Standard name
Human Ortholog
Description RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.08 0 0 0 0.07 0.16 0 – – 0 0.06 0.14 0.1 0.07 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.15 0 0 0 0.05 – – 0 0 0 0.08 0.08 0.07 0.06 0 0 0 0 0
Nucleus 0.56 0.77 0.63 0.57 0.69 0.54 0.62 0.6 0.62 0.6 – – 0.72 0.56 0.45 0.56 0.58 0.58 0.49 0.66 0.47 0.3 0.33 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.75 0.66 0.47 0.3 0.33 0.53 0.38 0.4 0.33 0.51 – – 0.58 0.56 0.54 0.46 0.42 0.33 0.25 0.21 0.31 0.5 0.49 0.47
Peroxisomes 0.06 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.06 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0 0 0 – – 0 0 0 0 0.05 0.05 0.05 0.05 0.05 0.07 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 1 1 1 – – 0 0 0 0 0 0 2 2 9 5 4 3
Bud Neck 0 1 3 1 0 0 0 0 0 1 – – 0 1 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 1 2 0 1 2 2 1 1 – – 2 3 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 10 8 4 4 6 17 10 4 – – 7 12 22 15 9 24 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 – – 0 0 0 3 1 1 0 1 0 0 0 0
Endosome 0 0 2 1 2 0 3 2 1 0 – – 0 3 1 4 2 2 1 2 4 1 1 0
Golgi 0 0 1 0 0 0 1 3 0 0 – – 0 0 0 0 0 0 0 0 0 0 1 3
Mitochondria 2 3 3 1 0 22 2 5 2 7 – – 1 2 1 12 10 13 3 0 4 2 6 4
Nucleus 9 93 112 55 94 79 112 147 38 77 – – 113 107 69 84 77 101 27 90 103 31 77 53
Nuclear Periphery 0 0 0 0 0 1 0 1 0 3 – – 1 2 0 0 0 0 0 0 4 0 1 1
Nucleolus 12 80 84 29 45 78 68 98 20 65 – – 91 107 83 69 55 58 13 28 68 51 113 78
Peroxisomes 1 0 0 0 1 0 0 0 1 0 – – 0 3 4 1 0 0 0 0 1 0 0 0
SpindlePole 1 0 10 3 2 1 2 4 2 1 – – 3 4 5 0 0 0 0 0 4 0 5 3
Vac/Vac Membrane 0 1 6 4 5 3 10 5 1 0 – – 2 3 0 2 7 9 2 6 10 7 13 13
Unique Cell Count 16 121 179 96 137 147 180 246 61 128 157 192 153 151 132 175 55 136 220 103 232 168
Labelled Cell Count 25 180 232 104 153 189 206 286 77 160 220 247 186 191 161 208 55 136 220 103 232 168


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 15.0 17.9 17.5 17.2 11.0 15.5 15.5 13.4 14.0 – – 19.0 18.8 17.5 16.4 20.4 18.3 14.7 16.3 17.3
Std Deviation (1e-4) 6.1 5.4 6.6 6.7 5.8 4.5 5.7 5.4 4.8 4.9 – – 6.7 7.5 7.4 6.9 9.3 8.6 7.5 5.2 5.1
Intensity Change (Log2) – – – -0.04 -0.06 -0.7 -0.21 -0.21 -0.42 -0.36 – – 0.08 0.07 -0.04 -0.13 0.18 0.02 -0.29 -0.13 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.9 -1.1 -1.3 -1.0 0.6 2.6 -1.0 – – -0.5 0.3 2.7 1.5 0.4 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 4.5 0 0 0 0 – – 0 0 0 2.7 2.6 2.6
Nucleus -0.9 1.1 -1.6 -0.1 -0.6 0 -0.4 – – 1.8 -1.3 -3.2 -1.3 -0.8 -0.9
Nuclear Periphery 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus -2.7 -2.5 1.1 -1.8 -1.5 -1.9 0.7 – – 2.0 1.7 1.3 -0.2 -0.9 -2.6
Peroxisomes 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 -1.9 0 -2.4 -2.3 0 0 – – -1.7 -1.8 -1.0 0 0 0
Vacuole 0 0 0 1.0 0 0 0 – – 0 0 0 0 0.8 0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.1496 11.3762 9.7317 9.1959 9.1844 9.1274 12.631 15.7464 14.2986 12.4989 9.7648 13.8741 16.6765 20.0416 18.0439 17.123 17.9384 17.6855
Actin 0.0494 0.0012 0.0238 0.002 0.0081 0.0035 0.044 0 0.0076 0.0011 0.0383 0.0002 0.02 0 0 0 0.0001 0
Bud 0.002 0 0.0013 0.0002 0.0002 0.0002 0.0017 0.0001 0.0005 0.0023 0.0031 0.0001 0.0003 0 0 0 0.0001 0
Bud Neck 0.0011 0 0.0012 0.0001 0.0004 0.0005 0.0019 0 0.0038 0.0004 0.0019 0.0002 0.0004 0 0 0 0.0008 0.0002
Bud Periphery 0.0031 0 0.0018 0.0003 0.0005 0.0006 0.0023 0.0003 0.0003 0.0013 0.003 0.0001 0.0004 0 0 0 0.0005 0
Bud Site 0.0042 0 0.0024 0.0004 0.0007 0.0002 0.0114 0.0001 0.011 0.0041 0.0059 0.0001 0.0003 0 0 0 0.0002 0.0001
Cell Periphery 0.0015 0 0.0003 0.0001 0.0001 0.0001 0.0011 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0 0 0 0.0013 0.0001
Cytoplasm 0.0289 0.0001 0.001 0.001 0.0026 0.0001 0.0094 0 0.0119 0.0099 0.0117 0 0.0047 0 0 0 0 0.0002
Cytoplasmic Foci 0.0386 0.0002 0.0032 0.0017 0.0288 0.0006 0.0215 0.0024 0.0036 0.0006 0.0606 0.0011 0.0048 0 0 0 0 0.0019
Eisosomes 0.0008 0 0.0002 0.0001 0.0005 0.0001 0.0004 0 0 0 0.0004 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0067 0.0003 0.0009 0.0008 0.0009 0.0003 0.0059 0 0.0002 0.0007 0.0051 0 0.0025 0 0 0 0 0
Endosome 0.0338 0.0017 0.0087 0.001 0.0075 0.0022 0.0274 0.0001 0.0005 0.0005 0.0813 0.0004 0.012 0 0 0 0.0001 0.0001
Golgi 0.01 0.0004 0.0035 0.0003 0.002 0.0012 0.0078 0 0.001 0.0001 0.0403 0.0008 0.0021 0 0 0 0 0
Lipid Particles 0.0315 0.0003 0.0023 0.0095 0.0109 0.0026 0.0191 0.0029 0.0013 0.0001 0.1193 0.0008 0.0032 0 0 0 0.0001 0.001
Mitochondria 0.0219 0.0005 0.0194 0.0514 0.0627 0.0144 0.0129 0.0059 0.0004 0.0027 0.0379 0.0191 0.006 0.0001 0.0002 0.0004 0.0113 0.0002
None 0.0203 0.0001 0.0006 0.0006 0.0048 0.0003 0.0098 0 0.0001 0.0006 0.0026 0.0001 0.0217 0 0 0 0 0.0001
Nuclear Periphery 0.0551 0.0047 0.0072 0.024 0.026 0.0176 0.0456 0.0008 0.0005 0.0169 0.0169 0.0001 0.0517 0 0.0002 0.0001 0.0001 0.0006
Nucleolus 0.1904 0.0842 0.1077 0.11 0.1378 0.2566 0.1126 0.0768 0.1039 0.1611 0.1813 0.304 0.1663 0.0748 0.0769 0.0519 0.5321 0.2929
Nucleus 0.4283 0.9048 0.7876 0.7857 0.649 0.6667 0.6079 0.9017 0.8475 0.7915 0.3372 0.6714 0.6938 0.925 0.9224 0.9475 0.4499 0.694
Peroxisomes 0.0082 0 0.0017 0.0011 0.0014 0.0008 0.0089 0.0006 0.0034 0.0005 0.0293 0.0001 0.0014 0 0 0 0.0001 0
Punctate Nuclear 0.0476 0.001 0.0221 0.0049 0.051 0.0302 0.038 0.0001 0.0017 0.004 0.0111 0.0005 0.0042 0 0.0001 0 0.0001 0.0085
Vacuole 0.0082 0.0002 0.0015 0.0009 0.001 0.0004 0.0057 0.0025 0.0004 0.001 0.0073 0.0003 0.001 0 0 0 0.0016 0.0001
Vacuole Periphery 0.0084 0.0003 0.0017 0.0038 0.0031 0.0009 0.0047 0.0054 0.0002 0.0005 0.0051 0.0004 0.0029 0 0 0 0.0015 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.6435 54.7987 45.059 62.664 79.4784 44.7078 51.6632 71.7597 72.8057 61.6573
Translational Efficiency 2.0405 2.2367 2.3391 2.4557 1.4512 2.3717 2.0444 1.66 1.6866 1.7223

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
43 75 1479 1316 751 677 1949 1055 794 752 3428 2371

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1425.85 1594.69 1430.52 1486.52 1090.37 1485.46 1575.84 1477.81 1108.54 1496.35 1513.14 1482.64
Standard Deviation 454.72 394.86 316.60 438.44 292.52 366.35 342.89 383.40 312.88 370.74 339.52 414.88
Intensity Change Log 2 — 0.161454 0.004717 0.060117 — 0.446092 0.531303 0.438643 — 0.291743 0.256760 0.236448

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001812 0.000738 0.000717 0.001186 0.000893 0.001116 0.000638 0.001155 0.000943 0.001078 0.000672 0.001172
Bud Neck 0.013207 0.005301 0.006711 0.010532 0.004969 0.009020 0.004235 0.019439 0.005415 0.008649 0.005304 0.014495
Bud Site 0.032559 0.000650 0.000621 0.002462 0.000886 0.002183 0.000499 0.005041 0.002601 0.002030 0.000552 0.003609
Cell Periphery 0.001651 0.000046 0.000023 0.000385 0.000078 0.000138 0.000034 0.000250 0.000163 0.000129 0.000029 0.000325
Cytoplasm 0.007272 0.000879 0.000029 0.001542 0.000442 0.001526 0.000092 0.002985 0.000812 0.001462 0.000065 0.002184
Cytoplasmic Foci 0.029850 0.000610 0.000098 0.002410 0.000872 0.002213 0.000363 0.006436 0.002442 0.002054 0.000249 0.004202
Eisosomes 0.000103 0.000069 0.000130 0.000107 0.000070 0.000103 0.000143 0.000100 0.000072 0.000099 0.000138 0.000104
Endoplasmic Reticulum 0.012695 0.000260 0.000206 0.000597 0.000563 0.000729 0.000156 0.000797 0.001220 0.000682 0.000178 0.000686
Endosome 0.039866 0.000059 0.000007 0.001300 0.000143 0.001898 0.000023 0.004096 0.002294 0.001714 0.000016 0.002544
Golgi 0.011331 0.000224 0.000049 0.002052 0.000684 0.000510 0.000049 0.002427 0.001261 0.000481 0.000049 0.002219
Lipid Particles 0.001986 0.000021 0.000003 0.000135 0.000046 0.001363 0.000006 0.000304 0.000152 0.001229 0.000004 0.000210
Mitochondria 0.032714 0.002267 0.001578 0.003895 0.002179 0.004838 0.001306 0.005333 0.003833 0.004582 0.001423 0.004535
Mitotic Spindle 0.021858 0.053083 0.000208 0.007859 0.007164 0.030317 0.000119 0.014557 0.007960 0.032587 0.000157 0.010840
None 0.001308 0.001946 0.000988 0.007859 0.002325 0.001356 0.000555 0.003868 0.002270 0.001415 0.000741 0.006083
Nuclear Periphery 0.000307 0.000433 0.000039 0.000491 0.000594 0.000473 0.000031 0.000764 0.000578 0.000469 0.000034 0.000613
Nuclear Periphery Foci 0.000741 0.000250 0.000006 0.000668 0.000499 0.000979 0.000023 0.001399 0.000512 0.000906 0.000016 0.000993
Nucleolus 0.079042 0.301262* 0.111028 0.102011 0.183708 0.189640 0.123152 0.089399 0.178040 0.200772 0.117921 0.096399
Nucleus 0.616982* 0.616136* 0.871119* 0.839852* 0.784200* 0.737558* 0.862561* 0.827687* 0.775145* 0.725448* 0.866253* 0.834439*
Peroxisomes 0.009855 0.015071 0.005943 0.007719 0.007777 0.011332 0.005576 0.005910 0.007889 0.011705 0.005734 0.006914
Vacuole 0.058337 0.000235 0.000130 0.003155 0.000794 0.001395 0.000133 0.004859 0.003910 0.001279 0.000131 0.003913
Vacuole Periphery 0.026524 0.000459 0.000368 0.003783 0.001113 0.001314 0.000305 0.003193 0.002489 0.001229 0.000332 0.003520

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.59 2.77 1.42 -4.82 -8.70 -1.81 5.07 -3.69 -0.88 -7.42 -1.25 5.08 -4.20 -1.29 -11.60
Bud Neck 2.03 1.92 0.65 -3.82 -4.30 -3.93 2.11 -8.85 -5.33 -9.46 -3.43 0.50 -9.62 -4.58 -10.37
Bud Site 2.12 2.13 1.94 -3.91 -4.23 -2.62 1.46 -5.25 -2.12 -5.91 -0.27 2.36 -1.76 -1.64 -7.22
Cell Periphery 1.12 1.14 0.85 -2.80 -3.01 -1.66 1.77 -3.88 -1.91 -5.33 0.23 1.70 -1.68 -2.46 -4.23
Cytoplasm 2.25 2.63 1.85 -1.44 -4.32 -2.20 1.49 -2.26 -0.76 -2.59 -1.72 2.74 -2.67 -0.55 -4.15
Cytoplasmic Foci 2.45 2.52 2.28 -2.26 -4.50 -2.09 1.13 -3.79 -1.85 -4.21 -0.35 2.93 -1.76 -1.23 -5.71
Eisosomes 1.82 -2.34 -0.54 -3.30 3.23 -2.15 -10.63 -2.55 0.10 4.24 -1.99 -10.92 -4.02 -0.46 5.52
Endoplasmic Reticulum 1.39 1.40 1.35 -3.38 -4.92 -1.04 5.75 -2.39 -0.55 -8.28 1.02 2.19 0.97 -0.24 -8.98
Endosome 2.67 2.67 2.58 -3.72 -3.91 -1.38 1.43 -4.05 -1.17 -4.21 0.33 2.65 -0.21 -0.55 -5.48
Golgi 2.07 2.11 1.71 -2.46 -2.78 0.36 1.61 -2.22 -3.05 -4.13 1.46 2.50 -1.25 -3.32 -4.49
Lipid Particles 1.61 1.63 1.49 -3.24 -4.03 -2.07 1.59 -3.49 1.63 -4.28 -1.88 2.10 -1.04 1.74 -5.77
Mitochondria 1.90 1.95 1.79 -3.84 -7.12 -2.24 5.16 -5.39 -0.50 -7.18 -0.62 2.75 -1.04 -0.10 -10.12
Mitotic Spindle -1.01 1.65 1.13 2.05 -4.58 -3.76 3.67 -1.93 2.54 -5.61 -4.02 4.00 -0.67 3.87 -7.25
None -0.77 0.80 -4.88 -3.91 -5.09 1.86 4.01 -1.95 -3.67 -5.22 1.72 3.41 -4.24 -5.82 -6.83
Nuclear Periphery -0.50 2.83 -1.25 -0.48 -6.11 0.55 3.50 -0.86 -1.73 -5.57 0.53 3.59 -0.34 -1.33 -8.13
Nuclear Periphery Foci 1.25 1.99 -0.29 -2.37 -3.70 -1.86 3.31 -1.07 -0.30 -1.82 -1.69 3.63 -1.40 -0.16 -3.09
Nucleolus -5.93 -1.21 -0.84 6.88 1.45 -0.47 7.50 10.67 10.94 5.31 -2.04 7.97 10.33 12.47 4.79
Nucleus 0.24 -3.92 -3.34 -6.41 4.54 3.66 -8.81 -3.88 -7.24 4.41 3.96 -10.13 -5.82 -9.94 6.21
Peroxisomes -1.57 1.59 0.80 2.50 -2.32 -2.82 3.27 1.98 4.07 -0.53 -3.19 3.24 1.27 4.15 -2.26
Vacuole 2.86 2.87 2.68 -4.96 -5.15 -1.72 2.37 -3.91 -2.94 -4.81 1.85 3.16 -0.31 -3.88 -7.02
Vacuole Periphery 2.23 2.24 1.93 -3.02 -3.12 -0.48 2.13 -2.27 -2.10 -3.78 1.52 2.91 -0.94 -2.92 -4.52
Standard name
Human Ortholog
Description RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rpb4

Rpb4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpb4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available