Standard name
Human Ortholog
Description Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.98 0.98 0.94 0.82 0.72 0.65 0.68 0.58 0.58 0.55 0.99 0.98 0.99 0.98 0.97 0.95 0.93 0.92 0.91 0.84 0.83 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.11 0.2 0.38 0.56 0.47 0.56 0.56 0.62 0.63 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 3 2 0 4 2 0 0 0 0 0 0 1 3 6
Bud 0 3 0 1 9 17 12 20 12 11 17 24 2 1 0 0 0 1 0 0 0 1 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 2 2 1 3
Bud Site 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 1 3 9 4 8 10 11 11 19 4 12 16 2 10 10 0 0 0 0 0 1
Cytoplasm 46 263 211 343 569 525 561 600 439 513 313 521 483 556 741 87 96 134 77 258 200 299 329 448
Endoplasmic Reticulum 0 4 1 0 2 6 7 14 9 7 7 18 4 6 4 7 5 8 0 1 4 12 13 28
Endosome 0 0 0 0 4 2 0 7 0 3 1 1 1 0 2 0 0 0 0 2 1 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 3 4
Mitochondria 5 8 9 37 118 245 436 432 363 497 337 596 0 0 1 4 2 3 1 6 4 10 12 16
Nucleus 0 1 0 0 3 0 2 7 0 2 1 5 2 0 2 2 2 1 0 0 0 0 0 2
Nuclear Periphery 0 1 0 0 0 0 2 6 0 3 4 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 3 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 0 1 0 2 2 5 3 28 22 43 15 23 4 2 2 2 0 1 0 4 1 22 19 27
Unique Cell Count 47 273 216 351 604 637 784 927 644 882 543 952 489 566 750 89 99 141 84 280 220 358 396 558
Labelled Cell Count 52 282 222 384 710 809 1028 1126 855 1092 710 1213 500 582 773 104 115 158 84 280 220 358 396 558


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.0 4.9 5.0 4.8 4.8 4.1 4.6 4.4 4.4 4.8 4.6 7.2 7.1 7.2 7.4 7.7 7.7 7.7 7.8 8.1
Std Deviation (1e-4) 0.5 1.1 0.6 0.8 0.9 1.0 0.9 1.0 0.9 0.8 0.9 0.8 0.9 0.9 0.9 1.3 1.3 1.2 1.1 1.4 1.7
Intensity Change (Log2) – – – 0.04 -0.02 -0.03 -0.25 -0.07 -0.14 -0.15 -0.03 -0.08 0.57 0.56 0.56 0.62 0.66 0.68 0.66 0.69 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -2.0 -5.6 -8.1 -9.6 -8.7 -11.0 -10.8 -11.8 1.1 0.5 1.2 0 -0.4 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 2.8 3.3 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.47 4.4665 4.0747 3.7315 3.3589 4.0435 2.9832 4.2948 3.7693 3.2 3.769 4.0637 1.2815 2.4381 2.2842 1.8956 1.943 1.9234
Actin 0.0005 0.0007 0.0008 0.0003 0.0001 0.0004 0.0215 0.0037 0.0009 0.0001 0.0011 0.0016 0.0056 0.0026 0.0014 0.004 0.0018 0.0033
Bud 0.0028 0.0037 0.0048 0.0007 0.0008 0.0034 0.0006 0.0008 0.0008 0.0009 0.0002 0.0004 0.0003 0.0001 0.0012 0.001 0.0006 0.0001
Bud Neck 0.0012 0.0004 0.0004 0.0017 0.0005 0.001 0.0012 0.0002 0.0014 0.0006 0.0004 0.0006 0.0008 0.0008 0.0003 0.0006 0.0009 0.0009
Bud Periphery 0.0013 0.0019 0.0019 0.0004 0.001 0.0008 0.0005 0.0005 0.0009 0.0005 0.0001 0.0005 0.0002 0 0.001 0.0053 0.0006 0.0001
Bud Site 0.0117 0.0073 0.0069 0.0021 0.0002 0.0005 0.0061 0.0024 0.0047 0.0011 0.0008 0.0002 0.0044 0.0048 0.0048 0.0008 0.0017 0.0006
Cell Periphery 0.0004 0.0003 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0
Cytoplasm 0.8073 0.8693 0.851 0.8464 0.7691 0.8261 0.7708 0.9056 0.8614 0.8558 0.7397 0.87 0.8079 0.9321 0.918 0.7805 0.7046 0.8692
Cytoplasmic Foci 0.0272 0.0237 0.0231 0.0314 0.0327 0.0312 0.0325 0.019 0.0162 0.0314 0.0259 0.0176 0.0518 0.0129 0.0125 0.0247 0.0279 0.0478
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0094 0.0032 0.0021 0.0027 0.0051 0.0046 0.0051 0.0021 0.004 0.0025 0.003 0.0025 0.0049 0.0022 0.0018 0.0067 0.0011 0.0023
Endosome 0.0303 0.0268 0.0263 0.0486 0.0381 0.04 0.0275 0.0183 0.018 0.036 0.0475 0.0166 0.0333 0.0093 0.0198 0.0597 0.0568 0.0343
Golgi 0.0026 0.002 0.0016 0.0012 0.0015 0.0015 0.0084 0.0029 0.0018 0.0011 0.0023 0.0029 0.0071 0.0008 0.0011 0.01 0.0624 0.0096
Lipid Particles 0.001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0043 0.0001 0.0004 0.0002 0.003 0.0017 0.0048 0.0001 0.0002 0.0298 0.0297 0.0047
Mitochondria 0.0022 0.001 0.0017 0.0008 0.0044 0.0017 0.0033 0.0006 0.0027 0.0005 0.0009 0.0034 0.0019 0.0079 0.0041 0.0197 0.0755 0.007
None 0.091 0.0482 0.0677 0.0409 0.1356 0.0761 0.1014 0.038 0.0784 0.0545 0.1399 0.0763 0.06 0.0229 0.0235 0.0365 0.0095 0.0063
Nuclear Periphery 0.0009 0.0007 0.0006 0.0012 0.001 0.0006 0.0008 0.0005 0.0009 0.0007 0.001 0.0002 0.0009 0.0004 0.0008 0.0022 0.0005 0.0004
Nucleolus 0.0005 0.0002 0.0006 0.0003 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0003 0 0.0004 0 0.0003 0.0002 0.0022 0.0001
Nucleus 0.0021 0.003 0.0028 0.0025 0.0021 0.0026 0.0009 0.0009 0.001 0.0012 0.0022 0.0005 0.0011 0.0005 0.0017 0.0013 0.0012 0.0006
Peroxisomes 0.0002 0.0002 0.0004 0.0003 0.0004 0.0002 0.0055 0.0002 0.0002 0.0002 0.0258 0.0021 0.0055 0.0002 0.0002 0.0052 0.0105 0.0072
Punctate Nuclear 0.0015 0.0007 0.0009 0.002 0.0005 0.0021 0.0041 0.0002 0.0003 0.0003 0.0016 0.0002 0.0031 0.0001 0.0003 0.0005 0.0005 0.0032
Vacuole 0.0049 0.0057 0.0051 0.0134 0.0053 0.0056 0.004 0.0032 0.0046 0.0107 0.0033 0.002 0.005 0.0019 0.0053 0.0083 0.0092 0.0016
Vacuole Periphery 0.0009 0.001 0.0009 0.0028 0.0014 0.0009 0.0006 0.0006 0.0007 0.0017 0.0007 0.0005 0.0008 0.0003 0.0011 0.0026 0.0024 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.0897 49.5252 37.7706 26.8912 41.9008 52.2943 45.9019 47.4099 37.7339 37.8811
Translational Efficiency 1.1119 0.8734 0.8852 1.1847 0.9828 0.9451 0.8864 1.008 0.9255 1.2247

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
224 1746 332 1276 1442 1326 2336 1197 1666 3072 2668 2473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.64 773.31 944.56 925.05 732.12 838.03 919.57 898.79 735.01 801.25 922.68 912.34
Standard Deviation 95.50 120.43 95.42 119.81 122.92 120.38 114.51 121.06 119.83 124.60 112.61 121.13
Intensity Change Log 2 — 0.037171 0.325767 0.295656 — 0.194922 0.328879 0.295904 — 0.117060 0.327301 0.295778

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000279 0.000254 0.000681 0.000142 0.000320 0.000088 0.000308 0.000144 0.000297 0.000108 0.000500
Bud Neck 0.007820 0.011489 0.001650 0.002782 0.007090 0.012590 0.003766 0.004443 0.007188 0.011964 0.003503 0.003586
Bud Site 0.004759 0.029069 0.007332 0.025572 0.008267 0.032430 0.012617 0.018426 0.007796 0.030520 0.011960 0.022113
Cell Periphery 0.000241 0.000117 0.000297 0.000094 0.000145 0.000150 0.000063 0.000173 0.000158 0.000131 0.000092 0.000133
Cytoplasm 0.521743* 0.555906* 0.466392* 0.632029* 0.510704* 0.517356* 0.773546* 0.516162* 0.512188* 0.539266* 0.735325* 0.575946*
Cytoplasmic Foci 0.295440* 0.244534* 0.085988 0.037105 0.250615* 0.242467* 0.033586 0.059916 0.256642* 0.243642* 0.040107 0.048146
Eisosomes 0.000219 0.000071 0.000119 0.000028 0.000102 0.000090 0.000011 0.000066 0.000117 0.000079 0.000025 0.000046
Endoplasmic Reticulum 0.001176 0.001657 0.002673 0.001088 0.003796 0.001176 0.001913 0.001188 0.003444 0.001449 0.002008 0.001136
Endosome 0.028237 0.029714 0.014100 0.005757 0.033074 0.043989 0.003644 0.016241 0.032423 0.035875 0.004946 0.010832
Golgi 0.003817 0.015014 0.000179 0.002975 0.007426 0.014857 0.000584 0.003404 0.006941 0.014947 0.000534 0.003183
Lipid Particles 0.009677 0.002995 0.004712 0.001016 0.009755 0.002492 0.000461 0.001952 0.009744 0.002778 0.000990 0.001469
Mitochondria 0.000411 0.004962 0.000109 0.001884 0.003567 0.003251 0.000312 0.005078 0.003142 0.004224 0.000287 0.003430
Mitotic Spindle 0.000206 0.001558 0.000271 0.030188 0.001844 0.000920 0.004659 0.011115 0.001624 0.001283 0.004113 0.020956
None 0.001627 0.001632 0.001150 0.004156 0.005145 0.000272 0.001979 0.005766 0.004672 0.001045 0.001876 0.004935
Nuclear Periphery 0.000219 0.000141 0.001078 0.000552 0.000368 0.000080 0.000272 0.000518 0.000348 0.000114 0.000372 0.000535
Nuclear Periphery Foci 0.000960 0.000441 0.005618 0.001722 0.000789 0.000188 0.000740 0.001174 0.000812 0.000332 0.001347 0.001457
Nucleolus 0.000513 0.000681 0.000805 0.000157 0.001237 0.000270 0.000190 0.000836 0.001140 0.000503 0.000266 0.000486
Nucleus 0.048873 0.014227 0.160505* 0.048647 0.031822 0.007664 0.055045 0.062871 0.034115 0.011394 0.068168 0.055532
Peroxisomes 0.002532 0.003332 0.000608 0.000953 0.002487 0.003369 0.000355 0.001484 0.002493 0.003348 0.000387 0.001210
Vacuole 0.070195 0.075627 0.245807* 0.200759* 0.116410 0.109494 0.105497 0.285345* 0.110197 0.090245 0.122957 0.241701*
Vacuole Periphery 0.001179 0.006556 0.000351 0.001857 0.005214 0.006575 0.000671 0.003537 0.004671 0.006564 0.000631 0.002670

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.41 -2.95 -1.81 -1.06 -0.76 -4.34 2.82 -2.16 1.08 -3.30 -5.60 1.37 -2.48 -0.64 -2.80
Bud Neck -1.81 3.17 2.82 10.60 -2.64 -5.39 5.90 5.20 8.73 -0.64 -6.18 6.63 7.74 13.49 -0.01
Bud Site -14.81 -1.04 -4.69 6.66 -2.51 -13.01 -0.93 -3.37 7.26 -2.42 -17.85 -1.17 -4.60 9.78 -3.05
Cell Periphery 6.26 -0.78 9.28 7.95 4.60 -0.17 7.67 0.60 0.60 -2.91 2.41 6.20 4.58 2.33 -0.88
Cytoplasm -2.11 4.70 0.82 4.56 -4.97 -0.67 -25.17 0.55 1.13 22.25 -3.37 -21.59 0.68 4.27 23.21
Cytoplasmic Foci 4.09 17.58 23.19 43.00 10.06 1.15 42.33 36.12 32.96 -8.37 2.25 45.73 45.31 53.36 -1.05
Eisosomes 6.48 4.14 8.64 6.47 11.56 1.42 15.36 6.95 5.21 -12.65 5.22 15.13 12.34 7.91 -7.22
Endoplasmic Reticulum -1.15 -5.86 1.28 2.15 7.93 4.69 3.29 5.04 0.86 0.84 3.95 2.83 5.43 2.23 1.61
Endosome -0.42 4.21 7.68 17.29 11.20 -4.32 22.22 11.61 13.02 -6.72 -1.93 22.29 18.18 20.21 -4.16
Golgi -10.18 4.68 2.99 14.61 -1.39 -7.75 14.08 6.28 11.80 -2.73 -11.21 14.73 8.82 18.61 -2.69
Lipid Particles 4.88 3.43 6.48 7.13 9.27 8.33 11.12 9.48 2.59 -3.90 9.10 11.68 11.31 6.57 -1.29
Mitochondria -7.95 6.34 -0.77 6.13 -1.51 0.33 4.04 0.14 -0.12 -3.03 -1.32 4.09 1.28 3.27 -3.26
Mitotic Spindle -2.92 -1.16 -7.55 -6.97 -6.80 1.21 -2.87 -3.50 -4.21 -1.76 0.51 -3.01 -7.52 -8.10 -5.62
None -0.02 0.88 -1.40 -1.58 -3.03 4.13 2.80 0.63 -2.87 -1.68 3.42 2.86 1.02 -3.14 -2.38
Nuclear Periphery 2.17 -12.05 -14.80 -15.91 2.63 4.89 -1.10 -1.24 -5.09 -0.66 4.53 -3.60 -6.25 -13.35 -4.46
Nuclear Periphery Foci 2.92 -8.51 -2.98 -5.95 7.93 6.95 0.22 -1.17 -3.96 -1.30 4.59 -5.69 -3.16 -6.52 1.99
Nucleolus -0.98 -1.00 3.45 3.25 3.36 4.23 4.73 2.04 -1.76 -2.19 3.01 4.60 3.45 0.75 -1.47
Nucleus 7.43 -9.29 -4.05 -20.71 7.87 13.35 -14.66 -7.44 -16.24 3.02 13.56 -16.88 -11.79 -25.74 5.12
Peroxisomes -1.42 3.54 3.64 10.80 0.69 -1.59 4.47 2.66 7.46 -4.53 -1.85 5.01 3.82 12.78 -4.16
Vacuole -0.91 -15.40 -27.75 -30.84 -2.62 1.11 -5.29 -23.53 -23.84 -20.31 4.78 -10.47 -33.49 -39.17 -24.44
Vacuole Periphery -11.16 5.88 2.20 11.82 -1.62 -2.28 12.37 4.34 5.86 -3.43 -4.12 12.61 7.51 11.70 -3.43
Standard name
Human Ortholog
Description Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Oca1

Oca1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oca1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available