Standard name
Human Ortholog
Description Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0.09 0 0 0 0 – 0 0 0 0 0.08 0 0 0 0 0 0.11 0.13 0.07 0.12 0.17 0.24
Bud 0 0 0.06 0 0.05 0.13 0.08 – 0.12 0.15 0.12 0.15 0.06 0 0.07 0.06 0.06 0.06 0 0 0 0 0 0
Bud Neck 0 0 0.13 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0.12 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.54 0.43 0.65 0.59 0.39 0.32 – 0.19 0.19 0.17 0.17 0.59 0.55 0.42 0.05 0 0.07 0.16 0.18 0.09 0.14 0.12 0.09
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.11 0 0.06 0 0.1 0 0.15 – 0 0.08 0.06 0 0.06 0.1 0.18 0.13 0.22 0.14 0.19 0.15 0.17 0.16 0.12 0.11
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.2 0 0.08 0 0.5 0.12 – 0.59 0.47 0.57 0.6 0 0 0 0.09 0.09 0.07 0.25 0.22 0.3 0.2 0.25 0.15
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0.1 0 0 0.06 0.06 0 0.25 – 0.12 0.19 0.14 0.15 0.09 0.14 0.23 0.44 0.3 0.55 0.14 0.12 0.21 0.2 0.19 0.26
SpindlePole 0.16 0.09 0.44 0.2 0.27 0.16 0.36 – 0.16 0.32 0.25 0.23 0.24 0.25 0.28 0.41 0.61 0.43 0.07 0.07 0.08 0.09 0.07 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 14 7 1 0 2 3 – 0 5 0 7 21 19 18 4 0 0 37 27 8 43 86 149
Bud 7 5 5 5 9 20 26 – 19 29 23 29 16 21 32 7 7 7 3 3 0 3 5 8
Bud Neck 3 0 11 5 4 4 8 – 3 2 3 5 8 11 5 2 6 1 4 8 0 14 14 21
Bud Site 0 3 0 1 0 7 9 – 7 8 5 11 1 5 2 3 4 15 – – – – – –
Cell Periphery 0 1 0 1 0 0 2 – 0 2 3 0 0 1 2 0 0 0 0 1 0 2 4 3
Cytoplasm 155 80 35 102 104 62 108 – 31 37 32 32 156 284 204 6 2 8 54 35 11 50 58 53
Endoplasmic Reticulum 2 8 1 0 0 4 0 – 1 1 1 4 2 4 8 2 0 0 0 1 0 1 4 2
Endosome 28 4 5 6 18 2 49 – 1 15 11 2 16 51 90 15 27 17 64 30 23 58 63 69
Golgi 3 1 0 0 0 0 0 – 0 0 0 0 0 1 0 3 1 2 5 2 1 1 4 5
Mitochondria 29 29 0 12 3 79 42 – 94 93 109 115 8 6 5 10 11 9 82 43 40 72 127 89
Nucleus 0 1 0 1 1 4 4 – 6 2 2 1 0 0 0 3 7 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 2 0 0 0 0 0 1 3 2 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 1 – 1 1 1 2 0 0 0 3 3 7 0 0 0 0 0 0
Peroxisomes 25 5 0 9 11 5 84 – 19 37 27 28 24 73 111 51 38 67 47 23 27 74 94 157
SpindlePole 40 13 36 32 47 25 120 – 25 62 48 45 63 129 139 47 76 53 22 14 10 34 36 39
Vac/Vac Membrane 5 1 0 0 2 2 6 – 1 1 1 1 0 2 3 1 2 0 4 7 0 2 4 3
Unique Cell Count 257 147 82 157 176 157 336 159 196 190 193 264 518 488 116 125 122 332 203 132 364 511 613
Labelled Cell Count 298 165 100 175 199 218 462 210 295 266 282 315 607 620 160 186 186 332 203 132 364 511 613


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.9 4.9 4.8 5.3 3.8 4.7 – 4.1 4.3 4.1 4.2 4.5 4.4 4.9 6.4 6.3 6.5 3.9 4.2 4.3
Std Deviation (1e-4) 0.8 0.6 1.5 2.2 1.8 1.7 1.8 – 1.6 1.5 1.4 1.2 1.1 0.9 1.1 1.4 1.2 1.5 1.2 1.6 1.3
Intensity Change (Log2) – – – -0.02 0.12 -0.36 -0.05 – -0.26 -0.19 -0.24 -0.23 -0.11 -0.16 -0.01 0.38 0.36 0.4 -0.33 -0.21 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5052 -1.0525 -1.4507 -1.4538 -1.4039 -1.2502 -0.0717 0.4504 0.354 0.5999 0.317 0.5823 2.7382 1.9341 2.0488 3.3364 2.2022 1.9146
Actin 0.0198 0.0152 0.0219 0.0086 0.0105 0.0069 0.0078 0.025 0.0148 0.0192 0.0096 0.0167 0.0826 0.0463 0.0509 0.0072 0.0119 0.0054
Bud 0.0003 0.002 0.0005 0.0003 0.0002 0.0005 0.0006 0.0034 0.0026 0.0012 0.0004 0.0003 0.0007 0.0019 0.001 0.0003 0.0037 0.0002
Bud Neck 0.0023 0.0043 0.0324 0.0019 0.0128 0.0067 0.0039 0.0064 0.0083 0.0295 0.0206 0.0099 0.0043 0.0027 0.0288 0.0012 0.0012 0.0629
Bud Periphery 0.0006 0.0027 0.001 0.0005 0.0005 0.0007 0.0015 0.0061 0.0049 0.0026 0.0009 0.0007 0.0011 0.002 0.0015 0.0011 0.0124 0.0004
Bud Site 0.0023 0.0519 0.0105 0.001 0.0014 0.0022 0.0127 0.0421 0.0232 0.0106 0.0024 0.0003 0.0029 0.0708 0.0219 0.0004 0.0042 0.0006
Cell Periphery 0.0003 0.0007 0.0004 0.0001 0.0001 0.0001 0.0004 0.0008 0.0003 0.0003 0.0001 0.0001 0.0006 0.001 0.0004 0.0002 0.0004 0.0003
Cytoplasm 0.0294 0.0549 0.0254 0.0126 0.0081 0.0282 0.0296 0.0667 0.0435 0.0541 0.0285 0.0304 0.041 0.11 0.0436 0.0399 0.0457 0.0618
Cytoplasmic Foci 0.3788 0.3348 0.3057 0.3051 0.3606 0.4075 0.3488 0.2975 0.2998 0.2914 0.3662 0.3576 0.2663 0.2103 0.263 0.2975 0.4061 0.422
Eisosomes 0.0006 0.0008 0.0009 0.0002 0.0007 0.0003 0.0002 0.0007 0.0006 0.0002 0.0001 0.0002 0.0009 0.0009 0.0008 0.0009 0.0004 0.0002
Endoplasmic Reticulum 0.0011 0.0016 0.0008 0.0002 0.0006 0.0004 0.001 0.0014 0.0011 0.0006 0.0009 0.0005 0.0056 0.0007 0.0009 0.0005 0.0035 0.0012
Endosome 0.0603 0.0326 0.021 0.0253 0.0919 0.0242 0.0771 0.0555 0.0335 0.1126 0.0571 0.0359 0.0404 0.0305 0.0361 0.0168 0.057 0.0321
Golgi 0.0141 0.0078 0.0071 0.0083 0.0143 0.0071 0.0156 0.0129 0.0095 0.0177 0.0118 0.0092 0.0226 0.0133 0.0226 0.0041 0.0103 0.0057
Lipid Particles 0.0487 0.0479 0.0229 0.0246 0.1077 0.0426 0.0469 0.0177 0.015 0.0417 0.041 0.0172 0.0425 0.0264 0.0253 0.0347 0.0379 0.0262
Mitochondria 0.0069 0.0049 0.0093 0.0033 0.0209 0.006 0.0106 0.0061 0.0089 0.007 0.0091 0.0081 0.0053 0.0048 0.0101 0.0046 0.029 0.0081
None 0.1982 0.2863 0.2974 0.1843 0.0906 0.2461 0.1982 0.2198 0.2364 0.1551 0.1438 0.22 0.3235 0.3065 0.3568 0.4045 0.1997 0.2401
Nuclear Periphery 0.0008 0.0011 0.0006 0.0004 0.0016 0.0004 0.0007 0.0005 0.0008 0.0007 0.0018 0.0003 0.0114 0.0002 0.0003 0.0002 0.0062 0.0008
Nucleolus 0.0012 0.0026 0.0011 0.0142 0.0014 0.001 0.0017 0.0012 0.0007 0.004 0.0005 0.0004 0.0007 0.0004 0.0005 0.0005 0.0016 0.0007
Nucleus 0.0022 0.003 0.0009 0.0164 0.0007 0.001 0.0011 0.0011 0.0009 0.0032 0.0007 0.0005 0.0022 0.0003 0.0004 0.0003 0.0048 0.0007
Peroxisomes 0.2202 0.1195 0.2315 0.3836 0.257 0.2025 0.2282 0.2172 0.2839 0.2303 0.2914 0.2773 0.127 0.1661 0.1316 0.1809 0.1499 0.1166
Punctate Nuclear 0.0094 0.0238 0.0071 0.0073 0.0161 0.0138 0.0104 0.0158 0.0097 0.0146 0.01 0.0133 0.0156 0.0038 0.0021 0.0028 0.0079 0.0112
Vacuole 0.0021 0.0013 0.0013 0.0016 0.0017 0.0014 0.0025 0.0018 0.0011 0.0026 0.0022 0.0008 0.0017 0.001 0.001 0.0012 0.0038 0.0023
Vacuole Periphery 0.0004 0.0003 0.0003 0.0002 0.0007 0.0004 0.0005 0.0004 0.0003 0.0008 0.0009 0.0003 0.0008 0.0002 0.0004 0.0002 0.0025 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0801 5.4496 2.6959 7.3938 5.2693 4.0619 3.6734 10.537 10.3533 6.5901
Translational Efficiency 1.1208 1.3865 2.3504 1.4117 1.9255 1.8506 1.9139 0.9836 1.1293 1.9399

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1111 365 1322 393 1339 2269 583 148 2450 2634 1905 541

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 618.89 674.61 839.17 855.24 660.77 733.02 853.01 924.75 641.78 724.93 843.41 874.26
Standard Deviation 72.03 103.43 98.68 118.62 76.33 85.77 109.67 86.89 77.28 90.70 102.37 115.10
Intensity Change Log 2 — 0.124371 0.439280 0.466646 — 0.149704 0.368414 0.484915 — 0.137508 0.403122 0.476109

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001320 0.003673 0.024423 0.036685 0.001653 0.002955 0.064477 0.038907 0.001502 0.003054 0.036681 0.037293
Bud Neck 0.040430 0.043915 0.020586 0.009595 0.022056 0.048158 0.012127 0.006940 0.030388 0.047570 0.017997 0.008869
Bud Site 0.006426 0.022867 0.051305 0.042507 0.006389 0.022414 0.066857 0.025659 0.006406 0.022476 0.056064 0.037898
Cell Periphery 0.000364 0.000289 0.000327 0.000392 0.000251 0.000265 0.000522 0.000661 0.000303 0.000268 0.000387 0.000465
Cytoplasm 0.235263* 0.151459* 0.117408* 0.102285* 0.312326* 0.261772* 0.081093 0.117130* 0.277381* 0.246486* 0.106295* 0.106346*
Cytoplasmic Foci 0.405681* 0.365882* 0.182693* 0.193894* 0.381129* 0.335191* 0.157922 0.232785* 0.392262* 0.339444* 0.175112 0.204533*
Eisosomes 0.001021 0.000446 0.000223 0.000346 0.000545 0.000602 0.000390 0.000589 0.000761 0.000580 0.000274 0.000413
Endoplasmic Reticulum 0.000667 0.001776 0.003641 0.001751 0.000691 0.000724 0.003588 0.003245 0.000680 0.000870 0.003625 0.002160
Endosome 0.023867 0.064212 0.014017 0.042283 0.016628 0.025492 0.013961 0.050532 0.019911 0.030858 0.014000 0.044540
Golgi 0.017111 0.028223 0.003026 0.005619 0.010763 0.019615 0.003401 0.007012 0.013642 0.020808 0.003141 0.006000
Lipid Particles 0.042422 0.029534 0.012974 0.019595 0.031881 0.019974 0.012575 0.013924 0.036661 0.021298 0.012852 0.018043
Mitochondria 0.029163 0.033321 0.002004 0.001643 0.011628 0.032477 0.000899 0.000278 0.019579 0.032594 0.001666 0.001270
Mitotic Spindle 0.000703 0.004799 0.052290 0.052499 0.002301 0.005845 0.028107 0.006355 0.001576 0.005700 0.044889 0.039876
None 0.003063 0.001931 0.001285 0.001432 0.002740 0.002431 0.000564 0.000334 0.002886 0.002362 0.001064 0.001132
Nuclear Periphery 0.000536 0.000324 0.000808 0.000380 0.000422 0.000332 0.000284 0.000347 0.000473 0.000331 0.000648 0.000371
Nuclear Periphery Foci 0.000261 0.000463 0.003712 0.002270 0.000611 0.000189 0.002483 0.003797 0.000452 0.000227 0.003336 0.002688
Nucleolus 0.001229 0.001926 0.002214 0.000708 0.001036 0.001054 0.000571 0.000320 0.001124 0.001175 0.001711 0.000602
Nucleus 0.088121 0.051898 0.121774 0.049664 0.104929 0.089924 0.056345 0.040962 0.097307 0.084654 0.101750 0.047283
Peroxisomes 0.078416 0.140602* 0.342833* 0.331340* 0.066904 0.091032 0.443735* 0.303942* 0.072124 0.097901 0.373713* 0.323845*
Vacuole 0.022544 0.049420 0.042215 0.104599 0.024146 0.037607 0.049957 0.146126 0.023419 0.039244 0.044584 0.115960
Vacuole Periphery 0.001392 0.003041 0.000240 0.000514 0.000970 0.001947 0.000141 0.000152 0.001161 0.002099 0.000210 0.000415

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.22 -11.52 -5.94 -5.36 -1.34 -2.25 -12.10 -3.62 -3.46 2.76 -3.73 -16.28 -6.92 -6.52 0.72
Bud Neck -0.55 4.79 8.64 6.52 5.62 -9.50 3.21 6.08 15.63 2.67 -6.74 4.52 9.79 16.69 6.09
Bud Site -5.29 -15.38 -5.61 -2.32 2.47 -9.10 -11.94 -4.54 -0.46 6.63 -10.50 -19.50 -6.63 -2.54 4.81
Cell Periphery 1.40 -0.00 -0.28 -1.22 -0.30 -0.51 -5.35 -2.19 -2.11 -0.29 1.39 -4.37 -2.10 -2.60 -0.32
Cytoplasm 6.59 12.57 9.76 2.41 0.93 5.52 20.45 9.56 7.14 -1.58 4.29 23.69 15.50 12.64 -0.11
Cytoplasmic Foci 2.97 26.69 17.68 11.09 -1.50 5.66 24.31 10.02 7.41 -4.13 8.12 35.42 20.20 14.71 -3.43
Eisosomes 5.59 9.81 7.40 1.16 -2.09 -0.80 1.89 -0.07 0.39 -1.07 2.92 9.41 5.35 2.85 -2.12
Endoplasmic Reticulum -2.44 -12.13 -5.81 -1.24 6.06 -0.32 -9.07 -3.34 -3.30 1.92 -1.85 -15.03 -6.37 -5.66 5.99
Endosome -6.29 4.19 -2.71 3.52 -4.74 -4.62 1.60 -2.67 -1.70 -3.02 -5.94 3.74 -4.29 -1.72 -5.63
Golgi -2.41 9.20 7.56 5.58 -2.28 -6.37 8.42 2.91 7.23 -1.32 -5.15 12.44 8.32 12.74 -2.57
Lipid Particles 2.69 9.16 6.13 2.19 -1.16 4.98 7.26 5.00 1.70 -0.42 7.64 11.32 6.97 1.24 -1.27
Mitochondria -0.65 10.20 10.47 6.38 2.31 -9.30 8.40 9.07 17.21 7.55 -5.84 12.68 13.11 17.76 2.91
Mitotic Spindle -2.35 -13.19 -6.21 -5.59 0.72 -2.88 -5.89 -1.46 0.06 4.89 -4.55 -14.17 -6.17 -5.46 1.64
None 0.72 1.72 1.81 1.11 0.60 0.54 3.79 4.08 3.91 1.16 0.84 3.21 3.02 2.34 0.70
Nuclear Periphery 1.35 -4.55 -1.08 -3.98 5.14 0.63 -1.05 -1.19 -1.91 -0.35 1.15 -5.46 -1.91 -3.17 4.59
Nuclear Periphery Foci -3.17 -8.19 -5.69 -5.27 2.62 2.39 -6.12 -4.96 -5.73 -0.12 2.17 -9.40 -6.87 -7.40 2.39
Nucleolus -1.55 -4.02 0.43 1.62 4.08 -0.15 1.68 3.02 2.85 1.43 -1.14 -3.95 0.98 1.66 4.17
Nucleus 4.60 -6.64 3.74 -0.51 8.58 2.90 1.93 4.44 3.05 2.37 3.03 -4.55 6.12 4.14 8.60
Peroxisomes -5.08 -20.28 -10.86 -6.19 0.96 -4.65 -20.05 -6.50 -5.57 4.18 -5.70 -28.68* -12.99 -11.06 3.13
Vacuole -5.78 -12.41 -11.80 -9.08 -7.98 -6.89 -9.99 -9.35 -8.65 -5.87 -9.36 -15.88 -14.87 -13.26 -9.78
Vacuole Periphery -3.86 8.97 5.40 6.15 -1.07 -4.47 7.15 7.09 9.41 -0.36 -4.90 10.85 6.37 8.85 -1.13
Standard name
Human Ortholog
Description Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation
Localization
Cell Percentages mitochondrion (10%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pex22

Pex22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available