Standard name
Human Ortholog
Description Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.06 0 0.1 0 0 0.05 0 0.06 0.12 0.13 0.12 0.12 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.26 0.52 0.22 0.3 0.36 0.28 0.5 0.52 0.59 0.59 0 0 0.14 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.88 0.9 0.82 0.8 0.77 0.78 0.71 0.73 0.73 0.69 0.72 0.91 0.83 0.76 0.76 0.69 0.67 0.84 0.84 0.84 0.9 0.75 0.74
Nuclear Periphery 0 0.07 0.05 0 0.12 0.09 0.1 0.05 0.06 0.06 0.06 0.09 0 0 0 0.09 0.09 0.05 0.07 0 0 0 0 0
Nucleolus 0.07 0.05 0 0.05 0 0.05 0.05 0.05 0.09 0.09 0 0.09 0 0.05 0.1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0 0.07 0.08 0 0.08 0 0 0 0 0 0 0 0.11 0.16 0.1 0 0.05 0.08 0 0.14 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 2 1 7 9 4 4 0 0 0 1 0 0 1 3 1 0 4 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 1 1 0 2 12 9 20 23 10 25 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 4 10 18 22 20 47 20 24 14 16 11 28 32 11 8 12 0 1 0 0 2 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 1 1 0 0 0 1 0 1 0 2 0 0 0 0 1 1
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 1 0 3 1 1 1 3
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Mitochondria 2 17 50 97 84 118 169 128 238 340 165 275 4 4 35 3 1 3 1 4 0 0 2 4
Nucleus 137 231 171 154 302 301 365 327 351 476 195 337 162 192 192 67 44 41 126 228 156 127 163 182
Nuclear Periphery 5 18 10 2 46 34 47 23 31 40 18 40 3 1 4 8 6 3 10 4 4 2 1 0
Nucleolus 10 12 7 9 15 18 21 22 43 60 6 40 7 12 24 0 0 0 4 9 5 1 2 7
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 2 2 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 1 1 0 2 3 2 2 0 0 0 1 1 0 0 0 0 2 0 0 4 2
Vac/Vac Membrane 6 30 6 5 25 33 13 39 10 12 5 13 3 4 8 10 10 6 4 12 15 4 31 37
Unique Cell Count 146 263 189 187 377 391 466 461 478 652 281 470 179 232 251 88 64 61 150 273 187 142 218 248
Labelled Cell Count 164 312 249 281 491 529 651 600 724 987 417 750 190 246 300 101 70 68 150 273 187 142 218 248


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.7 4.3 4.4 4.7 4.6 4.3 5.4 4.0 3.9 4.1 3.6 6.4 6.4 5.4 7.8 8.9 8.9 6.0 5.4 6.0
Std Deviation (1e-4) 1.3 1.1 0.8 1.7 1.4 1.5 1.2 3.2 1.5 1.2 1.6 1.0 1.5 1.7 1.4 1.9 2.4 2.3 1.6 1.5 1.3
Intensity Change (Log2) – – – 0.01 0.13 0.08 -0.03 0.32 -0.1 -0.13 -0.07 -0.26 0.57 0.56 0.31 0.85 1.05 1.05 0.46 0.32 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.5 1.9 1.3 3.5 1.3 1.1 1.6 0.9 2.0 3.8 4.0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.3 0 0 0 0 -6.6 -7.5 -3.3 -4.5 -4.3 -3.6
Nucleus -2.3 -3.1 -3.9 -3.7 -5.3 -4.8 -5.0 -5.4 -5.2 0 -2.3 -3.8 -3.2 -4.2 -4.4
Nuclear Periphery 0 0 0 0 -0.2 0 0 0 0 -1.9 0 -2.2 1.2 0 0
Nucleolus 0 0 0 0 0.6 0 0 0 0 0.1 0.7 2.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 1.7 2.4 -0.3 2.4 0 0 0 -0.3 0 0 0 2.7 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.652 5.9307 5.7789 4.5722 4.5844 4.9839 4.5142 5.7837 5.9757 5.2017 4.4622 4.6344 3.0933 3.4619 3.4957 3.7453 2.8264 3.1764
Actin 0.0403 0.0018 0.002 0.0011 0.0156 0.0006 0.0066 0 0.0009 0.0001 0.0025 0.0007 0 0 0.0002 0.0001 0.0015 0
Bud 0.0009 0.0001 0.0002 0.0001 0.0002 0.0002 0.0012 0 0 0 0.0002 0.0001 0 0.0001 0 0 0.0002 0
Bud Neck 0.0027 0.0003 0.0002 0.0002 0.0008 0.0023 0.0014 0 0 0 0.0061 0.0007 0 0.0001 0.0001 0 0.0002 0.0004
Bud Periphery 0.0027 0.0002 0.0004 0.0002 0.0004 0.0011 0.003 0 0 0 0.0004 0.0004 0 0.0001 0 0 0.0005 0.0001
Bud Site 0.0034 0.0171 0.0012 0.0004 0.0003 0.0003 0.0039 0 0 0 0.0033 0.0001 0 0.0001 0.0002 0 0.0004 0
Cell Periphery 0.0009 0.0001 0.0002 0.0001 0.0001 0.0004 0.0009 0 0 0 0.0005 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0035 0.0002 0.0008 0.0005 0.0005 0.0006 0.0121 0 0.0002 0.0001 0.0023 0.0002 0 0.0007 0.0004 0.001 0.0003 0.0001
Cytoplasmic Foci 0.0078 0.0006 0.007 0.0038 0.0012 0.002 0.0235 0 0.0015 0 0.007 0.0015 0 0 0.0121 0 0.0055 0
Eisosomes 0.0005 0 0.0002 0 0.0002 0 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0042 0 0.0031 0.0017 0.0015 0.0015 0.0045 0 0.0002 0.0001 0.0043 0.0003 0 0.0001 0.0004 0.0005 0.0011 0.0001
Endosome 0.0156 0.0001 0.0055 0.0063 0.014 0.0033 0.0177 0 0.0034 0 0.0318 0.0033 0 0.0002 0.0013 0.0001 0.0053 0
Golgi 0.006 0.0002 0.0028 0.0024 0.0214 0.0005 0.0028 0 0.0009 0 0.006 0.0013 0 0 0.0003 0 0.007 0
Lipid Particles 0.0196 0 0.0136 0.0339 0.0054 0.0051 0.0106 0 0.0026 0 0.0206 0.0043 0 0 0.0064 0.0001 0.0085 0
Mitochondria 0.0282 0.0124 0.0255 0.0035 0.0109 0.0111 0.0141 0.0003 0.0004 0.0003 0.002 0.0021 0.0001 0.0015 0.0006 0.0042 0.0492 0.0002
None 0.0035 0 0.001 0.0002 0.0002 0.0002 0.0049 0 0.0002 0 0.0006 0.0001 0 0.0001 0.0003 0.0014 0.0001 0
Nuclear Periphery 0.0613 0.0105 0.0241 0.4004 0.3593 0.0568 0.0356 0.0128 0.014 0.1382 0.2076 0.0889 0.0015 0.0214 0.0582 0.3194 0.5428 0.0463
Nucleolus 0.0226 0.0166 0.0141 0.0039 0.0071 0.0263 0.0154 0.0082 0.0055 0.0013 0.0053 0.0106 0.0061 0.008 0.0063 0.0004 0.0037 0.0192
Nucleus 0.7384 0.9386 0.884 0.5267 0.5504 0.8773 0.7946 0.9778 0.9669 0.8586 0.6828 0.8768 0.9922 0.9656 0.9034 0.6635 0.3545 0.9325
Peroxisomes 0.003 0.0002 0.0029 0.0025 0.0004 0.0005 0.0035 0 0.0007 0 0.0011 0.0028 0 0 0.0005 0 0.0074 0
Punctate Nuclear 0.0189 0.0003 0.0082 0.0031 0.0007 0.0017 0.0351 0.0001 0.0021 0.0001 0.0049 0.0009 0 0.0003 0.0078 0.0004 0.0008 0.0002
Vacuole 0.0089 0.0002 0.0012 0.001 0.0014 0.0038 0.0045 0.0001 0.0001 0.0001 0.0061 0.0008 0 0.0007 0.0007 0.0002 0.0013 0.0003
Vacuole Periphery 0.0072 0.0003 0.002 0.0081 0.008 0.0044 0.0039 0.0004 0.0003 0.0011 0.0044 0.0038 0 0.0009 0.0008 0.0088 0.0096 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.5137 22.1582 9.1346 27.0266 70.2808 67.0635 18.557 12.7538 19.9323 75.8339
Translational Efficiency 1.1358 0.8872 0.8148 0.7246 0.8669 1.3695 0.901 0.6976 0.6019 0.7012

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
723 167 241 1114 1342 1747 152 614 2065 1914 393 1728

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 619.99 926.03 1192.63 1039.97 673.63 857.36 1129.75 1027.73 654.85 863.35 1168.31 1035.62
Standard Deviation 123.00 164.63 165.92 156.51 137.47 126.02 128.29 161.69 135.03 131.28 155.52 158.48
Intensity Change Log 2 — 0.578814 0.943830 0.746225 — 0.347945 0.745975 0.609433 — 0.463208 0.844190 0.676613

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000280 0.000808 0.001227 0.000850 0.000426 0.000352 0.000888 0.000880 0.000375 0.000391 0.001096 0.000860
Bud Neck 0.003795 0.007363 0.011945 0.029243 0.003497 0.006015 0.011416 0.017850 0.003602 0.006132 0.011740 0.025195
Bud Site 0.001821 0.001051 0.000902 0.006815 0.000810 0.000764 0.000766 0.002436 0.001164 0.000789 0.000850 0.005259
Cell Periphery 0.000219 0.000065 0.000053 0.000116 0.000312 0.000111 0.000042 0.000127 0.000279 0.000107 0.000049 0.000120
Cytoplasm 0.022713 0.002449 0.000573 0.011545 0.016201 0.002230 0.001141 0.009971 0.018481 0.002249 0.000793 0.010986
Cytoplasmic Foci 0.002367 0.002156 0.000037 0.002953 0.001509 0.000737 0.000049 0.002871 0.001810 0.000861 0.000042 0.002924
Eisosomes 0.000020 0.000031 0.000043 0.000019 0.000019 0.000018 0.000028 0.000020 0.000020 0.000019 0.000037 0.000019
Endoplasmic Reticulum 0.005430 0.012153 0.009181 0.011952 0.008908 0.009556 0.013475 0.011595 0.007691 0.009782 0.010842 0.011826
Endosome 0.000946 0.000857 0.000217 0.007673 0.000678 0.000816 0.000374 0.005074 0.000772 0.000820 0.000278 0.006749
Golgi 0.000655 0.002454 0.000071 0.005456 0.000211 0.000462 0.000076 0.002006 0.000366 0.000636 0.000073 0.004230
Lipid Particles 0.000937 0.002647 0.000115 0.000375 0.000340 0.000406 0.000116 0.000240 0.000549 0.000602 0.000115 0.000327
Mitochondria 0.001998 0.002233 0.002484 0.009396 0.001247 0.002985 0.002061 0.004869 0.001510 0.002919 0.002320 0.007788
Mitotic Spindle 0.003216 0.009941 0.000086 0.032706 0.000244 0.004374 0.001191 0.022268 0.001285 0.004860 0.000514 0.028997
None 0.035167 0.001736 0.000618 0.002520 0.035830 0.001614 0.001353 0.003845 0.035598 0.001625 0.000902 0.002991
Nuclear Periphery 0.007329 0.013591 0.016870 0.186997* 0.013200 0.022082 0.113448* 0.154126* 0.011145 0.021342 0.054224 0.175317*
Nuclear Periphery Foci 0.002947 0.002170 0.000750 0.028129 0.002309 0.002859 0.005996 0.028179 0.002532 0.002799 0.002779 0.028147
Nucleolus 0.031886 0.024665 0.010742 0.023918 0.028644 0.019540 0.023365 0.033062 0.029779 0.019988 0.015624 0.027167
Nucleus 0.875712* 0.907146* 0.940348* 0.622763* 0.883771* 0.921587* 0.819479* 0.687340* 0.880949* 0.920327* 0.893600* 0.645709*
Peroxisomes 0.000365 0.002811 0.000486 0.000499 0.000495 0.000835 0.000399 0.000417 0.000450 0.001007 0.000452 0.000470
Vacuole 0.001580 0.003274 0.002728 0.009958 0.001037 0.001821 0.003103 0.008154 0.001227 0.001948 0.002873 0.009317
Vacuole Periphery 0.000618 0.000398 0.000523 0.006116 0.000309 0.000835 0.001233 0.004669 0.000417 0.000797 0.000798 0.005602

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.50 -12.11 -13.68 -2.03 3.59 1.77 -6.91 -8.55 -9.62 -1.24 -0.26 -13.25 -14.97 -14.55 2.17
Bud Neck -2.62 -4.97 -12.83 -10.35 -8.60 -3.97 -4.39 -10.12 -8.38 -1.82 -4.06 -6.83 -15.97 -14.03 -8.04
Bud Site 0.46 1.51 -3.42 -3.62 -4.71 0.29 -1.51 -4.39 -4.45 -3.24 1.49 0.04 -5.39 -6.05 -5.54
Cell Periphery 3.23 3.49 2.24 -3.26 -5.26 5.56 7.87 4.80 -0.69 -3.70 5.71 8.36 5.70 -0.69 -6.28
Cytoplasm 5.23 7.91 4.05 -2.63 -9.76 7.13 7.48 1.91 -5.92 -6.35 9.63 11.15 4.24 -8.29 -11.46
Cytoplasmic Foci 0.14 4.37 1.03 0.37 -3.26 1.34 3.54 -0.52 -1.28 -2.23 1.90 5.36 0.11 -1.47 -3.86
Eisosomes -2.15 -7.53 -2.80 1.09 6.31 0.96 -4.58 -3.60 -4.05 0.42 0.63 -8.98 -4.99 -5.19 5.38
Endoplasmic Reticulum -3.11 -2.66 -4.36 1.00 -1.46 -0.78 -1.56 -1.60 -1.06 0.78 -2.83 -2.36 -3.99 -1.20 -0.30
Endosome 0.00 4.06 -3.71 -3.53 -4.55 -0.66 0.85 -2.71 -2.58 -2.83 -0.21 4.16 -4.72 -4.61 -5.35
Golgi -0.79 1.33 -2.33 -0.32 -3.11 -1.24 1.27 -1.90 -1.43 -2.11 -0.91 1.74 -3.12 -2.62 -3.62
Lipid Particles -0.89 4.43 3.06 1.21 -2.17 -0.90 3.37 -0.36 0.67 -3.78 -0.43 5.58 2.23 1.46 -3.36
Mitochondria -0.30 -0.73 -4.10 -3.80 -4.04 -2.37 -3.70 -7.22 -1.75 -4.96 -2.05 -3.16 -5.91 -3.29 -4.98
Mitotic Spindle -1.07 2.22 -6.99 -2.59 -8.53 -3.36 -1.81 -5.77 -4.80 -5.37 -2.56 0.99 -9.70 -7.98 -10.02
None 7.28 7.56 7.06 -2.49 -9.56 10.10 10.07 8.98 -4.16 -3.85 12.46 12.73 11.73 -5.04 -7.53
Nuclear Periphery -0.81 -1.14 -13.75 -10.20 -11.63 -2.50 -3.86 -8.84 -7.90 -0.72 -3.37 -3.70 -15.97 -13.80 -7.00
Nuclear Periphery Foci -0.06 2.33 -5.90 -5.13 -7.81 -0.66 -1.92 -6.13 -5.82 -4.83 -0.38 0.45 -8.86 -8.33 -8.91
Nucleolus 2.00 6.62 2.92 0.28 -4.54 3.68 1.49 -1.53 -3.71 -2.35 4.78 6.09 1.27 -2.91 -4.48
Nucleus -1.35 -6.80 12.20 9.92 18.90 -5.19 1.82 10.25 13.11 4.31 -6.18 -2.35 17.00 21.49 14.69
Peroxisomes -1.45 -2.01 -0.15 1.44 1.71 -0.73 0.21 0.32 0.85 0.10 -1.23 -0.55 0.47 1.36 1.40
Vacuole -1.47 -3.28 -6.90 -0.98 -5.71 -1.65 -2.65 -6.70 -6.00 -2.94 -1.88 -4.26 -9.46 -7.76 -6.24
Vacuole Periphery 1.07 0.87 -4.12 -4.47 -4.41 -3.21 -2.32 -4.21 -3.32 -2.95 -2.38 -1.49 -5.83 -4.94 -5.24
Standard name
Human Ortholog
Description Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pol1

Pol1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available