Standard name | |
---|---|
Human Ortholog | |
Description | Essential component of GPI-mannosyltransferase II; complex is responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interactions with secretory pathway genes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 3 | 2 | 2 | 0 | 0 | 1 | 3 | 4 | 1 | 2 | 4 | 0 | 1 | 10 | 1 | 2 | 7 |
Bud | 0 | 0 | 0 | 2 | 3 | 5 | 9 | 18 | 7 | 10 | 13 | 6 | 0 | 0 | 2 | 0 | 2 | 0 | 4 | 2 | 6 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 2 | 7 | 11 | 8 | 13 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 2 | 2 | 0 | 3 | 2 | 1 | 1 | 0 | 1 | 2 | 11 | 18 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 9 | 24 | 20 | 17 | 30 | 28 | 32 | 18 | 16 | 18 | 14 | 4 | 21 | 24 | 14 | 7 | 5 | 0 | 1 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 5 | 6 | 9 | 1 | 2 | 12 | 13 | 10 | 9 | 2 | 4 | 3 | 19 | 20 | 16 | 7 | 14 | 7 | 12 | 27 | 119 | 35 | 59 | 62 |
Endosome | 0 | 0 | 16 | 19 | 21 | 22 | 33 | 52 | 23 | 31 | 30 | 32 | 1 | 2 | 3 | 7 | 9 | 4 | 2 | 4 | 11 | 5 | 15 | 26 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 | 11 | 12 | 0 | 2 | 5 | 1 | 8 | 12 |
Mitochondria | 2 | 6 | 26 | 16 | 32 | 157 | 207 | 199 | 245 | 155 | 242 | 234 | 1 | 0 | 1 | 9 | 12 | 3 | 3 | 5 | 11 | 6 | 16 | 17 |
Nucleus | 11 | 27 | 9 | 38 | 14 | 33 | 35 | 61 | 46 | 57 | 49 | 42 | 3 | 4 | 4 | 4 | 5 | 6 | 4 | 6 | 2 | 0 | 0 | 3 |
Nuclear Periphery | 70 | 145 | 303 | 96 | 153 | 192 | 183 | 200 | 90 | 100 | 84 | 144 | 40 | 44 | 52 | 22 | 44 | 40 | 156 | 221 | 343 | 87 | 92 | 84 |
Nucleolus | 2 | 2 | 24 | 6 | 16 | 32 | 48 | 67 | 38 | 32 | 40 | 48 | 1 | 1 | 1 | 4 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 0 | 3 | 0 | 4 | 1 | 7 | 2 | 2 | 1 | 6 | 1 | 3 | 4 | 0 | 2 | 2 | 1 | 1 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 112 | 143 | 139 | 89 | 145 | 146 | 126 | 169 | 105 | 126 | 98 | 107 | 11 | 36 | 51 | 73 | 89 | 77 | 20 | 39 | 22 | 12 | 23 | 27 |
Unique Cell Count | 168 | 267 | 461 | 251 | 350 | 512 | 557 | 671 | 492 | 443 | 461 | 508 | 63 | 120 | 141 | 125 | 155 | 132 | 210 | 317 | 547 | 153 | 228 | 252 |
Labelled Cell Count | 207 | 342 | 553 | 293 | 407 | 640 | 689 | 822 | 594 | 545 | 588 | 651 | 84 | 148 | 182 | 146 | 199 | 165 | 210 | 317 | 547 | 153 | 228 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 10.7 | 10.6 | 11.2 | 9.4 | 8.0 | 7.8 | 7.9 | 7.4 | 7.6 | 7.3 | 7.1 | 9.9 | 10.3 | 10.1 | 11.9 | 11.9 | 11.9 | 10.0 | 10.0 | 10.6 |
Std Deviation (1e-4) | 2.1 | 1.8 | 1.8 | 6.4 | 1.8 | 1.7 | 1.7 | 1.6 | 1.6 | 1.6 | 1.6 | 1.4 | 1.9 | 1.8 | 1.9 | 2.0 | 2.3 | 2.1 | 1.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.07 | -0.18 | -0.41 | -0.46 | -0.42 | -0.53 | -0.48 | -0.54 | -0.58 | -0.1 | -0.05 | -0.07 | 0.17 | 0.17 | 0.16 | -0.08 | -0.08 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | -0.2 | 0.4 | -0.1 | -0.3 | -1.2 | -1.2 | -0.9 | -2.0 | 0 | 4.5 | 4.5 | 2.4 | -0.3 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0.4 | 0.4 | -0.6 | -0.1 | -2.1 | -1.4 | -1.9 | 0 | 6.6 | 4.9 | 0 | 4.0 | 0 |
Endosome | 2.4 | 1.7 | 0.7 | 1.8 | 3.0 | 0.9 | 2.4 | 2.1 | 2.0 | 0 | 0 | 0 | 0 | 1.3 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.4 | 1.9 | 10.0 | 11.9 | 9.9 | 15.1 | 11.0 | 15.7 | 14.2 | 0 | -2.7 | -2.5 | 0.7 | 0.9 | -1.6 |
Nucleus | 6.8 | 1.7 | 3.4 | 3.4 | 4.9 | 4.9 | 6.3 | 5.4 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -7.1 | -6.3 | -8.8 | -10.5 | -11.9 | -14.9 | -13.0 | -14.6 | -11.7 | -0.3 | -5.8 | -6.1 | -9.6 | -8.1 | -7.3 |
Nucleolus | -1.8 | -0.4 | 0.7 | 2.1 | 2.9 | 1.6 | 1.3 | 2.1 | 2.5 | 0 | -2.1 | -2.3 | -0.9 | -2.9 | -1.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.4 | 3.3 | -0.6 | -2.7 | -1.8 | -3.1 | -0.6 | -3.1 | -3.2 | -2.1 | 0 | 1.3 | 5.8 | 6.1 | 5.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6996 | 4.9902 | 5.0201 | 4.4958 | 4.0819 | 4.8166 | 7.6798 | 7.3574 | 7.4799 | 7.0559 | 6.8197 | 8.4062 | 6.5923 | 6.5947 | 6.0509 | 5.4201 | 5.7359 | 5.8668 |
Actin | 0.0103 | 0.0009 | 0.0008 | 0.001 | 0.0036 | 0.0009 | 0.0097 | 0.0005 | 0.0092 | 0.0005 | 0.0041 | 0.0041 | 0.0021 | 0.0011 | 0.0004 | 0.0019 | 0.0013 | 0.0047 |
Bud | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 |
Bud Neck | 0.0001 | 0.0005 | 0.0001 | 0.011 | 0.0005 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.001 | 0.0028 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0006 |
Bud Site | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0 | 0 | 0.0022 | 0.0004 | 0.0012 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0009 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.002 | 0.0311 | 0.0056 | 0.0035 | 0.0008 | 0.0021 | 0.0097 | 0.0278 | 0.0011 | 0.0133 | 0.0021 | 0.0077 | 0.0012 | 0.0023 | 0.0013 | 0.0018 | 0.0027 | 0.0018 |
Cytoplasmic Foci | 0.0001 | 0.0021 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0053 | 0.0017 | 0.0038 | 0.0001 | 0.005 | 0.0004 | 0.0081 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0027 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0569 | 0.1874 | 0.1549 | 0.129 | 0.0624 | 0.103 | 0.0563 | 0.1202 | 0.1609 | 0.1785 | 0.072 | 0.0945 | 0.0505 | 0.2011 | 0.1786 | 0.2049 | 0.0964 | 0.1743 |
Endosome | 0.004 | 0.0168 | 0.0051 | 0.01 | 0.0083 | 0.0072 | 0.0256 | 0.0125 | 0.0116 | 0.0026 | 0.0241 | 0.0058 | 0.0048 | 0.0041 | 0.0168 | 0.0031 | 0.0045 | 0.014 |
Golgi | 0.0004 | 0.0008 | 0.0003 | 0.0007 | 0.0014 | 0.0004 | 0.005 | 0.0008 | 0.0033 | 0.0002 | 0.0085 | 0.0032 | 0.0009 | 0.0004 | 0.0098 | 0.0003 | 0.0001 | 0.0048 |
Lipid Particles | 0.001 | 0.0102 | 0.0009 | 0.0009 | 0.0025 | 0.0012 | 0.0168 | 0.0027 | 0.0108 | 0.0006 | 0.0098 | 0.0041 | 0.0159 | 0.0015 | 0.0004 | 0.0004 | 0.0003 | 0.0083 |
Mitochondria | 0.0011 | 0.0005 | 0.0008 | 0.0011 | 0.0009 | 0.0008 | 0.003 | 0.0005 | 0.0034 | 0.0006 | 0.0032 | 0.003 | 0.0011 | 0.0007 | 0.0005 | 0.0014 | 0.0005 | 0.0035 |
None | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0011 | 0.0006 | 0.0004 | 0.0002 | 0.0031 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.8373 | 0.6458 | 0.748 | 0.7606 | 0.8756 | 0.8203 | 0.7894 | 0.6831 | 0.7043 | 0.7168 | 0.7527 | 0.8171 | 0.854 | 0.6729 | 0.7441 | 0.7175 | 0.8014 | 0.7357 |
Nucleolus | 0.0002 | 0.001 | 0.0004 | 0.0008 | 0.0003 | 0.0002 | 0.0004 | 0.0022 | 0.0002 | 0.0001 | 0.0013 | 0.0003 | 0.0016 | 0.0013 | 0 | 0.0001 | 0.0003 | 0.0008 |
Nucleus | 0.0552 | 0.0596 | 0.0531 | 0.0491 | 0.0207 | 0.0419 | 0.0394 | 0.1121 | 0.0574 | 0.064 | 0.0668 | 0.0394 | 0.0258 | 0.0644 | 0.0225 | 0.0383 | 0.0799 | 0.0158 |
Peroxisomes | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0051 | 0.0002 | 0.0018 | 0 | 0.0013 | 0.0003 | 0.0007 | 0 | 0 | 0 | 0 | 0.0014 |
Punctate Nuclear | 0.0008 | 0.0066 | 0.001 | 0.0026 | 0.0015 | 0.001 | 0.0031 | 0.0055 | 0.0008 | 0.0005 | 0.0249 | 0.0011 | 0.0063 | 0.0011 | 0.0003 | 0.0004 | 0.0018 | 0.0021 |
Vacuole | 0.0051 | 0.0165 | 0.005 | 0.0063 | 0.0027 | 0.0053 | 0.0061 | 0.0106 | 0.0055 | 0.0024 | 0.0031 | 0.004 | 0.0025 | 0.0176 | 0.0036 | 0.0033 | 0.003 | 0.0053 |
Vacuole Periphery | 0.0245 | 0.0182 | 0.0234 | 0.0218 | 0.018 | 0.0148 | 0.0206 | 0.0177 | 0.0233 | 0.019 | 0.0167 | 0.0138 | 0.0231 | 0.0294 | 0.0206 | 0.0258 | 0.0064 | 0.0198 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6342 | 9.9133 | 13.4862 | 20.6731 | 16.7743 | 9.2361 | 17.9585 | 23.1141 | 18.8337 | 22.8951 |
Translational Efficiency | 1.1435 | 1.3792 | 1.0486 | 0.5221 | 0.8369 | 2.3707 | 1.1807 | 0.8621 | 0.9973 | 1.0015 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1414 | 746 | 689 | 1472 | 2740 | 1590 | 1632 | 2287 | 4154 | 2336 | 2321 | 3759 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 978.27 | 1187.03 | 1332.87 | 1350.30 | 1067.56 | 1070.80 | 1251.98 | 1428.11 | 1037.17 | 1107.92 | 1275.99 | 1397.64 |
Standard Deviation | 140.58 | 208.66 | 183.15 | 205.57 | 155.05 | 171.45 | 254.97 | 215.12 | 156.12 | 191.96 | 238.82 | 214.81 |
Intensity Change Log 2 | — | 0.279052 | 0.446231 | 0.464975 | — | 0.004372 | 0.229894 | 0.419790 | — | 0.142251 | 0.337394 | 0.441573 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000181 | 0.000335 | 0.000212 | 0.000502 | 0.000123 | 0.000272 | 0.000577 | 0.000215 | 0.000143 | 0.000292 | 0.000469 | 0.000327 |
Bud Neck | 0.000163 | 0.000496 | 0.001635 | 0.013616 | 0.000135 | 0.000416 | 0.003373 | 0.004484 | 0.000145 | 0.000441 | 0.002857 | 0.008060 |
Bud Site | 0.000117 | 0.001163 | 0.000318 | 0.008176 | 0.000244 | 0.000590 | 0.002061 | 0.003277 | 0.000201 | 0.000773 | 0.001543 | 0.005195 |
Cell Periphery | 0.000070 | 0.000118 | 0.000095 | 0.000398 | 0.000168 | 0.000141 | 0.000292 | 0.000250 | 0.000135 | 0.000133 | 0.000233 | 0.000308 |
Cytoplasm | 0.001525 | 0.005119 | 0.001643 | 0.023012 | 0.004472 | 0.004406 | 0.026324 | 0.015848 | 0.003469 | 0.004633 | 0.018997 | 0.018653 |
Cytoplasmic Foci | 0.000778 | 0.000892 | 0.000265 | 0.006261 | 0.000472 | 0.002695 | 0.001211 | 0.002312 | 0.000576 | 0.002119 | 0.000930 | 0.003859 |
Eisosomes | 0.000003 | 0.000006 | 0.000002 | 0.000014 | 0.000002 | 0.000005 | 0.000007 | 0.000014 | 0.000003 | 0.000005 | 0.000006 | 0.000014 |
Endoplasmic Reticulum | 0.063051 | 0.119448* | 0.109653* | 0.172685* | 0.150658* | 0.130955* | 0.163829* | 0.198318* | 0.120837* | 0.127280* | 0.147746* | 0.188280* |
Endosome | 0.005205 | 0.012914 | 0.008710 | 0.027048 | 0.006841 | 0.012442 | 0.013986 | 0.021068 | 0.006284 | 0.012592 | 0.012420 | 0.023410 |
Golgi | 0.000936 | 0.000618 | 0.000099 | 0.002158 | 0.000580 | 0.002105 | 0.000576 | 0.001665 | 0.000701 | 0.001630 | 0.000434 | 0.001858 |
Lipid Particles | 0.007359 | 0.004870 | 0.001485 | 0.008751 | 0.005947 | 0.009932 | 0.002099 | 0.004806 | 0.006427 | 0.008315 | 0.001917 | 0.006351 |
Mitochondria | 0.002789 | 0.001729 | 0.001358 | 0.003280 | 0.002751 | 0.004565 | 0.002668 | 0.002487 | 0.002764 | 0.003660 | 0.002279 | 0.002797 |
Mitotic Spindle | 0.000696 | 0.005217 | 0.004294 | 0.069490 | 0.000655 | 0.005323 | 0.006455 | 0.023925 | 0.000669 | 0.005289 | 0.005813 | 0.041768 |
None | 0.000841 | 0.000814 | 0.000240 | 0.002112 | 0.002532 | 0.001354 | 0.003845 | 0.001868 | 0.001956 | 0.001181 | 0.002775 | 0.001964 |
Nuclear Periphery | 0.366239* | 0.322225* | 0.514917* | 0.204741* | 0.374523* | 0.329126* | 0.388007* | 0.272761* | 0.371703* | 0.326922* | 0.425681* | 0.246125* |
Nuclear Periphery Foci | 0.535907* | 0.511845* | 0.346694* | 0.393583* | 0.442253* | 0.480190* | 0.325903* | 0.413741* | 0.474132* | 0.490299* | 0.332075* | 0.405847* |
Nucleolus | 0.002564 | 0.001177 | 0.000419 | 0.008479 | 0.000575 | 0.000608 | 0.003403 | 0.002128 | 0.001252 | 0.000789 | 0.002517 | 0.004615 |
Nucleus | 0.006010 | 0.004891 | 0.004425 | 0.027189 | 0.003409 | 0.004068 | 0.044017 | 0.007564 | 0.004294 | 0.004331 | 0.032264 | 0.015249 |
Peroxisomes | 0.000197 | 0.000446 | 0.000051 | 0.000455 | 0.000084 | 0.000409 | 0.000132 | 0.000094 | 0.000123 | 0.000421 | 0.000108 | 0.000236 |
Vacuole | 0.002758 | 0.004299 | 0.002998 | 0.023461 | 0.002190 | 0.006882 | 0.009823 | 0.018783 | 0.002384 | 0.006057 | 0.007797 | 0.020615 |
Vacuole Periphery | 0.002611 | 0.001379 | 0.000486 | 0.004589 | 0.001385 | 0.003518 | 0.001415 | 0.004392 | 0.001803 | 0.002835 | 0.001139 | 0.004469 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.60 | -1.10 | -6.60 | -2.92 | -4.23 | -3.45 | -7.08 | -3.67 | 0.32 | 5.39 | -3.46 | -6.88 | -7.54 | -2.70 | 1.91 |
Bud Neck | -3.30 | -3.78 | -12.57 | -12.28 | -10.59 | -2.58 | -7.87 | -10.46 | -9.65 | -2.05 | -3.71 | -8.71 | -15.89 | -15.26 | -9.30 |
Bud Site | -1.42 | -2.30 | -9.02 | -6.88 | -8.67 | -2.04 | -6.04 | -5.72 | -5.06 | -2.05 | -2.17 | -6.37 | -10.55 | -8.84 | -7.33 |
Cell Periphery | -3.23 | -2.42 | -8.77 | -7.33 | -7.68 | 0.65 | -3.51 | -2.63 | -3.82 | 1.33 | -0.03 | -4.08 | -6.99 | -7.50 | -2.98 |
Cytoplasm | -3.30 | -0.64 | -11.34 | -8.87 | -11.17 | 0.00 | -9.80 | -8.42 | -8.05 | 3.57 | -1.83 | -9.81 | -13.71 | -12.03 | -0.81 |
Cytoplasmic Foci | -0.55 | 1.99 | -7.37 | -6.40 | -8.18 | -4.04 | -4.01 | -6.80 | 0.02 | -3.77 | -3.87 | -2.51 | -9.77 | -4.30 | -8.56 |
Eisosomes | -1.49 | 0.49 | -9.84 | -5.64 | -9.33 | -3.02 | -6.57 | -1.34 | -1.11 | -0.64 | -3.09 | -5.92 | -2.36 | -1.96 | -1.57 |
Endoplasmic Reticulum | -7.58 | -6.29 | -13.17 | -4.69 | -5.70 | 2.93 | -1.59 | -6.22 | -8.42 | -4.17 | -1.25 | -4.69 | -11.44 | -9.34 | -5.84 |
Endosome | -6.63 | -4.52 | -15.63 | -8.41 | -12.50 | -5.49 | -9.16 | -14.12 | -6.59 | -6.01 | -8.02 | -10.30 | -20.80 | -10.31 | -11.65 |
Golgi | 0.85 | 2.39 | -2.36 | -3.89 | -6.00 | -2.64 | -0.23 | -3.19 | 0.71 | -3.67 | -2.21 | 1.18 | -4.06 | -0.55 | -6.26 |
Lipid Particles | 2.04 | 6.64 | -1.65 | -3.69 | -8.50 | -3.44 | 5.73 | 1.26 | 4.55 | -5.58 | -2.23 | 8.41 | -0.46 | 1.94 | -9.85 |
Mitochondria | 1.48 | 1.58 | -0.92 | -3.10 | -2.94 | -2.12 | -0.46 | -0.03 | 2.01 | 0.34 | -1.45 | 0.67 | -0.71 | 0.96 | -1.43 |
Mitotic Spindle | -3.23 | -2.47 | -14.92 | -13.50 | -12.95 | -4.48 | -5.15 | -10.76 | -8.18 | -7.01 | -5.52 | -5.67 | -18.16 | -15.53 | -14.39 |
None | 0.09 | 4.27 | -3.55 | -3.47 | -5.11 | 2.79 | -1.83 | 1.20 | -1.41 | 2.63 | 2.67 | -1.64 | -0.37 | -2.87 | 1.36 |
Nuclear Periphery | 3.99 | -11.62 | 19.17 | 12.20 | 26.78 | 5.61 | -0.64 | 14.54 | 7.49 | 12.51 | 6.85 | -6.41 | 23.29 | 13.63 | 24.79 |
Nuclear Periphery Foci | 2.03 | 16.22 | 15.14 | 10.91 | -2.32 | -4.25 | 13.73 | 4.00 | 7.67 | -9.40 | -2.20 | 20.52 | 11.88 | 12.68 | -8.94 |
Nucleolus | 1.65 | 3.58 | -4.92 | -5.72 | -6.56 | -0.20 | -6.03 | -4.40 | -4.44 | 2.11 | 1.50 | -3.68 | -6.38 | -7.08 | -3.81 |
Nucleus | 0.54 | 0.36 | -9.13 | -9.31 | -9.11 | -0.69 | -12.05 | -5.11 | -3.89 | 10.31 | -0.12 | -11.52 | -10.55 | -9.97 | 5.63 |
Peroxisomes | -1.23 | 3.42 | -4.32 | -0.19 | -6.07 | -1.65 | -2.47 | -1.28 | 1.47 | 0.99 | -2.04 | -0.14 | -4.62 | 0.90 | -4.32 |
Vacuole | -1.65 | -0.88 | -12.07 | -10.61 | -11.90 | -4.28 | -8.42 | -12.60 | -7.65 | -5.17 | -4.56 | -8.34 | -17.47 | -12.30 | -10.39 |
Vacuole Periphery | 2.23 | 4.16 | -2.14 | -4.26 | -5.75 | -3.55 | -0.14 | -5.73 | -1.20 | -5.55 | -2.31 | 2.35 | -5.74 | -2.84 | -7.59 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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