Standard name
Human Ortholog
Description Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0 0 0 0 0 0 0 0.09 0.13 0.11 0.1 0.17 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.21 0.12 0.11 0.25 0.2 0.24 0.4 0.33 0.4 0.36 0 0 0.05 0.07 0 0 0 0 0 0 0 0
Nucleus 0.94 0.89 0.86 0.87 0.88 0.81 0.82 0.75 0.77 0.75 0.67 0.76 0.84 0.8 0.79 0.76 0.66 0.63 0.8 0.83 0.79 0.84 0.8 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.16 0.1 0.07 0.11 0.19 0.16 0.17 0.25 0.24 0.26 0.27 0.16 0.18 0.18 0.05 0.07 0.07 0.13 0.08 0.09 0.07 0.09 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0.07 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 3 3 2 2 0 1 0 0 0 0 0 0 4 4 2 2 2 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2 1 2 2 8 12 10 19 4 24 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 6 18 7 13 19 16 8 11 9 9 11 29 25 11 25 33 0 0 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 3 8 0 2 2 0 1 0
Endosome 1 2 1 0 1 0 0 4 0 0 0 0 0 1 1 3 4 2 4 2 1 1 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 1 3 0 0 0 0 0
Mitochondria 2 6 48 33 46 77 113 162 130 138 87 113 3 1 12 7 2 5 4 2 5 0 0 2
Nucleus 383 168 193 243 362 248 460 517 250 310 147 241 106 171 185 80 95 97 355 165 189 101 148 149
Nuclear Periphery 1 6 2 6 6 2 13 17 4 9 5 2 0 3 4 0 1 0 1 0 1 0 0 0
Nucleolus 41 30 22 19 44 57 88 116 83 100 57 86 20 39 41 5 10 11 57 15 20 8 16 41
Peroxisomes 0 1 0 0 0 0 1 0 0 0 0 0 0 0 4 1 0 0 0 0 0 0 0 0
SpindlePole 2 1 0 0 0 1 3 4 2 0 0 1 0 1 3 1 2 0 1 0 1 2 1 0
Vac/Vac Membrane 2 2 1 2 3 0 2 7 0 2 1 1 0 1 0 3 12 11 9 3 15 3 8 16
Unique Cell Count 409 188 224 278 411 306 562 685 326 415 218 318 126 215 234 105 143 153 445 198 241 121 186 221
Labelled Cell Count 437 223 275 322 471 400 710 861 489 591 310 478 140 248 276 115 156 168 445 198 241 121 186 221


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 7.5 5.7 6.3 6.1 5.4 5.2 5.4 4.7 4.8 4.7 4.6 8.2 7.8 6.9 9.4 10.5 11.4 7.8 7.2 8.6
Std Deviation (1e-4) 1.5 1.6 1.6 2.1 1.2 1.4 1.5 1.6 1.6 1.2 1.5 1.4 2.3 2.0 1.9 2.9 2.6 3.1 1.8 1.7 2.0
Intensity Change (Log2) – – – 0.14 0.1 -0.08 -0.13 -0.09 -0.28 -0.25 -0.28 -0.32 0.52 0.45 0.26 0.71 0.87 0.99 0.44 0.33 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 0 1.0 0.5 -0.3 -0.2 0 0.8 0.1 2.5 4.2 3.4 3.0 5.0 5.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.9 -3.5 1.0 -0.4 0.7 0 0 0 0 -4.8 -7.0 -5.2 -3.3 -5.5 -5.0
Nucleus 0.4 0.7 -1.6 -1.5 -3.4 -2.8 -3.4 -4.7 -3.0 -0.5 -1.8 -2.0 -2.2 -4.5 -5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.2 0.3 2.8 2.1 2.6 0 0 0 0 1.7 2.5 2.4 -1.6 -0.9 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 4.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0853 7.5412 6.6831 6.0528 6.2336 6.4381 4.8616 6.8658 6.1539 5.5919 5.6095 5.8388 5.6978 6.7175 6.0461 6.0352 6.6033 6.0665
Actin 0.0709 0 0.015 0.0001 0.0002 0.0012 0.0154 0 0.0018 0.0008 0.0002 0.001 0.0481 0 0.0034 0.0002 0.0011 0
Bud 0.0011 0 0.0002 0.0008 0.0001 0.0001 0.0011 0 0.0003 0.0002 0.0002 0.0001 0.0003 0 0.0003 0 0.0001 0
Bud Neck 0.0144 0.0001 0.0004 0.0021 0.0008 0.0011 0.0025 0 0.0004 0.0003 0.001 0.001 0.001 0 0.0003 0.0001 0.0021 0.0011
Bud Periphery 0.0018 0 0.0003 0.0027 0.0003 0.0003 0.0024 0 0.0005 0.0008 0.0003 0.0001 0.0004 0 0.0005 0.0001 0.0003 0.0001
Bud Site 0.0128 0 0.001 0.0026 0.0002 0.0004 0.0058 0 0.0022 0.0003 0.0009 0.0001 0.0019 0 0.0034 0.0001 0.0003 0.0001
Cell Periphery 0.0007 0 0.0001 0.001 0.0001 0.0002 0.0005 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.0048 0 0.0001 0.0002 0.0002 0.0001 0.0109 0 0.0007 0.0005 0.0051 0.0001 0.0039 0.0001 0.0025 0.0001 0.0004 0.0001
Cytoplasmic Foci 0.0171 0 0.0025 0 0.0043 0.0001 0.0188 0 0.0041 0.0049 0.0092 0.0055 0.0066 0 0.0021 0 0.0006 0.0027
Eisosomes 0.0007 0 0.0002 0 0 0.0001 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0055 0 0.0001 0.0001 0.0006 0.0001 0.0037 0 0.0009 0.0015 0.0009 0.0001 0.0024 0 0.0011 0 0.0004 0
Endosome 0.0257 0 0.005 0.0002 0.0081 0.0009 0.0149 0 0.004 0.0048 0.0231 0.0059 0.0051 0 0.0042 0 0.004 0.0006
Golgi 0.011 0 0.0026 0 0.0022 0.0007 0.006 0 0.0013 0.0012 0.0049 0.0058 0.0052 0 0.0012 0 0.0013 0.0002
Lipid Particles 0.0248 0 0.0054 0.0001 0.0639 0.0006 0.0286 0 0.0038 0.0153 0.0275 0.0056 0.012 0 0.0061 0 0.0095 0.0012
Mitochondria 0.0127 0.0001 0.0139 0.0018 0.0017 0.0139 0.027 0.0001 0.0014 0.0065 0.0178 0.0068 0.0024 0.0001 0.004 0.0007 0.0013 0.0002
None 0.0065 0 0.0001 0.0001 0.0001 0 0.0045 0 0.0002 0.0002 0.0008 0.0001 0.0014 0 0.0028 0.0001 0.0006 0
Nuclear Periphery 0.0227 0.0007 0.0009 0.0011 0.0034 0.0004 0.0161 0.0005 0.0084 0.0285 0.0079 0.0033 0.0206 0.0011 0.0166 0.038 0.0502 0.0006
Nucleolus 0.0591 0.0231 0.031 0.0502 0.1562 0.0642 0.0308 0.0136 0.018 0.0197 0.1064 0.0825 0.0456 0.0256 0.0401 0.0223 0.1667 0.0659
Nucleus 0.6695 0.9747 0.9109 0.9339 0.7499 0.9138 0.7597 0.9856 0.9431 0.8808 0.7696 0.8665 0.8018 0.9727 0.9009 0.9359 0.7339 0.9206
Peroxisomes 0.0075 0 0.0013 0.0001 0.0003 0.0001 0.0315 0 0.004 0.0212 0.0039 0.011 0.0249 0 0.0037 0 0.0008 0.0005
Punctate Nuclear 0.0143 0.0011 0.0044 0.0003 0.0062 0.0005 0.0104 0.0002 0.0031 0.0033 0.0124 0.0015 0.0131 0.0002 0.0053 0.0019 0.0238 0.0058
Vacuole 0.013 0 0.0028 0.0023 0.0006 0.0006 0.0049 0 0.001 0.0034 0.0051 0.0016 0.0012 0.0001 0.0008 0.0001 0.0011 0.0001
Vacuole Periphery 0.0033 0 0.0017 0.0004 0.0005 0.0004 0.0046 0 0.0004 0.0059 0.0026 0.0013 0.0016 0.0001 0.0007 0.0004 0.0012 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.8141 22.427 24.2756 32.2373 23.8734 24.8439 30.2172 29.4159 27.9979 33.198
Translational Efficiency 0.9225 0.6933 0.7601 0.5709 0.7645 0.925 0.6034 0.552 0.6484 0.6751

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
131 886 172 1276 1659 1510 422 579 1790 2396 594 1855

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 767.08 1040.56 1352.71 1043.85 759.65 938.15 1204.80 1171.85 760.19 976.02 1247.63 1083.80
Standard Deviation 128.91 163.82 167.82 164.42 150.28 132.38 148.70 165.93 148.84 153.01 168.42 175.24
Intensity Change Log 2 — 0.439911 0.818404 0.444465 — 0.304484 0.665387 0.625381 — 0.374115 0.744295 0.537318

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000762 0.002117 0.002845 0.001473 0.000598 0.001158 0.001060 0.001085 0.000610 0.001512 0.001577 0.001352
Bud Neck 0.006746 0.009677 0.015709 0.034324 0.003609 0.008779 0.016001 0.025134 0.003839 0.009111 0.015917 0.031456
Bud Site 0.000720 0.001937 0.002385 0.010019 0.000733 0.000938 0.001287 0.001825 0.000732 0.001308 0.001605 0.007461
Cell Periphery 0.000087 0.000069 0.000083 0.000238 0.000199 0.000056 0.000033 0.000061 0.000191 0.000061 0.000047 0.000183
Cytoplasm 0.002808 0.000304 0.000562 0.005778 0.007420 0.000687 0.000106 0.001327 0.007082 0.000545 0.000238 0.004389
Cytoplasmic Foci 0.001231 0.000968 0.000190 0.003558 0.000969 0.000747 0.000018 0.000103 0.000988 0.000829 0.000068 0.002479
Eisosomes 0.000039 0.000038 0.000065 0.000043 0.000026 0.000034 0.000054 0.000035 0.000027 0.000036 0.000057 0.000040
Endoplasmic Reticulum 0.003891 0.004006 0.007168 0.004354 0.004087 0.003398 0.002557 0.002166 0.004072 0.003623 0.003892 0.003671
Endosome 0.000422 0.000924 0.000218 0.007756 0.000482 0.000616 0.000060 0.000316 0.000477 0.000730 0.000106 0.005434
Golgi 0.000910 0.000826 0.000269 0.004123 0.000357 0.000533 0.000090 0.000154 0.000398 0.000641 0.000141 0.002884
Lipid Particles 0.003935 0.000295 0.000312 0.000642 0.000319 0.000232 0.000041 0.000124 0.000583 0.000255 0.000120 0.000480
Mitochondria 0.003499 0.003603 0.002401 0.005356 0.001933 0.002365 0.001472 0.005903 0.002047 0.002823 0.001741 0.005526
Mitotic Spindle 0.004100 0.011553 0.002333 0.053967 0.001133 0.004717 0.000192 0.011048 0.001350 0.007245 0.000812 0.040571
None 0.013071 0.000950 0.001027 0.005556 0.020367 0.001759 0.000770 0.002702 0.019833 0.001460 0.000845 0.004665
Nuclear Periphery 0.003180 0.000263 0.000410 0.002662 0.000279 0.000365 0.000141 0.000982 0.000491 0.000327 0.000219 0.002137
Nuclear Periphery Foci 0.002045 0.000402 0.000383 0.001243 0.001405 0.000583 0.000028 0.000270 0.001452 0.000516 0.000131 0.000939
Nucleolus 0.088171 0.078945 0.053253 0.018426 0.076977 0.081466 0.049229 0.024819 0.077796 0.080534 0.050394 0.020422
Nucleus 0.855518* 0.880763* 0.902559* 0.827536* 0.877565* 0.888611* 0.924790* 0.920013* 0.875951* 0.885709* 0.918353* 0.856401*
Peroxisomes 0.005858 0.001112 0.004674 0.001149 0.000774 0.000685 0.001236 0.000480 0.001146 0.000843 0.002231 0.000940
Vacuole 0.002602 0.000593 0.002854 0.008798 0.000516 0.001201 0.000677 0.001006 0.000668 0.000976 0.001308 0.006366
Vacuole Periphery 0.000404 0.000655 0.000298 0.003000 0.000254 0.001072 0.000159 0.000447 0.000265 0.000918 0.000199 0.002203

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.57 -6.95 -5.52 0.42 4.54 -0.92 -7.02 -5.38 -0.54 -1.20 -1.82 -9.94 -12.06 -0.33 1.35
Bud Neck -1.98 -4.54 -11.63 -11.87 -4.53 -7.02 -10.24 -12.43 -9.32 -4.16 -9.56 -11.78 -19.19 -14.96 -7.19
Bud Site -2.24 -5.70 -6.42 -4.82 -3.90 -1.23 -2.21 -5.21 -4.28 -0.96 -2.57 -3.59 -7.31 -6.24 -4.11
Cell Periphery 0.42 -0.40 -2.48 -4.76 -3.83 5.43 6.51 5.03 -1.29 -3.94 5.38 5.83 1.59 -5.51 -6.48
Cytoplasm 2.39 2.15 -2.28 -6.50 -6.21 5.38 5.93 4.56 -1.24 -2.50 5.66 5.97 2.32 -6.22 -6.98
Cytoplasmic Foci 0.37 1.63 -0.86 -1.37 -3.04 0.46 2.65 2.46 1.79 -2.11 0.50 2.70 -0.38 -0.93 -3.31
Eisosomes 0.17 -3.36 -0.99 -3.46 4.15 -2.52 -11.62 -6.81 -1.84 5.34 -3.61 -12.96 -10.90 -3.92 6.10
Endoplasmic Reticulum -0.19 -3.23 -1.62 -2.43 2.52 2.25 4.41 4.47 2.08 0.47 1.66 -0.22 -0.95 -2.34 -0.52
Endosome -1.03 0.82 -3.94 -3.09 -4.17 -0.57 3.47 1.51 1.89 -3.10 -1.01 3.17 -3.74 -3.28 -4.39
Golgi 0.26 1.10 -1.29 -1.81 -2.72 -0.64 2.68 1.63 1.28 -2.71 -1.03 2.38 -2.40 -1.78 -2.98
Lipid Particles 1.37 1.33 1.30 -1.04 -0.51 0.24 2.42 1.21 0.76 -2.13 1.27 2.02 0.92 -0.62 -2.31
Mitochondria 0.01 0.70 -0.83 -0.89 -3.59 -0.51 0.34 -1.65 -1.44 -1.89 -0.93 0.08 -3.12 -2.34 -4.30
Mitotic Spindle -2.07 0.57 -7.64 -5.42 -7.80 -2.31 0.68 -3.36 -2.30 -3.52 -3.79 0.10 -9.31 -7.00 -9.33
None 2.27 2.24 1.54 -7.66 -7.29 9.47 10.05 8.60 -2.39 -4.64 9.93 10.27 8.27 -6.66 -8.89
Nuclear Periphery 1.16 1.05 0.32 -6.02 -5.12 -1.09 2.21 -3.31 -2.76 -3.72 0.82 1.03 -4.69 -6.36 -6.60
Nuclear Periphery Foci 1.28 1.26 0.45 -2.66 -2.61 2.20 6.01 4.80 1.27 -2.95 3.16 5.67 1.27 -1.59 -3.50
Nucleolus 1.07 2.30 6.94 13.90 5.44 -1.08 5.25 12.40 13.18 4.26 -0.68 5.34 17.95 20.95 7.42
Nucleus -1.60 -1.67 0.31 3.65 3.09 -1.97 -6.45 -3.76 -2.34 1.39 -1.99 -5.53 1.31 3.09 6.12
Peroxisomes 1.32 0.24 1.37 1.07 1.84 0.56 -1.99 1.02 0.61 4.31 1.10 -1.54 1.20 0.30 2.31
Vacuole 1.15 -0.69 -2.98 -6.49 -3.67 -1.51 -1.92 -2.93 -1.32 -1.52 -0.93 -3.87 -6.71 -6.21 -5.19
Vacuole Periphery -0.92 0.58 -3.21 -2.22 -3.43 -2.16 2.08 -1.28 1.81 -2.29 -2.53 1.06 -3.75 -0.93 -3.92
Standard name
Human Ortholog
Description Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tho2

Tho2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tho2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available