0 0.0002 0.0055 0 0.0002 0.0008 0 0 0.0034 0 0.0006 0.0001 0.0004 0
Nuclear Periphery 0.0026 0.0039 0.0002 0 0 0.0029 0.0186 0 0.0002 0.0001 0 0 0.0039 0 0.0006 0 0.0004 0
Nucleolus 0.8623 0.9262 0.9247 0.9073 0.906 0.9263 0.7286 0.9212 0.8955 0.8214 0.9232 0.9223 0.8409 0.9504 0.9147 0.9448 0.5715 0.9309
Nucleus 0.0347 0.0609 0.0522 0.0734 0.0403 0.0424 0.0492 0.0685 0.0625 0.0472 0.0362 0.0526 0.0334 0.0419 0.0488 0.0287 0.019 0.0377
Peroxisomes 0.0036 0 0.0006 0.0003 0.0005 0.0007 0.0119 0.0003 0.0021 0.0025 0.0009 0.005 0.0066 0.0001 0.001 0.0003 0.0152 0.0002
Punctate Nuclear 0.0072 0.0052 0.0021 0.0002 0.0008 0.005 0.0288 0.0014 0.0024 0.0038 0.0001 0.0027 0.0133 0.002 0.0021 0.0004 0.0016 0.001
Vacuole 0.0009 0.0001 0.0003 0.0001 0.0001 0.0002 0.0033 0.0004 0.0003 0.0019 0.0003 0.0001 0.0008 0.0001 0.0004 0.0003 0.0008 0.0002
Vacuole Periphery 0.0006 0.0002 0.0006 0.0006 0.0003 0.0004 0.0016 0.0003 0.0003 0.0011 0.0004 0.0001 0.0016 0 0.0002 0.0012 0.0021 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 109.6331 98.7957 75.6566 72.6803 97.4375 83.9466 66.3284 55.2848 50.8186 73.7982
Translational Efficiency 2.1117 1.9621 2.2434 1.7546 1.7604 1.9308 1.8732 1.7291 1.767 1.8125

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
101 300 495 1781 1779 1751 349 1390 1880 2051 844 3171

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1682.60 1998.63 2130.09 2436.83 1581.93 1858.55 2281.14 2307.26 1587.34 1879.04 2192.55 2380.03
Standard Deviation 438.41 513.68 465.47 662.24 390.90 440.90 476.64 581.22 394.25 454.98 475.97 631.30
Intensity Change Log 2 — 0.248319 0.340222 0.534313 — 0.232492 0.528069 0.544495 — 0.240671 0.434306 0.539256

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001021 0.000255 0.000349 0.000578 0.000214 0.000382 0.000280 0.000511 0.000257 0.000364 0.000320 0.000548
Bud Neck 0.001668 0.001141 0.002810 0.003918 0.000938 0.001924 0.001779 0.005470 0.000977 0.001810 0.002384 0.004598
Bud Site 0.000814 0.000608 0.000810 0.001512 0.000357 0.000591 0.000667 0.001672 0.000382 0.000593 0.000750 0.001582
Cell Periphery 0.000081 0.000032 0.000024 0.000075 0.000013 0.000022 0.000013 0.000081 0.000016 0.000024 0.000019 0.000077
Cytoplasm 0.000429 0.000442 0.000071 0.000472 0.000127 0.000245 0.000030 0.000368 0.000143 0.000274 0.000054 0.000427
Cytoplasmic Foci 0.016620 0.014287 0.006843 0.008487 0.009051 0.009341 0.009959 0.006609 0.009457 0.010064 0.008131 0.007664
Eisosomes 0.000223 0.000277 0.000199 0.000219 0.000489 0.000521 0.000244 0.000201 0.000475 0.000485 0.000218 0.000211
Endoplasmic Reticulum 0.002989 0.000064 0.000164 0.000129 0.000088 0.000127 0.000066 0.000163 0.000244 0.000118 0.000123 0.000144
Endosome 0.001092 0.000029 0.000030 0.001136 0.000012 0.000113 0.000014 0.000723 0.000070 0.000101 0.000023 0.000955
Golgi 0.001824 0.000169 0.000394 0.000823 0.000153 0.000340 0.000375 0.000534 0.000242 0.000315 0.000386 0.000696
Lipid Particles 0.001634 0.000040 0.000016 0.000118 0.000021 0.000205 0.000017 0.000070 0.000107 0.000181 0.000016 0.000097
Mitochondria 0.001346 0.001042 0.001531 0.003334 0.001126 0.001488 0.001638 0.002554 0.001138 0.001423 0.001575 0.002992
Mitotic Spindle 0.013406 0.002552 0.041433 0.019159 0.007390 0.007206 0.023187 0.020724 0.007713 0.006525 0.033888 0.019845
None 0.000429 0.000962 0.000401 0.000601 0.000379 0.000395 0.000287 0.000658 0.000382 0.000478 0.000354 0.000626
Nuclear Periphery 0.000345 0.000099 0.000215 0.000329 0.000066 0.000111 0.000122 0.000391 0.000081 0.000109 0.000176 0.000356
Nuclear Periphery Foci 0.000918 0.000141 0.000086 0.000225 0.000107 0.000206 0.000072 0.000310 0.000151 0.000197 0.000080 0.000262
Nucleolus 0.551682* 0.547074* 0.589101* 0.669986* 0.540656* 0.607946* 0.565144* 0.647896* 0.541249* 0.599042* 0.579195* 0.660303*
Nucleus 0.129146 0.164001* 0.132749* 0.173172* 0.103414 0.152164* 0.082132 0.212975* 0.104796 0.153896* 0.111819 0.190619*
Peroxisomes 0.272917* 0.265926* 0.221912* 0.112060* 0.334811* 0.215571* 0.313127* 0.094621 0.331486* 0.222936* 0.259630* 0.104416*
Vacuole 0.000763 0.000385 0.000317 0.001400 0.000285 0.000513 0.000346 0.001600 0.000311 0.000494 0.000329 0.001487
Vacuole Periphery 0.000651 0.000475 0.000546 0.002267 0.000303 0.000589 0.000502 0.001869 0.000322 0.000573 0.000528 0.002093

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.03 0.89 0.58 -6.07 -6.67 -3.93 -2.80 -12.99 -2.91 -7.34 -2.03 -1.70 -7.01 -4.71 -9.64
Bud Neck 1.51 -2.55 -5.00 -9.35 -2.87 -4.91 -6.53 -9.07 -6.95 -7.44 -4.71 -8.11 -13.34 -9.38 -7.28
Bud Site 0.61 -0.18 -1.71 -2.51 -2.03 -3.21 -4.99 -6.36 -5.14 -4.90 -2.98 -6.40 -5.89 -4.79 -3.97
Cell Periphery 0.68 0.86 -0.08 -2.32 -4.37 -2.79 -0.07 -4.38 -3.72 -4.38 -1.59 -0.79 -6.26 -5.50 -6.15
Cytoplasm -0.11 1.04 -0.50 -0.39 -2.62 -0.95 2.17 -2.90 -1.02 -4.07 -1.13 1.85 -3.04 -1.34 -3.92
Cytoplasmic Foci 0.36 1.96 1.53 1.69 -1.52 -0.47 -1.00 1.39 1.49 2.05 -0.76 1.02 0.91 1.45 -0.07
Eisosomes -1.50 0.77 0.14 1.99 -0.92 -0.95 10.75 15.04 11.61 3.06 -0.33 11.70 14.58 11.43 0.51
Endoplasmic Reticulum 1.08 1.04 1.05 -3.58 0.59 -1.86 3.03 -2.91 -1.23 -3.54 0.84 0.77 0.63 -1.28 -0.60
Endosome 1.04 1.04 -0.01 -2.79 -2.80 -1.65 -1.31 -2.52 -2.09 -2.51 -0.43 0.83 -3.39 -3.27 -3.66
Golgi 1.17 1.00 0.71 -2.72 -1.69 -1.18 -10.69 -3.05 -0.99 -1.24 -0.48 -2.08 -2.81 -1.92 -2.04
Lipid Particles 1.28 1.31 1.22 -1.43 -2.17 -1.50 0.67 -2.51 1.08 -2.66 -0.63 1.34 0.12 0.79 -2.91
Mitochondria 0.69 -0.66 -3.57 -4.50 -3.81 -2.23 -1.96 -7.02 -5.22 -3.32 -1.91 -2.90 -6.57 -5.57 -4.92
Mitotic Spindle 2.13 -3.46 -0.81 -8.89 3.99 -0.00 -4.33 -5.51 -5.21 0.43 0.90 -7.60 -7.61 -7.81 3.70
None -1.11 0.16 -2.00 0.70 -4.22 -0.40 1.85 -4.61 -4.68 -7.84 -1.18 0.47 -5.07 -2.06 -7.91
Nuclear Periphery 1.12 0.60 0.04 -4.46 -2.29 -1.69 -2.26 -5.48 -4.41 -4.34 -1.04 -2.95 -8.37 -6.67 -4.51
Nuclear Periphery Foci 1.78 1.92 1.58 -1.30 -3.24 -1.60 2.25 -2.04 -0.82 -2.39 -0.81 2.46 -2.11 -0.89 -3.69
Nucleolus 0.10 -1.53 -5.39 -9.44 -7.64 -9.58 -1.90 -11.77 -3.87 -6.46 -8.69 -4.06 -17.40 -8.85 -9.90
Nucleus -1.71 -0.19 -2.52 -0.89 -4.69 -9.18 3.00 -15.43 -8.13 -14.67 -9.71 -1.10 -17.35 -6.89 -12.32
Peroxisomes 0.35 2.34 8.14 13.23 13.59 17.70* 1.79 39.56* 23.18 22.13* 16.82 8.68 41.11* 26.21 24.07*
Vacuole 1.27 1.51 -1.15 -2.75 -3.01 -3.21 -2.00 -3.17 -2.65 -3.03 -2.80 -0.66 -4.36 -3.64 -4.27
Vacuole Periphery 0.95 0.62 -3.00 -3.61 -3.48 -5.00 -5.92 -5.86 -4.70 -5.10 -4.92 -6.80 -5.93 -5.02 -5.23
Standard name
Human Ortholog
Description RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p
Localization
Cell Percentages nucleolus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rpa49

Rpa49


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpa49-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available