Standard name | |
---|---|
Human Ortholog | |
Description | Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis by phosphorylating Ldb19p; positively regulates activity of the three Mep ammonium transport proteins; mediates inhibitory phosphorylation of Mep2p and Par32p; TOR complex negatively regulates Npr1p activity; NPR1 has a paralog, PRR2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 7 | – | 5 | 4 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
Bud | 0 | 1 | 0 | 2 | 2 | 4 | 4 | – | 5 | 8 | 1 | 8 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 24 | 77 | 80 | 70 | 107 | – | 74 | 62 | 45 | 79 | 6 | 27 | 8 | 31 | 17 | 7 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 42 | 263 | 337 | 266 | 372 | 278 | 487 | – | 251 | 307 | 178 | 264 | 541 | 820 | 824 | 410 | 213 | 185 | 58 | 253 | 326 | 400 | 582 | 744 |
Endoplasmic Reticulum | 0 | 0 | 5 | 6 | 16 | 11 | 47 | – | 38 | 13 | 16 | 37 | 11 | 7 | 10 | 10 | 9 | 3 | 0 | 1 | 3 | 16 | 30 | 18 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 0 | 1 | 0 | 0 | 0 | 0 | 13 | – | 17 | 14 | 28 | 16 | 1 | 1 | 2 | 6 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 1 |
Nucleus | 1 | 0 | 2 | 3 | 0 | 2 | 3 | – | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 5 | 7 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 11 | 0 | 3 | 5 | 14 | 5 | 4 | 1 | 4 | 3 | 6 | 6 | 6 |
Unique Cell Count | 43 | 264 | 341 | 272 | 390 | 301 | 552 | 321 | 352 | 219 | 347 | 551 | 830 | 836 | 435 | 223 | 191 | 63 | 264 | 343 | 431 | 642 | 791 | |
Labelled Cell Count | 43 | 269 | 370 | 354 | 470 | 366 | 668 | 391 | 409 | 275 | 423 | 561 | 863 | 853 | 478 | 254 | 204 | 63 | 264 | 343 | 431 | 642 | 791 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.2 | 13.4 | 13.5 | 12.5 | 13.6 | 11.3 | 10.9 | – | 9.7 | 9.0 | 8.6 | 9.7 | 13.0 | 13.1 | 12.6 | 16.5 | 17.3 | 17.2 | 11.9 | 12.7 | 13.5 |
Std Deviation (1e-4) | 3.4 | 2.2 | 1.8 | 1.6 | 1.7 | 1.8 | 1.6 | – | 1.7 | 1.6 | 1.6 | 1.5 | 1.8 | 2.1 | 2.3 | 3.8 | 4.0 | 3.6 | 1.6 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.01 | -0.25 | -0.31 | – | -0.47 | -0.58 | -0.65 | -0.47 | -0.05 | -0.05 | -0.1 | 0.29 | 0.36 | 0.35 | -0.18 | -0.08 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 7.1 | 5.2 | 5.8 | 5.1 | – | 5.8 | 4.2 | 4.7 | 5.8 | -4.8 | -2.9 | -5.8 | 0 | 0.3 | -1.6 |
Cytoplasm | -1.0 | -2.7 | -4.1 | -5.8 | – | -8.4 | -6.0 | -7.5 | -9.0 | -0.7 | 0 | -0.4 | -3.3 | -2.5 | -1.6 |
Endoplasmic Reticulum | 0 | 2.1 | 1.8 | 4.4 | – | 5.4 | 1.8 | 3.5 | 5.0 | 0.6 | 0 | 0 | 0.8 | 1.9 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 4.3 | 3.7 | 6.8 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 2.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.5059 | 9.1961 | 8.5494 | 9.3463 | 8.2086 | 8.4733 | 6.003 | 8.5533 | 6.8453 | 6.6417 | 6.3201 | 7.4472 | 6.3073 | 9.7336 | 7.9364 | 7.2607 | 6.4825 | 8.4281 |
Actin | 0.0263 | 0.0105 | 0.0035 | 0.001 | 0.0214 | 0.0021 | 0.0152 | 0.0001 | 0.0095 | 0.0001 | 0.0296 | 0.0048 | 0.0258 | 0.0001 | 0.0018 | 0.001 | 0.0045 | 0.0002 |
Bud | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0392 | 0.0002 | 0.0008 | 0.0002 | 0.0009 | 0.0003 | 0.0021 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0022 | 0.0005 | 0 |
Bud Neck | 0.0098 | 0.0003 | 0.0004 | 0.0003 | 0.0018 | 0.001 | 0.0035 | 0.0002 | 0.0011 | 0.0002 | 0.0013 | 0.0006 | 0.001 | 0.0001 | 0.0002 | 0.001 | 0.001 | 0.0005 |
Bud Periphery | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0113 | 0.0002 | 0.0008 | 0 | 0.0006 | 0 | 0.0058 | 0.0003 | 0.0007 | 0 | 0.0001 | 0.0026 | 0.0004 | 0 |
Bud Site | 0.0023 | 0.0031 | 0.0005 | 0.0004 | 0.0019 | 0.002 | 0.0038 | 0.0015 | 0.0022 | 0.0001 | 0.0031 | 0.0002 | 0.0024 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0 |
Cell Periphery | 0.0008 | 0.001 | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.7852 | 0.9616 | 0.9287 | 0.9758 | 0.809 | 0.9366 | 0.7925 | 0.9874 | 0.8894 | 0.9382 | 0.8487 | 0.953 | 0.8268 | 0.9891 | 0.9507 | 0.9493 | 0.894 | 0.9764 |
Cytoplasmic Foci | 0.0311 | 0.0051 | 0.0069 | 0.0065 | 0.0234 | 0.0046 | 0.0275 | 0.0031 | 0.0221 | 0.0066 | 0.0074 | 0.0051 | 0.0208 | 0.0011 | 0.0061 | 0.0094 | 0.0196 | 0.003 |
Eisosomes | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0407 | 0.0031 | 0.0092 | 0.0027 | 0.0042 | 0.017 | 0.0121 | 0.0012 | 0.0038 | 0.0109 | 0.0046 | 0.0044 | 0.0197 | 0.0066 | 0.0075 | 0.0028 | 0.0022 | 0.0069 |
Endosome | 0.0235 | 0.0035 | 0.0031 | 0.0056 | 0.0285 | 0.0088 | 0.0644 | 0.0034 | 0.0283 | 0.0231 | 0.0189 | 0.0105 | 0.0296 | 0.0005 | 0.0144 | 0.0144 | 0.0337 | 0.0031 |
Golgi | 0.0068 | 0.0006 | 0.0013 | 0.0004 | 0.022 | 0.0021 | 0.0223 | 0.0002 | 0.0114 | 0.0007 | 0.0223 | 0.0055 | 0.0078 | 0.0001 | 0.0017 | 0.0014 | 0.0093 | 0.0004 |
Lipid Particles | 0.0083 | 0.0014 | 0.0033 | 0.0001 | 0.0006 | 0.0017 | 0.0082 | 0 | 0.0028 | 0.0003 | 0.0046 | 0.0017 | 0.0167 | 0 | 0.0011 | 0.0002 | 0.0047 | 0.0054 |
Mitochondria | 0.0023 | 0.0009 | 0.0011 | 0.0001 | 0.0051 | 0.0012 | 0.0208 | 0 | 0.0103 | 0.0002 | 0.0353 | 0.004 | 0.0039 | 0 | 0.0013 | 0.0042 | 0.0033 | 0.0011 |
None | 0.0176 | 0.0038 | 0.0016 | 0.002 | 0.0245 | 0.0111 | 0.0044 | 0.0011 | 0.0019 | 0.0018 | 0.0037 | 0.0009 | 0.012 | 0.0011 | 0.0019 | 0.0016 | 0.0026 | 0.0005 |
Nuclear Periphery | 0.0154 | 0.0003 | 0.0031 | 0.0006 | 0.0007 | 0.0021 | 0.0034 | 0.0001 | 0.0011 | 0.002 | 0.0011 | 0.0042 | 0.0078 | 0.0002 | 0.0062 | 0.0007 | 0.0031 | 0.0004 |
Nucleolus | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.001 | 0.0002 | 0 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0002 | 0.0005 | 0 |
Nucleus | 0.0042 | 0.0005 | 0.0134 | 0.0009 | 0.0011 | 0.0024 | 0.0031 | 0.0005 | 0.0032 | 0.0012 | 0.0014 | 0.0009 | 0.0031 | 0.0004 | 0.0022 | 0.0017 | 0.0017 | 0.0005 |
Peroxisomes | 0.0123 | 0.0013 | 0.0217 | 0.0001 | 0.0011 | 0.0004 | 0.0044 | 0 | 0.0044 | 0 | 0.0026 | 0.0016 | 0.0092 | 0 | 0.0002 | 0.0006 | 0.0032 | 0.0007 |
Punctate Nuclear | 0.0062 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.004 | 0.0021 | 0 | 0.002 | 0.0001 | 0.0004 | 0.0005 | 0.006 | 0 | 0.0003 | 0.0002 | 0.0105 | 0.0001 |
Vacuole | 0.0037 | 0.002 | 0.001 | 0.0026 | 0.0023 | 0.0011 | 0.0073 | 0.0009 | 0.0038 | 0.0126 | 0.0037 | 0.0009 | 0.0036 | 0.0003 | 0.0024 | 0.0049 | 0.0032 | 0.0005 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0026 | 0.0001 | 0.001 | 0.0016 | 0.0017 | 0.0005 | 0.0011 | 0 | 0.0011 | 0.0013 | 0.0008 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4845 | 10.8073 | 14.4968 | 16.9396 | 11.756 | 13.477 | 15.5504 | 20.1399 | 16.2135 | 19.9744 |
Translational Efficiency | 0.9707 | 1.3471 | 1.0548 | 0.8951 | 1.2583 | 1.4506 | 1.3694 | 1.0704 | 1.2808 | 1.0473 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1153 | 1299 | 1914 | 1651 | 1363 | 1882 | 1881 | 185 | 2516 | 3181 | 3795 | 1836 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1172.81 | 1269.04 | 1080.02 | 1097.98 | 1241.00 | 1296.24 | 1214.50 | 1233.18 | 1209.75 | 1285.13 | 1146.68 | 1111.60 |
Standard Deviation | 182.30 | 207.80 | 668.69 | 134.88 | 190.50 | 205.47 | 158.05 | 171.24 | 189.85 | 206.86 | 492.36 | 144.81 |
Intensity Change Log 2 | — | 0.113768 | -0.118911 | -0.095118 | — | 0.062830 | -0.031141 | -0.009120 | — | 0.087804 | -0.073120 | -0.050264 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000351 | 0.000179 | 0.000422 | 0.001109 | 0.000386 | 0.000375 | 0.000454 | 0.003725 | 0.000370 | 0.000295 | 0.000438 | 0.001373 |
Bud Neck | 0.000410 | 0.000375 | 0.002365 | 0.003293 | 0.000346 | 0.000708 | 0.001090 | 0.003638 | 0.000375 | 0.000572 | 0.001733 | 0.003328 |
Bud Site | 0.017970 | 0.007749 | 0.012551 | 0.030717 | 0.012207 | 0.015491 | 0.011407 | 0.055708 | 0.014848 | 0.012330 | 0.011984 | 0.033235 |
Cell Periphery | 0.000412 | 0.000141 | 0.000054 | 0.000077 | 0.000451 | 0.000207 | 0.000108 | 0.000328 | 0.000433 | 0.000180 | 0.000081 | 0.000102 |
Cytoplasm | 0.886682* | 0.896591* | 0.812326* | 0.649687* | 0.889094* | 0.864310* | 0.801252* | 0.578063* | 0.887989* | 0.877492* | 0.806837* | 0.642470* |
Cytoplasmic Foci | 0.059706 | 0.058281 | 0.032532 | 0.050867 | 0.059970 | 0.073474 | 0.057203 | 0.092307 | 0.059849 | 0.067270 | 0.044760 | 0.055043 |
Eisosomes | 0.000007 | 0.000004 | 0.000009 | 0.000015 | 0.000006 | 0.000010 | 0.000010 | 0.000040 | 0.000006 | 0.000007 | 0.000010 | 0.000018 |
Endoplasmic Reticulum | 0.002949 | 0.002243 | 0.000099 | 0.000411 | 0.004950 | 0.001184 | 0.000278 | 0.000434 | 0.004033 | 0.001617 | 0.000188 | 0.000413 |
Endosome | 0.002510 | 0.002518 | 0.002178 | 0.008146 | 0.002532 | 0.003155 | 0.002650 | 0.020870 | 0.002522 | 0.002895 | 0.002412 | 0.009428 |
Golgi | 0.005652 | 0.003533 | 0.000540 | 0.005261 | 0.007188 | 0.007116 | 0.000738 | 0.072632 | 0.006484 | 0.005653 | 0.000638 | 0.012049 |
Lipid Particles | 0.000816 | 0.001132 | 0.000447 | 0.000939 | 0.001533 | 0.001190 | 0.001006 | 0.001605 | 0.001205 | 0.001166 | 0.000724 | 0.001007 |
Mitochondria | 0.000743 | 0.000172 | 0.000435 | 0.001011 | 0.000337 | 0.000459 | 0.000231 | 0.008909 | 0.000523 | 0.000341 | 0.000334 | 0.001807 |
Mitotic Spindle | 0.000694 | 0.000680 | 0.004768 | 0.024197 | 0.001044 | 0.001116 | 0.001121 | 0.012382 | 0.000884 | 0.000938 | 0.002960 | 0.023007 |
None | 0.000958 | 0.001374 | 0.004555 | 0.000586 | 0.000807 | 0.001649 | 0.000996 | 0.001103 | 0.000876 | 0.001537 | 0.002791 | 0.000638 |
Nuclear Periphery | 0.000065 | 0.000030 | 0.000053 | 0.000155 | 0.000021 | 0.000010 | 0.000048 | 0.000097 | 0.000041 | 0.000018 | 0.000051 | 0.000149 |
Nuclear Periphery Foci | 0.001174 | 0.002170 | 0.000710 | 0.001619 | 0.001509 | 0.000723 | 0.002966 | 0.001838 | 0.001355 | 0.001314 | 0.001828 | 0.001641 |
Nucleolus | 0.000042 | 0.000078 | 0.000104 | 0.000291 | 0.000035 | 0.000137 | 0.000105 | 0.000046 | 0.000038 | 0.000113 | 0.000105 | 0.000267 |
Nucleus | 0.003091 | 0.006128 | 0.017048 | 0.015290 | 0.002978 | 0.004827 | 0.011912 | 0.007262 | 0.003030 | 0.005358 | 0.014503 | 0.014481 |
Peroxisomes | 0.000232 | 0.000187 | 0.001142 | 0.002258 | 0.000181 | 0.000555 | 0.001068 | 0.005670 | 0.000204 | 0.000405 | 0.001105 | 0.002602 |
Vacuole | 0.012103 | 0.014720 | 0.107034 | 0.200304* | 0.011084 | 0.021932 | 0.105086 | 0.127395 | 0.011551 | 0.018987 | 0.106068 | 0.192957 |
Vacuole Periphery | 0.003432 | 0.001713 | 0.000628 | 0.003766 | 0.003341 | 0.001372 | 0.000268 | 0.005947 | 0.003382 | 0.001511 | 0.000449 | 0.003986 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.34 | 0.01 | -1.22 | -2.20 | -1.16 | 0.14 | -0.02 | -2.77 | -2.77 | -2.68 | 1.55 | 0.01 | -1.84 | -2.26 | -1.73 |
Bud Neck | 0.64 | -7.89 | -7.05 | -7.13 | -0.75 | -7.37 | -15.30 | -5.10 | -4.10 | -2.81 | -5.88 | -11.53 | -7.89 | -7.02 | -2.53 |
Bud Site | 7.32 | 4.69 | -0.11 | -6.29 | -4.18 | -3.01 | 0.97 | -3.84 | -3.24 | -4.01 | 2.78 | 4.38 | -2.90 | -4.55 | -5.41 |
Cell Periphery | 9.03 | 12.43 | 12.14 | 9.98 | -1.94 | 5.70 | 8.35 | 5.02 | 0.57 | -2.80 | 9.37 | 13.58 | 13.62 | 14.17 | 0.60 |
Cytoplasm | -1.48 | 19.11 | 32.53 | 34.86 | 15.50 | 4.39 | 20.30 | 16.06 | 14.98 | 10.44 | 2.67 | 27.86 | 38.29 | 37.10 | 19.63 |
Cytoplasmic Foci | 0.33 | 8.31 | 5.01 | 4.62 | -4.12 | -3.79 | 0.84 | -1.25 | 0.87 | -1.72 | -2.91 | 6.48 | 5.49 | 8.39 | -0.17 |
Eisosomes | 5.65 | -5.17 | -7.31 | -13.06 | -3.55 | -4.53 | -8.76 | -7.12 | -5.64 | -5.37 | -1.61 | -9.76 | -10.68 | -8.91 | -5.28 |
Endoplasmic Reticulum | 0.76 | 3.33 | 3.17 | 4.84 | -3.51 | 4.08 | 5.31 | 5.27 | 3.58 | -0.59 | 3.66 | 6.23 | 6.10 | 6.05 | -1.98 |
Endosome | -0.08 | 1.65 | -2.94 | -3.03 | -4.47 | -1.94 | 0.15 | -2.38 | -2.02 | -2.41 | -1.45 | 1.47 | -3.82 | -3.12 | -4.62 |
Golgi | 4.34 | 13.86 | 4.99 | 2.05 | -2.87 | 0.14 | 17.96 | -1.35 | -1.36 | -2.27 | 1.81 | 22.71 | 3.02 | 2.02 | -3.45 |
Lipid Particles | -2.29 | 3.56 | -0.34 | 1.93 | -3.07 | 2.09 | 3.01 | -2.38 | -3.55 | -3.96 | 0.31 | 4.38 | 2.00 | 1.64 | -1.77 |
Mitochondria | 1.11 | 0.92 | 0.59 | -1.83 | -1.09 | -0.50 | 1.15 | -1.29 | -1.19 | -1.43 | 0.67 | 1.24 | -0.26 | -1.20 | -2.06 |
Mitotic Spindle | -0.12 | -2.86 | -5.99 | -5.99 | -4.15 | -0.15 | 0.05 | -2.14 | -2.13 | -2.15 | -0.22 | -2.23 | -6.23 | -6.22 | -5.42 |
None | -1.42 | -1.74 | 1.64 | 4.41 | 2.24 | -2.19 | 0.28 | 0.78 | 2.42 | 0.35 | -2.53 | -1.63 | 2.18 | 3.95 | 2.34 |
Nuclear Periphery | 0.63 | -0.70 | -2.12 | -10.31 | -6.81 | 1.53 | -6.64 | -6.24 | -6.75 | -4.65 | 0.90 | -1.86 | -5.33 | -15.15 | -9.18 |
Nuclear Periphery Foci | -3.75 | 3.21 | -1.78 | 2.72 | -4.82 | 5.54 | -6.64 | -4.17 | -7.46 | 0.71 | 0.19 | -4.18 | -1.57 | -1.58 | 2.27 |
Nucleolus | -1.95 | -10.19 | -3.50 | -2.56 | -2.12 | -1.74 | -11.29 | -2.10 | 1.53 | 7.05 | -2.09 | -15.41 | -3.60 | -1.35 | -2.09 |
Nucleus | -5.83 | -19.43 | -21.79 | -15.98 | 0.48 | -8.05 | -25.06 | -5.89 | -4.48 | 2.62 | -9.10 | -28.66 | -23.22 | -19.22 | -1.77 |
Peroxisomes | 1.94 | -3.59 | -3.12 | -3.26 | -1.04 | -7.95 | -1.74 | -2.26 | -1.85 | -1.47 | -6.89 | -3.05 | -3.78 | -3.03 | -1.32 |
Vacuole | -1.98 | -34.64 | -41.76 | -40.77 | -13.81 | -7.26 | -34.53 | -14.22 | -13.68 | -7.85 | -6.97 | -48.72 | -44.31 | -42.70 | -18.07 |
Vacuole Periphery | 1.69 | 3.81 | 2.03 | 0.21 | -2.07 | 3.07 | 4.96 | 1.57 | -0.06 | -1.19 | 3.31 | 6.14 | 2.71 | -0.02 | -2.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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