Standard name
Human Ortholog
Description Protein of unknown function; mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0 0 0 0 0 0 0
Cytoplasm 0.93 0.98 0.96 0.94 0.94 0.85 0.82 0.71 0.71 0.7 0.63 0.62 0.97 0.97 0.97 0.86 0.82 0.86 0.94 0.92 0.85 0.81 0.81 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0.06 0.08 0.12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.21 0.09 0.38 0.39 0.47 0.58 0.55 0.61 0.63 0.06 0 0.07 0 0 0.06 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0.08 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2
Bud 0 0 3 2 2 8 5 7 10 7 11 15 3 2 7 1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 1 0 1 1
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 22 0 6 2 5 5 7 5 7 7 5 11 1 1 4 9 15 5 0 0 0 0 0 0
Cytoplasm 421 95 305 271 301 398 417 395 274 375 233 292 105 191 194 157 104 111 450 101 305 74 153 133
Endoplasmic Reticulum 12 0 6 2 3 4 3 5 1 5 1 5 0 2 1 13 6 9 6 2 11 5 15 20
Endosome 4 0 0 0 0 2 2 2 0 1 0 0 0 0 0 1 3 0 2 0 1 0 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 0 0 3 0 0 1 1 0
Mitochondria 1 2 17 61 30 177 199 260 224 294 226 299 6 3 14 5 3 8 2 0 24 1 4 2
Nucleus 1 0 1 2 0 4 6 7 0 0 0 5 0 0 0 3 4 4 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 17 1 3 0 6 2 10 24 6 7 4 14 0 0 1 16 17 10 6 2 12 4 5 7
Unique Cell Count 453 97 318 289 321 469 511 554 386 535 369 473 108 196 199 183 127 129 480 110 362 91 190 173
Labelled Cell Count 480 98 341 341 349 600 649 707 524 698 482 641 115 201 223 210 152 148 480 110 362 91 190 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 6.7 5.5 5.3 5.5 4.8 4.5 4.4 4.0 3.9 3.9 3.8 7.0 7.1 6.1 9.7 11.2 10.6 7.2 7.6 8.2
Std Deviation (1e-4) 0.9 0.8 0.6 0.8 1.2 1.1 1.2 1.0 0.9 1.1 0.9 1.1 1.2 1.1 1.0 1.8 2.6 2.0 1.4 1.3 1.2
Intensity Change (Log2) – – – -0.04 0.02 -0.19 -0.27 -0.31 -0.46 -0.47 -0.48 -0.54 0.36 0.37 0.16 0.83 1.03 0.96 0.4 0.48 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.3 0 0 0 0 0 0 0 0 0 0 1.9 4.5 0
Cytoplasm -1.2 -1.2 -4.9 -6.0 -8.8 -8.6 -9.1 -10.4 -11.0 0.6 0.9 1.0 -4.1 -4.9 -3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.8 1.9 0 0 0 0 0 0 0 0.1 -2.2 0.8 -1.4 -1.4 0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.8 0 0 0 1.9 0 0 0 4.4 5.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8176 4.2512 3.5389 3.3616 3.9328 3.9616 3.5683 5.3707 4.5771 4.1727 4.4075 4.7172 4.4771 5.7117 5.0047 5.6283 4.8194 5.2273
Actin 0.0012 0.0013 0.0039 0.0026 0.0004 0.0033 0.0346 0.0005 0.0263 0.0005 0.007 0.0011 0.0096 0.0035 0.0084 0.0082 0.0018 0.0018
Bud 0.0002 0.0002 0.001 0.0006 0.0003 0.0005 0.0009 0.0005 0.0006 0.0002 0.0008 0.0022 0.0006 0.001 0.0005 0.0204 0.0005 0.0003
Bud Neck 0.0004 0.0004 0.0016 0.0009 0.0013 0.0128 0.0014 0.0001 0.0011 0.0004 0.0005 0.0014 0.0003 0.0004 0.0003 0.0003 0.0004 0.0008
Bud Periphery 0.0003 0.0001 0.0012 0.0004 0.0001 0.0006 0.0012 0.0003 0.0006 0.0001 0.0006 0.0017 0.0008 0.0004 0.0002 0.0519 0.0002 0.0003
Bud Site 0.0009 0.0037 0.0136 0.0004 0.0001 0.0003 0.0102 0.0014 0.0054 0.0002 0.0011 0.0029 0.001 0.0205 0.001 0.0135 0.0014 0.0001
Cell Periphery 0.0016 0.0009 0.0012 0.0002 0.0001 0.0004 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0006 0.0003 0.0003 0.0018 0.0002 0.0001
Cytoplasm 0.8197 0.9507 0.8813 0.9178 0.7573 0.8086 0.6704 0.9211 0.8004 0.8819 0.8035 0.8979 0.7703 0.922 0.8612 0.6742 0.7912 0.8787
Cytoplasmic Foci 0.0098 0.0076 0.0189 0.0157 0.0305 0.0207 0.0445 0.0139 0.0264 0.0201 0.046 0.0196 0.0428 0.0176 0.0251 0.02 0.0394 0.0161
Eisosomes 0.0003 0.0001 0.0001 0 0 0 0.0005 0.0002 0.0002 0 0 0 0.0002 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0419 0.0114 0.0115 0.0053 0.0333 0.0318 0.0268 0.0019 0.0055 0.0033 0.002 0.0047 0.0129 0.0018 0.0078 0.0031 0.0022 0.0081
Endosome 0.0239 0.008 0.0296 0.0344 0.1483 0.0515 0.0599 0.0216 0.046 0.0609 0.0631 0.0263 0.0667 0.0179 0.0277 0.0119 0.0456 0.032
Golgi 0.0021 0.0014 0.0047 0.0033 0.0112 0.0086 0.0166 0.0021 0.0139 0.0131 0.0297 0.0034 0.0116 0.0029 0.0069 0.0021 0.0139 0.0026
Lipid Particles 0.0011 0.0007 0.0004 0.0001 0.0003 0.0009 0.0154 0.0001 0.0032 0.0008 0.0055 0.0004 0.0036 0.0001 0.0017 0.0002 0.0018 0.0001
Mitochondria 0.0007 0.0003 0.0085 0.0012 0.0044 0.0157 0.0127 0.0006 0.0085 0.0033 0.023 0.002 0.005 0.0003 0.0013 0.0006 0.0027 0.0009
None 0.0735 0.0078 0.0111 0.0041 0.0019 0.0347 0.064 0.0326 0.0484 0.0059 0.0064 0.031 0.0592 0.0081 0.0389 0.1884 0.0898 0.0549
Nuclear Periphery 0.0056 0.001 0.0011 0.0009 0.0007 0.0017 0.006 0.0002 0.0014 0.0005 0.0004 0.0004 0.0032 0.0003 0.0009 0.0003 0.0004 0.0003
Nucleolus 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0021 0 0.0001 0 0.0001 0.0001 0.0002 0 0 0.0001 0.0001 0
Nucleus 0.002 0.0013 0.0011 0.0017 0.0006 0.0016 0.0026 0.0005 0.0007 0.0007 0.0005 0.0006 0.0013 0.0005 0.0007 0.0005 0.0007 0.0004
Peroxisomes 0.0001 0.0001 0.0004 0.0002 0.0001 0.0002 0.0164 0.0001 0.0045 0.0002 0.0046 0.0017 0.0024 0.0002 0.0105 0.0002 0.0023 0.0004
Punctate Nuclear 0.0033 0.0005 0.0003 0.0002 0.0001 0.0006 0.0032 0.0001 0.0007 0.0001 0.0004 0.0003 0.0016 0.0002 0.0004 0.0002 0.0003 0.0001
Vacuole 0.0097 0.0022 0.0071 0.0083 0.0052 0.004 0.0074 0.0017 0.0046 0.0063 0.0029 0.0018 0.0046 0.0017 0.0051 0.0017 0.0042 0.0014
Vacuole Periphery 0.0013 0.0003 0.0015 0.0019 0.0039 0.0014 0.0025 0.0003 0.0011 0.0015 0.0017 0.0006 0.0017 0.0003 0.0008 0.0003 0.0009 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.8582 22.2551 19.4632 29.5956 23.8243 17.7727 29.7349 30.646 28.266 30.2373
Translational Efficiency 0.7217 0.9181 0.9115 0.7656 0.6883 0.8799 0.7349 0.6365 0.6819 0.5997

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
562 47 316 1377 1751 2148 88 834 2313 2195 404 2211

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 817.35 977.59 1324.70 990.70 858.14 909.53 1169.68 1035.43 848.23 910.99 1290.93 1007.57
Standard Deviation 99.31 130.58 151.15 152.20 101.57 115.12 150.84 152.71 102.53 115.89 164.08 153.93
Intensity Change Log 2 — 0.258276 0.696640 0.277494 — 0.083908 0.446829 0.270945 — 0.171603 0.574098 0.274144

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000281 0.000768 0.001449 0.000247 0.000237 0.000330 0.000478 0.000595 0.000248 0.000339 0.001238 0.000378
Bud Neck 0.003993 0.007004 0.002022 0.006143 0.005559 0.007138 0.001730 0.001473 0.005178 0.007135 0.001958 0.004382
Bud Site 0.020183 0.058732 0.026268 0.018808 0.019998 0.029594 0.019475 0.031886 0.020043 0.030218 0.024788 0.023741
Cell Periphery 0.000388 0.000214 0.000234 0.000300 0.000147 0.000117 0.000183 0.000125 0.000205 0.000119 0.000223 0.000234
Cytoplasm 0.553314* 0.443309* 0.764390* 0.428574* 0.489873* 0.435026* 0.564055* 0.727170* 0.505287* 0.435203* 0.720753* 0.541206*
Cytoplasmic Foci 0.298036* 0.282570* 0.074975 0.077338 0.321021* 0.291068* 0.146262 0.128860 0.315436* 0.290886* 0.090503 0.096772
Eisosomes 0.000110 0.000084 0.000014 0.000241 0.000082 0.000079 0.000041 0.000012 0.000089 0.000080 0.000020 0.000155
Endoplasmic Reticulum 0.007664 0.001425 0.002327 0.001385 0.005224 0.001994 0.002818 0.000455 0.005817 0.001981 0.002434 0.001034
Endosome 0.020751 0.066650 0.010201 0.021179 0.031975 0.053133 0.024353 0.009233 0.029248 0.053422 0.013284 0.016673
Golgi 0.023258 0.015171 0.003211 0.004826 0.029442 0.028082 0.000937 0.004680 0.027939 0.027806 0.002716 0.004771
Lipid Particles 0.005763 0.002533 0.002891 0.003322 0.004520 0.002334 0.003295 0.001548 0.004822 0.002338 0.002979 0.002653
Mitochondria 0.005368 0.000559 0.000082 0.001571 0.005974 0.005247 0.000030 0.000286 0.005827 0.005147 0.000071 0.001087
Mitotic Spindle 0.000292 0.000357 0.007624 0.017613 0.000636 0.003689 0.001307 0.000918 0.000553 0.003618 0.006248 0.011316
None 0.001452 0.000044 0.000602 0.002774 0.000592 0.000433 0.000259 0.000236 0.000801 0.000425 0.000528 0.001816
Nuclear Periphery 0.000116 0.000032 0.000246 0.000483 0.000066 0.000063 0.000331 0.000032 0.000078 0.000063 0.000264 0.000313
Nuclear Periphery Foci 0.000371 0.000376 0.009254 0.001556 0.000182 0.000139 0.011770 0.001858 0.000228 0.000144 0.009802 0.001670
Nucleolus 0.000157 0.000117 0.000070 0.000613 0.000160 0.000160 0.000111 0.000095 0.000159 0.000159 0.000079 0.000417
Nucleus 0.009768 0.003094 0.025823 0.070541 0.005910 0.004893 0.014980 0.005548 0.006848 0.004855 0.023461 0.046026
Peroxisomes 0.001466 0.004788 0.000511 0.002261 0.001697 0.002630 0.000772 0.001711 0.001641 0.002676 0.000568 0.002054
Vacuole 0.036931 0.103960 0.066188 0.336886* 0.063167 0.114811 0.205963* 0.081711 0.056792 0.114578 0.096634 0.240633*
Vacuole Periphery 0.010337 0.008212 0.001617 0.003338 0.013538 0.019038 0.000851 0.001568 0.012760 0.018806 0.001450 0.002670

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.62 -4.22 0.65 2.78 4.32 -0.98 -3.48 -3.72 -2.01 -0.87 -1.01 -4.51 -2.30 -0.28 3.99
Bud Neck -1.85 4.19 -1.79 1.02 -4.58 -2.81 7.90 9.26 15.58 1.44 -4.01 7.90 2.64 6.37 -3.91
Bud Site -3.02 -1.39 1.51 3.29 2.20 -6.29 0.46 -4.46 0.02 -2.96 -7.09 -1.32 -0.93 4.80 0.78
Cell Periphery 2.12 1.76 1.97 -0.38 0.68 1.94 -1.25 2.80 0.13 2.63 3.72 -1.35 0.67 -2.89 2.07
Cytoplasm 2.78 -10.73 8.76 0.15 19.11 6.35 -1.74 -21.70 -27.46 -5.75 8.76 -13.54 -4.26 -12.07 10.59
Cytoplasmic Foci 0.52 24.06 26.33 7.37 -0.19 4.59 12.70 28.44 25.29 1.11 4.11 35.26 45.22 39.07 -1.02
Eisosomes 1.21 6.19 -2.07 -2.79 -5.24 0.10 6.96 17.57 2.77 6.14 0.39 13.35 -1.47 -1.28 -4.86
Endoplasmic Reticulum 4.47 3.88 4.99 0.64 3.00 4.95 2.95 7.84 6.75 3.18 6.35 5.19 8.72 4.64 4.43
Endosome -3.36 4.77 1.62 3.60 -3.33 -10.25 2.92 15.84 24.37 4.04 -12.58 9.15 11.09 20.96 -0.69
Golgi 1.77 8.28 7.58 2.85 -0.79 0.96 29.96 14.89 13.08 -2.09 0.15 25.58 19.93 17.33 -1.52
Lipid Particles 3.14 3.35 3.70 -0.44 0.53 8.16 2.44 10.74 3.63 3.31 8.96 4.27 7.30 -0.24 2.08
Mitochondria 3.02 3.34 2.56 -1.65 -3.24 0.85 7.94 7.59 11.06 -1.90 0.86 8.41 6.91 8.84 -3.49
Mitotic Spindle -0.39 -5.65 -6.04 -6.00 -2.81 -3.60 -1.53 -0.89 3.23 1.06 -3.76 -5.52 -5.96 -3.78 -2.00
None 2.72 1.63 -0.69 -2.34 -1.72 0.59 1.52 1.60 1.10 0.14 1.45 1.28 -1.08 -1.74 -1.61
Nuclear Periphery 1.52 -3.20 -3.79 -5.94 -1.62 0.12 -4.19 1.74 2.36 4.59 0.84 -7.13 -4.73 -5.30 0.56
Nuclear Periphery Foci -0.03 -10.91 -2.19 -2.06 8.77 1.78 -5.58 -9.26 -9.45 4.66 3.38 -12.35 -4.68 -4.94 10.10
Nucleolus 0.51 4.22 -3.35 -3.22 -3.95 -0.01 3.63 6.22 4.96 0.77 -0.01 8.65 -3.04 -3.03 -3.93
Nucleus 3.21 -6.55 -12.04 -13.53 -5.52 2.03 -4.16 -1.10 -2.42 3.85 3.46 -8.67 -13.06 -13.84 -3.08
Peroxisomes -3.41 6.10 -1.75 2.80 -4.98 -4.95 7.16 0.54 4.50 -4.57 -5.71 9.45 -1.03 3.46 -6.32
Vacuole -4.03 -6.10 -38.39 -13.85 -23.35 -13.18 -7.52 -7.99 2.07 5.69 -15.74 -7.33 -33.55 -21.97 -13.30
Vacuole Periphery 0.77 7.27 6.31 2.13 -1.87 -4.84 17.87 15.49 18.67 -1.48 -5.70 16.39 15.70 18.17 -1.79
Standard name
Human Ortholog
Description Protein of unknown function; mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Nst1

Nst1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nst1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available