Standard name
Human Ortholog
Description Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0.05 0 0.06 0.07 0.11 0.05 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.1 0.07 – 0.26 0.17 0.3 0.14 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.92 0.95 0.98 0.95 0.95 0.93 – 0.92 0.86 0.83 0.89 0.93 0.89 0.86 0.89 0.89 0.91 0.93 0.92 0.93 0.88 0.74 0.68
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0.07 0.11 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0.05 0.14 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 0
Bud 0 0 0 0 0 0 0 – 0 3 1 0 0 0 0 0 0 0 1 2 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 1 0 0 0 0 0 1 – 5 2 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 5 9 6 3 12 5 11 – 4 6 11 8 16 24 35 20 14 10 0 2 0 0 1 1
Endoplasmic Reticulum 0 0 1 0 0 0 2 – 0 0 0 1 0 0 0 15 4 5 0 0 1 1 4 0
Endosome 1 0 0 0 1 0 1 – 0 1 0 0 0 0 1 4 2 3 1 1 1 0 1 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 3
Mitochondria 5 19 1 7 3 23 34 – 53 48 69 50 16 1 9 6 10 7 0 3 1 0 2 2
Nucleus 132 235 198 171 273 229 454 – 186 241 193 323 253 292 284 362 248 202 143 239 193 176 228 186
Nuclear Periphery 0 0 1 0 1 0 1 – 0 1 1 2 1 1 1 1 1 0 0 0 0 0 1 0
Nucleolus 1 2 0 1 0 2 13 – 14 31 33 29 2 7 6 2 1 1 0 0 0 3 15 18
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 0 0 – 1 0 0 0 0 2 0 1 0 0 0 0 0 1 1 0
Vac/Vac Membrane 4 7 3 0 3 2 5 – 2 2 2 3 0 5 6 27 16 8 3 4 7 10 41 47
Unique Cell Count 144 255 208 174 288 242 488 203 280 232 361 271 328 329 408 278 222 153 259 208 200 309 275
Labelled Cell Count 150 272 210 182 294 261 522 265 335 310 418 288 332 343 438 298 236 153 259 208 200 309 275


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 6.9 7.1 6.6 7.4 5.9 6.2 – 5.5 5.5 5.1 6.2 7.8 8.3 8.0 8.4 8.4 8.0 7.8 8.1 8.4
Std Deviation (1e-4) 2.0 1.4 1.3 1.1 1.5 1.4 1.4 – 1.2 1.3 1.2 1.4 2.1 2.3 2.2 1.9 2.0 1.8 2.1 2.3 1.9
Intensity Change (Log2) – – – -0.1 0.07 -0.28 -0.21 – -0.38 -0.36 -0.47 -0.21 0.13 0.22 0.17 0.24 0.24 0.17 0.14 0.19 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.8 -0.6 -0.5 – 0 -0.5 1.0 -0.5 1.6 2.2 3.3 1.2 1.2 0.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 2.4 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 3.6 – 7.7 6.1 8.4 5.4 3.2 0 0 0 0 0
Nucleus 1.7 -0.2 -0.3 -1.1 – -1.5 -3.3 -4.0 -2.4 -0.8 -2.5 -3.3 -2.6 -2.4 -1.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 3.9 5.0 5.7 4.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 2.8 2.4 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.597 5.7924 5.3358 4.7374 4.3003 5.0457 4.1564 4.3441 4.0499 3.4685 3.3449 3.6194 5.3163 9.3354 4.3262 3.5785 4.0343 9.4695
Actin 0.0144 0.0004 0.0058 0.037 0.0002 0 0.0002 0.0002 0.0005 0 0.0001 0 0.003 0 0.0041 0.0068 0.0008 0.0003
Bud 0.0005 0 0 0.0001 0 0 0.0002 0 0.0001 0 0.0003 0.0001 0.0005 0 0.0003 0.0069 0.0002 0
Bud Neck 0.0017 0 0.0001 0.0001 0.0003 0.0003 0.0003 0 0 0 0.0004 0.0008 0.0011 0 0.0001 0.0003 0.0007 0.0005
Bud Periphery 0.0012 0 0 0.0002 0 0 0.0004 0.0001 0.0003 0 0.0005 0.0001 0.0008 0 0.0003 0.0031 0.0005 0
Bud Site 0.0053 0.0001 0.0002 0.0005 0.0003 0 0.0005 0.0001 0.0002 0 0.001 0.0005 0.0017 0 0.001 0.0015 0.0002 0
Cell Periphery 0.0005 0 0 0 0 0 0.0002 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0001 0.0007 0.0001 0
Cytoplasm 0.0015 0.0001 0.0008 0.0001 0.0027 0 0.0011 0.0001 0.001 0 0.0006 0.0013 0.0067 0.0188 0.0144 0.0393 0.0003 0.0114
Cytoplasmic Foci 0.0093 0.0018 0.0019 0.0019 0.0022 0 0.0023 0 0.0002 0 0.0056 0.0006 0.0117 0.0001 0.0177 0.0216 0 0
Eisosomes 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0015 0 0.0006 0 0.0005 0 0.0009 0.0003 0.0005 0 0.0003 0.0001 0.0029 0.0009 0.0018 0.0064 0.0004 0.0003
Endosome 0.0049 0.0002 0.002 0.0006 0.0028 0 0.0065 0.0002 0.0015 0 0.004 0.0015 0.0061 0.0003 0.0124 0.0441 0.0004 0.0002
Golgi 0.0024 0.0004 0.0007 0.0026 0.0002 0 0.001 0 0.0001 0 0.0004 0.0001 0.0005 0 0.0067 0.0032 0 0
Lipid Particles 0.0091 0.0038 0.0029 0.004 0.0017 0 0.0129 0 0.0001 0 0.0083 0.0001 0.0038 0.0001 0.0054 0.0023 0 0
Mitochondria 0.0038 0.0002 0.0111 0.0007 0.0003 0.0001 0.0038 0.0009 0.0016 0 0.0015 0.0001 0.0023 0.0001 0.0053 0.0384 0.0022 0.0001
None 0.0039 0 0.0006 0.0001 0.0041 0 0.0003 0 0.0001 0 0.0003 0 0.0029 0 0.0034 0.0081 0.0001 0
Nuclear Periphery 0.0073 0.001 0.0024 0.0008 0.0063 0.0002 0.0047 0.0058 0.0048 0.0001 0.0022 0.0002 0.039 0.0797 0.0012 0.0153 0.0158 0.1306
Nucleolus 0.0186 0.0049 0.0038 0.0028 0.0145 0.0236 0.007 0.0061 0.0039 0.0034 0.0354 0.0181 0.0156 0.0035 0.0033 0.0303 0.0239 0.0121
Nucleus 0.886 0.9769 0.9631 0.9374 0.9292 0.9756 0.9465 0.9846 0.9824 0.9964 0.9306 0.9729 0.8854 0.8943 0.9154 0.751 0.9388 0.8419
Peroxisomes 0.0056 0.0098 0.0024 0.0103 0.0001 0 0.0016 0 0.0001 0 0.0007 0 0.0003 0 0.0048 0.0032 0 0
Punctate Nuclear 0.0164 0.0003 0.001 0.0005 0.0334 0.0001 0.0032 0.0005 0.0006 0 0.0035 0.0002 0.0126 0.0014 0.001 0.0089 0.0139 0.0011
Vacuole 0.0046 0.0001 0.0003 0 0.0007 0.0001 0.0045 0.0003 0.0013 0 0.0035 0.003 0.0016 0.0002 0.0007 0.0043 0.0006 0.0002
Vacuole Periphery 0.0013 0.0001 0.0001 0.0001 0.0004 0 0.0016 0.0006 0.0006 0 0.0008 0.0001 0.0013 0.0007 0.0005 0.0042 0.0009 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6904 11.4828 15.3374 21.9843 22.4834 14.9778 13.5453 15.8029 15.5436 27.6353
Translational Efficiency 0.6953 0.8305 0.6731 0.4576 0.6084 0.9048 0.7928 0.426 0.5627 0.4937

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2025 1287 778 1113 1700 1456 186 102 3725 2743 964 1215

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 760.67 938.74 1244.14 1141.73 835.71 918.21 1246.11 1195.66 794.92 927.84 1244.52 1146.26
Standard Deviation 97.26 156.78 191.92 171.00 128.08 151.62 156.66 211.65 118.43 154.40 185.64 175.42
Intensity Change Log 2 — 0.303455 0.709806 0.585879 — 0.135822 0.576357 0.516733 — 0.218131 0.641486 0.550094

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000101 0.000318 0.000880 0.001695 0.000217 0.000393 0.000869 0.001181 0.000154 0.000358 0.000878 0.001652
Bud Neck 0.001772 0.005424 0.017372 0.035005 0.003408 0.005886 0.008396 0.014139 0.002519 0.005669 0.015640 0.033254
Bud Site 0.000839 0.000805 0.001196 0.007366 0.000359 0.001160 0.000705 0.002203 0.000620 0.000994 0.001101 0.006933
Cell Periphery 0.000182 0.000182 0.000054 0.000196 0.000216 0.000111 0.000038 0.000199 0.000197 0.000144 0.000051 0.000196
Cytoplasm 0.006429 0.001634 0.000437 0.004512 0.002024 0.001324 0.000177 0.002503 0.004419 0.001470 0.000387 0.004343
Cytoplasmic Foci 0.000901 0.000320 0.000026 0.003089 0.000250 0.000555 0.000015 0.000049 0.000604 0.000445 0.000024 0.002834
Eisosomes 0.000015 0.000037 0.000052 0.000032 0.000023 0.000034 0.000055 0.000040 0.000019 0.000035 0.000053 0.000033
Endoplasmic Reticulum 0.003384 0.006612 0.002644 0.004125 0.006331 0.007393 0.003946 0.003319 0.004729 0.007026 0.002895 0.004057
Endosome 0.000393 0.000585 0.000077 0.004100 0.000189 0.000569 0.000065 0.000158 0.000300 0.000576 0.000075 0.003769
Golgi 0.000181 0.000573 0.000056 0.003675 0.000021 0.000247 0.000038 0.000101 0.000108 0.000400 0.000052 0.003375
Lipid Particles 0.000135 0.000156 0.000052 0.000415 0.000081 0.000295 0.000042 0.000070 0.000110 0.000230 0.000050 0.000386
Mitochondria 0.001054 0.001428 0.001631 0.005153 0.000382 0.001688 0.001122 0.001556 0.000747 0.001566 0.001533 0.004851
Mitotic Spindle 0.000664 0.002472 0.000167 0.022178 0.000424 0.002654 0.000031 0.000929 0.000555 0.002569 0.000141 0.020395
None 0.004447 0.001223 0.000666 0.003319 0.002306 0.002305 0.000578 0.003761 0.003470 0.001797 0.000649 0.003356
Nuclear Periphery 0.000317 0.000316 0.000211 0.002261 0.000301 0.000461 0.000178 0.000396 0.000310 0.000393 0.000204 0.002104
Nuclear Periphery Foci 0.000113 0.000451 0.000029 0.001526 0.000146 0.000500 0.000012 0.000096 0.000128 0.000477 0.000026 0.001406
Nucleolus 0.002972 0.003581 0.006929 0.005298 0.004665 0.008053 0.004155 0.004370 0.003745 0.005955 0.006394 0.005220
Nucleus 0.974497* 0.971818* 0.966025* 0.887113* 0.977854* 0.963107* 0.978075* 0.962631* 0.976029* 0.967194* 0.968350* 0.893453*
Peroxisomes 0.000306 0.000587 0.000465 0.000916 0.000093 0.000616 0.000360 0.000302 0.000209 0.000602 0.000444 0.000864
Vacuole 0.001024 0.000752 0.000926 0.004917 0.000609 0.002122 0.001074 0.001868 0.000835 0.001479 0.000954 0.004662
Vacuole Periphery 0.000273 0.000725 0.000106 0.003108 0.000102 0.000529 0.000069 0.000127 0.000195 0.000621 0.000099 0.002858

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.10 -12.93 -8.10 -7.06 -2.99 -3.99 -9.89 -5.58 -4.93 -2.79 -8.03 -14.21 -8.50 -7.42 -3.41
Bud Neck -6.22 -13.20 -17.50 -15.73 -9.09 -4.12 -6.40 -5.58 -4.76 -3.34 -7.38 -13.82 -17.80 -16.15 -9.99
Bud Site 0.10 -2.56 -5.74 -5.85 -5.09 -2.26 -4.21 -3.69 -2.34 -2.75 -1.59 -4.42 -6.23 -5.76 -5.34
Cell Periphery -0.01 4.42 0.67 0.59 -4.74 1.65 2.77 0.72 -1.01 -2.48 1.48 4.30 1.02 -0.63 -5.30
Cytoplasm 2.94 6.12 1.57 -1.72 -5.41 1.16 3.34 -0.39 -1.35 -2.96 3.40 6.89 0.13 -2.93 -5.67
Cytoplasmic Foci 1.95 2.88 -0.36 -1.97 -2.65 -0.93 2.02 1.74 1.98 -2.33 1.10 3.33 -0.98 -1.64 -2.66
Eisosomes -7.65 -17.09 -13.56 -1.62 6.23 -3.14 -9.79 -5.64 -2.99 0.88 -7.30 -18.50 -13.47 -2.74 6.53
Endoplasmic Reticulum -7.01 0.19 -4.58 2.91 -3.78 -1.90 4.75 3.10 4.58 -0.30 -6.30 3.64 -0.75 4.85 -3.80
Endosome -0.44 1.90 -2.88 -2.48 -3.71 -2.21 2.87 -0.80 1.96 -2.74 -1.38 2.27 -3.19 -2.59 -3.73
Golgi -0.86 0.73 -2.43 -1.53 -2.65 -1.85 -5.17 -5.92 0.52 -4.45 -1.30 0.44 -2.59 -1.97 -2.68
Lipid Particles -0.15 1.28 -2.28 -1.87 -3.09 -2.67 1.04 -1.34 1.54 -1.99 -2.04 1.20 -2.75 -1.16 -3.25
Mitochondria -0.57 -1.82 -5.28 -4.75 -5.49 -3.37 -8.21 -8.54 -1.82 -4.70 -2.08 -3.80 -7.47 -5.47 -5.96
Mitotic Spindle -1.57 0.88 -6.49 -5.65 -6.63 -1.89 0.11 -1.84 0.68 -2.11 -2.44 0.76 -6.56 -5.72 -6.66
None 4.38 5.23 1.22 -5.90 -8.11 -0.01 3.82 -0.83 -0.75 -2.69 3.28 6.42 0.00 -3.90 -8.49
Nuclear Periphery -0.12 0.45 -6.51 -6.48 -6.72 -2.21 0.22 -3.99 -2.40 -3.95 -1.56 0.53 -6.74 -6.39 -6.85
Nuclear Periphery Foci -1.12 2.03 -2.47 -1.21 -2.67 -1.65 1.19 -0.22 1.61 -2.08 -1.91 1.85 -2.44 -1.29 -2.70
Nucleolus -1.28 -5.36 -6.60 -6.17 -2.95 -3.35 -0.48 -1.96 0.52 -1.39 -3.81 -4.76 -6.31 -4.41 -3.32
Nucleus 0.93 4.53 14.76 13.85 12.63 4.59 1.89 4.88 2.49 4.03 3.80 5.82 15.79 13.75 13.27
Peroxisomes -0.72 -1.43 -1.29 -0.17 -0.57 -2.71 -6.56 -3.19 0.51 -1.08 -1.95 -3.50 -2.05 -0.12 -0.73
Vacuole 0.92 -1.66 -6.31 -6.92 -5.05 -2.58 -3.22 -1.50 -0.79 -1.23 -1.95 -2.37 -6.83 -5.56 -5.37
Vacuole Periphery -1.10 1.49 -1.89 -1.01 -2.15 -2.25 1.50 -2.43 1.94 -3.59 -1.97 1.43 -2.02 -1.19 -2.17
Standard name
Human Ortholog
Description Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Psy2

Psy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available