Standard name
Human Ortholog
Description Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0.06 0.06 0.09 0.07 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 1.0 0.96 0.87 – 0.74 0.71 0.7 0.65 0.66 0.98 0.99 0.99 0.96 0.93 0.95 0.98 0.97 0.96 0.93 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.08 0.06 0.09 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 – 0.16 0.26 0.24 0.32 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 8 1 7 8 6 0 1 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 1 – 1 1 4 1 1 0 1 1 1 2 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 3 2 0 6 8 – 9 12 15 18 19 10 16 13 1 6 11 0 0 0 0 0 0
Cytoplasm 251 132 54 133 195 166 – 262 133 180 132 181 439 557 672 86 111 132 255 129 65 111 322 450
Endoplasmic Reticulum 1 1 0 1 9 8 – 27 12 22 22 25 6 7 10 1 2 4 1 1 1 1 6 6
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 1 1 3 16 – 56 49 63 65 81 0 0 2 1 4 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 – 1 0 0 0 2 0 2 1 1 2 0 0 0 0 2 1 2
Nuclear Periphery 0 0 0 0 0 0 – 0 1 1 0 3 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 2 – 26 6 8 2 4 0 0 4 2 1 0 2 1 0 2 6 4
Unique Cell Count 252 133 54 133 204 191 355 187 258 204 275 446 563 681 90 119 139 261 134 68 120 345 473
Labelled Cell Count 255 136 57 135 214 201 391 215 300 248 322 455 584 703 94 128 150 261 134 68 120 345 473


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.3 19.7 10.4 14.8 16.6 14.3 – 12.4 11.5 11.8 12.6 11.8 19.9 19.9 19.0 23.2 25.8 25.3 16.7 19.6 20.1
Std Deviation (1e-4) 3.2 2.6 1.9 2.7 3.5 2.9 – 3.2 2.7 2.9 4.2 2.8 4.0 3.8 4.1 8.2 6.6 6.7 4.1 4.1 4.7
Intensity Change (Log2) – – – 0.51 0.67 0.45 – 0.25 0.15 0.18 0.27 0.18 0.93 0.94 0.87 1.15 1.31 1.28 0.68 0.91 0.95


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -1.6 -2.8 – -4.3 -4.5 -4.7 -5.1 -5.1 -0.9 -0.8 -0.9 -1.6 -2.0 -1.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 2.8 3.9 3.7 4.5 4.3 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.4848 24.9504 22.7013 20.9308 20.8481 22.6839 14.3525 23.1046 22.6843 20.6935 17.9718 22.4563 16.3875 28.5463 26.0074 25.9483 24.3396 24.7413
Actin 0.0342 0.0047 0.0009 0.0022 0.0052 0.0051 0.0245 0.0011 0.0019 0.0123 0.0039 0.0001 0.0194 0.0007 0.0133 0.0001 0.0004 0.0079
Bud 0.0015 0.0002 0.0007 0.0009 0.0003 0.0003 0.0013 0.0006 0.0002 0.0009 0.0096 0.0003 0.0015 0.0001 0.0004 0.0003 0.0044 0.0002
Bud Neck 0.0013 0.0005 0.0006 0.0014 0.0008 0.0039 0.0018 0.0002 0.0012 0.0004 0.0022 0.0007 0.0016 0.0001 0.0002 0.0003 0.0005 0.0008
Bud Periphery 0.0016 0.0001 0.0007 0.001 0.0004 0.0004 0.0013 0.0003 0.0001 0.001 0.0028 0.0001 0.0007 0 0.0004 0 0.0077 0.0004
Bud Site 0.0065 0.0014 0.0006 0.0024 0.0007 0.0003 0.0095 0.0067 0.0008 0.0005 0.0051 0.0003 0.0192 0.0002 0.0005 0.0001 0.0031 0.0004
Cell Periphery 0.0013 0.0003 0.0002 0.0004 0.0001 0.0001 0.0007 0.0002 0.0001 0.0002 0.0005 0 0.0003 0 0.0001 0 0.0003 0.0003
Cytoplasm 0.6565 0.9781 0.9378 0.8971 0.8795 0.928 0.6747 0.9703 0.9724 0.9041 0.7713 0.978 0.6353 0.9905 0.9299 0.9649 0.9073 0.8996
Cytoplasmic Foci 0.0308 0.0019 0.0089 0.0117 0.0082 0.0092 0.0287 0.005 0.003 0.0033 0.0425 0.0046 0.0741 0.0011 0.0035 0.0033 0.0041 0.0132
Eisosomes 0.0003 0 0 0 0 0 0.0003 0 0 0.0001 0 0 0.0001 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0459 0.0018 0.0054 0.0118 0.0034 0.0125 0.0285 0.0011 0.0027 0.0044 0.0039 0.002 0.0163 0.0025 0.0026 0.0024 0.0016 0.0148
Endosome 0.0759 0.0016 0.0128 0.0244 0.0438 0.0146 0.0534 0.0053 0.0061 0.0366 0.0775 0.0087 0.1126 0.002 0.0123 0.017 0.0089 0.0063
Golgi 0.0126 0.0021 0.0015 0.0047 0.0043 0.0086 0.022 0.0045 0.0043 0.0087 0.0335 0.0007 0.0177 0.0003 0.0047 0.0003 0.0003 0.008
Lipid Particles 0.0141 0.0003 0.0033 0.0043 0.0097 0.0017 0.0033 0.0012 0.0013 0.0012 0.0087 0.0001 0.0249 0.0001 0.0036 0 0.0001 0.0075
Mitochondria 0.0118 0.0003 0.0028 0.0074 0.0027 0.0052 0.009 0.0006 0.0014 0.0088 0.0085 0.0007 0.0085 0.0001 0.0102 0.0001 0.0016 0.0244
None 0.0048 0.0002 0.0005 0.002 0.0013 0.0003 0.0066 0.0008 0.0002 0.0008 0.0086 0.0002 0.0046 0.0003 0.0006 0.0002 0.0008 0.0005
Nuclear Periphery 0.0163 0.0015 0.0052 0.0029 0.0107 0.0012 0.0449 0.0003 0.0008 0.0024 0.0014 0.0003 0.0114 0.001 0.0069 0.0019 0.0046 0.0016
Nucleolus 0.0015 0 0.0006 0.0009 0.0006 0 0.0009 0 0.0001 0 0.0005 0 0.0006 0 0 0 0.0043 0
Nucleus 0.0142 0.0022 0.0029 0.0058 0.0028 0.0014 0.0124 0.0005 0.0009 0.0013 0.0026 0.001 0.0066 0.0006 0.0071 0.0008 0.0263 0.0007
Peroxisomes 0.0025 0.0003 0.0019 0.001 0.0021 0.0033 0.0011 0.0002 0.0003 0.0001 0.0022 0.0001 0.0146 0 0.0011 0 0.0002 0.0109
Punctate Nuclear 0.0147 0.0001 0.0005 0.0005 0.0048 0.0002 0.0306 0.0001 0.0002 0.0002 0.0023 0.0001 0.0155 0.0002 0.0004 0 0.0007 0.0005
Vacuole 0.0406 0.0021 0.0091 0.0144 0.0136 0.0028 0.0344 0.0008 0.0017 0.0085 0.01 0.0016 0.0116 0.0003 0.0014 0.0052 0.0181 0.0011
Vacuole Periphery 0.0108 0.0003 0.0029 0.0028 0.0048 0.0008 0.0099 0.0002 0.0003 0.0043 0.0025 0.0004 0.003 0.0001 0.0008 0.003 0.0049 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.4626 96.1084 135.1262 77.2715 88.0377 93.3133 82.471 86.2983 86.0397 77.9158
Translational Efficiency 2.0489 1.6456 1.0676 1.9417 1.706 1.7163 1.5742 1.8439 1.6341 1.6859

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1966 1399 87 1099 1510 896 1739 1003 3476 2295 1826 2102

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1412.75 1738.21 2207.03 2078.89 1318.28 1666.73 1859.55 2125.14 1371.71 1710.30 1876.11 2100.96
Standard Deviation 228.11 338.88 299.45 449.01 233.04 296.72 388.68 418.99 234.98 324.95 391.95 435.56
Intensity Change Log 2 — 0.299096 0.643600 0.557307 — 0.338364 0.496297 0.688901 — 0.318184 0.574371 0.622328

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000157 0.001511 0.001423 0.000251 0.000451 0.000154 0.001818 0.000140 0.000272 0.000219 0.001611
Bud Neck 0.000111 0.000308 0.010813 0.003040 0.000217 0.000316 0.000627 0.002017 0.000157 0.000311 0.001112 0.002552
Bud Site 0.005161 0.006878 0.033032 0.025424 0.023012 0.012178 0.007958 0.022777 0.012916 0.008947 0.009152 0.024161
Cell Periphery 0.000099 0.000301 0.005605 0.006944 0.000580 0.000378 0.001212 0.004046 0.000308 0.000331 0.001421 0.005561
Cytoplasm 0.938340* 0.931600* 0.588925* 0.743640* 0.742163* 0.891545* 0.926660* 0.804513* 0.853119* 0.915962* 0.910569* 0.772686*
Cytoplasmic Foci 0.034724 0.025631 0.054565 0.014404 0.138442 0.049327 0.007512 0.014761 0.079780 0.034882 0.009754 0.014575
Eisosomes 0.000001 0.000001 0.000012 0.000011 0.000003 0.000004 0.000001 0.000010 0.000002 0.000002 0.000002 0.000011
Endoplasmic Reticulum 0.000605 0.000730 0.007631 0.001079 0.004747 0.001656 0.000169 0.000586 0.002404 0.001091 0.000524 0.000844
Endosome 0.000697 0.001773 0.014501 0.007609 0.004873 0.003631 0.000769 0.004972 0.002511 0.002499 0.001423 0.006351
Golgi 0.008456 0.011696 0.032975 0.012408 0.031678 0.017385 0.004284 0.010589 0.018544 0.013917 0.005651 0.011540
Lipid Particles 0.000401 0.001442 0.025310 0.022570 0.000711 0.001748 0.005269 0.012706 0.000536 0.001562 0.006224 0.017863
Mitochondria 0.000255 0.000102 0.000055 0.001055 0.000725 0.001696 0.000039 0.001698 0.000459 0.000724 0.000039 0.001362
Mitotic Spindle 0.000363 0.002267 0.008321 0.005715 0.003029 0.003837 0.000510 0.001807 0.001521 0.002880 0.000883 0.003850
None 0.001319 0.001472 0.000405 0.000353 0.000251 0.000568 0.000847 0.000354 0.000855 0.001119 0.000826 0.000353
Nuclear Periphery 0.000006 0.000031 0.000359 0.000191 0.000063 0.000037 0.000025 0.000061 0.000031 0.000033 0.000041 0.000129
Nuclear Periphery Foci 0.000229 0.003358 0.015910 0.003652 0.001387 0.001739 0.001735 0.002861 0.000732 0.002726 0.002410 0.003275
Nucleolus 0.000028 0.000023 0.000080 0.000221 0.000094 0.000480 0.000058 0.000127 0.000057 0.000201 0.000059 0.000176
Nucleus 0.002540 0.004226 0.016758 0.008617 0.004461 0.002731 0.008653 0.007112 0.003374 0.003642 0.009039 0.007899
Peroxisomes 0.000096 0.000066 0.000263 0.000912 0.000131 0.000247 0.000080 0.000432 0.000111 0.000137 0.000089 0.000683
Vacuole 0.002932 0.004860 0.175463 0.134298 0.008345 0.005223 0.031328 0.100938 0.005284 0.005002 0.038195 0.118380
Vacuole Periphery 0.003582 0.003079 0.007509 0.006435 0.034835 0.004821 0.002110 0.005815 0.017159 0.003759 0.002367 0.006139

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.86 -6.10 -4.73 -4.36 0.42 -2.25 2.21 -1.99 -1.70 -2.11 -3.66 -2.83 -3.77 -3.40 -3.50
Bud Neck -4.27 -7.06 -11.80 -10.94 5.03 -2.53 -7.49 -11.70 -11.02 -8.45 -4.82 -9.55 -16.23 -15.10 -8.03
Bud Site -1.89 -5.68 -9.25 -8.27 1.85 6.83 11.16 0.11 -4.53 -6.60 4.51 3.08 -7.09 -9.47 -8.54
Cell Periphery -5.89 -4.00 -9.54 -9.20 -0.61 3.06 -5.36 -7.02 -7.48 -5.42 -0.72 -8.86 -11.86 -11.78 -8.61
Cytoplasm 1.57 11.48 22.99 21.35 -4.78 -16.08 -23.46 -4.67 9.98 15.14 -12.45 -9.37 13.56 23.04 20.53
Cytoplasmic Foci 3.85 -3.84 9.17 5.27 7.38 17.10 29.18 27.16 11.50 -5.12 16.33 28.45 26.22 11.77 -3.94
Eisosomes 2.74 -6.09 -8.27 -8.58 1.09 -1.64 5.46 -3.01 -2.00 -3.49 -1.00 -0.74 -6.64 -5.96 -6.50
Endoplasmic Reticulum -0.52 -1.35 -1.05 -0.82 1.28 4.48 7.95 7.15 2.79 -2.59 4.05 5.42 5.35 1.67 -1.01
Endosome -4.78 -6.77 -6.12 -4.90 3.63 2.09 15.53 0.24 -1.17 -5.33 0.01 5.32 -5.13 -4.85 -6.69
Golgi -2.77 -4.91 -1.37 0.53 4.20 7.11 20.72 11.71 3.70 -3.64 4.20 16.34 6.77 2.72 -4.03
Lipid Particles -5.90 -6.40 -11.90 -11.26 0.81 -4.13 -8.13 -10.58 -9.50 -5.74 -7.05 -9.90 -15.57 -14.52 -8.92
Mitochondria 2.76 5.52 -2.19 -2.72 -2.91 -1.04 4.00 -0.88 0.22 -1.77 -0.72 5.42 -1.66 -0.77 -2.72
Mitotic Spindle -5.13 -4.21 -3.94 -2.31 1.55 -0.84 3.80 1.62 2.43 -2.65 -2.98 1.76 -2.81 -0.92 -3.76
None -0.55 7.22 8.25 4.38 0.17 -4.96 -5.71 -2.92 2.67 4.27 -1.57 0.36 7.11 4.78 4.44
Nuclear Periphery -2.81 -7.60 -3.95 -3.35 2.85 1.10 1.47 -0.42 -2.53 -4.15 -0.14 -1.48 -3.87 -3.95 -3.50
Nuclear Periphery Foci -4.17 -7.65 -11.62 -0.46 5.85 -0.74 -0.93 -3.96 -4.79 -4.75 -4.01 -6.48 -10.66 -1.51 -3.62
Nucleolus 1.55 -5.97 -3.24 -3.32 -2.26 -0.96 0.68 -0.74 0.85 -4.37 -0.92 -0.03 -3.01 0.09 -3.85
Nucleus -6.51 -4.67 -10.66 -8.61 1.79 1.49 -4.28 -3.23 -6.99 0.90 -0.51 -9.05 -9.32 -10.91 -0.50
Peroxisomes 2.39 -3.58 -2.96 -3.07 -2.04 -1.37 4.32 -4.72 -1.56 -5.60 -0.78 2.03 -3.91 -3.60 -4.06
Vacuole -3.68 -7.00 -22.70 -22.35 1.41 3.71 -13.70 -18.97 -19.57 -13.64 0.49 -16.00 -29.48 -29.52 -18.71
Vacuole Periphery 0.57 -2.17 -2.15 -2.56 0.85 11.28 13.12 11.48 -0.39 -4.40 10.44 12.51 9.30 -2.26 -5.62
Standard name
Human Ortholog
Description Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Gim3

Gim3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gim3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available