Standard name
Human Ortholog
Description Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.99 0.98 0.96 0.89 0.77 0.8 0.67 0.7 0.59 0.66 1.0 0.99 1.0 0.96 0.95 0.95 0.93 0.93 0.9 0.95 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0.13 0.1 0.32 0.49 0.36 0.57 0.58 0.67 0.62 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2 0 4 0 1 2
Bud 1 2 0 4 4 6 28 9 21 30 23 27 0 0 0 0 0 0 0 0 4 0 5 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 0 1 0 1 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 7 2 4 8 6 15 12 18 12 10 4 16 5 10 4 5 0 0 1 0 0 1
Cytoplasm 154 96 361 367 561 596 732 737 358 593 326 380 661 789 907 155 174 152 153 95 339 309 561 597
Endoplasmic Reticulum 2 0 2 0 2 1 0 1 2 1 2 1 2 3 4 2 7 5 0 0 4 0 2 1
Endosome 0 0 0 0 5 6 6 10 0 1 0 1 1 0 0 0 0 0 2 2 1 0 1 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 4 0 0 1 0 0 2 2
Mitochondria 20 2 15 48 58 217 462 329 305 488 368 357 0 0 1 1 3 5 2 0 9 3 5 5
Nucleus 0 0 0 0 0 1 1 3 1 1 2 1 1 0 1 4 2 4 0 0 0 0 2 2
Nuclear Periphery 0 0 0 0 0 1 0 2 0 0 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 1 0 1 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 5 1 2 2 0 0 0 0 1 0 0 0 1 1 3 7
Vac/Vac Membrane 0 0 1 0 3 6 11 20 8 20 13 16 0 1 1 3 4 3 0 2 3 2 2 1
Unique Cell Count 162 98 366 374 585 668 948 920 532 848 551 579 664 793 910 162 184 160 164 103 375 324 600 646
Labelled Cell Count 177 102 386 421 639 843 1248 1129 713 1154 753 802 669 812 919 176 200 174 164 103 375 324 600 646


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.0 4.5 4.2 4.5 4.0 3.9 4.0 3.8 3.7 3.6 3.6 6.0 6.0 6.0 12.8 13.2 12.0 6.1 6.0 6.0
Std Deviation (1e-4) 0.6 0.8 0.9 1.1 1.5 1.0 1.0 1.1 1.2 0.8 1.3 0.8 0.9 1.0 1.0 3.0 3.1 2.8 1.3 1.3 1.2
Intensity Change (Log2) – – – -0.11 -0.01 -0.19 -0.22 -0.15 -0.23 -0.27 -0.34 -0.32 0.41 0.4 0.41 1.51 1.55 1.42 0.43 0.43 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.1 0 2.6 0 0
Cytoplasm -0.5 -2.4 -5.5 -9.3 -8.5 -11.6 -11.2 -13.5 -12.0 1.6 1.6 2.1 -2.1 -2.8 -2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.3 0 0 0 -1.5 -0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.4 0 2.6 2.5 2.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6392 2.3182 2.0747 1.977 1.7656 2.2998 -1.356 -0.3887 -0.5813 -0.5505 -0.3135 -0.607 -1.6767 -0.4879 -0.9833 -0.8495 -0.9452 -0.8909
Actin 0.002 0.001 0.0011 0.0027 0.0013 0.0018 0.0171 0.0016 0.0193 0.001 0.0093 0.0027 0.005 0.0036 0.0112 0.0348 0.0004 0.0056
Bud 0.0016 0.0059 0.004 0.0062 0.0116 0.0053 0.001 0.0002 0.0019 0.0006 0.002 0.0003 0.0008 0.0013 0.0003 0.0002 0.0032 0.0004
Bud Neck 0.0011 0.0007 0.0005 0.0013 0.0005 0.0014 0.0011 0.0001 0.0063 0.0018 0.003 0.001 0.0013 0.0004 0.0006 0.0011 0.0031 0.0008
Bud Periphery 0.0019 0.0022 0.0025 0.0052 0.0219 0.0038 0.0017 0.0001 0.0025 0.0005 0.0022 0.0006 0.0011 0.0013 0.0003 0.0002 0.0021 0.0004
Bud Site 0.0083 0.0341 0.0102 0.0021 0.0022 0.0006 0.0174 0.0016 0.0112 0.0057 0.031 0.0003 0.006 0.0072 0.0011 0.0003 0.011 0.0008
Cell Periphery 0.0004 0.0005 0.0004 0.0006 0.0041 0.0001 0.0002 0.0002 0.0006 0.0004 0.0008 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.2264 0.3933 0.2993 0.2897 0.3746 0.401 0.3852 0.5922 0.4565 0.6569 0.2492 0.6736 0.2894 0.5866 0.5235 0.5454 0.5612 0.6982
Cytoplasmic Foci 0.014 0.016 0.0076 0.029 0.0121 0.0132 0.0539 0.0145 0.025 0.0455 0.0497 0.0263 0.0115 0.0208 0.0193 0.0175 0.0253 0.0082
Eisosomes 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0001
Endoplasmic Reticulum 0.0019 0.0008 0.0011 0.0056 0.0009 0.0014 0.006 0.0026 0.0036 0.0044 0.0024 0.0023 0.0042 0.0038 0.0025 0.0028 0.0026 0.0024
Endosome 0.0051 0.0028 0.0016 0.005 0.0027 0.0024 0.047 0.0058 0.0246 0.0287 0.1048 0.0267 0.0135 0.0044 0.0164 0.0144 0.0152 0.0052
Golgi 0.0009 0.0003 0.0002 0.0007 0.0003 0.0003 0.0177 0.0005 0.007 0.0114 0.1039 0.0072 0.0012 0.0005 0.0033 0.0042 0.002 0.0006
Lipid Particles 0.0009 0.0002 0.0002 0.0004 0.0002 0.0002 0.0219 0.0005 0.0049 0.0062 0.1026 0.0091 0.0013 0.0003 0.0068 0.0176 0.0004 0.0003
Mitochondria 0.0024 0.0003 0.0006 0.0008 0.0011 0.0006 0.0268 0.0001 0.0297 0.001 0.0751 0.0172 0.0013 0.0004 0.0018 0.0015 0.0005 0.0013
None 0.7141 0.5338 0.6647 0.6082 0.5506 0.5617 0.3789 0.3761 0.3728 0.2115 0.2085 0.2239 0.6425 0.3605 0.401 0.3431 0.3655 0.2679
Nuclear Periphery 0.0023 0.0004 0.0005 0.0025 0.0013 0.0005 0.0032 0.0006 0.0035 0.0016 0.0036 0.0006 0.0041 0.001 0.0011 0.0011 0.0006 0.0005
Nucleolus 0.0005 0.0003 0.0005 0.0016 0.0007 0.0004 0.0003 0.0001 0.0014 0.0011 0.0092 0.0001 0.0006 0.0002 0.0007 0.0001 0.0001 0.0008
Nucleus 0.0034 0.0015 0.0019 0.0119 0.0055 0.0018 0.0011 0.0006 0.002 0.0023 0.0096 0.0006 0.0075 0.0012 0.0011 0.0008 0.0011 0.0022
Peroxisomes 0.0077 0.0032 0.0004 0.0007 0.0003 0.0005 0.0106 0.0001 0.0071 0.0079 0.0119 0.0038 0.0006 0.0022 0.0018 0.0103 0.001 0.0016
Punctate Nuclear 0.0027 0.0013 0.0013 0.0236 0.0068 0.0021 0.0048 0.0005 0.0055 0.0019 0.007 0.0005 0.002 0.0015 0.0029 0.0013 0.0006 0.0006
Vacuole 0.0017 0.0011 0.0009 0.002 0.0009 0.0007 0.0028 0.0015 0.012 0.0089 0.01 0.0022 0.0049 0.0023 0.0033 0.0025 0.0035 0.0018
Vacuole Periphery 0.0003 0.0001 0.0002 0.0004 0.0002 0.0001 0.0012 0.0002 0.0025 0.0006 0.004 0.0009 0.0008 0.0002 0.0007 0.0005 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7229 10.3557 7.6843 8.1525 8.7614 5.7889 11.9976 13.8394 9.5296 10.4186
Translational Efficiency 0.8626 1.0014 0.8023 0.6932 0.697 1.3284 0.9003 0.7421 0.9002 0.6251

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
951 229 1227 284 1456 1049 235 201 2407 1278 1462 485

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.00 736.22 812.21 877.61 706.48 741.32 947.76 909.75 668.36 740.41 834.00 890.93
Standard Deviation 93.53 97.78 108.95 125.66 75.58 90.89 99.54 134.30 95.59 92.18 118.46 130.28
Intensity Change Log 2 — 0.271328 0.413044 0.524771 — 0.069448 0.423873 0.364821 — 0.166512 0.418865 0.441145

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000123 0.000756 0.000161 0.000632 0.000071 0.000407 0.000268 0.000262 0.000092 0.000470 0.000178 0.000478
Bud Neck 0.015527 0.022253 0.004490 0.004110 0.006103 0.026232 0.001998 0.017816 0.009827 0.025519 0.004089 0.009791
Bud Site 0.005175 0.029897 0.020067 0.026877 0.002656 0.014485 0.004146 0.027948 0.003651 0.017247 0.017508 0.027321
Cell Periphery 0.000142 0.000216 0.000096 0.000160 0.000235 0.000179 0.000258 0.000146 0.000198 0.000186 0.000122 0.000155
Cytoplasm 0.516951* 0.394145* 0.626394* 0.443999* 0.576763* 0.458381* 0.447181* 0.506779* 0.553131* 0.446870* 0.597587* 0.470017*
Cytoplasmic Foci 0.246451* 0.304249* 0.034868 0.086083 0.196845* 0.242901* 0.060563 0.036825 0.216444* 0.253894* 0.038998 0.065669
Eisosomes 0.000211 0.000180 0.000032 0.000076 0.000160 0.000145 0.000114 0.000060 0.000180 0.000151 0.000045 0.000069
Endoplasmic Reticulum 0.001116 0.000635 0.000962 0.004638 0.001854 0.000763 0.003356 0.000954 0.001562 0.000740 0.001347 0.003111
Endosome 0.021606 0.051855 0.004695 0.012172 0.008800 0.034606 0.008346 0.013580 0.013860 0.037697 0.005282 0.012755
Golgi 0.003660 0.006641 0.000440 0.003472 0.001765 0.006627 0.000108 0.000775 0.002514 0.006630 0.000387 0.002354
Lipid Particles 0.009165 0.004979 0.000930 0.002899 0.006998 0.005989 0.003403 0.001062 0.007854 0.005808 0.001327 0.002138
Mitochondria 0.004312 0.003097 0.000419 0.000843 0.002450 0.008532 0.000114 0.002160 0.003186 0.007558 0.000370 0.001389
Mitotic Spindle 0.000185 0.002074 0.012212 0.020409 0.000532 0.004943 0.000424 0.022333 0.000395 0.004429 0.010317 0.021206
None 0.007010 0.001843 0.001748 0.001307 0.002810 0.001354 0.001198 0.002394 0.004469 0.001442 0.001659 0.001758
Nuclear Periphery 0.000898 0.000293 0.000819 0.000628 0.000422 0.000277 0.001013 0.000525 0.000610 0.000280 0.000850 0.000585
Nuclear Periphery Foci 0.000542 0.000726 0.000756 0.002584 0.000376 0.000296 0.004880 0.002089 0.000441 0.000373 0.001419 0.002379
Nucleolus 0.001568 0.001491 0.000316 0.000223 0.000451 0.000807 0.000293 0.000202 0.000893 0.000930 0.000312 0.000215
Nucleus 0.089322 0.042333 0.126900 0.059036 0.125206 0.062353 0.146899* 0.057081 0.111028 0.058765 0.130115* 0.058226
Peroxisomes 0.005560 0.019427 0.000597 0.002539 0.001326 0.006336 0.000416 0.000793 0.002999 0.008682 0.000568 0.001815
Vacuole 0.069058 0.110610 0.162398 0.326588* 0.063131 0.120437 0.314851* 0.304830* 0.065473 0.118676 0.186903 0.317570*
Vacuole Periphery 0.001420 0.002299 0.000702 0.000725 0.001046 0.003948 0.000171 0.001385 0.001194 0.003653 0.000617 0.000999

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.54 -1.40 -3.56 1.28 -3.24 -2.27 -7.90 -5.98 0.55 -0.29 -3.05 -4.70 -5.11 0.19 -3.73
Bud Neck -1.97 6.32 7.04 6.60 1.23 -9.24 4.66 -0.40 4.12 -1.27 -8.09 6.18 2.46 8.27 -0.66
Bud Site -5.14 -4.42 -2.88 1.94 -0.45 -8.48 -4.66 -4.14 -1.29 -3.57 -9.38 -5.35 -4.93 -0.40 -1.41
Cell Periphery -1.93 6.57 1.81 2.51 -2.37 1.99 0.83 4.71 2.03 3.52 0.60 7.17 5.25 3.06 -1.51
Cytoplasm 6.26 -4.25 8.84 1.81 11.59 11.33 11.30 7.30 1.80 -1.88 11.58 1.97 12.37 4.59 10.31
Cytoplasmic Foci -3.42 31.95 24.57 16.81 -3.14 -5.83 24.29 28.68 29.43 2.45 -5.22 44.02 35.25 32.09 -2.14
Eisosomes 0.65 4.09 3.30 4.76 -8.00 1.86 4.94 13.19 10.91 5.13 1.58 7.63 6.45 12.01 -5.63
Endoplasmic Reticulum 1.88 -0.53 -1.70 -2.26 -1.61 2.94 -3.68 2.18 -1.92 6.93 3.27 -0.34 -0.80 -2.35 -0.70
Endosome -4.27 10.33 7.62 6.64 -1.41 -12.60 1.79 1.85 10.91 1.10 -10.92 11.73 6.54 13.95 -1.04
Golgi -1.14 6.70 3.15 2.16 -1.12 -5.30 6.11 4.96 7.16 -1.46 -4.67 8.12 3.79 6.32 -1.16
Lipid Particles 3.64 8.91 6.99 3.41 -3.13 1.34 6.60 11.97 8.13 5.87 3.01 13.83 11.73 7.30 -1.73
Mitochondria 0.78 3.23 3.21 2.39 0.10 -3.46 2.81 1.75 4.77 -1.64 -2.98 4.10 3.65 5.32 -1.02
Mitotic Spindle -1.41 -4.85 -3.02 -2.47 -0.63 -3.34 -2.06 -2.44 -1.34 -1.13 -3.70 -5.05 -3.85 -2.42 -0.77
None 2.92 3.21 3.36 0.60 0.84 1.85 1.19 0.41 -1.64 -0.86 3.60 3.48 3.20 -0.95 -0.43
Nuclear Periphery 1.85 -1.11 -0.76 -5.71 0.68 2.49 -8.34 -3.16 -4.78 5.36 2.51 -4.92 -2.37 -7.66 3.27
Nuclear Periphery Foci -1.50 -3.02 -4.04 -3.39 -3.37 1.08 -10.12 -4.41 -4.54 4.13 0.97 -9.86 -6.04 -6.14 -2.42
Nucleolus 0.19 5.41 6.27 2.74 1.53 -2.31 0.26 8.23 4.29 2.14 -0.20 5.04 7.99 5.00 2.26
Nucleus 7.16 -8.96 2.11 -3.57 9.01 12.91 -2.00 5.75 -1.68 5.40 13.47 -6.81 6.40 -2.74 10.88
Peroxisomes -3.61 5.48 4.69 4.96 -1.96 -7.44 6.83 4.83 8.80 -0.68 -6.03 6.45 5.34 8.98 -1.70
Vacuole -4.55 -19.34 -21.56 -16.84 -13.50 -11.05 -18.04 -15.84 -13.06 -0.61 -11.90 -25.68 -26.73 -22.24 -13.43
Vacuole Periphery -1.27 4.01 5.20 3.00 0.96 -6.31 5.83 2.04 7.08 -1.57 -6.28 4.17 4.85 8.26 0.39
Standard name
Human Ortholog
Description Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (46%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Glg2

Glg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available