Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; N-terminally propionylated in vivo; mutation is functionally complemented by human FKBP1A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 – 0.06 0.05 0.07 0.07 0.08 0 0 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.96 1.0 0.99 1.0 – 0.92 0.91 0.9 0.84 0.83 1.0 0.99 0.99 0.97 0.99 0.99 1.0 0.97 0.89 0.91 0.9 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.05 0 0.06 0.09 0.11 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 3 0 8 3 1 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 1 4 0 0 0 – 0 2 0 2 1 0 0 0 0 0 0 0 1 1 0 2 2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 17 4 0 4 8 – 44 20 31 18 28 5 5 11 2 1 1 0 0 1 0 0 0
Cytoplasm 258 351 220 242 330 436 – 643 390 406 217 309 164 216 232 115 106 114 253 337 202 80 87 148
Endoplasmic Reticulum 0 3 1 0 3 0 – 35 19 26 22 41 0 1 2 2 3 6 0 0 3 1 1 1
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Golgi 0 0 0 0 0 0 – 0 1 0 2 1 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 4 0 0 0 – 3 6 2 7 13 0 1 0 0 1 0 0 1 2 0 0 0
Nucleus 0 1 4 0 0 0 – 1 1 1 1 0 0 0 0 0 0 0 0 2 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 1 0 1 – 1 4 7 3 3 0 0 0 0 0 1 0 1 1 1 2 1
Unique Cell Count 258 355 230 243 335 438 700 430 449 258 371 164 218 234 118 107 115 255 348 228 88 98 160
Labelled Cell Count 263 374 241 243 337 445 731 443 481 275 397 169 223 245 120 111 122 255 348 228 88 98 160


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.1 58.2 29.8 46.0 45.0 38.4 – 36.1 33.1 32.6 31.3 30.2 60.4 58.2 54.5 82.8 76.9 69.6 52.6 56.5 58.1
Std Deviation (1e-4) 8.5 11.0 10.2 11.1 10.2 8.5 – 7.9 7.9 8.1 8.3 7.4 12.0 15.7 15.2 23.8 22.3 27.6 14.6 20.4 18.4
Intensity Change (Log2) – – – 0.63 0.6 0.37 – 0.28 0.16 0.13 0.07 0.02 1.02 0.97 0.87 1.48 1.37 1.23 0.82 0.93 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 2.7 1.9 2.9 2.8 3.1 0 0 1.8 0 0 0
Cytoplasm 2.8 2.1 3.6 – -1.9 -2.3 -2.4 -4.2 -4.5 2.7 2.2 2.4 0.8 1.6 1.7
Endoplasmic Reticulum 0 0 0 – 3.1 2.8 3.4 4.2 5.0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0.7 1.3 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 34.408 40.8978 38.2951 36.8328 32.9712 37.9766 38.6175 50.1008 44.7169 42.0986 41.7176 42.9612 49.0926 59.5606 53.2308 54.052 48.0384 54.5547
Actin 0.0065 0.0002 0.0226 0.0001 0.0017 0.0008 0.0122 0.0001 0.002 0.0224 0.0034 0.0049 0.0381 0.0001 0.0022 0.0001 0.0013 0.0001
Bud 0.0009 0.0001 0.0006 0 0.0002 0.0001 0.0009 0 0.0006 0.0019 0.0009 0.0004 0.0003 0 0.0038 0 0.0009 0
Bud Neck 0.0007 0.0002 0.0004 0.0002 0.0003 0.0008 0.0014 0.0001 0.0003 0.0004 0.0005 0.0005 0.0002 0.0001 0.0005 0.0002 0.0004 0.0005
Bud Periphery 0.0007 0 0.0005 0 0.0002 0.0001 0.0008 0 0.0006 0.0011 0.0008 0.0009 0.0004 0 0.0047 0 0.0004 0
Bud Site 0.0018 0.0001 0.0011 0 0.0003 0.0002 0.0063 0 0.0007 0.0036 0.0011 0.0004 0.0008 0 0.0052 0 0.0011 0
Cell Periphery 0.0003 0 0.0001 0 0.0001 0.0002 0.0005 0 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0006 0 0.0003 0
Cytoplasm 0.8751 0.9966 0.9367 0.9962 0.8932 0.9532 0.8703 0.9932 0.9476 0.9018 0.947 0.905 0.8795 0.9967 0.9339 0.9966 0.9279 0.996
Cytoplasmic Foci 0.0101 0.0003 0.0063 0.0003 0.002 0.0048 0.0158 0.0001 0.0126 0.0244 0.006 0.0099 0.0079 0.0002 0.0071 0.0003 0.023 0.0004
Eisosomes 0.0003 0 0.0002 0 0.0001 0 0.0004 0 0 0.0001 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0028 0.0006 0.0014 0.0013 0.0008 0.0026 0.0054 0.0002 0.0019 0.0013 0.0012 0.0039 0.0034 0.0014 0.003 0.0004 0.0011 0.0008
Endosome 0.0179 0.0001 0.0051 0.0002 0.0083 0.0039 0.025 0 0.0076 0.0056 0.0169 0.0142 0.019 0.0001 0.006 0.0001 0.0072 0.0002
Golgi 0.0039 0 0.0014 0 0.0008 0.0003 0.0087 0 0.0024 0.0042 0.0065 0.0066 0.0071 0 0.0012 0 0.0014 0
Lipid Particles 0.0127 0 0.0038 0 0.0044 0.0017 0.0058 0 0.0022 0.0028 0.0009 0.0073 0.015 0 0.0014 0 0.007 0
Mitochondria 0.0156 0 0.0075 0 0.0509 0.0007 0.003 0 0.0026 0.0144 0.0042 0.015 0.005 0.0001 0.0057 0 0.0113 0
None 0.0062 0.0001 0.0028 0.0001 0.0002 0.005 0.0043 0.0001 0.0009 0.002 0.0003 0.0013 0.0022 0.0001 0.0016 0.0001 0.0014 0.0001
Nuclear Periphery 0.0116 0.0004 0.0023 0.0004 0.0014 0.0051 0.0111 0.0005 0.0014 0.0009 0.001 0.007 0.0054 0.0003 0.0014 0.001 0.0006 0.0004
Nucleolus 0.0017 0 0.0003 0 0.0002 0.0018 0.0014 0.0002 0.0003 0.0002 0.0001 0.0028 0.0005 0 0.0005 0 0.0009 0
Nucleus 0.0161 0.0009 0.0022 0.0008 0.0022 0.0077 0.01 0.005 0.002 0.0029 0.0015 0.0065 0.0024 0.0006 0.0027 0.0008 0.0019 0.001
Peroxisomes 0.0051 0 0.0012 0 0.0022 0.0001 0.0019 0 0.0032 0.0074 0.001 0.0033 0.0034 0 0.0007 0 0.0024 0
Punctate Nuclear 0.004 0 0.0013 0 0.0001 0.0071 0.0068 0 0.0004 0.0012 0.0005 0.0019 0.0015 0 0.0003 0.0001 0.0004 0
Vacuole 0.004 0.0002 0.0014 0.0003 0.0263 0.0029 0.0063 0.0002 0.0084 0.001 0.0052 0.004 0.0052 0.0001 0.014 0.0002 0.006 0.0002
Vacuole Periphery 0.0021 0 0.0009 0 0.0041 0.0008 0.0017 0.0001 0.0017 0.0004 0.0009 0.0039 0.0022 0 0.0033 0 0.003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 852.34 809.1144 734.0552 741.4723 767.1398 703.2307 826.0444 756.0478 798.4677 763.9726
Translational Efficiency 1.939 2.0738 2.2214 2.0003 2.3143 3.1339 2.1514 2.5254 2.3475 2.2801

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
262 143 2016 1636 325 2331 2340 1025 587 2474 4356 2661

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2361.03 3180.78 4849.14 5168.53 3073.37 3043.74 4384.76 4976.01 2755.43 3051.66 4599.68 5094.37
Standard Deviation 487.98 678.44 805.81 1070.67 576.94 561.95 977.90 1068.21 644.96 570.23 931.58 1073.82
Intensity Change Log 2 — 0.429964 1.038313 1.130338 — -0.013976 0.512676 0.695168 — 0.195842 0.764819 0.900511

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000165 0.000132 0.000058 0.000419 0.000159 0.000045 0.000100 0.000259 0.000162 0.000050 0.000081 0.000358
Bud Neck 0.000695 0.000467 0.000454 0.001671 0.000779 0.000190 0.000277 0.001088 0.000741 0.000206 0.000359 0.001447
Bud Site 0.008857 0.003403 0.001310 0.009029 0.004221 0.001614 0.001879 0.004977 0.006291 0.001717 0.001616 0.007468
Cell Periphery 0.007941 0.004235 0.001888 0.006146 0.011256 0.000268 0.000837 0.008733 0.009776 0.000498 0.001324 0.007142
Cytoplasm 0.830609* 0.898865* 0.909057* 0.851655* 0.804828* 0.957882* 0.927976* 0.871163* 0.816335* 0.954471* 0.919220* 0.859169*
Cytoplasmic Foci 0.022867 0.010006 0.003315 0.004363 0.017422 0.004523 0.001666 0.006940 0.019852 0.004840 0.002429 0.005356
Eisosomes 0.000002 0.000001 0.000001 0.000005 0.000002 0.000000 0.000001 0.000005 0.000002 0.000000 0.000001 0.000005
Endoplasmic Reticulum 0.001406 0.000299 0.000022 0.000105 0.001135 0.000074 0.000039 0.000084 0.001256 0.000087 0.000031 0.000097
Endosome 0.002238 0.000435 0.000049 0.000460 0.001378 0.000357 0.000089 0.000812 0.001762 0.000361 0.000070 0.000595
Golgi 0.061958 0.025448 0.001607 0.001904 0.060734 0.004054 0.000968 0.002387 0.061280 0.005291 0.001264 0.002090
Lipid Particles 0.010619 0.007266 0.002338 0.007462 0.017617 0.001046 0.001035 0.009084 0.014493 0.001406 0.001638 0.008087
Mitochondria 0.000055 0.000029 0.000057 0.002413 0.000202 0.000224 0.000095 0.002042 0.000137 0.000213 0.000078 0.002270
Mitotic Spindle 0.001270 0.001319 0.000166 0.001056 0.002071 0.000682 0.000126 0.000457 0.001713 0.000719 0.000145 0.000825
None 0.003093 0.002724 0.005579 0.002441 0.001270 0.002573 0.003837 0.003662 0.002083 0.002582 0.004643 0.002911
Nuclear Periphery 0.000088 0.000115 0.000108 0.000166 0.001801 0.000030 0.000039 0.000144 0.001037 0.000035 0.000071 0.000157
Nuclear Periphery Foci 0.006821 0.008781 0.001630 0.002649 0.013414 0.002238 0.000755 0.002651 0.010472 0.002616 0.001160 0.002650
Nucleolus 0.000085 0.000027 0.000050 0.000145 0.000230 0.000031 0.000197 0.000092 0.000165 0.000031 0.000129 0.000125
Nucleus 0.008567 0.008762 0.056131 0.041700 0.011812 0.013747 0.042137 0.034442 0.010364 0.013459 0.048613 0.038904
Peroxisomes 0.000040 0.000013 0.000010 0.000079 0.000029 0.000013 0.000056 0.000454 0.000034 0.000013 0.000035 0.000223
Vacuole 0.019639 0.020149 0.015392 0.064354 0.035398 0.008866 0.017447 0.049754 0.028364 0.009518 0.016496 0.058730
Vacuole Periphery 0.012983 0.007522 0.000777 0.001779 0.014245 0.001542 0.000444 0.000771 0.013682 0.001888 0.000598 0.001391

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.47 3.71 -2.39 -2.38 -3.37 3.57 0.68 -2.59 -5.53 -2.81 5.24 1.88 -3.12 -5.12 -3.97
Bud Neck 1.37 -0.85 -9.72 -11.33 -10.38 4.62 3.00 -3.89 -10.08 -8.38 5.79 1.71 -10.12 -17.41 -13.73
Bud Site 3.35 5.12 0.16 -3.72 -6.04 2.89 2.45 -1.68 -7.29 -7.34 5.24 5.57 -1.10 -6.98 -7.50
Cell Periphery 2.35 4.17 1.76 -1.26 -6.23 7.50 7.04 1.75 -9.43 -8.61 9.08 8.09 2.96 -13.49 -11.03
Cytoplasm -3.52 -4.08 2.34 7.10 15.17 -10.80 -7.96 -2.65 19.39 12.22 -13.61 -8.72 0.07 31.93 20.56
Cytoplasmic Foci 4.56 7.35 6.80 2.36 -2.13 7.81 9.35 4.90 -4.05 -6.79 10.51 11.60 8.70 -4.25 -7.41
Eisosomes 2.51 2.26 -3.33 -5.42 -5.29 4.79 2.49 -3.46 -5.26 -4.51 5.96 3.35 -4.91 -8.17 -6.95
Endoplasmic Reticulum 1.72 2.16 2.06 2.64 -2.94 2.26 2.34 2.27 0.13 -1.12 3.03 3.17 3.04 0.42 -2.73
Endosome 2.77 3.38 2.83 0.27 -3.59 4.96 6.82 2.18 -0.94 -1.98 4.40 5.50 3.85 -0.83 -3.39
Golgi 4.55 9.07 9.06 5.32 -0.16 10.26 10.85 10.63 4.13 -2.33 13.10 14.11 13.97 7.96 -2.26
Lipid Particles 1.39 4.06 0.33 -1.56 -8.74 6.09 5.95 2.34 -10.18 -9.73 7.64 7.12 2.32 -15.10 -13.55
Mitochondria 1.69 -0.71 -1.83 -1.87 -1.82 -0.02 1.07 -1.30 -1.28 -1.43 -0.36 0.99 -2.19 -2.02 -2.30
Mitotic Spindle -0.11 6.21 0.46 0.40 -1.78 1.64 2.38 2.03 1.50 -1.42 2.01 3.37 1.71 -0.12 -2.11
None 0.17 -1.07 0.45 0.90 10.59 -4.42 -6.44 -4.07 -1.35 0.65 -0.48 -2.41 -0.50 -0.03 6.24
Nuclear Periphery -1.13 -4.37 -8.11 -4.38 -5.04 1.14 1.12 1.05 -10.37 -8.62 1.16 1.08 0.96 -17.44 -10.38
Nuclear Periphery Foci -0.89 5.86 4.02 2.46 -6.43 7.72 8.84 7.11 -2.70 -10.36 8.42 10.34 8.01 -2.18 -12.24
Nucleolus 1.24 0.62 -1.33 -7.27 -5.61 1.03 0.11 0.72 -3.94 1.22 1.23 0.24 0.34 -8.11 0.17
Nucleus -0.07 -13.63 -11.32 -21.05 3.66 -0.04 -7.97 -6.09 -14.41 3.30 -0.84 -14.93 -12.68 -24.93 3.28
Peroxisomes 3.60 4.16 -1.68 -3.94 -4.22 1.65 -1.26 -1.13 -1.19 -1.04 3.31 -0.06 -1.28 -1.47 -1.27
Vacuole -0.14 -0.65 -16.96 -11.58 -19.62 5.63 2.76 -5.80 -16.33 -12.35 6.15 2.11 -15.18 -27.48 -23.10
Vacuole Periphery 1.40 5.01 4.76 2.01 -2.31 5.53 6.05 5.92 3.39 -2.49 6.97 7.86 7.53 2.59 -3.43
Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; N-terminally propionylated in vivo; mutation is functionally complemented by human FKBP1A
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Fpr1

Fpr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fpr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available