Standard name
Human Ortholog
Description Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0.05 0 0 0 – 0 0 0 0 – 0 0.07 0 0 0 0.1 0.1 0.11 0 0.11 0.08
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 – 0.1 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.06 0.1 0.13 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.26 0.32 0.59 0.44 0.47 0.15 0.17 – 0.16 0.17 0.08 0.17 – 0.65 0.69 0.6 0.45 0.52 0.43 0.43 0.26 0.35 0.46 0.45
Mitochondria 0.72 0.57 0.4 0.78 0.64 0.79 0.78 – 0.8 0.86 0.92 0.81 – 0.42 0.58 0.26 0.31 0.23 0.35 0.31 0.47 0.32 0.29 0.38
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0
Nucleolus 0.26 0.25 0.1 0.1 0.13 0.22 0.24 – 0.16 0.15 0.17 0.17 – 0.1 0.05 0.17 0.16 0.19 0 0 0 0.05 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.16 0.19 0 0.1 0.13 0.09 – 0.07 0.09 0.05 0.06 – 0.09 0 0.1 0.11 0.08 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 1 9 8 6 2 7 – 7 1 1 7 – 2 4 0 0 0 47 31 22 1 7 5
Bud 3 1 0 1 0 1 2 – 0 1 0 4 – 0 0 0 1 0 1 0 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 3 6 4 2 1 1 2 – 0 0 0 1 – 0 0 10 8 9 0 1 0 0 0 0
Cytoplasm 1 3 3 1 5 4 4 – 4 4 1 2 – 8 2 8 12 9 0 0 0 0 0 0
Endoplasmic Reticulum 7 1 0 0 0 0 1 – 1 0 1 1 – 0 1 19 29 29 2 1 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 4 4 5 0 0 0
Golgi 99 79 106 65 93 37 44 – 40 30 16 37 – 53 38 196 125 119 193 137 54 13 32 30
Mitochondria 279 139 73 114 126 201 204 – 198 150 176 182 – 34 32 85 86 53 157 99 99 12 20 26
Nucleus 1 2 0 0 0 0 2 – 0 0 0 0 – 0 0 2 4 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 – 1 1 3 0 – 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 101 60 19 14 25 56 62 – 40 26 33 39 – 8 3 54 44 43 9 9 4 1 3 1
Peroxisomes 0 0 2 2 2 0 0 – 0 0 0 1 – 0 0 0 1 0 17 11 13 0 1 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0
Vac/Vac Membrane 36 38 34 4 19 32 24 – 18 15 9 14 – 7 2 32 31 18 4 7 3 2 0 1
Unique Cell Count 387 244 181 147 198 255 261 249 174 192 224 81 55 326 280 230 452 319 210 38 71 69
Labelled Cell Count 537 330 250 211 277 334 356 309 228 240 288 112 82 406 341 282 452 319 210 38 71 69


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 80.0 103.3 67.7 67.4 64.7 59.7 50.3 – 46.3 43.7 42.8 43.6 – 64.5 58.6 142.1 152.4 155.5 – 62.6 63.8
Std Deviation (1e-4) 20.1 25.9 20.5 17.9 19.8 16.4 20.0 – 13.4 14.7 12.4 13.7 – 22.5 16.9 40.0 44.2 42.6 – 21.5 19.2
Intensity Change (Log2) – – – -0.01 -0.07 -0.18 -0.43 – -0.55 -0.63 -0.66 -0.63 – -0.07 -0.21 1.07 1.17 1.2 – -0.11 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -1.0 0 -1.3 – -1.2 0 0 -0.9 – 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 1.0
Cytoplasm 0 0 0 0 – 0 0 0 0 – 0 0 0 1.6 1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 3.3 4.5 5.0
Endosome 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Golgi -2.6 -2.3 -9.7 -9.1 – -9.2 -8.0 -10.3 -8.8 – 1.1 1.4 0.3 -2.9 -1.4
Mitochondria 6.8 4.5 8.2 8.1 – 8.3 8.9 10.5 8.5 – 0.3 2.3 -3.3 -2.1 -3.8
Nucleus 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleolus -0.3 0.6 3.1 3.5 – 1.7 1.3 1.9 2.0 – -0.2 -1.1 1.9 1.6 2.3
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Vacuole -4.5 -2.6 -1.8 -2.9 – -3.6 -2.8 -4.3 -3.9 – -2.1 -2.7 -2.9 -2.3 -3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 99.9994 114.9235 109.0189 96.51 101.2685 107.102 121.0375 126.886 125.6926 89.897 109.2972 118.571 148.0646 138.9156 136.5635 126.8661 123.9034 130.1539
Actin 0.0176 0.0083 0.0051 0.0432 0.0007 0.0007 0.0185 0.0059 0.003 0.0465 0.0277 0.0047 0.0021 0.0074 0.0175 0.0013 0.038 0.0023
Bud 0.0002 0.0002 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0004 0.0001 0 0.0003 0 0.0001 0 0 0
Bud Neck 0.0009 0.0005 0.0002 0.0003 0 0 0.0005 0.0018 0.0001 0.0023 0.0006 0.0003 0.0001 0.0001 0.0004 0 0.0001 0.0002
Bud Periphery 0.0002 0.0003 0.0001 0.0001 0.0001 0 0.0002 0.0003 0.0001 0.0008 0.0002 0.0001 0.0002 0 0.0002 0 0.0001 0
Bud Site 0.0009 0.0011 0.0002 0.0008 0.0002 0 0.0006 0.0027 0.0003 0.0023 0.0003 0.0001 0.0002 0.0001 0.001 0 0.0001 0.0001
Cell Periphery 0.0005 0.003 0.0018 0.0013 0.0005 0.0006 0.0014 0.001 0.0008 0.0024 0.0006 0.0008 0.0004 0.0004 0.0003 0.0001 0.0003 0.0002
Cytoplasm 0.0011 0.0001 0.0001 0 0.0001 0.0166 0.001 0 0 0.0054 0.0001 0 0.0006 0 0.0001 0 0 0
Cytoplasmic Foci 0.0189 0.0012 0.0038 0.0002 0.0043 0.008 0.0038 0.0008 0.0003 0.0159 0.0007 0.0002 0.001 0.0001 0.0043 0 0.001 0.0048
Eisosomes 0.0101 0.0086 0.0108 0.0026 0.0025 0.0011 0.0188 0.0081 0.0115 0.0083 0.0108 0.0028 0.0152 0.0028 0.0177 0.0011 0.0043 0.0043
Endoplasmic Reticulum 0.0011 0 0 0 0 0.0003 0.0019 0 0 0.004 0.0004 0.0001 0.0001 0 0.0001 0 0 0
Endosome 0.0115 0.0012 0.0004 0.0002 0.0002 0.0026 0.0026 0.0007 0.0001 0.0257 0.0015 0.0004 0.0002 0.0002 0.0017 0 0.0003 0.0015
Golgi 0.0106 0.0068 0.0025 0.0011 0.001 0.0104 0.0046 0.0027 0.001 0.0177 0.0039 0.0111 0.0028 0.0048 0.0023 0 0.0018 0.0031
Lipid Particles 0.0242 0.0194 0.0165 0.0162 0.0215 0.0154 0.0066 0.0083 0.0028 0.053 0.0062 0.0081 0.0026 0.007 0.0097 0.0008 0.0174 0.0023
Mitochondria 0.844 0.9211 0.9186 0.9022 0.9346 0.8702 0.8982 0.9443 0.9631 0.7083 0.9212 0.9485 0.9577 0.9557 0.9226 0.9944 0.8912 0.9719
None 0.0089 0 0.001 0 0.001 0.0002 0.0023 0 0.0001 0.0108 0.0007 0 0.0003 0 0.0005 0 0.0002 0
Nuclear Periphery 0.0041 0.0001 0.0003 0.0001 0.0003 0.0006 0.0037 0.0001 0.0001 0.0127 0.0026 0.0005 0.0006 0.0001 0.0004 0 0.0002 0.0001
Nucleolus 0.0206 0.0019 0.0077 0.0016 0.0026 0.0093 0.0138 0.0044 0.0051 0.0142 0.0007 0.0036 0.007 0.0006 0.0037 0.0002 0.0294 0.0001
Nucleus 0.0024 0.0003 0.0005 0.0002 0.0003 0.0004 0.0018 0.0004 0.0004 0.0073 0.0004 0.0003 0.0018 0.0001 0.0002 0 0.0009 0
Peroxisomes 0.0053 0.0042 0.0039 0.0014 0.0067 0.0009 0.005 0.0064 0.0016 0.0075 0.0026 0.001 0.0022 0.0013 0.0104 0.0013 0.0026 0.0027
Punctate Nuclear 0.0035 0 0.0001 0 0 0 0.0055 0 0 0.0156 0.0027 0 0.0001 0 0.0007 0 0.0003 0.0001
Vacuole 0.0019 0.004 0.0021 0.0016 0.001 0.0027 0.0015 0.0018 0.0008 0.0052 0.0008 0.0006 0.0004 0.0009 0.0005 0.0001 0.0008 0.0003
Vacuole Periphery 0.0117 0.0177 0.0243 0.0266 0.0223 0.0597 0.0077 0.01 0.0087 0.0338 0.0152 0.017 0.0042 0.0182 0.0054 0.0006 0.0109 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 186.5703 152.7292 123.9565 116.2126 144.5995 203.9575 182.8427 153.2629 163.971 203.4264
Translational Efficiency 1.2165 1.1107 0.9846 1.2723 1.2403 1.2733 1.0662 1.2061 1.1465 1.0799

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
338 1327 275 2343 828 911 237 1814 1166 2238 512 4157

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3386.13 5491.74 9929.70 3336.80 7022.61 5745.59 5376.52 3438.57 5968.47 5595.07 7822.08 3381.21
Standard Deviation 931.10 1594.55 1837.56 826.64 1690.41 1643.44 1002.67 963.39 2236.65 1619.44 2726.41 890.34
Intensity Change Log 2 — 0.697626 1.552113 -0.021172 — -0.289552 -0.385335 -1.030199 — 0.110504 0.556323 -0.619424

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.011003 0.012476 0.023128 0.006148 0.012738 0.013539 0.021402 0.012209 0.012235 0.012909 0.022329 0.008793
Bud Neck 0.002328 0.004865 0.012881 0.000628 0.003835 0.003284 0.025307 0.001334 0.003398 0.004221 0.018633 0.000936
Bud Site 0.001265 0.006658 0.004634 0.000771 0.003301 0.005976 0.018676 0.001829 0.002711 0.006380 0.011134 0.001233
Cell Periphery 0.000259 0.000562 0.000394 0.000270 0.000331 0.000279 0.000970 0.000339 0.000310 0.000447 0.000661 0.000300
Cytoplasm 0.000314 0.001109 0.000355 0.000246 0.000692 0.000946 0.001332 0.000455 0.000583 0.001042 0.000807 0.000337
Cytoplasmic Foci 0.015558 0.040017 0.118927 0.003961 0.080051 0.045181 0.203579* 0.009675 0.061356 0.042119 0.158111 0.006455
Eisosomes 0.008133 0.006928 0.003049 0.001415 0.003609 0.002214 0.001988 0.002014 0.004920 0.005009 0.002558 0.001676
Endoplasmic Reticulum 0.000139 0.000178 0.000291 0.000063 0.000205 0.000214 0.000482 0.000273 0.000186 0.000193 0.000379 0.000155
Endosome 0.000490 0.001361 0.002062 0.000483 0.004565 0.004712 0.008627 0.001077 0.003384 0.002725 0.005101 0.000742
Golgi 0.016302 0.019425 0.070435 0.011684 0.064173 0.061480 0.081187 0.025896 0.050296 0.036544 0.075412 0.017886
Lipid Particles 0.005508 0.007434 0.033556 0.001382 0.022256 0.024867 0.024029 0.004376 0.017401 0.014530 0.029146 0.002689
Mitochondria 0.887915* 0.847450* 0.521810* 0.957572* 0.724202* 0.769575* 0.391723* 0.907931* 0.771659* 0.815750* 0.461594* 0.935910*
Mitotic Spindle 0.000834 0.000540 0.008785 0.000630 0.001356 0.001230 0.018153 0.001408 0.001204 0.000821 0.013121 0.000969
None 0.011429 0.004283 0.009632 0.002147 0.005699 0.004620 0.004551 0.003614 0.007360 0.004420 0.007280 0.002788
Nuclear Periphery 0.000583 0.000268 0.011027 0.000160 0.001101 0.001274 0.006850 0.000442 0.000951 0.000678 0.009093 0.000283
Nuclear Periphery Foci 0.000201 0.000308 0.002498 0.000052 0.000730 0.000540 0.002656 0.000200 0.000577 0.000403 0.002571 0.000117
Nucleolus 0.001907 0.003431 0.006326 0.000318 0.002786 0.002296 0.008952 0.000466 0.002531 0.002969 0.007541 0.000382
Nucleus 0.001460 0.000420 0.000271 0.000079 0.000184 0.000755 0.000627 0.000088 0.000554 0.000556 0.000436 0.000083
Peroxisomes 0.012305 0.021195 0.010082 0.003050 0.010299 0.013202 0.019735 0.006233 0.010880 0.017941 0.014550 0.004439
Vacuole 0.005556 0.009265 0.037476 0.001722 0.019760 0.015442 0.067234 0.004311 0.015642 0.011780 0.051251 0.002852
Vacuole Periphery 0.016510 0.011830 0.122381* 0.007220 0.038127 0.028376 0.091942 0.015830 0.031861 0.018565 0.108291* 0.010977

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.46 -2.38 1.58 3.96 3.99 -0.32 -2.31 0.25 0.59 2.58 -0.36 -3.24 2.02 3.02 4.82
Bud Neck -2.71 -6.40 2.43 6.30 8.06 1.05 -8.52 6.21 5.04 9.57 -1.55 -10.34 7.10 7.67 12.23
Bud Site -4.27 -4.84 0.68 4.60 5.62 -1.50 -6.30 1.78 2.51 7.12 -3.28 -6.55 2.35 5.07 8.18
Cell Periphery -1.59 -2.03 -0.10 1.44 1.11 0.67 -3.22 -0.28 -1.08 3.16 -1.22 -3.44 0.05 1.20 3.27
Cytoplasm -3.64 -0.31 -0.17 3.27 0.04 -1.27 -3.08 0.83 2.13 3.85 -2.87 -1.86 1.07 3.88 3.04
Cytoplasmic Foci -6.07 -8.15 4.12 12.10 9.27 5.19 -7.09 12.15 9.69 11.44 4.02 -8.73 12.96 15.39 14.50
Eisosomes 0.84 3.82 5.33 7.79 3.50 2.83 3.61 3.48 0.50 -0.16 -0.14 4.48 6.81 7.34 2.88
Endoplasmic Reticulum -1.31 -3.56 2.96 5.67 6.29 -0.24 -1.93 -1.14 -0.99 1.34 -0.29 -2.72 0.90 1.17 3.02
Endosome -4.15 -5.72 -0.01 4.63 6.08 -0.18 -2.82 6.25 6.36 5.60 1.42 -2.28 6.60 7.60 6.65
Golgi -0.82 -6.80 1.31 3.67 8.05 0.43 -1.59 7.64 7.25 5.94 3.41 -3.72 9.00 7.73 9.90
Lipid Particles -1.23 -5.11 2.93 7.71 6.05 -0.83 -0.53 8.72 8.33 6.43 1.58 -3.37 9.78 10.56 8.30
Mitochondria 3.20 15.97 -6.28 -14.79 -21.28* -2.94 13.49 -14.76 -12.47 -22.90* -4.07 17.63 -17.48 -18.78 -30.74*
Mitotic Spindle 0.74 -6.05 0.44 -0.48 6.39 0.30 -5.43 -0.15 -0.39 5.37 1.43 -7.40 0.65 -0.65 7.54
None 4.75 1.03 6.21 9.19 7.60 2.60 2.14 5.61 3.34 1.66 5.64 0.18 9.02 8.79 7.55
Nuclear Periphery 2.41 -3.68 3.25 3.41 3.84 -0.58 -5.32 4.51 3.11 5.98 1.83 -5.06 6.22 3.55 5.49
Nuclear Periphery Foci -1.41 -5.84 3.70 3.85 6.24 1.47 -4.85 3.39 2.84 6.11 1.83 -6.97 4.83 4.44 8.76
Nucleolus -1.82 -4.16 2.44 5.86 7.12 0.84 -1.83 5.81 4.29 2.54 -0.90 -3.05 6.29 7.19 4.44
Nucleus 0.76 0.87 1.00 4.17 3.82 -1.16 -3.41 1.89 1.31 3.92 -0.04 0.26 1.15 2.19 5.01
Peroxisomes -2.16 0.53 2.51 9.12 3.73 -1.28 -2.30 2.84 3.17 3.35 -3.69 -1.48 4.59 9.08 4.77
Vacuole -2.22 -9.41 2.96 6.37 11.25 1.60 -7.32 6.85 6.10 10.11 2.09 -9.57 7.91 8.69 14.15
Vacuole Periphery 1.45 -10.13 3.06 3.05 11.44 2.39 -5.56 6.33 4.53 8.37 4.75 -10.47 8.04 5.20 14.15
Standard name
Human Ortholog
Description Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Leu4

Leu4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Leu4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available