Standard name | |
---|---|
Human Ortholog | |
Description | Polyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 150 | 51 | 41 | 38 | 34 | 34 | 18 | 16 | 7 | – | 4 | 2 | 60 | 46 | 20 | 114 | 66 | 133 | 269 | 151 | 83 | 95 | 132 | 240 |
Bud | 52 | 23 | 32 | 38 | 73 | 72 | 86 | 71 | 70 | – | 35 | 61 | 30 | 50 | 64 | 28 | 18 | 33 | 12 | 26 | 6 | 13 | 21 | 23 |
Bud Neck | 0 | 0 | 3 | 2 | 5 | 1 | 5 | 3 | 0 | – | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 21 | 5 | 11 | 10 | 12 |
Bud Site | 7 | 2 | 6 | 6 | 17 | 26 | 29 | 19 | 15 | – | 5 | 9 | 7 | 3 | 4 | 8 | 2 | 6 | – | – | – | – | – | – |
Cell Periphery | 12 | 10 | 4 | 1 | 5 | 1 | 4 | 2 | 1 | – | 7 | 7 | 10 | 10 | 15 | 11 | 6 | 8 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 34 | 65 | 33 | 64 | 68 | 64 | 90 | 70 | 46 | – | 35 | 37 | 86 | 144 | 161 | 69 | 36 | 53 | 4 | 20 | 6 | 10 | 7 | 2 |
Endoplasmic Reticulum | 23 | 32 | 13 | 8 | 10 | 14 | 12 | 18 | 8 | – | 2 | 10 | 7 | 5 | 3 | 15 | 7 | 17 | 4 | 2 | 2 | 1 | 2 | 1 |
Endosome | 22 | 4 | 0 | 0 | 15 | 6 | 7 | 2 | 1 | – | 1 | 0 | 16 | 52 | 40 | 38 | 21 | 32 | 29 | 21 | 11 | 19 | 31 | 23 |
Golgi | 16 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 19 | 27 | 24 | 22 | 2 | 3 | 2 | 8 | 14 |
Mitochondria | 64 | 96 | 94 | 124 | 142 | 250 | 377 | 409 | 321 | – | 221 | 371 | 21 | 10 | 73 | 37 | 22 | 29 | 31 | 34 | 41 | 27 | 32 | 17 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 3 | 0 | – | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 59 | 17 | 9 | 9 | 64 | 43 | 69 | 36 | 22 | – | 12 | 28 | 47 | 117 | 53 | 33 | 24 | 38 | 40 | 6 | 24 | 16 | 14 | 21 |
SpindlePole | 26 | 2 | 8 | 2 | 32 | 27 | 55 | 42 | 25 | – | 19 | 29 | 14 | 22 | 15 | 50 | 38 | 61 | 3 | 2 | 2 | 10 | 4 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | – | 1 | 1 | 0 | 1 | 1 | 5 | 1 | 1 | 3 | 3 | 2 | 1 | 4 | 1 |
Unique Cell Count | 301 | 225 | 157 | 205 | 313 | 373 | 534 | 540 | 376 | 258 | 426 | 216 | 329 | 337 | 298 | 185 | 291 | 434 | 296 | 194 | 214 | 278 | 364 | |
Labelled Cell Count | 467 | 302 | 244 | 292 | 468 | 540 | 758 | 698 | 516 | 344 | 564 | 298 | 460 | 449 | 429 | 271 | 438 | 434 | 296 | 194 | 214 | 278 | 364 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.0 | 4.0 | 3.7 | 4.0 | 3.7 | 3.5 | 3.5 | 3.3 | – | 3.3 | 3.1 | 5.4 | 5.6 | 5.0 | 7.2 | 8.1 | 8.2 | 5.8 | 6.1 | 7.8 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.7 | 0.8 | 0.8 | 1.0 | 1.2 | 1.1 | 1.1 | – | 1.3 | 0.9 | 0.8 | 1.5 | 1.3 | 1.5 | 1.9 | 1.8 | 1.4 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.01 | -0.11 | -0.19 | -0.19 | -0.29 | – | -0.26 | -0.38 | 0.45 | 0.49 | 0.33 | 0.86 | 1.03 | 1.04 | 0.54 | 0.62 | 0.96 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.4 | -3.3 | -6.3 | 2.6 | 1.9 | 4.1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.7 | -1.4 | -0.4 | -3.3 | -2.8 | -2.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.3 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.0 | 0 | 1.0 | 0.6 | 0 | 0 |
Cytoplasm | 2.2 | 0.2 | -1.0 | -1.2 | -2.5 | -2.6 | – | -2.0 | -4.1 | 3.8 | 4.9 | 5.7 | 0.5 | -0.4 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -2.1 | -3.7 | -4.3 | -1.4 | -1.8 | -1.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.5 | 5.3 | 4.5 | 4.7 | 4.4 | 4.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.2 | 5.0 | 3.7 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -10.4 | -14.3 | -8.4 | -10.6 | -9.3 | -11.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.3 | 7.0 | 3.1 | 1.9 | 2.3 | 2.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.6 | 0.7 | -0.3 | 3.6 | 4.2 | 4.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9766 | 3.6906 | 3.7767 | 3.1202 | 2.9343 | 3.4399 | 2.751 | 2.819 | 2.5403 | 2.6938 | 2.4249 | 2.6367 | 0.2941 | 0.4573 | 0.6563 | 0.7119 | 0.7088 | 0.8346 |
Actin | 0.3967 | 0.3525 | 0.4703 | 0.4102 | 0.3533 | 0.4479 | 0.4454 | 0.334 | 0.5197 | 0.4753 | 0.3788 | 0.4719 | 0.3772 | 0.3655 | 0.3859 | 0.395 | 0.3987 | 0.4225 |
Bud | 0.0009 | 0.004 | 0.0157 | 0.0044 | 0.0015 | 0.0014 | 0.0052 | 0.0068 | 0.0346 | 0.0253 | 0.017 | 0.0014 | 0.003 | 0.003 | 0.0278 | 0.0126 | 0.0088 | 0.0011 |
Bud Neck | 0.0309 | 0.0175 | 0.0315 | 0.0291 | 0.0062 | 0.1371 | 0.0045 | 0.0122 | 0.0094 | 0.0037 | 0.028 | 0.1211 | 0.0088 | 0.0035 | 0.018 | 0.0009 | 0.0837 | 0.1051 |
Bud Periphery | 0.0023 | 0.0055 | 0.0554 | 0.0163 | 0.0027 | 0.0027 | 0.0078 | 0.0058 | 0.0677 | 0.0591 | 0.0319 | 0.0017 | 0.0076 | 0.0057 | 0.103 | 0.0751 | 0.064 | 0.0025 |
Bud Site | 0.0395 | 0.2038 | 0.091 | 0.0229 | 0.0133 | 0.004 | 0.028 | 0.1774 | 0.0294 | 0.0174 | 0.0061 | 0.0022 | 0.0423 | 0.0664 | 0.0611 | 0.0028 | 0.003 | 0.0027 |
Cell Periphery | 0.0089 | 0.0065 | 0.0085 | 0.011 | 0.0085 | 0.002 | 0.0005 | 0.0014 | 0.0009 | 0.0009 | 0.0005 | 0.0005 | 0.002 | 0.0011 | 0.0026 | 0.0011 | 0.0027 | 0.0013 |
Cytoplasm | 0.0511 | 0.1037 | 0.0905 | 0.1249 | 0.0998 | 0.0899 | 0.0303 | 0.1754 | 0.0853 | 0.0818 | 0.0639 | 0.1144 | 0.0467 | 0.0691 | 0.0535 | 0.0887 | 0.0932 | 0.0691 |
Cytoplasmic Foci | 0.1287 | 0.0986 | 0.0516 | 0.1324 | 0.1629 | 0.1203 | 0.1508 | 0.1028 | 0.0753 | 0.0846 | 0.1191 | 0.0928 | 0.1613 | 0.1251 | 0.0699 | 0.112 | 0.0762 | 0.1347 |
Eisosomes | 0.0015 | 0.0017 | 0.0016 | 0.0016 | 0.0008 | 0.0007 | 0.0007 | 0.0009 | 0.0005 | 0.0005 | 0.0003 | 0.0008 | 0.0026 | 0.0009 | 0.0024 | 0.0008 | 0.0022 | 0.0014 |
Endoplasmic Reticulum | 0.0037 | 0.0041 | 0.004 | 0.0038 | 0.0136 | 0.0012 | 0.0059 | 0.0033 | 0.0013 | 0.0025 | 0.0023 | 0.004 | 0.0031 | 0.0023 | 0.001 | 0.01 | 0.0044 | 0.0008 |
Endosome | 0.0393 | 0.029 | 0.022 | 0.0727 | 0.0487 | 0.017 | 0.063 | 0.0295 | 0.0196 | 0.0746 | 0.0681 | 0.0311 | 0.0363 | 0.0186 | 0.019 | 0.0236 | 0.0165 | 0.0079 |
Golgi | 0.0466 | 0.0371 | 0.0409 | 0.0699 | 0.0263 | 0.0418 | 0.0529 | 0.0296 | 0.0232 | 0.062 | 0.0439 | 0.0477 | 0.0467 | 0.0326 | 0.0239 | 0.0196 | 0.0205 | 0.0239 |
Lipid Particles | 0.0218 | 0.005 | 0.0043 | 0.0138 | 0.0281 | 0.009 | 0.0376 | 0.0026 | 0.0084 | 0.0119 | 0.0069 | 0.0062 | 0.0185 | 0.0032 | 0.0024 | 0.0056 | 0.0067 | 0.0056 |
Mitochondria | 0.0081 | 0.007 | 0.0086 | 0.0175 | 0.0172 | 0.0189 | 0.0112 | 0.0103 | 0.0251 | 0.0477 | 0.0654 | 0.0098 | 0.0184 | 0.0044 | 0.0303 | 0.0122 | 0.0162 | 0.0065 |
None | 0.0713 | 0.0572 | 0.0561 | 0.0243 | 0.0513 | 0.0261 | 0.0182 | 0.0656 | 0.0223 | 0.0099 | 0.006 | 0.0084 | 0.0896 | 0.1633 | 0.1332 | 0.1656 | 0.1505 | 0.1168 |
Nuclear Periphery | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.002 | 0.0001 | 0.0058 | 0.0006 | 0.0006 | 0.0015 | 0.0125 | 0.0074 | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0011 | 0.0001 |
Nucleolus | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0002 |
Nucleus | 0.0004 | 0.0004 | 0.0006 | 0.0009 | 0.0005 | 0.0004 | 0.0049 | 0.0013 | 0.0005 | 0.0007 | 0.017 | 0.0013 | 0.0006 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0003 |
Peroxisomes | 0.1415 | 0.0631 | 0.043 | 0.0331 | 0.1583 | 0.0772 | 0.1017 | 0.0361 | 0.0709 | 0.0337 | 0.096 | 0.0736 | 0.1302 | 0.1283 | 0.0615 | 0.0699 | 0.0455 | 0.0946 |
Punctate Nuclear | 0.0014 | 0.0007 | 0.0007 | 0.0007 | 0.002 | 0.0009 | 0.0218 | 0.002 | 0.0015 | 0.0017 | 0.0212 | 0.0013 | 0.0016 | 0.0035 | 0.0013 | 0.0006 | 0.0007 | 0.0025 |
Vacuole | 0.004 | 0.0015 | 0.0024 | 0.0077 | 0.0023 | 0.0008 | 0.0018 | 0.0017 | 0.0024 | 0.0032 | 0.0104 | 0.001 | 0.0022 | 0.0018 | 0.0013 | 0.0023 | 0.0037 | 0.0003 |
Vacuole Periphery | 0.0006 | 0.0004 | 0.0004 | 0.002 | 0.0006 | 0.0003 | 0.0011 | 0.0004 | 0.0012 | 0.0018 | 0.0042 | 0.0013 | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0008 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.3874 | 12.774 | 7.8304 | 10.1866 | 5.6387 | 8.6932 | 10.3888 | 11.0861 | 11.3759 | 10.6689 |
Translational Efficiency | 0.3423 | 0.3465 | 0.5946 | 0.4466 | 0.6506 | 0.4608 | 0.5339 | 0.3472 | 0.3956 | 0.4226 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2271 | 143 | 244 | 1180 | 1416 | 1610 | 1378 | 443 | 3687 | 1753 | 1622 | 1623 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 688.71 | 849.53 | 1203.44 | 906.73 | 684.86 | 765.73 | 957.14 | 948.75 | 687.23 | 772.57 | 994.19 | 918.20 |
Standard Deviation | 73.35 | 105.33 | 136.75 | 114.15 | 85.25 | 100.73 | 108.15 | 104.18 | 78.16 | 103.68 | 143.19 | 113.08 |
Intensity Change Log 2 | — | 0.302768 | 0.805196 | 0.396776 | — | 0.161027 | 0.482921 | 0.470219 | — | 0.233836 | 0.653488 | 0.433862 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.133924* | 0.125130* | 0.210631* | 0.330968* | 0.138058* | 0.152963* | 0.339611* | 0.410028* | 0.135512* | 0.150693* | 0.320208* | 0.352547* |
Bud Neck | 0.027740 | 0.013974 | 0.004187 | 0.011703 | 0.015037 | 0.013961 | 0.013799 | 0.009662 | 0.022861 | 0.013962 | 0.012353 | 0.011146 |
Bud Site | 0.040750 | 0.074230 | 0.127720* | 0.171928* | 0.025866 | 0.044066 | 0.173796* | 0.231714* | 0.035034 | 0.046526 | 0.166864* | 0.188247* |
Cell Periphery | 0.001209 | 0.002456 | 0.000377 | 0.001275 | 0.000633 | 0.001121 | 0.001092 | 0.001299 | 0.000988 | 0.001230 | 0.000984 | 0.001281 |
Cytoplasm | 0.090987 | 0.058380 | 0.075514 | 0.058762 | 0.065655 | 0.059020 | 0.067278 | 0.053986 | 0.081258 | 0.058968 | 0.068517 | 0.057458 |
Cytoplasmic Foci | 0.172579 | 0.220206* | 0.277086* | 0.077125 | 0.199937* | 0.170491 | 0.064813 | 0.044039 | 0.183086* | 0.174546 | 0.096746 | 0.068094 |
Eisosomes | 0.007121 | 0.002645 | 0.000335 | 0.001398 | 0.004889 | 0.003217 | 0.001038 | 0.001223 | 0.006263 | 0.003170 | 0.000932 | 0.001350 |
Endoplasmic Reticulum | 0.000348 | 0.002528 | 0.002107 | 0.003743 | 0.000608 | 0.001072 | 0.004404 | 0.003327 | 0.000448 | 0.001191 | 0.004058 | 0.003630 |
Endosome | 0.018513 | 0.063875 | 0.076845 | 0.033557 | 0.046141 | 0.047198 | 0.010175 | 0.006517 | 0.029123 | 0.048559 | 0.020204 | 0.026177 |
Golgi | 0.092845 | 0.123278* | 0.072939 | 0.023687 | 0.132149* | 0.158906* | 0.006446 | 0.005010 | 0.107940 | 0.156000* | 0.016449 | 0.018589 |
Lipid Particles | 0.019161 | 0.012374 | 0.020436 | 0.009579 | 0.037956 | 0.018540 | 0.009802 | 0.006871 | 0.026380 | 0.018037 | 0.011401 | 0.008840 |
Mitochondria | 0.036021 | 0.028684 | 0.000181 | 0.003778 | 0.032845 | 0.050974 | 0.001649 | 0.002074 | 0.034802 | 0.049156 | 0.001428 | 0.003313 |
Mitotic Spindle | 0.000739 | 0.002408 | 0.012682 | 0.013302 | 0.002234 | 0.005225 | 0.012918 | 0.006600 | 0.001313 | 0.004995 | 0.012882 | 0.011472 |
None | 0.000382 | 0.000032 | 0.000031 | 0.000045 | 0.000241 | 0.000428 | 0.000143 | 0.000028 | 0.000328 | 0.000396 | 0.000126 | 0.000040 |
Nuclear Periphery | 0.000012 | 0.000088 | 0.000056 | 0.000063 | 0.000053 | 0.000054 | 0.000090 | 0.000036 | 0.000028 | 0.000057 | 0.000085 | 0.000056 |
Nuclear Periphery Foci | 0.000034 | 0.000375 | 0.003160 | 0.000565 | 0.000166 | 0.000160 | 0.001173 | 0.001009 | 0.000085 | 0.000177 | 0.001472 | 0.000686 |
Nucleolus | 0.000065 | 0.000076 | 0.000022 | 0.000071 | 0.000137 | 0.000110 | 0.000089 | 0.000022 | 0.000093 | 0.000107 | 0.000079 | 0.000058 |
Nucleus | 0.003590 | 0.001833 | 0.001616 | 0.003289 | 0.003348 | 0.001182 | 0.010920 | 0.003505 | 0.003498 | 0.001235 | 0.009521 | 0.003348 |
Peroxisomes | 0.350086* | 0.254365* | 0.067073 | 0.213276* | 0.288316* | 0.262155* | 0.246562* | 0.172964* | 0.326363* | 0.261520* | 0.219561* | 0.202273* |
Vacuole | 0.003387 | 0.011727 | 0.046406 | 0.040985 | 0.004960 | 0.007097 | 0.034094 | 0.039587 | 0.003991 | 0.007475 | 0.035947 | 0.040604 |
Vacuole Periphery | 0.000507 | 0.001334 | 0.000596 | 0.000903 | 0.000770 | 0.002060 | 0.000108 | 0.000497 | 0.000608 | 0.002001 | 0.000181 | 0.000793 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.56 | -3.49 | -16.42 | -9.13 | -5.56 | -1.76 | -16.84 | -13.75 | -12.91 | -3.57 | -2.23 | -18.84 | -21.11 | -17.45 | -2.76 |
Bud Neck | 4.28 | 12.92 | 8.00 | 0.80 | -5.53 | 0.60 | 0.77 | 2.78 | 2.30 | 2.23 | 5.49 | 7.01 | 8.07 | 2.08 | 1.02 |
Bud Site | -2.42 | -6.91 | -16.54 | -6.07 | -2.93 | -5.43 | -21.49 | -14.09 | -12.68 | -3.58 | -3.68 | -21.55 | -21.88 | -19.15 | -2.29 |
Cell Periphery | -1.73 | 7.51 | 0.04 | 1.67 | -3.51 | -2.26 | -4.22 | -2.13 | -0.34 | -0.56 | -1.14 | 0.19 | -1.13 | 0.07 | -1.21 |
Cytoplasm | 3.82 | -0.14 | 4.11 | -1.40 | 1.84 | 1.44 | -2.81 | 0.56 | -0.47 | 2.70 | 6.05 | -0.27 | 3.29 | -2.14 | 2.85 |
Cytoplasmic Foci | -3.05 | -6.54 | 17.38 | 8.56 | 12.84 | 4.63 | 22.49 | 21.48 | 18.94 | 3.46 | 1.70 | 17.55 | 25.94 | 20.48 | 5.53 |
Eisosomes | 6.55 | 15.48 | 11.71 | 2.25 | -4.31 | 3.09 | 8.41 | 7.73 | 6.30 | -1.16 | 7.38 | 16.33 | 13.63 | 5.91 | -2.10 |
Endoplasmic Reticulum | -1.80 | -4.51 | -3.87 | -0.83 | -1.63 | -1.43 | -7.02 | -2.43 | -1.99 | 1.25 | -2.47 | -7.75 | -4.51 | -3.25 | 1.16 |
Endosome | -6.27 | -9.44 | -5.18 | 4.11 | 6.57 | -0.32 | 13.30 | 14.54 | 17.04 | 2.32 | -8.28 | 5.22 | 1.65 | 8.32 | -2.46 |
Golgi | -2.04 | 3.30 | 19.08 | 7.15 | 6.18 | -3.91 | 25.99 | 25.92 | 30.71 | 1.08 | -8.92 | 31.16 | 29.40 | 28.24 | -0.92 |
Lipid Particles | 2.61 | -0.62 | 5.85 | 0.85 | 3.39 | 6.57 | 9.98 | 9.46 | 4.43 | 1.41 | 4.46 | 8.96 | 11.20 | 5.16 | 2.07 |
Mitochondria | 1.05 | 17.73 | 15.46 | 3.53 | -5.77 | -4.67 | 12.38 | 11.32 | 15.52 | -0.33 | -4.47 | 20.99 | 19.16 | 16.22 | -3.04 |
Mitotic Spindle | -1.62 | -3.50 | -6.83 | -5.42 | -0.41 | -3.26 | -6.06 | -2.45 | -1.11 | 2.06 | -4.65 | -7.31 | -7.12 | -4.37 | 0.51 |
None | 3.09 | 3.01 | 2.84 | -2.44 | -1.39 | -0.68 | 0.66 | 3.14 | 1.45 | 4.45 | -0.26 | 1.98 | 3.68 | 1.40 | 4.01 |
Nuclear Periphery | -1.66 | -4.39 | -6.92 | -0.09 | -0.41 | 0.02 | -3.90 | -1.42 | -1.50 | 1.12 | -1.75 | -7.64 | -4.95 | -2.17 | 2.19 |
Nuclear Periphery Foci | -2.25 | -4.38 | -4.70 | -1.75 | 3.52 | 0.24 | -5.13 | -1.97 | -2.01 | 0.78 | -1.80 | -6.83 | -4.25 | -3.75 | 3.45 |
Nucleolus | -0.31 | 2.13 | -0.55 | -0.04 | -1.86 | 0.66 | 0.60 | 3.59 | 1.91 | 2.96 | -0.31 | -0.37 | 1.16 | 0.95 | 1.24 |
Nucleus | 2.34 | 1.27 | -1.65 | -3.30 | -2.32 | 4.95 | -7.32 | -2.39 | -4.49 | 4.74 | 7.47 | -7.20 | -2.79 | -7.50 | 5.83 |
Peroxisomes | 4.35 | 32.60* | 15.68 | 2.34 | -14.05 | 2.73 | 6.08 | 9.80 | 7.95 | 5.29 | 8.51 | 16.35 | 17.68 | 8.01 | 1.47 |
Vacuole | -2.78 | -7.40 | -13.67 | -9.53 | -0.27 | -2.05 | -13.71 | -8.14 | -7.86 | -1.56 | -4.04 | -15.75 | -15.83 | -14.68 | -1.59 |
Vacuole Periphery | -2.73 | -0.78 | -1.50 | 1.33 | -0.93 | -5.10 | 6.58 | 0.89 | 3.95 | -1.07 | -6.31 | 8.03 | -0.68 | 4.50 | -3.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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