ORF
Human Ortholog
Description Protein of unknown function with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.99 0.97 0.95 0.84 0.74 0.8 0.72 0.7 0.6 0.68 0.99 0.99 0.99 – – 1.0 0.87 0.95 0.89 0.95 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.08 0.33 0.49 0.34 0.46 0.51 0.62 0.49 0 0 0 – – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 0 1 1 0 0 0 – – 0 2 0 4 0 5 4
Bud 1 1 2 2 5 10 21 15 26 34 26 29 0 0 0 – – 0 0 0 2 1 1 9
Bud Neck 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 – – 0 0 0 1 0 1 4
Bud Site 0 0 0 0 0 0 1 1 1 7 4 4 0 0 0 – – 0 – – – – – –
Cell Periphery 5 0 8 8 2 6 9 10 15 11 13 23 1 4 0 – – 1 0 0 2 0 0 2
Cytoplasm 232 191 387 335 300 452 550 583 423 525 365 436 429 540 568 – – 48 235 196 344 293 361 390
Endoplasmic Reticulum 0 3 0 0 0 2 3 1 0 0 2 0 1 2 2 – – 1 7 0 3 0 0 2
Endosome 3 1 1 2 1 2 1 5 5 0 1 2 1 2 0 – – 0 3 2 1 1 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 0 2
Mitochondria 5 5 3 24 25 175 366 248 269 377 377 316 0 1 0 – – 0 7 1 17 0 5 6
Nucleus 0 1 0 0 0 2 4 4 3 1 2 0 0 0 3 – – 0 0 0 0 3 1 0
Nuclear Periphery 1 0 0 0 1 1 2 2 2 0 2 2 0 0 0 – – 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 – – 0 0 0 2 0 0 0
Peroxisomes 3 0 0 0 0 0 0 0 1 0 1 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 1 8 3 4 3 2 1 0 0 – – 0 2 0 0 0 3 5
Vac/Vac Membrane 1 0 2 1 2 9 3 15 12 12 16 12 0 0 1 – – 0 4 0 1 3 3 3
Unique Cell Count 242 197 390 344 316 537 744 728 590 745 610 640 432 544 571 48 270 208 387 310 400 446
Labelled Cell Count 251 202 403 373 336 663 961 893 762 971 813 828 433 549 574 50 270 208 387 310 400 446


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.9 4.9 4.5 4.5 4.0 3.8 3.9 3.7 3.6 3.4 3.5 6.3 6.1 5.8 – – 6.5 6.2 6.3 6.5
Std Deviation (1e-4) 0.6 0.7 0.9 1.9 1.3 1.7 1.4 1.3 1.0 1.0 1.0 1.0 1.1 0.9 1.0 – – 1.3 1.2 1.2 1.5
Intensity Change (Log2) – – – -0.11 -0.14 -0.29 -0.36 -0.34 -0.39 -0.46 -0.51 -0.47 0.35 0.32 0.25 – – 0.42 0.34 0.36 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 -1.8 0 – – 0
Cytoplasm -2.0 -3.5 -7.7 -10.7 -9.0 -11.1 -11.6 -14.1 -12.1 0.1 0.1 0.5 – – 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Vacuole 0 0 0 0 2.0 2.0 0 2.4 1.8 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5522 2.6718 2.9694 2.8737 2.7695 3.121 0.6008 0.788 0.7029 0.4468 0.6007 0.4415 -0.4744 0.1528 -0.1985 -0.0892 -0.2163 -0.1102
Actin 0.0106 0.0121 0.0059 0.0004 0.0023 0.0013 0.0405 0.0056 0.0152 0.0326 0.0278 0.0024 0.0115 0.0007 0.0038 0.0041 0.0571 0.0041
Bud 0.0015 0.0004 0.0002 0.0002 0.0001 0.0003 0.0083 0.004 0.0037 0.0028 0.0007 0.0006 0.002 0.0161 0.0099 0.0114 0.0096 0.0022
Bud Neck 0.0063 0.0002 0.0007 0.0001 0.0002 0.0047 0.0013 0.0003 0.0009 0.0007 0.0008 0.0006 0.0029 0.0006 0.0006 0.0008 0.0025 0.0018
Bud Periphery 0.0065 0.0008 0.0004 0.0001 0.0001 0.0005 0.004 0.0053 0.0063 0.0151 0.0011 0.0008 0.0028 0.0027 0.0019 0.0054 0.0159 0.0015
Bud Site 0.003 0.0031 0.002 0.0002 0.0001 0.0001 0.0053 0.0167 0.009 0.0024 0.0012 0.0002 0.0015 0.0226 0.0079 0.0014 0.0028 0.0004
Cell Periphery 0.0012 0.0004 0.0004 0.0001 0.0001 0.0001 0.0003 0.0004 0.0004 0.0003 0.0002 0 0.0002 0.0003 0.0003 0.0001 0.0003 0.0001
Cytoplasm 0.3074 0.5589 0.3968 0.3777 0.4232 0.525 0.5329 0.7273 0.6341 0.6456 0.5884 0.8255 0.5099 0.589 0.6009 0.5371 0.4877 0.735
Cytoplasmic Foci 0.0168 0.0062 0.011 0.0158 0.0184 0.0104 0.0318 0.0078 0.021 0.0216 0.0185 0.007 0.0184 0.0094 0.0182 0.025 0.0227 0.0203
Eisosomes 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0 0.0003 0.0001 0.0001 0.0001 0.0006 0
Endoplasmic Reticulum 0.0024 0.002 0.0022 0.0014 0.001 0.0018 0.0053 0.0034 0.0047 0.0039 0.006 0.0019 0.0026 0.0019 0.003 0.0055 0.0099 0.0017
Endosome 0.0212 0.0009 0.0056 0.0069 0.0064 0.0034 0.0403 0.0033 0.0185 0.0084 0.0225 0.0049 0.0079 0.0014 0.0051 0.0206 0.0365 0.006
Golgi 0.0088 0.0003 0.0028 0.0015 0.0011 0.0003 0.0147 0.0024 0.0067 0.0089 0.0337 0.0042 0.0026 0.0002 0.0011 0.0024 0.0078 0.0006
Lipid Particles 0.0083 0.0002 0.0023 0.0001 0.003 0.0002 0.0114 0.0023 0.0025 0.0038 0.0146 0.0021 0.0081 0.0002 0.0023 0.0035 0.0036 0.0007
Mitochondria 0.0048 0.0003 0.0034 0.0009 0.0002 0.0004 0.0111 0.0011 0.0112 0.0034 0.0382 0.0037 0.0012 0.0002 0.001 0.0009 0.0041 0.0006
None 0.5795 0.4109 0.5412 0.5914 0.5319 0.449 0.2321 0.2142 0.2416 0.2272 0.2332 0.1416 0.4146 0.3464 0.334 0.3602 0.2796 0.213
Nuclear Periphery 0.001 0.0003 0.0005 0.0004 0.0007 0.0003 0.017 0.0009 0.003 0.0021 0.0012 0.0006 0.0014 0.0005 0.001 0.0032 0.0266 0.0011
Nucleolus 0.0035 0.0001 0.0005 0.0001 0.0001 0.0001 0.0103 0.0001 0.0051 0.0002 0.0002 0 0.0006 0.0003 0.0003 0.0007 0.0016 0.0003
Nucleus 0.0028 0.001 0.0009 0.0007 0.0005 0.0007 0.0106 0.0015 0.0028 0.0025 0.0011 0.0016 0.0019 0.0026 0.0023 0.005 0.013 0.0033
Peroxisomes 0.0062 0.0004 0.0179 0.001 0.0035 0.0004 0.0102 0.0008 0.0078 0.0131 0.0061 0.0006 0.0042 0.0003 0.0027 0.0017 0.0017 0.0014
Punctate Nuclear 0.0007 0.0005 0.0022 0.0003 0.0067 0.0006 0.0053 0.0012 0.0019 0.0023 0.0006 0.0007 0.0043 0.0036 0.0024 0.0088 0.0081 0.0046
Vacuole 0.0064 0.0009 0.0026 0.0006 0.0005 0.0004 0.0042 0.0012 0.0026 0.0024 0.0025 0.0008 0.001 0.0009 0.0013 0.0016 0.0056 0.001
Vacuole Periphery 0.0007 0.0001 0.0004 0.0001 0.0001 0.0001 0.003 0.0002 0.0008 0.0006 0.0012 0.0002 0.0002 0.0001 0.0002 0.0004 0.0025 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4602 2.31 2.2855 3.7919 5.584 4.3044 7.9801 4.4665 4.5258 6.5486
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1972 862 1547 106 1814 2259 534 963 3786 3121 2081 1069

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 704.81 746.70 879.53 963.11 687.85 768.92 937.59 978.45 696.68 762.78 894.43 976.93
Standard Deviation 76.82 97.54 104.71 123.87 89.68 91.64 100.76 135.82 83.66 93.83 106.76 134.76
Intensity Change Log 2 — 0.083294 0.319498 0.450466 — 0.160740 0.446863 0.508404 — 0.122065 0.383811 0.479373

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000289 0.000463 0.000223 0.000392 0.000101 0.000137 0.000103 0.002220 0.000199 0.000227 0.000192 0.002038
Bud Neck 0.018875 0.021361 0.002459 0.001861 0.007619 0.023633 0.003428 0.004644 0.013482 0.023005 0.002708 0.004368
Bud Site 0.006924 0.021926 0.004591 0.004513 0.003501 0.012767 0.004320 0.042174 0.005284 0.015296 0.004522 0.038439
Cell Periphery 0.000142 0.000148 0.000137 0.000334 0.000103 0.000159 0.000194 0.000182 0.000123 0.000156 0.000152 0.000197
Cytoplasm 0.505502* 0.487099* 0.548234* 0.395432* 0.598191* 0.479548* 0.435512* 0.581573* 0.549912* 0.481634* 0.519309* 0.563115*
Cytoplasmic Foci 0.257664* 0.255664* 0.018192 0.076627 0.173812 0.153186 0.059735 0.037050 0.217488* 0.181490* 0.028852 0.040975
Eisosomes 0.000273 0.000107 0.000037 0.000135 0.000106 0.000118 0.000104 0.000045 0.000193 0.000115 0.000054 0.000054
Endoplasmic Reticulum 0.001254 0.001388 0.002552 0.004611 0.001230 0.001146 0.001513 0.001264 0.001243 0.001213 0.002286 0.001596
Endosome 0.014798 0.026126 0.003444 0.008858 0.007522 0.018585 0.006160 0.009497 0.011312 0.020668 0.004141 0.009434
Golgi 0.005011 0.004663 0.000107 0.000153 0.001805 0.002579 0.000080 0.006383 0.003475 0.003155 0.000100 0.005766
Lipid Particles 0.008177 0.004404 0.001519 0.004425 0.005295 0.007737 0.003134 0.000844 0.006796 0.006816 0.001933 0.001199
Mitochondria 0.004139 0.005547 0.001009 0.000147 0.002029 0.005381 0.000354 0.005230 0.003128 0.005427 0.000841 0.004726
Mitotic Spindle 0.001152 0.005328 0.003748 0.000255 0.000598 0.005389 0.000726 0.025487 0.000886 0.005372 0.002972 0.022985
None 0.001800 0.002423 0.004384 0.001634 0.002741 0.002381 0.003190 0.002711 0.002251 0.002393 0.004078 0.002604
Nuclear Periphery 0.000338 0.000353 0.001400 0.001203 0.000323 0.000567 0.001155 0.000524 0.000331 0.000508 0.001337 0.000592
Nuclear Periphery Foci 0.000533 0.000695 0.001560 0.005373 0.000537 0.000340 0.001300 0.001845 0.000535 0.000438 0.001493 0.002195
Nucleolus 0.000825 0.001216 0.000305 0.000448 0.000599 0.001744 0.000886 0.000100 0.000717 0.001598 0.000454 0.000134
Nucleus 0.102635 0.058977 0.332324* 0.155864* 0.137585* 0.149553* 0.338760* 0.093169 0.119381 0.124537 0.333976* 0.099385
Peroxisomes 0.006272 0.008957 0.000487 0.000616 0.001476 0.004516 0.000542 0.002493 0.003974 0.005743 0.000501 0.002307
Vacuole 0.062373 0.091021 0.072989 0.336882* 0.054090 0.128546 0.138577 0.180655 0.058404 0.118182 0.089819 0.196146
Vacuole Periphery 0.001024 0.002136 0.000297 0.000237 0.000737 0.001987 0.000227 0.001909 0.000887 0.002028 0.000279 0.001744

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.99 0.10 -1.54 0.62 -1.62 -1.99 -3.06 -3.53 -3.34 -3.32 -0.92 -1.75 -3.17 -3.01 -2.90
Bud Neck -0.89 13.38 14.13 8.94 2.77 -11.68 5.06 6.75 15.16 0.16 -7.39 14.85 13.41 17.04 0.24
Bud Site -6.70 2.63 3.16 7.93 0.29 -6.96 -0.28 -5.43 -1.76 -4.46 -8.56 1.12 -4.70 -0.54 -4.92
Cell Periphery -0.14 -0.24 -2.36 -1.76 -2.31 -5.11 -8.04 -0.68 2.46 4.25 -2.59 -4.92 -0.17 1.59 2.00
Cytoplasm 1.88 2.04 7.19 6.08 6.38 14.63 16.53 13.79 2.90 -4.01 10.63 11.90 11.78 4.52 2.49
Cytoplasmic Foci 0.26 49.68 26.70 21.27 -4.32 3.90 24.25 35.15 34.35 7.38 7.77 56.45 54.72 42.47 -0.62
Eisosomes 6.44 8.97 5.94 -0.58 -6.96 -1.61 -0.34 8.86 14.43 10.52 5.60 9.56 10.05 14.59 2.27
Endoplasmic Reticulum -0.20 -10.63 -4.76 -3.69 -1.50 0.45 -5.19 -2.32 -3.05 3.94 0.19 -12.00 -4.66 -3.43 7.55
Endosome -5.29 11.06 7.18 9.83 -2.93 -10.24 2.01 4.13 12.86 2.77 -9.09 11.34 8.49 15.74 0.29
Golgi 0.60 12.46 12.45 7.75 -0.20 -1.51 8.32 0.11 0.96 -2.18 0.81 14.71 2.68 2.03 -2.16
Lipid Particles 5.14 12.08 7.41 1.50 -3.85 -2.93 3.50 7.68 10.45 8.83 0.00 11.91 14.41 11.27 6.51
Mitochondria -0.92 4.63 6.05 4.35 2.72 -4.17 4.09 0.52 3.67 -1.59 -3.13 5.47 2.14 4.31 -0.98
Mitotic Spindle -2.38 -2.89 0.88 2.82 3.73 -5.07 -0.40 -5.82 -3.61 -5.70 -5.27 -3.22 -5.67 -3.47 -4.32
None -0.90 -3.91 1.75 2.14 5.60 0.84 -0.34 0.52 -0.33 0.79 -0.40 -3.46 -0.39 0.01 2.84
Nuclear Periphery -0.11 -16.71 -7.06 -6.67 2.00 -3.65 -14.85 -11.08 -8.03 5.38 -2.92 -22.97 -12.52 -10.22 9.74
Nuclear Periphery Foci -0.80 -5.59 -6.68 -6.70 -4.78 1.52 -6.15 -10.61 -12.77 -2.51 0.70 -8.38 -11.29 -14.10 -4.16
Nucleolus -1.15 1.96 2.08 3.84 0.72 -3.95 -1.37 8.54 5.67 6.52 -3.44 2.10 4.06 6.72 8.95
Nucleus 10.76 -35.44 -2.82 -5.97 14.60 -2.38 -18.48 3.41 5.22 19.31 -1.40 -38.80 -0.22 0.72 32.41
Peroxisomes -2.47 9.75 10.03 9.47 0.60 -7.89 6.90 1.93 7.51 -0.94 -3.78 10.85 7.39 10.49 -0.95
Vacuole -6.74 -9.63 -14.13 -12.86 -12.46 -18.21 -14.01 -30.96 -22.23 -12.65 -18.96 -16.36 -33.48 -25.87 -24.05
Vacuole Periphery -3.36 8.74 12.68 6.23 1.81 -7.38 7.80 0.55 3.98 -1.09 -7.52 10.22 1.26 4.63 -0.92
ORF
Human Ortholog
Description Protein of unknown function with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YNL234W

YNL234W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl234w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available