Standard name | |
---|---|
Human Ortholog | |
Description | Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 31 | 33 | 25 | 20 | 27 | 29 | 37 | 34 | 47 | 45 | 18 | 19 | 6 | 2 | 2 | 5 | 3 | 13 | 14 | 41 | 36 | 44 | 57 | 52 |
Bud | 13 | 12 | 15 | 2 | 8 | 7 | 11 | 7 | 11 | 17 | 6 | 8 | 10 | 2 | 2 | 3 | 0 | 3 | 2 | 4 | 2 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 10 | 5 | 3 | 3 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 42 | 50 | 27 | 65 | 94 | 75 | 79 | 43 | 51 | 39 | 17 | 20 | 39 | 41 | 44 | 5 | 4 | 3 | 27 | 8 | 9 | 18 | 11 | 11 |
Cytoplasm | 39 | 83 | 38 | 30 | 42 | 60 | 91 | 83 | 85 | 95 | 61 | 55 | 54 | 85 | 65 | 37 | 16 | 30 | 14 | 57 | 21 | 11 | 28 | 41 |
Endoplasmic Reticulum | 47 | 118 | 74 | 81 | 84 | 100 | 123 | 112 | 110 | 90 | 45 | 40 | 45 | 55 | 83 | 13 | 6 | 10 | 105 | 153 | 100 | 66 | 81 | 114 |
Endosome | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 1 | 1 | 2 | 18 | 16 | 5 | 16 | 20 |
Golgi | 4 | 5 | 31 | 7 | 35 | 16 | 23 | 24 | 15 | 18 | 2 | 9 | 6 | 4 | 17 | 153 | 49 | 77 | 1 | 5 | 10 | 1 | 3 | 4 |
Mitochondria | 1 | 1 | 0 | 0 | 0 | 2 | 11 | 3 | 10 | 11 | 6 | 10 | 0 | 0 | 0 | 1 | 1 | 5 | 2 | 0 | 3 | 1 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 2 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 3 | 2 | 1 | 1 | 2 | 2 | 5 | 8 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 10 | 9 | 10 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 4 | 4 |
SpindlePole | 3 | 3 | 3 | 2 | 6 | 7 | 3 | 2 | 1 | 0 | 0 | 0 | 4 | 0 | 5 | 12 | 4 | 3 | 0 | 3 | 6 | 2 | 6 | 7 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 3 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 3 | 1 | 3 | 2 | 4 | 0 | 0 | 2 | 1 |
Unique Cell Count | 134 | 242 | 168 | 156 | 220 | 227 | 290 | 245 | 245 | 245 | 132 | 123 | 131 | 144 | 171 | 210 | 82 | 140 | 182 | 317 | 217 | 162 | 224 | 274 |
Labelled Cell Count | 184 | 307 | 215 | 212 | 300 | 303 | 387 | 322 | 335 | 320 | 161 | 168 | 165 | 189 | 219 | 242 | 94 | 158 | 182 | 317 | 217 | 162 | 224 | 274 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.0 | 34.2 | 25.4 | 23.9 | 23.4 | 18.7 | 17.2 | 16.8 | 15.4 | 15.5 | 13.6 | 14.1 | 31.6 | 36.5 | 37.7 | 39.4 | 41.0 | 39.6 | 27.1 | 26.3 | 27.0 |
Std Deviation (1e-4) | 4.9 | 6.1 | 4.7 | 4.9 | 4.1 | 3.7 | 3.7 | 3.4 | 3.1 | 3.2 | 3.2 | 2.7 | 6.6 | 7.8 | 8.6 | 11.3 | 10.8 | 10.0 | 5.3 | 5.3 | 5.3 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.11 | -0.44 | -0.56 | -0.6 | -0.72 | -0.71 | -0.9 | -0.85 | 0.32 | 0.52 | 0.57 | 0.63 | 0.69 | 0.64 | 0.1 | 0.05 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.5 | -0.7 | -0.6 | -0.6 | -0.3 | 1.1 | 0.9 | -0.3 | 0.1 | -2.9 | -4.2 | -4.7 | -4.5 | -2.6 | -1.5 |
Bud | -3.1 | -2.2 | -2.5 | -2.3 | -2.7 | -1.8 | -0.7 | -1.5 | -0.8 | -0.4 | -2.9 | -3.3 | -3.4 | 0 | -2.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 5.1 | 5.6 | 3.8 | 2.7 | 0.4 | 1.2 | 0 | -0.8 | 0 | 2.8 | 2.6 | 2.2 | -4.8 | -2.5 | -4.1 |
Cytoplasm | -0.7 | -0.9 | 0.9 | 2.0 | 2.5 | 2.6 | 3.5 | 4.3 | 4.0 | 3.5 | 6.6 | 3.1 | -1.2 | -0.6 | -0.3 |
Endoplasmic Reticulum | 1.4 | -1.2 | 0 | -0.3 | 0.3 | 0.2 | -1.5 | -1.7 | -2.0 | -1.7 | -1.0 | 0.8 | -8.7 | -5.8 | -7.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -3.9 | -0.7 | -3.5 | -3.4 | -2.5 | -3.9 | -3.4 | -4.7 | -2.7 | -3.6 | -4.4 | -2.2 | 10.5 | 6.6 | 6.7 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 30.1777 | 35.7517 | 33.8499 | 27.5551 | 33.8477 | 31.6059 | 30.2707 | 35.1718 | 34.2428 | 33.9203 | 30.2747 | 32.8738 | 28.7938 | 34.7393 | 32.5773 | 26.4513 | 30.1354 | 31.9264 |
Actin | 0.011 | 0.0013 | 0.0152 | 0.0186 | 0.0161 | 0.0122 | 0.0059 | 0.0016 | 0.0025 | 0.0013 | 0.0214 | 0.0034 | 0.012 | 0.0042 | 0.0021 | 0.0359 | 0.0226 | 0.0044 |
Bud | 0.0001 | 0.0003 | 0.0007 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0006 | 0.0012 | 0.0002 | 0 | 0.0005 | 0.0002 | 0.0002 | 0.0009 | 0.0002 | 0.0001 |
Bud Neck | 0.0009 | 0.0013 | 0.011 | 0.0088 | 0.0095 | 0.0059 | 0.0009 | 0.0011 | 0.0019 | 0.0034 | 0.0008 | 0.0004 | 0.0015 | 0.0008 | 0.0024 | 0.0025 | 0.0026 | 0.0012 |
Bud Periphery | 0.0003 | 0.0003 | 0.0011 | 0.0028 | 0.0019 | 0.0009 | 0.0019 | 0.0003 | 0.002 | 0.0028 | 0.0006 | 0.0002 | 0.0015 | 0.0006 | 0.0009 | 0.0104 | 0.001 | 0.0003 |
Bud Site | 0.0008 | 0.0099 | 0.0058 | 0.001 | 0.0006 | 0.0004 | 0.0024 | 0.0046 | 0.0014 | 0.0138 | 0.0009 | 0.0001 | 0.0038 | 0.003 | 0.0007 | 0.0005 | 0.0009 | 0.0002 |
Cell Periphery | 0.1023 | 0.0563 | 0.0617 | 0.1056 | 0.0816 | 0.0649 | 0.0678 | 0.047 | 0.061 | 0.0979 | 0.0495 | 0.0305 | 0.0758 | 0.0411 | 0.0892 | 0.1029 | 0.1106 | 0.0403 |
Cytoplasm | 0.0801 | 0.0671 | 0.0982 | 0.0387 | 0.1215 | 0.0753 | 0.0582 | 0.0195 | 0.0695 | 0.0075 | 0.0532 | 0.0142 | 0.0347 | 0.0337 | 0.0458 | 0.0358 | 0.0558 | 0.0221 |
Cytoplasmic Foci | 0.0314 | 0.024 | 0.0311 | 0.0191 | 0.0301 | 0.016 | 0.0131 | 0.0168 | 0.0126 | 0.0059 | 0.008 | 0.0074 | 0.0257 | 0.0227 | 0.0134 | 0.0188 | 0.0097 | 0.0084 |
Eisosomes | 0.0009 | 0.0002 | 0.0005 | 0.0013 | 0.0021 | 0.0018 | 0.0006 | 0.0003 | 0.0003 | 0.0021 | 0.0003 | 0.0002 | 0.0019 | 0.0004 | 0.0014 | 0.0012 | 0.005 | 0.0003 |
Endoplasmic Reticulum | 0.0295 | 0.0239 | 0.0099 | 0.037 | 0.0247 | 0.0322 | 0.0261 | 0.01 | 0.0201 | 0.0122 | 0.0204 | 0.0165 | 0.0314 | 0.0065 | 0.0242 | 0.0427 | 0.0875 | 0.0262 |
Endosome | 0.0205 | 0.0033 | 0.0061 | 0.0376 | 0.0067 | 0.0097 | 0.0099 | 0.005 | 0.0025 | 0.0026 | 0.004 | 0.0041 | 0.016 | 0.0024 | 0.0104 | 0.0101 | 0.0024 | 0.0036 |
Golgi | 0.0107 | 0.0029 | 0.0091 | 0.0248 | 0.0137 | 0.0123 | 0.0073 | 0.0027 | 0.0032 | 0.0037 | 0.0058 | 0.003 | 0.0138 | 0.004 | 0.0056 | 0.0132 | 0.0069 | 0.0061 |
Lipid Particles | 0.6744 | 0.7879 | 0.7186 | 0.6403 | 0.6794 | 0.7462 | 0.7634 | 0.8663 | 0.7942 | 0.8098 | 0.8162 | 0.9093 | 0.7286 | 0.8498 | 0.7613 | 0.6469 | 0.6546 | 0.8611 |
Mitochondria | 0.006 | 0.0001 | 0.0028 | 0.0171 | 0.0001 | 0.0016 | 0.004 | 0.0001 | 0.0057 | 0.0011 | 0.0021 | 0.0001 | 0.0115 | 0.0001 | 0.0042 | 0.0117 | 0.0205 | 0.002 |
None | 0.0005 | 0.0001 | 0.0006 | 0.0015 | 0.0002 | 0.001 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0006 | 0.0005 | 0.0033 | 0.0001 |
Nuclear Periphery | 0.0018 | 0.0007 | 0.0012 | 0.0082 | 0.0022 | 0.003 | 0.0024 | 0.0007 | 0.0009 | 0.0006 | 0.0007 | 0.0003 | 0.0037 | 0.001 | 0.0039 | 0.0031 | 0.0015 | 0.0016 |
Nucleolus | 0.0005 | 0.001 | 0.0006 | 0.0004 | 0.0002 | 0.0006 | 0.0047 | 0.0009 | 0.003 | 0.0015 | 0.0003 | 0.0002 | 0.001 | 0.0007 | 0.0009 | 0.0004 | 0.0002 | 0.0003 |
Nucleus | 0.0004 | 0.0005 | 0.0006 | 0.001 | 0.0003 | 0.0008 | 0.0019 | 0.0003 | 0.002 | 0.0026 | 0.0002 | 0.0001 | 0.0059 | 0.0197 | 0.0086 | 0.0011 | 0.0002 | 0.0124 |
Peroxisomes | 0.0024 | 0.0004 | 0.0055 | 0.0005 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0008 | 0.0002 | 0.0013 | 0.0003 | 0.0061 | 0.0005 | 0.0003 | 0.0241 | 0.0012 | 0.0006 |
Punctate Nuclear | 0.0005 | 0.0007 | 0.001 | 0.0005 | 0.0004 | 0.0012 | 0.0015 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0025 | 0.001 | 0.0016 | 0.0005 | 0.0002 | 0.0004 |
Vacuole | 0.0244 | 0.0177 | 0.0184 | 0.0305 | 0.0079 | 0.013 | 0.025 | 0.0215 | 0.0152 | 0.0285 | 0.0132 | 0.0094 | 0.0192 | 0.0072 | 0.0201 | 0.034 | 0.0127 | 0.0079 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0003 | 0.004 | 0.0002 | 0.0004 | 0.0009 | 0.0006 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.002 | 0.0001 | 0.0023 | 0.003 | 0.0004 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 114.938 | 45.7693 | 63.7669 | 76.6952 | 103.6381 | 121.1402 | 86.652 | 89.8777 | 103.4801 | 134.3238 |
Translational Efficiency | 2.1291 | 2.3424 | 1.6649 | 2.1483 | 1.8531 | 2.6614 | 1.9811 | 1.8262 | 1.7951 | 1.8642 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2097 | 121 | 2794 | 2001 | 1601 | 1632 | 949 | 1442 | 3698 | 1753 | 3743 | 3443 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1916.01 | 2494.64 | 2168.72 | 2035.48 | 2092.93 | 2136.38 | 2295.09 | 1993.33 | 1992.61 | 2161.11 | 2200.76 | 2017.83 |
Standard Deviation | 335.32 | 347.58 | 357.55 | 415.53 | 347.88 | 355.85 | 385.85 | 442.55 | 351.90 | 366.71 | 369.05 | 427.56 |
Intensity Change Log 2 | — | 0.380727 | 0.178739 | 0.087264 | — | 0.029644 | 0.133027 | -0.070343 | — | 0.208109 | 0.155055 | 0.007133 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.018266 | 0.009794 | 0.102809* | 0.183655* | 0.005604 | 0.021074 | 0.067071 | 0.161954* | 0.012784 | 0.020295 | 0.093748 | 0.174566* |
Bud Neck | 0.005341 | 0.014873 | 0.009970 | 0.012616 | 0.005810 | 0.010985 | 0.023072 | 0.013840 | 0.005544 | 0.011254 | 0.013292 | 0.013129 |
Bud Site | 0.023826 | 0.025839 | 0.017672 | 0.042213 | 0.013319 | 0.020179 | 0.031142 | 0.060820 | 0.019277 | 0.020569 | 0.021087 | 0.050006 |
Cell Periphery | 0.043292 | 0.061534 | 0.006202 | 0.002816 | 0.166268* | 0.024338 | 0.011422 | 0.002564 | 0.096533 | 0.026905 | 0.007525 | 0.002711 |
Cytoplasm | 0.035687 | 0.002052 | 0.002957 | 0.007772 | 0.015249 | 0.011002 | 0.004049 | 0.015170 | 0.026839 | 0.010385 | 0.003234 | 0.010871 |
Cytoplasmic Foci | 0.013826 | 0.004663 | 0.009456 | 0.044514 | 0.002432 | 0.006450 | 0.036853 | 0.085517 | 0.008893 | 0.006327 | 0.016402 | 0.061687 |
Eisosomes | 0.001439 | 0.000942 | 0.002605 | 0.000648 | 0.001466 | 0.001709 | 0.000875 | 0.000463 | 0.001451 | 0.001656 | 0.002167 | 0.000570 |
Endoplasmic Reticulum | 0.033492 | 0.028013 | 0.007729 | 0.004862 | 0.076909 | 0.020391 | 0.007352 | 0.002977 | 0.052289 | 0.020917 | 0.007634 | 0.004073 |
Endosome | 0.013507 | 0.017863 | 0.038355 | 0.128838 | 0.008360 | 0.012437 | 0.065936 | 0.144782 | 0.011279 | 0.012812 | 0.045348 | 0.135516 |
Golgi | 0.015497 | 0.015120 | 0.009779 | 0.065195 | 0.008577 | 0.007295 | 0.018583 | 0.050484 | 0.012501 | 0.007836 | 0.012011 | 0.059034 |
Lipid Particles | 0.776907* | 0.785933* | 0.776663* | 0.466418* | 0.654386* | 0.851374* | 0.675350* | 0.384018* | 0.723863* | 0.846857* | 0.750976* | 0.431907* |
Mitochondria | 0.000825 | 0.000208 | 0.000249 | 0.000653 | 0.000377 | 0.000286 | 0.000036 | 0.000636 | 0.000631 | 0.000281 | 0.000195 | 0.000646 |
Mitotic Spindle | 0.000818 | 0.000436 | 0.000799 | 0.004998 | 0.001241 | 0.000547 | 0.007578 | 0.021044 | 0.001001 | 0.000540 | 0.002517 | 0.011718 |
None | 0.001123 | 0.000029 | 0.000050 | 0.000071 | 0.000248 | 0.000674 | 0.000022 | 0.000044 | 0.000744 | 0.000629 | 0.000043 | 0.000060 |
Nuclear Periphery | 0.000474 | 0.001386 | 0.000093 | 0.000250 | 0.002963 | 0.000262 | 0.000273 | 0.000263 | 0.001552 | 0.000340 | 0.000138 | 0.000256 |
Nuclear Periphery Foci | 0.002061 | 0.005025 | 0.000828 | 0.003096 | 0.003741 | 0.001769 | 0.005281 | 0.004330 | 0.002788 | 0.001994 | 0.001957 | 0.003613 |
Nucleolus | 0.000021 | 0.000012 | 0.000004 | 0.000019 | 0.000030 | 0.000008 | 0.000010 | 0.000016 | 0.000025 | 0.000009 | 0.000005 | 0.000017 |
Nucleus | 0.000450 | 0.000162 | 0.000082 | 0.000133 | 0.000504 | 0.000253 | 0.000151 | 0.000170 | 0.000473 | 0.000246 | 0.000100 | 0.000149 |
Peroxisomes | 0.006512 | 0.000468 | 0.007433 | 0.008969 | 0.005094 | 0.001772 | 0.001919 | 0.013184 | 0.005898 | 0.001682 | 0.006035 | 0.010735 |
Vacuole | 0.005310 | 0.022783 | 0.006029 | 0.020832 | 0.024381 | 0.006245 | 0.042555 | 0.036342 | 0.013566 | 0.007387 | 0.015290 | 0.027328 |
Vacuole Periphery | 0.001325 | 0.002865 | 0.000237 | 0.001430 | 0.003042 | 0.000949 | 0.000472 | 0.001383 | 0.002068 | 0.001081 | 0.000296 | 0.001410 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.70 | -19.48 | -24.92* | -22.01* | -10.36 | -6.29 | -10.97 | -21.13 | -18.18 | -9.69 | -3.24 | -23.28 | -32.64* | -29.05* | -13.03 |
Bud Neck | -2.16 | -5.72 | -8.94 | 0.49 | -2.64 | -4.57 | -11.62 | -9.66 | -2.82 | 5.61 | -5.54 | -10.80 | -12.95 | -2.05 | -0.07 |
Bud Site | -0.55 | 2.51 | -7.80 | -1.84 | -10.36 | -2.79 | -6.75 | -14.85 | -12.65 | -8.02 | -0.79 | -2.06 | -15.92 | -12.85 | -14.48 |
Cell Periphery | -1.21 | 13.98 | 15.56 | 4.00 | 4.91 | 21.93* | 24.56* | 26.85* | 9.81 | 5.01 | 17.78 | 27.51 | 29.46 | 10.51 | 7.11 |
Cytoplasm | 11.50 | 11.98 | 8.90 | -4.88 | -5.84 | 1.59 | 5.33 | -2.74 | -4.02 | -7.13 | 7.00 | 13.45 | 5.72 | -2.23 | -9.69 |
Cytoplasmic Foci | 3.94 | 3.65 | -14.52 | -14.51 | -17.29 | -5.12 | -10.75 | -24.72 | -23.20 | -9.52 | 2.83 | -7.15 | -27.78 | -28.62 | -21.33 |
Eisosomes | 1.85 | -4.11 | 6.95 | 1.24 | 7.54 | -0.61 | 3.88 | 8.70 | 3.36 | 3.45 | -0.56 | -3.32 | 10.81 | 3.13 | 8.15 |
Endoplasmic Reticulum | 0.83 | 11.87 | 13.81 | 3.48 | 3.75 | 13.87 | 18.68 | 20.34 | 9.52 | 5.12 | 11.78 | 21.60 | 24.16 | 9.52 | 5.99 |
Endosome | -1.55 | -13.40 | -29.29* | -23.35 | -21.28 | -4.21 | -16.19 | -27.24 | -25.97 | -12.04 | -1.62 | -20.93 | -40.01* | -38.40* | -25.59 |
Golgi | 0.08 | 4.42 | -17.36 | -10.90 | -20.74* | 1.11 | -5.54 | -14.75 | -15.21 | -9.75 | 4.31 | 0.63 | -22.65 | -24.81 | -23.51* |
Lipid Particles | -0.14 | 0.23 | 30.67 | 10.96 | 32.36* | -18.04* | -0.55 | 22.41 | 43.73* | 20.58* | -14.75 | -3.04 | 37.11 | 48.82* | 41.18* |
Mitochondria | 4.49 | 5.13 | 1.01 | -2.72 | -2.81 | 1.37 | 7.97 | -1.15 | -1.63 | -3.11 | 4.55 | 6.21 | 0.04 | -2.92 | -3.77 |
Mitotic Spindle | 2.13 | -0.28 | -5.14 | -5.39 | -4.85 | 5.22 | -3.78 | -9.28 | -9.54 | -4.46 | 3.97 | -3.42 | -10.52 | -10.83 | -7.90 |
None | 4.40 | 4.31 | 4.19 | -3.31 | -1.81 | -1.36 | 5.99 | 5.16 | 1.95 | -2.73 | 0.29 | 4.91 | 4.73 | 1.89 | -2.34 |
Nuclear Periphery | -1.55 | 8.98 | 2.40 | 1.89 | -2.40 | 16.64* | 16.54 | 16.43 | -1.29 | -0.58 | 13.56 | 18.41 | 14.45 | 0.86 | -3.21 |
Nuclear Periphery Foci | -1.52 | 7.75 | -2.84 | 0.87 | -5.82 | 3.63 | -2.26 | -1.98 | -5.44 | 0.96 | 1.95 | 1.74 | -3.28 | -4.53 | -4.41 |
Nucleolus | 1.66 | 7.21 | 0.11 | -0.90 | -2.14 | 7.58 | 2.46 | 3.65 | -5.40 | -0.80 | 7.96 | 7.90 | 1.12 | -2.36 | -2.79 |
Nucleus | 2.70 | 9.01 | 2.76 | -0.47 | -2.51 | 2.68 | 5.64 | 0.77 | -0.89 | -1.84 | 2.77 | 11.59 | 2.41 | -0.28 | -3.35 |
Peroxisomes | 9.40 | -0.97 | -2.07 | -9.23 | -1.23 | 4.22 | 3.61 | -5.02 | -8.29 | -7.82 | 7.72 | -0.16 | -5.00 | -11.00 | -4.76 |
Vacuole | -2.73 | -1.39 | -11.69 | 0.08 | -10.75 | 13.24 | -5.90 | -7.35 | -14.35 | -0.36 | 6.40 | -2.20 | -12.41 | -15.77 | -9.77 |
Vacuole Periphery | -0.78 | 3.02 | -0.29 | 0.72 | -5.06 | 2.33 | 3.02 | 1.95 | -1.01 | -3.63 | 1.89 | 4.20 | 1.48 | -0.90 | -6.45 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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