Standard name
Description Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments; human gene CARS allows growth of the yeast haploid null mutant after sporulation of a heterozygous diploid

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0 0 0 0 0.06 0.07 0.1 0.08 0.06 0 0.06 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.95 0.97 0.94 0.86 0.76 0.69 0.64 0.68 0.65 0.66 0.94 0.94 0.96 0.99 0.96 0.98 0.98 0.94 0.9 0.88 0.86 0.87
Endoplasmic Reticulum 0 0 0 0 0 0.09 0.12 0.16 0.1 0.14 0.14 0.08 0.06 0.05 0 0 0 0 0 0 0 0 0.05 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.14 0.11 0.25 0.17 0.25 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 6 12 11 11 15 11 16 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 2 1 1 0 3 7 5 2 3 12 0 0 1 0 0 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6 3 5 12 6 14 18 19 21 33 34 36 10 14 27 10 14 13 0 0 1 0 0 0
Cytoplasm 206 150 110 232 308 300 325 341 241 308 212 313 164 370 424 209 252 297 204 146 119 164 249 296
Endoplasmic Reticulum 9 1 2 1 11 31 52 80 36 62 47 38 11 21 8 7 8 12 1 2 0 7 14 16
Endosome 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 1 0 0 0 0 0 1 2 1 0 0 0 0 1 0 1 1 0 0 0 0 0 0 1
Mitochondria 1 0 0 2 5 15 60 52 95 78 83 117 0 0 0 1 1 0 0 1 1 3 1 2
Nucleus 0 1 0 1 3 8 3 8 4 5 1 0 1 1 3 0 0 0 0 1 1 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 0 0 7 18 7 9 7 13 0 4 1 0 7 1 0 0 4 7 15 15
Unique Cell Count 212 152 116 240 326 347 430 494 374 455 328 471 175 392 440 212 262 304 208 156 132 186 289 343
Labelled Cell Count 223 156 119 250 334 374 482 540 422 514 399 547 186 411 464 228 283 323 208 156 132 186 289 343


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.3 28.1 16.7 18.6 15.3 14.0 12.3 12.4 11.1 10.9 11.1 10.7 24.3 23.9 25.1 29.9 29.1 31.5 26.7 27.0 28.1
Std Deviation (1e-4) 3.9 5.7 3.0 3.3 2.8 2.8 2.7 2.9 2.6 2.5 2.8 2.6 4.9 5.1 4.9 7.5 6.8 5.9 5.2 6.1 6.6
Intensity Change (Log2) 0.16 -0.13 -0.26 -0.44 -0.44 -0.59 -0.62 -0.6 -0.64 0.54 0.51 0.58 0.84 0.8 0.91 0.67 0.69 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 0 0 0 0 0.5 1.1 2.0 1.3 0.5 0 0.8 0.2 0.4 0
Cytoplasm 0.8 -0.1 -2.5 -4.6 -5.7 -6.3 -5.9 -6.3 -6.1 -0.4 -0.2 0.8 2.0 0.6 1.5
Endoplasmic Reticulum 0 0 2.6 3.3 4.1 2.8 3.6 3.7 2.4 1.8 1.7 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 3.7 6.0 4.8 6.0 6.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Crs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available