Standard name
Human Ortholog
Description Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.07 0.07 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.09 0.06 0.05 0.06 0.05 0 0.05 0 0.06 0.18 0.16 0.06 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0 0 0.09 0.24 0.17 0.28 0.22 0.27 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.89 0.93 0.89 0.84 0.84 0.72 0.77 0.74 0.74 0.72 0.75 0.89 0.8 0.81 0.84 0.89 0.89 0.84 0.82 0.83 0.71 0.67 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.33 0.08 0.14 0.08 0.19 0.3 0.25 0.33 0.32 0.36 0.31 0 0.05 0 0.09 0.12 0.17 0.08 0.1 0.1 0.18 0.17 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Bud 0 0 0 0 0 0 1 3 1 3 3 0 1 0 0 1 0 1 1 0 1 1 3 3
Bud Neck 0 0 0 0 0 0 0 4 1 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 1 4 13 11 19 18 10 15 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 3 6 8 23 15 15 18 13 7 16 13 15 51 41 20 16 8 1 1 1 3 1 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 3 3 2 0 0 0 1 1 1
Endosome 0 0 2 0 0 1 0 0 0 0 0 0 0 2 2 2 2 1 1 0 0 1 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 0 0 1 0
Mitochondria 25 2 3 7 4 23 77 51 79 54 81 44 5 0 1 5 4 8 0 0 1 1 1 2
Nucleus 165 100 194 169 227 211 232 232 210 182 218 233 229 234 204 302 279 237 158 94 186 153 176 189
Nuclear Periphery 2 0 1 0 1 1 1 0 3 2 2 0 1 1 1 4 2 0 1 0 0 1 0 0
Nucleolus 61 37 17 27 22 48 95 74 94 79 109 97 10 14 9 33 39 46 14 11 22 39 43 61
Peroxisomes 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 4 0 1 0 0 0 0 0 0
SpindlePole 0 0 6 1 3 3 3 2 0 5 1 4 2 4 5 6 1 0 1 1 0 2 4 6
Vac/Vac Membrane 0 0 0 0 4 1 1 2 1 1 2 3 4 3 1 9 2 3 2 4 6 5 21 12
Unique Cell Count 186 112 208 189 270 251 321 301 284 245 303 312 256 291 253 360 315 265 188 115 224 216 264 288
Labelled Cell Count 257 142 231 213 286 307 438 397 422 353 444 411 268 309 265 390 348 309 188 115 224 216 264 288


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 8.2 6.8 6.5 7.8 5.9 5.4 5.6 5.5 5.6 5.3 5.8 7.6 8.1 7.9 11.5 11.1 10.8 8.2 8.1 8.7
Std Deviation (1e-4) 1.1 1.8 1.2 1.3 2.9 1.7 1.5 1.4 1.8 2.2 2.0 1.6 2.4 3.0 2.9 2.7 3.0 3.0 2.4 3.0 2.3
Intensity Change (Log2) – – – -0.06 0.2 -0.21 -0.33 -0.28 -0.32 -0.29 -0.36 -0.23 0.16 0.24 0.22 0.76 0.7 0.66 0.26 0.26 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.5 0 3.4 3.6 0 2.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 2.6 1.6 1.0 1.6 1.0 0 1.3 0.8 1.5 5.1 4.7 1.5 1.2 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 7.1 5.6 7.8 6.6 7.6 4.9 0 0 0 0 0 0
Nucleus -1.4 -3.1 -3.0 -6.0 -4.9 -5.5 -5.4 -6.0 -5.4 -1.4 -4.1 -3.9 -3.2 -1.8 -1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.9 0 3.3 5.9 4.8 6.5 6.2 7.2 6.2 -2.0 -1.5 -2.1 0.4 1.5 2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 -0.6 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5393 2.1365 2.0515 1.3547 1.0507 1.6778 3.6333 3.15 3.2052 3.0275 3.1049 3.1071 3.5728 2.4575 2.2809 2.65 3.0535 2.5559
Actin 0.0106 0.0017 0.0009 0 0.0435 0 0.0112 0 0.0017 0 0.0043 0 0.0103 0 0.0051 0.0068 0.0057 0.0019
Bud 0.0004 0.0001 0 0 0.0004 0 0.0003 0 0.0001 0 0.0003 0 0.0003 0 0.0003 0.0002 0.0003 0
Bud Neck 0.001 0.0004 0.0003 0.0001 0.0006 0.0004 0.0013 0 0.0005 0 0.0013 0.0003 0.0008 0 0.0005 0.0004 0.0005 0.0004
Bud Periphery 0.0009 0.0001 0.0001 0.0001 0.0016 0 0.001 0 0.0002 0 0.001 0 0.0005 0 0.0007 0.0003 0.0008 0.0001
Bud Site 0.0073 0.0024 0.0003 0.0002 0.0007 0 0.0011 0 0.0007 0 0.0012 0 0.0021 0 0.0006 0.0005 0.0007 0.0001
Cell Periphery 0.0005 0 0 0.0001 0.0003 0 0.0005 0 0.0001 0 0.0003 0 0.0002 0 0.0002 0 0.0002 0
Cytoplasm 0.0051 0.0001 0.0069 0.0014 0.0003 0.0004 0.0013 0 0.0011 0 0.0004 0 0.0032 0 0.0011 0.0001 0.0001 0.0002
Cytoplasmic Foci 0.0031 0.0001 0.0035 0.0016 0.0048 0 0.0054 0 0.0027 0 0.0048 0.0002 0.0113 0 0.0009 0.0069 0.0026 0.0016
Eisosomes 0.0003 0 0 0 0.0006 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0041 0 0.0005 0.0005 0.0006 0 0.0011 0 0.0009 0 0.0008 0 0.0012 0 0.0009 0.0001 0.0003 0.0004
Endosome 0.0154 0 0.0043 0.0219 0.0102 0.0003 0.0094 0 0.0039 0 0.0057 0.0004 0.0145 0 0.003 0.0027 0.0079 0.0039
Golgi 0.0023 0.0001 0.0008 0.0038 0.0075 0 0.002 0 0.0007 0 0.0027 0.0002 0.0044 0 0.0011 0.0046 0.0071 0.001
Lipid Particles 0.0032 0 0.0042 0.0003 0.0158 0 0.0164 0 0.0059 0 0.0085 0.0028 0.0163 0 0.0009 0.0107 0.0155 0.0021
Mitochondria 0.0031 0 0.0017 0.0035 0.0114 0.0001 0.0062 0 0.0015 0 0.0023 0.0007 0.0058 0 0.0114 0.0024 0.0136 0.0003
None 0.0032 0 0.0006 0.0005 0.0008 0.0007 0.0066 0 0.0014 0 0.0006 0 0.002 0 0.0004 0.0002 0.0003 0.0002
Nuclear Periphery 0.016 0.0006 0.0079 0.0041 0.0054 0.0031 0.0072 0.0008 0.0066 0.0003 0.0119 0.0005 0.011 0.0007 0.0024 0.001 0.0029 0.0049
Nucleolus 0.0464 0.0167 0.019 0.0299 0.0737 0.0996 0.0423 0.027 0.0152 0.0189 0.1592 0.0877 0.043 0.0203 0.0274 0.0137 0.1664 0.0913
Nucleus 0.857 0.9758 0.937 0.9256 0.7812 0.8923 0.8628 0.9668 0.9503 0.98 0.7766 0.9037 0.8556 0.967 0.9318 0.8961 0.7679 0.8844
Peroxisomes 0.0005 0 0.0036 0.0001 0.0228 0 0.0028 0 0.0023 0 0.0021 0.0005 0.0041 0 0.0007 0.0114 0.003 0.004
Punctate Nuclear 0.0146 0.0018 0.0075 0.0042 0.0138 0.0027 0.0166 0.0053 0.0023 0.0008 0.013 0.0026 0.0107 0.0118 0.0082 0.0417 0.0023 0.0026
Vacuole 0.0031 0 0.0005 0.0011 0.002 0.0001 0.0032 0 0.0011 0 0.0022 0.0001 0.0015 0 0.0014 0.0001 0.0009 0.0002
Vacuole Periphery 0.0018 0 0.0006 0.0007 0.0021 0.0001 0.0012 0 0.0008 0 0.0009 0 0.0008 0 0.001 0.0001 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.4374 10.2427 9.8807 10.8602 9.6563 17.1775 8.2851 11.8828 15.5068 11.2695
Translational Efficiency 18.8806 4.8403 5.2498 6.1947 5.2606 3.6386 4.8019 3.7165 3.1135 4.3552

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
82 489 1853 1544 1109 2256 1200 832 1191 2745 3053 2376

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 925.12 922.60 1122.62 1122.49 835.20 912.58 1083.77 1146.49 841.39 914.36 1107.35 1130.89
Standard Deviation 141.24 176.36 168.30 180.97 132.96 139.04 159.94 200.68 135.48 146.44 166.15 188.45
Intensity Change Log 2 — -0.003935 0.279157 0.278990 — 0.127829 0.375865 0.457030 — 0.060084 0.325850 0.366206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000460 0.000970 0.000787 0.000891 0.000622 0.000420 0.000777 0.001568 0.000611 0.000518 0.000783 0.001128
Bud Neck 0.002886 0.013874 0.029111 0.024979 0.009383 0.011049 0.016574 0.026964 0.008936 0.011553 0.024183 0.025674
Bud Site 0.000323 0.002970 0.001381 0.001954 0.001286 0.001638 0.000965 0.004926 0.001220 0.001875 0.001218 0.002995
Cell Periphery 0.000018 0.000206 0.000039 0.000069 0.000084 0.000076 0.000030 0.000114 0.000079 0.000099 0.000035 0.000084
Cytoplasm 0.000029 0.001138 0.000157 0.001512 0.001531 0.000949 0.000090 0.001478 0.001428 0.000982 0.000131 0.001500
Cytoplasmic Foci 0.000016 0.000877 0.000016 0.000766 0.001430 0.000513 0.000013 0.001858 0.001333 0.000578 0.000015 0.001148
Eisosomes 0.000037 0.000049 0.000058 0.000043 0.000037 0.000055 0.000058 0.000045 0.000037 0.000054 0.000058 0.000043
Endoplasmic Reticulum 0.002326 0.003123 0.001294 0.001901 0.002808 0.003015 0.001906 0.001957 0.002775 0.003034 0.001534 0.001921
Endosome 0.000042 0.001148 0.000049 0.000693 0.000578 0.000804 0.000053 0.002854 0.000541 0.000865 0.000051 0.001449
Golgi 0.000049 0.000377 0.000110 0.001316 0.000334 0.000365 0.000087 0.002596 0.000314 0.000367 0.000101 0.001764
Lipid Particles 0.000035 0.000196 0.000016 0.000059 0.000498 0.000134 0.000025 0.000354 0.000466 0.000145 0.000019 0.000162
Mitochondria 0.000534 0.001283 0.001549 0.002795 0.002127 0.001424 0.001041 0.005359 0.002017 0.001399 0.001349 0.003693
Mitotic Spindle 0.000068 0.002822 0.000689 0.006790 0.003217 0.004258 0.001054 0.016395 0.003000 0.004002 0.000833 0.010153
None 0.000776 0.005429 0.001449 0.001722 0.001913 0.001989 0.001064 0.006936 0.001835 0.002602 0.001298 0.003548
Nuclear Periphery 0.000031 0.000135 0.000125 0.001208 0.000199 0.000392 0.000084 0.001176 0.000188 0.000346 0.000109 0.001197
Nuclear Periphery Foci 0.000029 0.000656 0.000029 0.000545 0.000326 0.000244 0.000044 0.000561 0.000305 0.000318 0.000035 0.000551
Nucleolus 0.059313 0.082861 0.059442 0.040185 0.076526 0.064068 0.056289 0.047178 0.075341 0.067416 0.058203 0.042634
Nucleus 0.932107* 0.878945* 0.902356* 0.907206* 0.895442* 0.906162* 0.918443* 0.868889* 0.897966* 0.901314* 0.908679* 0.893789*
Peroxisomes 0.000743 0.001217 0.000932 0.000675 0.000863 0.000632 0.000974 0.001643 0.000855 0.000736 0.000949 0.001014
Vacuole 0.000109 0.001297 0.000236 0.002498 0.000457 0.001113 0.000294 0.004138 0.000433 0.001146 0.000259 0.003072
Vacuole Periphery 0.000070 0.000425 0.000174 0.002195 0.000340 0.000700 0.000133 0.003013 0.000321 0.000651 0.000158 0.002482

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.77 -8.35 -9.11 -0.40 -1.85 3.24 -3.11 -5.72 -7.10 -4.89 1.65 -4.22 -7.07 -10.04 -5.23
Bud Neck -6.40 -26.74 -19.45 -6.82 1.89 -1.60 -7.90 -11.72 -11.21 -7.29 -2.98 -18.84 -17.54 -15.92 -3.16
Bud Site -3.07 -12.84 -7.24 1.14 -1.94 -0.52 0.87 -4.34 -3.94 -4.88 -1.42 -0.65 -4.58 -2.77 -5.27
Cell Periphery -2.72 -4.68 -5.98 1.88 -3.63 0.48 2.93 -1.47 -2.85 -6.67 -0.85 2.53 -0.53 0.56 -6.94
Cytoplasm -3.32 -3.27 -2.39 -0.79 -2.20 0.65 2.60 0.39 -0.49 -6.15 0.61 2.54 0.11 -0.61 -3.30
Cytoplasmic Foci -1.25 -1.27 -1.52 0.42 -1.50 1.53 2.33 0.71 -1.21 -2.61 1.38 2.32 1.16 -0.37 -2.69
Eisosomes -2.51 -6.37 -1.60 1.70 9.34 -6.24 -9.46 -5.66 0.45 2.49 -6.63 -12.55 -4.87 2.99 8.87
Endoplasmic Reticulum -2.34 3.86 1.35 4.17 -6.22 -0.70 4.19 1.05 1.72 -2.87 -1.06 7.04 3.46 4.87 -5.98
Endosome -1.19 -1.56 -2.74 0.64 -2.65 -0.80 2.25 -1.77 -1.38 -2.83 -1.05 2.25 -1.21 -0.14 -3.78
Golgi -2.44 -9.99 -1.92 -1.18 -1.74 -0.14 2.16 -2.16 -2.07 -2.64 -0.30 1.91 -2.24 -2.06 -2.87
Lipid Particles -1.77 1.32 -1.24 1.48 -2.06 1.25 1.66 0.65 -1.27 -2.37 1.19 1.68 1.22 0.10 -2.82
Mitochondria -6.46 -13.57 -11.07 -7.17 -5.49 0.92 1.39 -2.36 -3.57 -4.05 0.87 0.72 -2.21 -5.36 -6.02
Mitotic Spindle -2.06 -4.21 -5.58 -3.22 -4.89 -0.30 1.94 -5.18 -5.14 -6.05 -0.37 1.99 -5.62 -5.71 -7.73
None -2.40 -1.77 -4.05 1.75 -1.39 -0.03 2.51 -2.12 -2.11 -2.76 -1.29 1.60 -1.95 -0.75 -3.14
Nuclear Periphery -4.72 -10.91 -3.67 -3.30 -3.27 -1.44 3.36 -5.87 -4.23 -6.49 -1.43 2.14 -4.82 -3.78 -5.15
Nuclear Periphery Foci -1.57 -0.49 -2.45 0.50 -2.41 0.57 3.32 -0.82 -1.31 -3.52 -0.07 3.43 -0.75 -0.64 -3.58
Nucleolus -2.35 -0.97 1.25 6.86 5.86 3.33 4.07 1.64 -0.91 -1.77 2.25 2.95 5.55 4.34 3.49
Nucleus 4.90 4.57 4.07 -1.75 -0.50 -2.21 -3.61 6.60 8.52 9.43 -0.84 -0.36 4.34 6.04 5.94
Peroxisomes -2.83 -3.31 -0.14 3.22 4.47 1.24 -0.85 -3.58 -5.01 -3.58 0.72 -1.41 -1.56 -3.35 -0.48
Vacuole -2.58 -8.59 -3.30 -0.92 -2.97 -2.07 0.57 -3.87 -3.04 -3.99 -2.51 0.79 -4.52 -2.79 -4.89
Vacuole Periphery -3.63 -12.94 -3.13 -2.37 -2.89 -1.83 3.21 -1.89 -1.37 -2.15 -2.00 2.61 -3.23 -2.32 -3.61
Standard name
Human Ortholog
Description Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ies2

Ies2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ies2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available