Standard name | |
---|---|
Human Ortholog | |
Description | Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 17 | 2 | 1 | 2 | 1 | 1 | 12 | – | 8 | 8 | 7 | 11 | 8 | 8 | 1 | 9 | 8 | 13 | 10 | 14 | 3 | 9 | 12 |
Bud | 6 | 4 | 4 | 2 | 3 | 6 | 10 | 14 | – | 19 | 19 | 36 | 0 | 2 | 2 | 2 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 1 | 2 | 0 | 1 | 2 | 3 | – | 1 | 1 | 2 | 0 | 1 | 2 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 6 | 18 | 8 | 23 | 28 | 36 | 33 | – | 37 | 40 | 44 | 6 | 13 | 18 | 29 | 12 | 20 | 0 | 0 | 0 | 0 | 2 | 1 |
Endoplasmic Reticulum | 1 | 2 | 1 | 2 | 8 | 6 | 7 | 13 | – | 11 | 11 | 5 | 7 | 2 | 5 | 11 | 12 | 2 | 0 | 1 | 0 | 3 | 3 | 4 |
Endosome | 85 | 18 | 104 | 94 | 100 | 83 | 71 | 138 | – | 33 | 32 | 64 | 32 | 57 | 60 | 141 | 67 | 56 | 133 | 58 | 99 | 38 | 47 | 63 |
Golgi | 238 | 60 | 80 | 28 | 65 | 16 | 18 | 39 | – | 23 | 15 | 7 | 65 | 84 | 84 | 62 | 50 | 90 | 376 | 169 | 200 | 127 | 149 | 234 |
Mitochondria | 400 | 225 | 224 | 127 | 121 | 198 | 277 | 282 | – | 313 | 325 | 332 | 46 | 73 | 41 | 26 | 15 | 13 | 16 | 13 | 13 | 16 | 12 | 13 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 7 | 2 | 3 | 1 | 4 | – | 8 | 3 | 1 | 4 | 1 | 0 | 5 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 37 | 10 | 20 | 35 | 15 | 38 | 36 | 59 | – | 33 | 25 | 29 | 2 | 0 | 1 | 16 | 16 | 14 | 0 | 1 | 1 | 0 | 0 | 0 |
Peroxisomes | 9 | 4 | 39 | 6 | 5 | 12 | 7 | 17 | – | 5 | 11 | 11 | 2 | 9 | 8 | 26 | 13 | 11 | 70 | 25 | 79 | 34 | 32 | 17 |
SpindlePole | 13 | 1 | 16 | 10 | 8 | 12 | 14 | 24 | – | 12 | 10 | 25 | 4 | 9 | 24 | 61 | 36 | 16 | 4 | 3 | 3 | 2 | 0 | 1 |
Vac/Vac Membrane | 68 | 25 | 26 | 27 | 94 | 52 | 57 | 56 | – | 40 | 21 | 34 | 19 | 37 | 31 | 32 | 19 | 19 | 12 | 6 | 3 | 0 | 5 | 22 |
Unique Cell Count | 581 | 269 | 376 | 247 | 332 | 356 | 422 | 563 | 430 | 437 | 491 | 135 | 215 | 211 | 316 | 191 | 202 | 633 | 293 | 421 | 230 | 268 | 375 | |
Labelled Cell Count | 876 | 379 | 535 | 349 | 446 | 457 | 537 | 697 | 545 | 527 | 599 | 198 | 297 | 284 | 418 | 259 | 254 | 633 | 293 | 421 | 230 | 268 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.6 | 10.2 | 9.1 | 8.5 | 8.3 | 7.1 | 6.7 | 7.0 | – | 6.2 | 6.4 | 6.5 | 8.9 | 8.8 | 8.2 | 7.3 | 7.5 | 9.0 | 10.7 | 10.4 | 11.4 |
Std Deviation (1e-4) | 1.8 | 2.1 | 1.7 | 1.9 | 1.6 | 1.4 | 1.4 | 1.3 | – | 1.2 | 1.2 | 1.4 | 1.4 | 1.6 | 1.5 | 1.2 | 1.6 | 1.8 | 2.0 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.13 | -0.35 | -0.45 | -0.37 | – | -0.56 | -0.5 | -0.48 | -0.03 | -0.05 | -0.15 | -0.33 | -0.27 | -0.01 | 0.23 | 0.19 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.4 | 1.6 | – | 2.9 | 2.8 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | 1.2 | 1.7 | 2.1 | 0.7 | – | 2.1 | 2.4 | 2.4 | -0.2 | 0.7 | 1.8 | 2.3 | 0.8 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.5 | – | 2.7 | 2.7 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Endosome | 2.7 | 0.7 | -1.3 | -3.7 | -1.1 | – | -7.5 | -7.7 | -5.4 | -0.9 | -0.3 | 0.2 | 4.6 | 1.8 | 0 |
Golgi | -3.2 | -0.6 | -6.7 | -7.3 | -6.5 | – | -6.8 | -7.9 | -9.6 | 5.9 | 4.6 | 4.8 | -0.5 | 1.3 | 5.9 |
Mitochondria | -2.0 | -6.1 | -1.1 | 1.8 | -2.9 | – | 4.0 | 4.5 | 2.4 | -5.1 | -6.0 | -9.4 | -14.0 | -11.8 | -12.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.8 | -0.5 | 2.7 | 1.8 | 2.8 | – | 1.3 | 0.2 | 0.4 | -1.9 | -3.4 | -3.0 | -0.2 | 1.4 | 0.8 |
Peroxisomes | -3.7 | -4.9 | -3.7 | -5.3 | -4.7 | – | -5.7 | -4.6 | -5.1 | -3.3 | -2.6 | -2.8 | -1.0 | -1.4 | -2.0 |
SpindlePole | -0.1 | -1.4 | -0.6 | -0.7 | 0 | – | -1.1 | -1.6 | 0.6 | -0.7 | 0 | 3.3 | 6.3 | 5.7 | 1.8 |
Vacuole | 1.8 | 7.6 | 3.4 | 3.0 | 1.6 | – | 1.2 | -1.3 | 0 | 2.5 | 3.9 | 3.1 | 1.5 | 1.3 | 1.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6525 | 6.328 | 5.9084 | 6.0076 | 5.3468 | 5.9362 | 5.7678 | 7.6593 | 7.2833 | 7.2638 | 6.8235 | 7.2874 | 7.1707 | 8.7002 | 8.1933 | 7.6902 | 7.7615 | 8.1687 |
Actin | 0.0102 | 0.0096 | 0.005 | 0.006 | 0.0072 | 0.0137 | 0.0044 | 0.0046 | 0.0278 | 0.0021 | 0.0017 | 0.0048 | 0.0113 | 0.0152 | 0.0176 | 0.0236 | 0.0108 | 0.0116 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0004 | 0.0002 | 0.0054 | 0.0003 | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 |
Bud Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0003 | 0.0008 | 0.0003 | 0.0045 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0011 | 0.0014 | 0.0008 | 0.0009 | 0.0007 | 0.0006 | 0.0007 | 0.0006 | 0.0006 | 0.0003 | 0.0008 | 0.0058 | 0.0005 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0004 |
Cytoplasmic Foci | 0.1173 | 0.16 | 0.1124 | 0.1012 | 0.1009 | 0.0984 | 0.1598 | 0.1415 | 0.0978 | 0.0964 | 0.088 | 0.0802 | 0.1346 | 0.1353 | 0.1314 | 0.1332 | 0.1218 | 0.1052 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0005 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0006 |
Endosome | 0.1891 | 0.2235 | 0.2029 | 0.2262 | 0.2221 | 0.1294 | 0.1376 | 0.1468 | 0.1741 | 0.1515 | 0.1632 | 0.1022 | 0.146 | 0.1652 | 0.1191 | 0.1399 | 0.1042 | 0.0949 |
Golgi | 0.4469 | 0.4844 | 0.5568 | 0.5205 | 0.5576 | 0.5982 | 0.3207 | 0.5222 | 0.5504 | 0.5984 | 0.6322 | 0.5251 | 0.466 | 0.5576 | 0.5508 | 0.5755 | 0.5329 | 0.5933 |
Lipid Particles | 0.0882 | 0.0384 | 0.0404 | 0.0326 | 0.0414 | 0.0485 | 0.1438 | 0.0998 | 0.0443 | 0.0476 | 0.069 | 0.1389 | 0.0686 | 0.0322 | 0.0466 | 0.0238 | 0.0678 | 0.0652 |
Mitochondria | 0.0358 | 0.0168 | 0.025 | 0.0242 | 0.0069 | 0.0213 | 0.0075 | 0.013 | 0.0176 | 0.0163 | 0.0107 | 0.0225 | 0.0152 | 0.0126 | 0.0159 | 0.005 | 0.004 | 0.0144 |
None | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0011 |
Nuclear Periphery | 0.0016 | 0.0001 | 0.0003 | 0.0012 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0004 |
Nucleolus | 0.0002 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Nucleus | 0.0005 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Peroxisomes | 0.1026 | 0.0589 | 0.0518 | 0.063 | 0.0562 | 0.0861 | 0.1891 | 0.0684 | 0.0828 | 0.0849 | 0.0297 | 0.1169 | 0.1551 | 0.0769 | 0.1164 | 0.0973 | 0.1566 | 0.1103 |
Punctate Nuclear | 0.0014 | 0.0006 | 0.0004 | 0.0035 | 0.0014 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Vacuole | 0.0013 | 0.0023 | 0.0014 | 0.003 | 0.0017 | 0.001 | 0.0273 | 0.0006 | 0.0012 | 0.0006 | 0.0008 | 0.0006 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0005 |
Vacuole Periphery | 0.0018 | 0.0017 | 0.0016 | 0.0035 | 0.0021 | 0.0012 | 0.0063 | 0.0011 | 0.002 | 0.0011 | 0.001 | 0.0015 | 0.0007 | 0.0007 | 0.0006 | 0.0006 | 0.0005 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.816 | 33.4843 | 26.9423 | 22.5427 | 28.9633 | 21.6499 | 34.3659 | 28.3567 | 25.4363 | 34.5977 |
Translational Efficiency | 0.7544 | 0.6508 | 0.514 | 0.5839 | 0.6382 | 0.8113 | 0.6084 | 0.5105 | 0.4613 | 0.4985 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
216 | 1386 | 276 | 147 | 179 | 1657 | 296 | 1293 | 395 | 3043 | 572 | 1440 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 997.17 | 1019.54 | 1275.71 | 1131.95 | 943.60 | 990.27 | 1072.71 | 1012.75 | 972.89 | 1003.60 | 1170.66 | 1024.92 |
Standard Deviation | 137.08 | 159.12 | 141.20 | 144.84 | 121.22 | 142.37 | 113.68 | 134.39 | 132.84 | 150.94 | 163.08 | 140.21 |
Intensity Change Log 2 | — | 0.032007 | 0.355389 | 0.182899 | — | 0.069647 | 0.185013 | 0.102031 | — | 0.050430 | 0.275061 | 0.144147 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003848 | 0.009907 | 0.016554 | 0.020466 | 0.003186 | 0.007226 | 0.022114 | 0.008792 | 0.003548 | 0.008448 | 0.019431 | 0.009984 |
Bud Neck | 0.001552 | 0.000404 | 0.002071 | 0.001687 | 0.000308 | 0.000509 | 0.000787 | 0.000617 | 0.000988 | 0.000461 | 0.001406 | 0.000726 |
Bud Site | 0.017776 | 0.000919 | 0.004165 | 0.015574 | 0.000543 | 0.000680 | 0.002179 | 0.007561 | 0.009967 | 0.000789 | 0.003137 | 0.008379 |
Cell Periphery | 0.000202 | 0.000068 | 0.000086 | 0.000101 | 0.000137 | 0.000064 | 0.000075 | 0.000051 | 0.000172 | 0.000066 | 0.000080 | 0.000056 |
Cytoplasm | 0.017974 | 0.000818 | 0.000769 | 0.003547 | 0.000735 | 0.001053 | 0.000739 | 0.005248 | 0.010162 | 0.000946 | 0.000753 | 0.005075 |
Cytoplasmic Foci | 0.238444* | 0.306722* | 0.312044* | 0.316821* | 0.282233* | 0.284406* | 0.364595* | 0.281391* | 0.258288* | 0.294571* | 0.339238* | 0.285008* |
Eisosomes | 0.000071 | 0.000119 | 0.000089 | 0.000070 | 0.000062 | 0.000090 | 0.000088 | 0.000046 | 0.000067 | 0.000104 | 0.000088 | 0.000049 |
Endoplasmic Reticulum | 0.003518 | 0.000282 | 0.000961 | 0.000567 | 0.000248 | 0.000246 | 0.000608 | 0.000272 | 0.002036 | 0.000262 | 0.000779 | 0.000303 |
Endosome | 0.349625* | 0.146096 | 0.140056 | 0.265300* | 0.263787* | 0.251588* | 0.215775* | 0.291921* | 0.310726* | 0.203539* | 0.179239 | 0.289203* |
Golgi | 0.162645* | 0.421360* | 0.445163* | 0.237998* | 0.336188* | 0.304322* | 0.295951* | 0.326192* | 0.241288* | 0.357629* | 0.367948* | 0.317189* |
Lipid Particles | 0.007756 | 0.038250 | 0.033153 | 0.006311 | 0.039156 | 0.051168 | 0.035946 | 0.005523 | 0.021985 | 0.045284 | 0.034598 | 0.005603 |
Mitochondria | 0.010217 | 0.039933 | 0.003622 | 0.001995 | 0.030925 | 0.052227 | 0.003670 | 0.007971 | 0.019601 | 0.046627 | 0.003647 | 0.007361 |
Mitotic Spindle | 0.018874 | 0.003098 | 0.005668 | 0.030897 | 0.002251 | 0.001374 | 0.011168 | 0.015439 | 0.011341 | 0.002159 | 0.008514 | 0.017017 |
None | 0.000033 | 0.000063 | 0.000030 | 0.000007 | 0.000057 | 0.000048 | 0.000015 | 0.000036 | 0.000044 | 0.000055 | 0.000022 | 0.000033 |
Nuclear Periphery | 0.000228 | 0.000074 | 0.000503 | 0.000054 | 0.000158 | 0.000087 | 0.000265 | 0.000085 | 0.000197 | 0.000081 | 0.000380 | 0.000082 |
Nuclear Periphery Foci | 0.000893 | 0.000352 | 0.001239 | 0.000571 | 0.000217 | 0.000727 | 0.001756 | 0.000883 | 0.000587 | 0.000556 | 0.001507 | 0.000851 |
Nucleolus | 0.000059 | 0.000130 | 0.000039 | 0.000022 | 0.001519 | 0.000071 | 0.000013 | 0.000206 | 0.000721 | 0.000098 | 0.000025 | 0.000187 |
Nucleus | 0.001084 | 0.000283 | 0.000045 | 0.000050 | 0.002782 | 0.000038 | 0.000022 | 0.000063 | 0.001853 | 0.000150 | 0.000033 | 0.000061 |
Peroxisomes | 0.025535 | 0.026567 | 0.010421 | 0.061833 | 0.025310 | 0.035399 | 0.030571 | 0.026655 | 0.025433 | 0.031376 | 0.020848 | 0.030246 |
Vacuole | 0.122195 | 0.002602 | 0.017309 | 0.032944 | 0.005133 | 0.005150 | 0.010112 | 0.015950 | 0.069147 | 0.003989 | 0.013585 | 0.017685 |
Vacuole Periphery | 0.017473 | 0.001952 | 0.006014 | 0.003184 | 0.005066 | 0.003528 | 0.003553 | 0.005097 | 0.011851 | 0.002811 | 0.004740 | 0.004901 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.89 | -3.90 | -2.51 | -1.61 | -0.58 | -3.06 | -3.85 | -3.63 | -0.90 | 2.63 | -4.70 | -5.30 | -4.14 | -0.98 | 2.90 |
Bud Neck | 5.01 | -1.11 | -0.33 | -3.22 | 0.63 | -1.44 | -3.85 | -2.54 | -0.74 | 1.08 | 3.60 | -1.71 | 1.55 | -2.21 | 2.95 |
Bud Site | 3.36 | 2.65 | 0.41 | -4.77 | -3.47 | -1.01 | -5.15 | -7.77 | -7.63 | -5.75 | 3.31 | 2.43 | 0.62 | -8.71 | -4.98 |
Cell Periphery | 3.06 | 2.64 | 2.05 | -1.50 | -0.71 | 1.37 | 1.15 | 1.60 | 2.13 | 2.69 | 3.13 | 2.69 | 3.40 | 1.74 | 3.62 |
Cytoplasm | 3.64 | 3.64 | 3.09 | -3.74 | -3.55 | -0.65 | -0.01 | -4.27 | -3.93 | -4.43 | 3.53 | 3.59 | 1.90 | -4.41 | -4.63 |
Cytoplasmic Foci | -5.11 | -4.40 | -3.82 | -0.50 | -0.17 | -0.16 | -4.87 | 0.08 | 0.48 | 7.09 | -3.76 | -6.76 | -2.57 | 1.70 | 5.95 |
Eisosomes | -2.80 | -0.98 | -0.03 | 3.21 | 1.07 | -3.30 | -2.08 | 1.91 | 8.56 | 4.05 | -3.62 | -1.85 | 1.76 | 11.54 | 5.35 |
Endoplasmic Reticulum | 3.18 | 2.50 | 2.85 | -1.95 | 2.09 | 0.02 | -3.52 | -0.47 | -0.29 | 3.29 | 3.14 | 2.21 | 3.07 | -0.67 | 5.95 |
Endosome | 11.32 | 10.96 | 3.49 | -6.98 | -6.86 | 0.73 | 2.62 | -1.63 | -5.10 | -7.04 | 8.43 | 9.62 | 1.55 | -12.97 | -13.22 |
Golgi | -19.70 | -15.14 | -3.71 | 10.72 | 9.56 | 1.96 | 2.07 | 0.65 | -2.60 | -2.19 | -10.45 | -8.86 | -6.30 | 5.78 | 4.47 |
Lipid Particles | -7.44 | -4.83 | 0.21 | 8.91 | 5.44 | -1.14 | 0.28 | 3.33 | 14.38 | 5.21 | -4.37 | -2.07 | 3.27 | 17.83 | 7.79 |
Mitochondria | -5.72 | 1.60 | 2.02 | 10.62 | 1.10 | -2.80 | 4.00 | 3.34 | 12.18 | -3.06 | -6.02 | 4.15 | 3.11 | 14.75 | -2.97 |
Mitotic Spindle | 2.37 | 1.86 | -1.14 | -3.12 | -2.75 | 1.05 | -2.35 | -6.51 | -7.51 | -1.08 | 2.50 | 0.62 | -1.43 | -7.58 | -2.98 |
None | -1.47 | 0.24 | 1.79 | 4.02 | 6.18 | 0.25 | 1.34 | 0.53 | 0.46 | -0.98 | -0.58 | 1.35 | 0.42 | 0.98 | -0.57 |
Nuclear Periphery | 2.55 | -2.59 | 2.89 | 1.33 | 5.07 | 0.91 | -0.82 | 0.91 | 0.06 | 1.73 | 2.38 | -2.19 | 2.30 | -0.12 | 4.31 |
Nuclear Periphery Foci | 1.65 | -0.93 | 0.78 | -1.40 | 2.31 | -1.69 | -1.96 | -2.35 | -0.42 | 1.02 | 0.11 | -2.04 | -0.93 | -1.02 | 1.29 |
Nucleolus | -0.80 | 0.62 | 1.45 | 1.25 | 0.72 | 0.97 | 1.00 | 0.86 | -0.72 | -1.02 | 0.91 | 1.02 | 0.75 | -0.51 | -0.95 |
Nucleus | 1.04 | 1.42 | 1.42 | 1.02 | -0.30 | 1.00 | 1.01 | 0.99 | -0.67 | -1.08 | 1.30 | 1.40 | 1.38 | 0.78 | -0.85 |
Peroxisomes | -0.19 | 2.70 | -3.30 | -3.54 | -5.12 | -1.96 | -0.75 | -0.26 | 2.62 | 0.67 | -1.54 | 1.00 | -1.15 | 0.33 | -2.50 |
Vacuole | 7.84 | 6.78 | 5.05 | -3.65 | -1.85 | -0.01 | -2.09 | -6.00 | -6.90 | -2.45 | 7.36 | 6.18 | 5.70 | -8.68 | -1.94 |
Vacuole Periphery | 4.09 | 2.94 | 3.72 | -1.84 | 2.75 | 1.29 | 1.04 | -0.02 | -1.90 | -1.32 | 4.17 | 3.16 | 3.11 | -3.20 | -0.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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